TWI527588B - Whitening agent - Google Patents

Description

Whitening agent

This invention relates to whitening agents. More specifically, the present invention relates to a food or drink, a cosmetic or a pharmaceutical having a whitening action, which comprises a solvent extract of the genus Fagaceae.

The phenomenon of pigmentation on the skin of the elderly, freckles and skin after sun exposure, with the increase of age, is likely to occur, increase or not easily disappear and become the trouble of middle and high age groups. The melanin, which is the cause of coloring the skin, is produced by melanomasomes in the melanocytes between the epidermis and the dermis, and the produced melanin diffuses into adjacent cells by osmosis. The biochemical reaction in the black cells is presumed as follows. That is, the process in which the tyrosine acid which is an essential amino acid is dopaquinone by the action of tyrosine kinase, and then becomes a black melanin by the enzyme or non-enzymatic oxidation via red pigment and no pigment is melanin. The process of production. Therefore, the action of the tyrosinase which inhibits the first stage of the reaction is extremely important in inhibiting melanin production. The development of a whitening agent that prevents the production and precipitation of this melanin has been highly anticipated, and so far, many pharmaceuticals have been developed.

Among the drugs, ellagic acid (refer to Patent Document 1), ascorbic acid, hydroquinone, citric acid, placenta extract, and arbutin are mentioned. In addition, the plant extracting component is also an extract of Pyracantha fortuneana (refer to Patent Document 2), an extract of almond or medlar (refer to Patent Document 3), eucalyptus or jasmine (refer to Patent Document 4), or red onion. (Eleutherine plicata) (refer to Patent Document 5) and the like.

Patent Document 1: JP-A-64-79103

Patent Document 2: JP-A-2001-139482

Patent Document 3: Japanese Patent Publication No. 8-119848

Patent Document 4: Japanese Patent Publication No. 11-60467

Patent Document 5: Japanese Patent Publication No. 9-25214

However, the conventional melanin production inhibitors are unsatisfactory in terms of stability, side effects, efficacy, etc., and are also unsatisfactory in terms of supply stability or medication compliance. Therefore, the object of the present invention is to provide a whitening agent which is naturally satisfactory, has high safety to the human body, and has no problem in supply stability and is satisfactory in medication compliance.

As a result of intensive research to solve the above problems, the inventors of the present invention have found that extracts obtained by using specific solvents from specific plants have been found to have potent tyrosinase activity inhibition or melanin synthesis inhibition, and are used as whitening compositions. Things are very useful. In other words, the inventors of the present invention extract an extract obtained from an aqueous solution of a lower alcohol such as ethanol from an eucalyptus (oak type; also known as an oak) to provide a whitening composition. The review results have completed the present invention.

In other words, the invention relates to:

(1) A whitening agent comprising a solvent extract of the genus Fagaceae.

(2) A whitening agent according to (1), wherein the extract is an extract, a concentrate of the extract or a dried product of the extract.

(3) A whitening agent according to (1) or (2), wherein the genus Eucalyptus is a eucalyptus.

(4) The whitening agent according to any one of items (1) to (3), wherein the genus Fagaceae is a wood piece composed of eucalyptus wood, or a casks of whiskey or brandy aging. By.

(5) The whitening agent according to any one of (1) to (4) wherein the solvent is an aqueous solution containing a lower alcohol.

(6) A whitening agent according to (5), wherein the lower alcohol is ethanol.

(7) A whitening agent according to (6), wherein the solvent has an ethanol concentration of 10 to 100% by volume.

(8) A whitening agent according to item (6) or (7), wherein the solvent is obtained by distilling an ethanol-containing substance.

(9) The whitening agent according to (1) to (8), wherein the solvent extract is obtained by contacting the obtained ethanol-containing material with an oak barrel in an oak barrel.

(10) A whitening agent according to item (9), wherein the contact period is more than half a year.

(11) A whitening agent according to (1), which is any one of whiskey, brandy, or a concentrate or a dried product thereof.

(12) The whitening agent according to any one of items (1) to (11), which is a food or drink, a cosmetic or a pharmaceutical.

(13) The whitening agent of item (12) is a topical cosmetic or topical pharmaceutical.

(14) The whitening agent according to item (12), which is a food or beverage, an oral cosmetic or an oral pharmaceutical.

(15) An additive or formulation for use in a food or beverage, a cosmetic or a pharmaceutical product, which comprises a solvent extract of the genus Fagaceae.

(16) A melanin production inhibitor characterized by comprising a solvent extract of the genus Fagaceae.

(17) Use of a solvent extract of the genus Fagaceae to produce a whitening agent.

The solvent extract of the genus Fagaceae according to the present invention has strong whitening effect and high safety, and therefore, the solvent extract or the composition containing the solvent extract can be used for the manufacture of a whitening agent (for example, having a whitening effect) Food, cosmetics, pharmaceuticals, etc., or additives for food, cosmetics, or pharmaceuticals, etc.). Further, the genus Fagaceae or its solvent extract can be used as a raw material for the manufacture of foods, cosmetics, cosmetics or pharmaceuticals having a whitening effect.

Best mode for carrying out the invention

DETAILED DESCRIPTION OF THE INVENTION The present invention is directed to a whitening agent containing a solvent extract of the genus Fagaceae.

Examples of the Fagaceae plant of the genus Quercus sp. used in the present invention include, for example, Quercus serrata Thunb., Quercus grosseserrata Blume, Quercus crispula Blume, Quercus mongolica Fisch. var. grosseserrata. (Blume) Rehd.et Wils.), 栎 (Quercus acutissima Carruth.), 栎 (Quercus variabilis Blume), 槲 (Quercus dentata Thunb.), Quercus aliena blume, Quercus phillyraeoides A.Gray, Quercus myrsinaefolia Blume, Cyclobalanopsis myrsinaefolia (Blume) Oerst., Quercus glauca Thunb., Cyclobalanopsis glauca (Thunb .) Oerst.), Quercus paucidentata Franch., Quercus salicina Blume, Cyclobalanopsis paucidentata Kudo et Masam., Quercus sessilifolia Blume, Cyclobalanopsis sessilifolia Blume, Quercus stenophylla Makino, Quercus salicina Blume, Cyclobalanopsis salicina ( Blume) Oerst.), Quercus glauca Thunb.var.fasciata Blume, Quercus glauca Thunb.var.lacera Matsum., Quercus acuta Thunb., Cyclobalanopsis acuta (Thunb.) Oerst. ), Quercus gilva Blume, Cyclobalanopsis gilva (Blume) Oerst., Quercus alba L., Quercus arizonica, Quercus bicolor Willd., Italy (Quercus cerris L.), Quercus chrysolepis Liebm., Quercus marilandica, Quercus cornea Lour., Lyth Ocarpus cornea (Lour.) Rehd.), Quercus gambelii Nutt., Quercus agrifolia Nee, Quercus douglassii, Quercus nigra, Quercus palmeri, needle Quercus palustris, Quercus dumosa, Quercus emoryi Torr. Quercus engelmannii Greene, Quercus garryana Dougl, Quercus lobata Nee, Quercus velutina, Quercus kelloggii, Quercus macrocarpa , Quercus undulata Torr., Quercus lucombeana Sweet (Quercus suber L.), Quercus ilex L., Quercus miyagii Koidz., Cyclobalanopsis miyagii (Koidz.) Kudo Et Masamune), Quercus pungens, Quercus mongolica Fisch.var.mongolica, Quercus prinus L., Quercus robur L., Quercus falcata, Northern blood Quercus rubra, Quercus virginiana, Quercus wislizeni, Quercus stellata, Quercus semicarpifolia Sm., Quercus peraea (Mattuschka) Lieblein) and so on. Most of the plants that have been used as raw materials for the manufacture and storage of casks such as whiskey or brandy belong to this genus. In particular, plant species known as oysters (oaks) are preferred. The eucalyptus which is referred to in the present invention is a plant group used for the manufacture and storage of the casks of whiskey or brandy in the genus Fagaceae. The eucalyptus can be utilized well in the present invention. Among them, white peony, English cockroach, stalkless scorpion, Spanish scorpion, big fruit scorpion or leeches are the best.

In the above-mentioned plants, the site to be used as a raw material is not particularly limited, and trunks, leaves, branches, bark, flowers, fruits, and the like are all usable, but it is preferable to extract them from the heartwood from which the bark and the main branches are removed. Also, the extraction can be taken Take it immediately after taking it, or after drying. If necessary, it can be processed after being crushed, cut, shredded, formed, etc. Slices, wood flour, wooden barrels, etc. made from the wood of the above plants belong to the above-mentioned processed products. It is preferred to heat the inner wall with fire before the solvent extraction.

The extraction solvent used in the present invention is preferably an aqueous solution of a lower alcohol. The above lower alcohols are alcohols having 1 to 4 carbon atoms (e.g., methanol, ethanol, propanol, butanol, etc.). The concentration of the lower alcohol in the aqueous solution is preferably an extract capable of extracting a whitening effect, in particular, the concentration of the lower alcohol in the aqueous solution of the lower alcohol is usually in the range of about 10 to 100% by volume, wherein about 30 to 99% by volume is preferred. In consideration of the final formulation in foods and drinks, etc., it is preferred to use an aqueous ethanol solution from the viewpoint of safety. Further, the extraction solvent may contain other components in addition to the lower alcohol and water, without significantly impairing the extraction efficiency. For example, a water-soluble component such as a saccharide, a salt or an amino acid or various other solvents (for example, ethyl acetate, acetone, etc.) may be contained as necessary.

Therefore, when, for example, ethanol is used as the lower alcohol, the aqueous ethanol solution may be a mixture of industrial grade and water, or various alcoholic products or semi-finished products thereof. For example, brandy, whiskey, white wine, Japanese sake, beer, sparkling wine, distilled liquor, vodka or its semi-finished products. The manufacturing method can be carried out in accordance with a conventional method. When a plant material is used to form a wooden barrel and a solvent is injected therein for extraction, it is preferred that the solvent be obtained by using a distilled ethanol-containing material. The above-mentioned "distilled ethanol-containing material" means a distillate obtained by distilling an ethanol-containing solution. Specifically, malt, rice, grapes, and the like are partially saccharified as a raw material, and the ethanol-containing material obtained by fermentation is subjected to single distillation or duplex. Produced by distillation. For example, using shochu, vodka, whisky, pre-storage (such as new pot of malt whisky, new make of cereal whiskey, and fresh wine before brandy (nouvelle) good. It is better to use the original wine before the storage of whiskey and the original wine before the storage of brandy. The manufacturing method can be carried out in accordance with a conventional method. At this time, the extraction conditions are preferably carried out at room temperature for about half a year to about 30 years.

The solvent extraction method as the raw material plant in the present invention is not particularly limited, and the solvent is brought into contact with the above plant material. The raw material is immersed in a solvent, or a plant material is used to form a container such as a tub, and a solvent is also injected. Then, it is left to stand still, or heated to reflux or immersion extraction, etc., and the extraction method is appropriately set according to a desired property in accordance with a known method. The extraction can be carried out at room temperature or under heating. The extraction temperature is not particularly limited, but it is preferably at a temperature below the boiling point of the solvent. The time required for the extraction varies depending on the temperature conditions or the extraction method, and is usually about 30 minutes or more. There is no special limit to the upper limit of the extraction time, and most cases are sufficient for about 30 years. Of course, the invention also includes more than 30 years.

According to the present invention, after the above extraction, the plant of the genus Fagaceae, the processed product thereof or the processed product thereof is separated from the extract containing the whitening component by a well-known method. This separation method can be carried out in accordance with a known method such as centrifugation, filtration or the like. In the present invention, the extract may be used as a whitening agent, or the concentrate or dried product (concentrated dry matter) of the extract may be used as a whitening agent. This concentration can be carried out under normal pressure or reduced pressure. The volume of the concentrate is reduced by concentration to about 5 to 70% by volume, preferably from about 10 to 50% by volume. Dry solids can be evaporated from the extract containing whitening ingredients under reduced pressure Made with a solvent.

Further, the solvent extract of the present invention also includes an extract obtained by extraction and separation and purification by, for example, column chromatography. The solvent extract thus obtained can be used in the state of extracting the extract obtained by the extraction operation, partially removing the solvent of the extract, or completely removing the dry solid of the solvent. From the standpoint of preserving stability or being easy to handle, it is preferred to use a dry product. The solvent extract referred to in the present invention means the above-mentioned extract, concentrate or dried matter. Further, a whitening composition containing the solvent extract of the present invention is prepared, and the composition containing the extract having the whitening effect can be directly used as a whitening agent, and for example, titanium oxide, calcium carbonate, or distilled water can be added. The use of a suitable liquid or solid excipient or extender, such as lactose, starch or the specific examples described in the following examples, is used as a whitening agent. The mixing ratio of the solvent extract in the whitening agent is not limited, and may be appropriately set, for example, in the range of about 0.01 to 100% by weight, depending on the properties of the extract (for example, an extract, a concentrate or a dried product).

Further, according to the present invention, foods and drinks, cosmetics, and pharmaceuticals containing a solvent extract of the genus Fagaceae can be adjusted. The proportion of the solvent extract is not limited, and depending on the properties of the extract (for example, extract, concentrated or dried), for example, it can be adjusted in the range of about 0.01 to 100% by weight, wherein the concentration is set in the range of about 0.1 to 80% by weight. It is better.

The above foods and drinks, for example, in leeches, ingots, chewing gum, Yakult, ice cream, pudding, jelly, water alpaca, alcoholic beverages, coffee drinks, pure juices, juice drinks, carbonated drinks, refreshing drinks, milk, lotion drinks, lactic acid bacteria drinks, etc. It contains an appropriate amount of solvent extract and is provided as a food or drink. The food or drink is manufactured by mixing various additives in accordance with a conventional method as necessary.

When preparing the food or drink, for example, the following additives generally used as food materials, such as glucose, fructose, sucrose, maltose, sorbitol, stevioside, rubusoside, corn syrup, lactose, mash Acid, tartaric acid, malic acid, succinic acid, lactic acid, L-ascorbic acid, dl-α-tocopherol, sodium soda erythorbate, glycerin, propylene glycol, glycerin fatty acid ester, polyglycerin fatty acid ester, fatty acid sucrose ester, Sorbitan fatty acid ester, fatty acid propylene glycol ester, gum arabic, carrageenan, casein, gelatin, pectin, broccoli (agar), vitamin B, nicotinamide, calcium pantothenate, amino acid, calcium Salts, pigments, flavors, preservatives, etc. are manufactured according to the appropriate amount of the conventional method.

The so-called fragrance product in the present invention includes a product called a cosmetic or perfume product, and when the solvent extract of the present invention is provided, it can be used in a lotion, a cosmetic cream, an emulsion, a foundation, a lipstick, a hair styling, a hair spray, and a hair growth. Non-oral cosmetic products such as agents, shampoos, moisturizers, and body washes, or oral cosmetic products such as toothpastes, mouthwashes, mouthwashes, and oral fragrances, which contain a proper amount of solvent extract to make a fragrance. Makeup.

The fragrance can be appropriately blended with the following additives generally used as raw materials for cosmetics, such as oils such as vegetable oils, waxes such as lanolin or beeswax, hydrocarbons, fatty acids, higher alcohols, esters, various surfactants, Pigments, spices, vitamins, plants, animal extracts, UV absorbers, antioxidants, antiseptics, fungicides, moisturizers (such as urea, hyaluronic acid), etc., are manufactured according to common methods.

When used as a medicine, various additives may be added as necessary, and A pharmaceutical preparation of various dosage forms is prepared with an appropriate amount of solvent extract. For example, as a tablet, a capsule, a granule, a powder, a syrup, an extract, or the like, or an ointment, an ophthalmic ointment, a detergent, a cream, a patch, a suppository, an eye drop, It is provided as a non-oral medicine such as an injection. The pharmaceutical product can be produced according to a conventional method using various additives. The additive to be added is not particularly limited, and a general user such as a solid carrier such as starch, lactose, white sugar, mannitol, carboxymethylcellulose, corn starch, inorganic salt, distilled water, physiological saline solution, aqueous glucose solution, or ethanol can be used. Such as alcohols, liquid carriers such as propylene glycol and polyethylene glycol, various animal and vegetable oils, oily carriers such as white petrolatum, paraffin wax, and wax.

When using an aqueous solution of ethanol as a solvent or using an extract of eucalyptus, which is rich in dietary experience, as a raw material plant, it is suitable for use as a food or drink, oral medicine, oral cosmetic, etc. because it is excellent in safety when used orally. . Therefore, whiskey or brandy, or a concentrate thereof and a dried product can be used as a solvent extract to prepare a suitable food or drink, oral medicine, or oral cosmetic.

Moreover, when using the solvent extract of the present invention to prepare a food or drink, a cosmetic or a pharmaceutical, other whitening agents or melanin production inhibitors such as ellagic acid, ascorbic acid, hydroquinone, citric acid, placental extract, and the like may be used in combination. Arbutin, or extracts of plants such as Pyracantha, almond, papaya, cypress, jasmine, and red onion. The blending ratio of the plant extract component and the solvent extract of the present invention cannot be generalized, and is usually in the range of about 1:9 to 9:1 by weight.

In the present invention, the use of foods, cosmetics, cosmetics, or pharmaceuticals is added. The agent or the formulation agent refers to a solvent, a coloring matter, an antioxidant, a food or beverage, a cosmetic or a commonly used additive or a formulation in a pharmaceutical product, and a solvent extract is mixed. The mixing ratio can be appropriately set. In general, the mixing ratio thereof is, for example, in the range of about 0.01 to 100% by weight, preferably 0.1 to 80% by weight. The foods, cosmetics, or pharmaceutical additives or formulation used herein are as various additives as described above.

Example

The invention is explained in more detail below on the basis of examples. However, the invention is not limited in scope.

(Test method) Method for evaluating tyrosinase inhibitory activity

In the following examples, tyrosinase inhibitory activity was evaluated as an indicator of whitening action. The test method is as follows.

B16 murine melanoma cells were cultured in DMEM medium containing 10% fetal bovine serum at 5% carbon dioxide and 37 °C. The test substance was provided in the test by appropriately dissolving the freeze-dried product in DMSO. The test substance was mixed with a tyrosinase crude enzyme solution prepared from B16 melanoma cells, and L-dopa as a substrate was added to have a final concentration of 0.05%. The reaction was carried out at 37 ° C for 20 minutes, and the absorbance A at 492 nm was measured. The absorbance A is proportional to the amount of dopa pigment. With respect to the control group, the absorbance B at 492 nm was measured in the same manner as in the above reaction system without adding the test sample, and the inhibition ratio of tyrosine activity was calculated according to the following formula.

Inhibition rate (%) = (1-A/B) × 100

The concentration of the test sample (μg/reaction solution ml) was changed in a gradient manner, and the above-described inhibition rate was measured, and the concentration of the sample at an inhibition rate of 50% was determined by interpolation, and this concentration was taken as the IC ₅₀ .

Example 1

The effect of whitening extract on the whitening effect was investigated.

(Invention product 1):

Industrial ethanol was mixed with water to obtain a 60% by volume aqueous solution of ethanol. White eucalyptus tablets (1 × 1 × 2 cm) (240 g) were added to the aqueous ethanol solution (2.4 L), and the mixture was heated at 85 ° C for 5 minutes, left at room temperature for 24 hours, and further heated at 85 ° C for 5 minutes. The obtained extract was dried under reduced pressure to give a dried product (7.2 g).

(Invention products 2 to 5):

Prepare a new pot for the original sake of the whisky. That is, the germinated barley (malt) is pulverized, mixed with warm water to be saccharified, and yeast is added to the filtered sugar liquid to ferment it, and the alcohol content is 7.0 to 7.5% by volume. The crucible was placed in a copper single distiller and distilled twice to obtain an alcoholic composition (i.e., a new jug) having an alcohol concentration of 60% by volume.

Secondly, the use of white oysters to make whiskeys for the manufacture of whisky (content is 230L). The inner wall of the barrel is heated by direct fire.

210L of the above alcohol solvent (ie, new pot) was placed in the wine barrel, and after embolization, it was stored in a storage room at room temperature. The extract was taken over time at the time of 3 years (invention product 2), 5 years (invention product 3), 12 years (invention product 4), and 18 years (invention product 5). The obtained extract was dried under reduced pressure as a substance to be inspected.

(Invention product 6):

Extracts that are manufactured in the same way and have a shelf life of more than 12 years A plurality of blended Santori whiskies Yamazaki (registered trade name) were dried under reduced pressure for 12 years to obtain a test substance (invention product 6).

The tyrosinase inhibitory activity of the inventive article was measured according to the above method.

Regarding the control, a new pot was dried under reduced pressure (Comparative item 1). The results are shown in Table 1.

The product of the present invention was confirmed to have potent tyrosinase inhibitory activity compared to the control. In particular, when a new pot is used as a solvent, the longer the storage period, the stronger the effect.

The article of the present invention showed a potent effect equal to or higher than that of ellagic acid as compared with the results of the same test system evaluation (IC ₅₀ = 137 μg/ml) used in various cosmetic or topical pharmaceutical products.

It can be seen from the above that the product of the present invention has a strong whitening effect, and in particular, the longer the storage period, the stronger the whitening effect. These preparations are prepared by a method in which an aqueous solution of ethanol is used as a solvent and is prepared according to the experience of whisky, and a whitening agent having a high safety for oral administration can be provided.

Example 2

To explore the effect of British cockroach extract on the whitening effect.

(Invention product 7):

Industrial ethanol and water were mixed to form a 70% by volume aqueous solution of ethanol. In the aqueous ethanol solution (10 L), 1 kg of Limouson, one of the British cockroaches, was finely cut, heated at 85 ° C for 5 minutes, left at room temperature for 24 hours, and then heated at 85 ° C for 5 minutes. . The obtained extract was dried under reduced pressure to give 148 g of dried material.

(Invention products 8 to 11):

The modulation is used as a nouvelle for the original wine before the brandy is stored. That is, the white grape is crushed and the juice is extracted, and the yeast is added to ferment it, and a grape fermentation liquid having an alcohol concentration of 8 to 10% by volume is obtained. Distilled in a distiller to contain wine An alcoholic composition (nouvelle) having a concentration of 70% by volume.

Next, a barrel made of brandy was produced using British 栎 (content amount: 370 L). The inner wall of the barrel is heated by direct fire.

Next, 350 L of the above ethanol solvent (i.e., nouvelle) was placed in the barrel for the manufacture of brandy, plugged, and stored in a storage room at room temperature. It is taken at the time of 7 years (invention product 8), 10 years (invention product 9), 15 years (invention product 10), 20 years (invention product 11), and the obtained extract is dried under reduced pressure as a Test the substance.

(Invention Product 12):

The extracts obtained by long-term storage in the same manner are mixed and the "Sandoli Brandy Best" (registered trademark) is dried under reduced pressure to obtain a test substance (invention product 12).

The tyrosinase inhibitory activity of each of the inventive articles was measured in accordance with the above method.

Regarding the control, the nouvelle was dried under reduced pressure (Control 2). The results are shown in Table 2.

The articles of the present invention were confirmed to have potent tyrosinase inhibitory activity compared to the control. In particular, when nouvelle is used as a solvent, the longer the storage period, the stronger the effect.

The articles of the present invention were confirmed to have potent tyrosinase inhibitory activity compared to the control. In particular, when nouvelle is used as a solvent, the longer the storage period, the stronger the effect.

From the above, it is shown that the whitening effect of the product of the present invention is strong, and in particular, the longer the preservation period, the stronger the whitening effect. These products are prepared by using an aqueous solution of ethanol as a solvent and a method of producing brandy which is rich in dietary experience, so that a whitening agent having a high safety for oral administration can be provided.

Example 3

Inhibition of melanin production in the cells was evaluated using the inventive article 6.

The above-mentioned inventive product 6 (i.e., Santo Dolly Yamazaki (registered trademark) 12 years) was used for the substance to be inspected. Specifically, Santori whiskey Yamazaki (registered trademark) was dried under reduced pressure for 12 years, and used at a final concentration of 100 μg/ml.

The amount of melanin in the cells was measured by the following method, and 4 × 10 ⁴ B16 melanoma cells were preliminarily placed in a 60 mm plastic petri dish. The test substance was added after 24 hours of preculture. After culturing the cells for 3 days in the presence of the test substance, the cells were washed with PBS, and 1 N sodium hydroxide was added thereto and dissolved by heating. The absorbance C at 470 nm of the culture solution was measured. The absorbance C is proportional to the amount of melanin. Regarding the reference product, the same operation was carried out in the above reaction system without adding the test sample, and the absorbance D at 470 nm was measured, and the intracellular melanin production inhibition rate was calculated by the following formula.

Inhibition rate (%) = (1-C/D) × 100

In addition, the number of cells was measured at the same time to investigate the effect of the sample on cell proliferation. The results are shown in Table 3.

The inventive article 6 showed a decrease in intracellular melanin production. Further, the cell proliferation of the product of the present invention is the same as that of the control, and the inventive article has no effect on cell proliferation.

From the above, it is understood that the composition of the present invention can significantly reduce the amount of melanin production in cells and provide a highly safe whitening agent.

Example 4

A pharmaceutical product containing the solvent extract of the present invention was produced in the following manner.

Tablets: 1 kg of white oak chips were placed in a stainless steel container, and an aqueous solution (30 L) having an ethanol concentration of 60% by volume was added, and the mixture was stirred at 60 ° C for 3 hours. The solvent was removed from the obtained extract to obtain 15 g of a white peony extract (dry product).

The dried product (5 g) was mixed with lactose (490 g) and magnesium stearate (5 g) to form a tablet having a diameter of 10 mm and a weight of 300 mg in a single-piece tableting mechanism.

Granules: The above-mentioned tablets are pulverized, and granules of 20 to 50 mesh are obtained by sieving.

Example 5

The white peony extract was prepared in the same manner as in Example 4, and various foods and drinks containing the solvent extract of the present invention were produced in the following composition. The chalk extract (dry product) in the following composition was prepared in Example 4.

Otter:

candy:

Ingots:

chewing gum:

Milk sugar:

Jelly (coffee jelly):

ice cream:

Kstar pudding:

Water sheep:

fruit juice:

Carbonated drinks:

Lactic acid bacteria drink:

Coffee drink:

Alcoholic beverages:

Example 6

The white peony extract was prepared in the same manner as in Example 4, as shown below. It is composed of various cosmetic products containing the solvent extract of the present invention. The extract of the white peony (dried product) in the following composition was obtained in Example 4.

Tooth powder:

Washing liquid:

Soft makeup (weak acidity):

Moisturizer:

Moisturizer:

Emulsion foundation: Trace of spices

Hair water:

Shampoo:

Hair conditioner:

Industrial use

The present invention relates to a food or drink, a cosmetic or a pharmaceutical having a whitening action, which comprises a solvent extract of the genus Fagaceae.

Claims (10)

A whitening agent characterized by containing a solvent extract of white peony or British cockroach belonging to the genus Fagaceae, and being any one of whiskey or a concentrate or a dried product thereof, the solvent is 60 to 70% by volume The aqueous solution of ethanol is used as a raw material of the genus Fagaceae, and the heart material of the bark is removed from the trunk and the main branch; however, the case where the heartwood is in the form of a wood chip is excluded. The whitening agent of claim 1, wherein the extract is an extract, a concentrate of the extract or a dried extract. For example, in the whitening agent of the first aspect of the patent application, the genus Fagaceae is in the form of a casks for the formation of whiskey composed of eucalyptus wood. The whitening agent of claim 1, wherein the solvent is obtained by distilling ethanol-containing material. The whitening agent of claim 4, wherein the solvent extract is obtained by contacting the obtained ethanol-containing material with oak barrels in oak barrels. For example, the whitening agent of claim 5, wherein the contact period is more than half a year. The whitening agent according to any one of claims 1 to 6 is a food or drink, a cosmetic or a pharmaceutical. For example, the whitening agent of claim 7 is a topical cosmetic or topical pharmaceutical. For example, the whitening agent of claim 7 is a food or beverage, an oral cosmetic or an oral pharmaceutical. The use of a solvent extract of a heart material of a bark and a scorpion of the genus Fagaceae, which removes the bark from the trunk and the main branch, is used for the manufacture of a whitening agent, wherein the solvent is 60 to 70% by volume of ethanol An aqueous solution; however, the case where the shape of the heartwood is excluded from wood chips.