TWI615134B - 自體細胞多維成形裝置 - Google Patents

自體細胞多維成形裝置 Download PDF

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TWI615134B
TWI615134B TW105129810A TW105129810A TWI615134B TW I615134 B TWI615134 B TW I615134B TW 105129810 A TW105129810 A TW 105129810A TW 105129810 A TW105129810 A TW 105129810A TW I615134 B TWI615134 B TW I615134B
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module
autologous
forming device
axis
autologous cell
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歐耿良
詹育豪
洪千惠
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三鼎生物科技股份有限公司
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Priority to CN201610886822.3A priority patent/CN107815413A/zh
Priority to US15/702,551 priority patent/US20180072973A1/en
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Abstract

本發明揭露了一種自體細胞多維成形裝置，將一自體細胞客製化成一人工植體。本發明自體細胞多維成形裝置包含有一正壓室、一自體細胞培養模組、一供料模組、一多軸成型模組以及一植體維持模組。自體細胞藉由自體細胞培養模組培養後送至供料模組與高分子材料混合成混合自體細胞，多軸成型模組將混合自體細胞列印成人工植體並利用植體維持模組承載與保存。其中，上述之模組皆以密閉連通管連通並處於一氣體壓力大於外界氣壓之正壓室內。相較於習知技術，本發明可客製化人工植體並有效提升製備人工植體之良率。

Description

自體細胞多維成形裝置
本發明係關於一種成形裝置，並且特別地，關於一種自體細胞多維成形裝置
隨著醫療技術的提升，人工植體如人工敷料與人造器官的使用越來越頻繁。人工敷料常用於大範圍燙傷或術後之患者，用以取代受損之皮膚，保護傷口免受汙染物或細菌的感染及吸收自身滲液保持身體水分不致逃逸，並促進結締組織的生長。人造器官如人工電子耳或人工血管，可用以模擬並取代原有器官之功能，或以半支援方式與原有器官共同維持身體機能。
但是，人工植體也可能對人體造成不良反應，因異質物質造成人體產生排他性，產生額外之免疫互斥問題。故後期均採用自體細胞做為人工植體之基材。
自體細胞組織的多維成形方法過去係將成形用原料或細胞原料以裝填方式裝入料桶中，再置入多維成形裝置原料區，由原料夾持器擷取後，進行人工植體之細胞及組織成形。
過程中，往往因為人為的移動與器皿的更換等，使原料容易受到汙染而導致植體成型良率不高。且多維成形裝置皆處於開放 空間，此種習知流程讓人工植體的製造容易因人員或環境而造成汙染，進而導致人工植體的產品良率不高。因此，如何設計一種成形用之細胞原料與成形過程中皆不被外界所汙染的細胞組織的多維成形裝置，實為目前急迫解決之問題。
由此可見，上述習知技術仍有諸多缺失，實非一良善之設計，而亟待加以改良。有鑑於此，本發明將提出一種自體細胞多維成形裝置以利有效生產。
本發明之一範疇在於提供一種自體細胞多維成形裝置。根據本發明之一具體實施例，本發明自體細胞多維成形裝置，用以將一自體細胞客製化成一人工植體，其包含有一正壓室、一自體細胞培養模組、一供料模組、一多軸成型模組以及一植體維持模組。正壓室用以通入一壓力大於外界氣壓之氣體。自體細胞培養模組設置於正壓室內，其包含一培養器皿及一進料管道，培養器皿用以培養自體細胞成一培養後自體細胞，進料管道與培養器皿連接，並接收培養後自體細胞。供料模組設置於正壓室內，其包含一蠕動式供料器與一出料管道，蠕動式供料器連接進料管道，用以接收培養後自體細胞並與一高分子材料混合成一混合自體細胞，而出料管道與蠕動式供料器連接，用以接收混合自體細胞。多軸成型模組設置於正壓室內，多軸成型模組與出料管道連接，用以接收混合自體細胞並將混合自體細胞客製化列印成人工植體。植體維持模組設置於正壓室內，置於多軸成型模組下方，用以承載與保存人工植體。其中，進料管道與出料管道均 得為一密閉式輸送管道。
再者，進料管道與培養器皿之連接、蠕動式供料器與進料管道之連接、出料管道與蠕動式供料器之連接以及多軸成型模組與出料管道之連接均得為一密閉式連接。
多軸成型模組包含一列印注料頭、一圖型接收元件及一六軸移動手臂。列印注料頭與出料管道連接，用以輸入混合自體細胞並列印人工植體。該圖型接收元件用以接收一客製化圖案，以及六軸移動手臂以電連接於圖型接收元件，用以帶動列印注料頭之一客製化列印行程。
其中，六軸移動手臂之移動方式得為三維空間加上角度移動。
相較於習知技術，本發明自體細胞多維成形裝置利用一正壓室產生一不易被汙染之生產環境，並利用密閉式連接方式將從自體細胞原料至人工植體成品之生產過程全程與外界做隔離，再利用六軸成型模組做一客製化自體細胞多維列印，以得到高良率之客製化人工植體，再者，亦可利用植體維持裝置做一彈性時間與地點之人工植體的使用。
關於本發明之優點與精神可以藉由以下的發明詳述以及所附圖式得到進一步的了解。
1‧‧‧自體細胞多維成形裝置
12‧‧‧正壓室
14‧‧‧自體細胞培養模組
16‧‧‧供料模組
18‧‧‧多軸成型模組
19‧‧‧植體維持模組
141‧‧‧培養器皿
142‧‧‧進料管道
143‧‧‧馬達
161‧‧‧蠕動式供料器
162‧‧‧出料管道
181‧‧‧列印注料頭
182‧‧‧圖案接收元件
183‧‧‧六軸移動手臂
1411‧‧‧培養皿蓋
1411A‧‧‧細胞培養空間
1411B‧‧‧墊圈
1412‧‧‧培養皿座
1412A‧‧‧連通道I
1412B‧‧‧通氣道
1413‧‧‧傳動軸
1413A‧‧‧連通道II
1414‧‧‧軸承組
1415‧‧‧固定蓋組
1416‧‧‧傳動齒輪
1417‧‧‧迴轉接頭
1417A‧‧‧連通道III
圖一係繪示本發明之自體細胞多維成形裝置之一具體實施例之示意圖。
圖二係繪示本發明之自體細胞多維成形裝置之一具體實施例之功能方塊圖。
圖三係繪示本發明之自體細胞多維成形裝置之一具體實施例之培養器皿之示意圖。
為使本發明之目的、技術方案及優點更加清楚明白，以下參照附圖並舉實施例，對本發明作進一步詳細說明。
請參閱圖一及圖二，圖一係繪示本發明之自體細胞多維成形裝置1之一具體實施例之示意圖，圖二係繪示本發明之自體細胞多維成形裝置1之一具體實施例之功能方塊圖。在本發明之一實施例中，本發明自體細胞多維成形裝置1包含有一正壓室12、一自體細胞培養模組14、一供料模組16、一多軸成型模組18以及一植體維持模組19。正壓室12用以通入一壓力大於外界氣壓之氣體。自體細胞培養模組14設置於正壓室12內，其包含一培養器皿141及一進料管道142，培養器皿141用以培養自體細胞成一培養後自體細胞，進料管道142與培養器皿141連接，並接收培養後自體細胞。供料模組16設置於正壓室12內，其包含一蠕動式供料器161與一出料管道162，蠕動式供料器161連接進料管道142，用以接收培養後自體細胞並與一高分子材料混合成一混合自體細胞，而出料管道162與蠕動式供料器161連接，用以接收混合自體細胞。多軸成型模組18設置於正壓室12內，多軸成型模組18與出料管道162連接，用以接收混合自體細胞並將混合自體細胞客製化列印成人工植體。植體維持模組19設置於正壓室12內，置於多軸成型模組18下 方，用以承載與保存人工植體。其中，進料管道142與出料管道162均得為一密閉式輸送管道。
再者，進料管道142與培養器皿141之連接、蠕動式供料器161與進料管道142之連接、出料管道162與蠕動式供料器161之連接以及多軸成型模組18與出料管道162之連接均得為一密閉式連接。
其中，正壓室12通入之氣體得為一適合培養自體細胞之氣體。
自體細胞培養模組14還包含一馬達143，馬達143連接於培養器皿141，用以帶動培養器皿141使培養器皿141產生一離心旋轉。
於實際應用中，適當的氣體與離心旋轉方式可促進自體細胞加速成長與分離該培養後自體細胞。
請參閱圖三，圖三係繪示本發明之自體細胞多維成形裝置1之一具體實施例之培養器皿141之示意圖。培養皿蓋1411與培養皿座1412相結合以形成一細胞培養空間1411A，兩者間藉由一墊圈1411B形成密合狀態。培養皿座1412底部與傳動軸1413結合，兩者更以連通道I(1412A)與連通道H(1413A)相通，使培養後自體細胞得以送出培養器皿141。當培養後自體細胞送出細胞培養空間1411A時，通氣道1412B用以讓細胞培養空間1411A保持壓力平衡。傳動軸1413之適當部位安置一軸承組1414與固定蓋組1415，用以使傳動軸1413得以轉動，進而使培養器皿141中的自體細胞獲得無重力環境，以利自體細胞得以快速成長。傳動軸1413更與傳動齒輪1416結合，以獲得轉動所需的動力。傳動軸1413尾端與一迴轉接頭1417相連接，兩者間利用連通道II(1413A) 與連通道III(1417A)相互連通，使培養器皿141中的培養後自體細胞可由進料管道142送至蠕動式供料器161中。
於實際應用中，蠕動式供料器161的吸取動力可將培養後自體細胞由入料管道142吸入至蠕動式供料器161，並與適當之高分子材料混合成所需之混合自體細胞，而後蠕動式供料器161壓縮動力將混合自體細胞透過出料管道162送至多軸成型模組18中。
多軸成型模組18包含一列印注料頭181，列印注料頭181與出料管道162連接，用以輸入混合自體細胞並列印人工植體。
其中，多軸成型模組18得為一六軸成型模組，該六軸成型模組包含一圖型接收元件182及一六軸移動手臂183，圖型接收元件182用以接收一客製化圖案，六軸移動手臂183以電連接於圖型接收元件182，用以帶動列印注料頭181之一客製化列印行程。
再者，圖型接收元件182可利用藍芽、乙太區域網路、WiFi無線區域網路、無線行動通訊模組或其他可存取之移動式儲存設備接收所需之客製化圖案資料。
所述之六軸移動手臂183之移動方式得為三維空間加上角度移動。
於實際應用上，藉由多軸成型模組18的三維空間加角度移動，將混合自體細胞披覆於人工組織結構上，使細胞得以在結構上成形生長，而形成人工植體。
植體維持模組19具有一適合該人工植體之密閉儲存環境，其得為一移動式裝置。
藉由植體維持模組19位於正壓室12以產生一不易汙染的環境，並利用植體維持模組19自身之合適的無菌密閉儲存環境，可有效避免人工植體發生汙染狀況，以確保人工植體具有一定之活性與功效。於實際應用上，人工植體(如：人工敷料、人工血管、義乳)從製備完成到患者實際使用期間，往往需要等待患者到場的時間或將人工植體移至合適場地之移動，植體維持模組19可保存待使用之人工植體並送至偏遠地區或以隨身攜帶方式做為使用，使得人工植體在使用場合與時間上更為彈性。
相較於習知技術，本發明自體細胞多維成形裝置利用一正壓室產生一不易被汙染之生產環境，並利用密閉式連接方式將從自體細胞原料至人工植體成品之生產過程全程與外界做隔離，再利用六軸成型模組做一客製化自體細胞多維列印，以得到高良率之客製化人工植體。再者，亦可利用植體維持裝置做一彈性時間與地點之人工植體的使用。
藉由以上較佳具體實施例之詳述，係希望能更加清楚描述本發明之特徵與精神，而並非以上述所揭露的較佳具體實施例來對本發明之範疇加以限制。相反地，其目的是希望能涵蓋各種改變及具相等性的安排於本發明所欲申請之專利範圍的範疇內。
1‧‧‧自體細胞多維成形裝置
12‧‧‧正壓室
14‧‧‧自體細胞培養模組
16‧‧‧供料模組
18‧‧‧多軸成型模組
19‧‧‧植體維持模組
141‧‧‧培養器皿
142‧‧‧進料管道
143‧‧‧馬達
161‧‧‧蠕動式供料器
162‧‧‧出料管道
181‧‧‧列印注料頭
182‧‧‧圖案接收元件
183‧‧‧六軸移動手臂

Claims (10)

  1. 一種自體細胞多維成形裝置，用以將一自體細胞客製化成一人工植體，其包含有：一正壓室，用以通入一壓力大於外界氣壓之氣體；一自體細胞培養模組，設置於該正壓室內，其包含一培養器皿及一進料管道，該培養器皿用以培養該自體細胞成一培養後自體細胞，該進料管道與該培養器皿連接，並接收該培養後自體細胞；一供料模組，設置於該正壓室內，其包含一蠕動式供料器與一出料管道，該蠕動式供料器連接該進料管道，用以接收該培養後自體細胞並與一高分子材料混合成一混合自體細胞，而該出料管道與該蠕動式供料器連接，用以接收該混合自體細胞；一多軸成型模組，設置於該正壓室內，該多軸成型模組與該出料管道連接，用以接收該混合自體細胞並將該混合自體細胞客製化列印成該人工植體；以及一植體維持模組，設置於該正壓室內，置於該多軸成型模組下方，用以承載與保存該人工植體；其中，該進料管道與該出料管道均為一密閉式輸送管道；該蠕動式供料器提供一吸取動力以將該培養後自體細胞由該培養器皿沿該進料管道進入該蠕動式供料器中。
  2. 如申請專利範圍第1項所述之自體細胞多維成形裝置，其中該進料管道與該培養器皿之連接、該蠕動式供料器與該進料管道之連接、該出料管道與該蠕動式供料器之連接以及該多軸成型模組與該出料管道之連接均為一密閉式連接。
  3. 如申請專利範圍第1項所述之自體細胞多維成形裝置，其中該正壓室通入之該氣體為一適合培養該自體細胞之氣體。
  4. 如申請專利範圍第1項所述之自體細胞多維成形裝置，其中該自體 細胞培養模組包含一馬達，該馬達連接於該培養器皿，用以帶動該培養器皿使該培養器皿產生一離心旋轉。
  5. 如申請專利範圍第1項所述之自體細胞多維成形裝置，其中該多軸成型模組包含一列印注料頭，該列印注料頭與該出料管道連接，用以輸入該混合自體細胞並列印該人工植體。
  6. 如申請專利範圍第5項所述之自體細胞多維成形裝置，其中該多軸成型模組為一六軸成型模組，該六軸成型模組包含一圖型接收元件及一六軸移動手臂，該圖型接收元件用以接收一客製化圖案，以及該六軸移動手臂以電連接於該圖型接收元件，用以帶動該列印注料頭之一客製化列印行程。
  7. 如申請專利範圍第6項所述之自體細胞多維成形裝置，其中該圖型接收元件可利用藍芽、乙太區域網路、WiFi無線區域網路、無線行動通訊模組或其他可存取之移動式儲存設備接收該客製化圖案。
  8. 如申請專利範圍第6項所述之自體細胞多維成形裝置，其中該六軸移動手臂之移動方式為三維空間加上角度移動。
  9. 如申請專利範圍第1項所述之自體細胞多維成形裝置，其中該植體維持模組具有一適合該人工植體之密閉儲存環境。
  10. 如申請專利範圍第9項所述之自體細胞多維成形裝置，其中該植體維持模組為一移動式裝置。
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