KR20230099245A - Composition For Improving Skin Anti-aging Or Promoting Skin Regeneration Comprising Fermented Red Ginseng Sugared Solution - Google Patents

Abstract

The present invention provides a composition for improving skin anti-aging or promoting skin regeneration, comprising fermented red ginseng sugar syrup obtained by inoculating and fermenting Saccharomyces strain in red ginseng sugar syrup. The fermented red ginseng sugar syrup solution of the present invention may exhibit anti-aging effects such as wrinkle improvement and elasticity enhancement, or skin regeneration promoting effects by increasing skin collagen synthesis.

Description

Composition for Improving Skin Anti-aging Or Promoting Skin Regeneration Comprising Fermented Red Ginseng Sugared Solution}

The present invention relates to a composition for improving skin anti-aging or promoting skin regeneration, comprising fermented red ginseng sugar syrup.

As the skin ages, the aging process intensifies. exposed to sunlight for a long period of time When exposed, changes occur in the epidermal and dermal tissues, resulting in increased wrinkles, thickening of the skin, loss of elasticity, and roughening to form spots. Since such skin aging can be delayed by promoting the synthesis of collagen, research into developing materials that are safe for the living body while promoting the synthesis of collagen is being actively conducted.

Collagen is a major component of the extracellular matrix and is the major matrix protein produced by fibroblasts in the skin. In addition, it is an important protein that accounts for about 30% of the total weight of biological proteins and has a solid triple helix structure. Collagen forms most of the organic matter of skin, tendons, bones and teeth, and its content is particularly high in bones and skin. It is present as fibrous inclusions in most other body structures.

It is known that the main functions of collagen include mechanical robustness of the skin, resistance and cohesiveness of connective tissue, support of cell adhesion, induction of cell division and differentiation (in the growth of organisms or wound healing), and the like. In recent years, as extensive research on skin aging has been conducted, the important function of collagen in the skin has been revealed. For example, it is known that collagen is reduced by age and photoaging by ultraviolet irradiation, which is closely related to wrinkle formation of the skin. In addition, collagen plays an important role in wound healing, and by promoting the synthesis of collagen in damaged epithelium, wounds can be recovered quickly and without scarring. As described above, when collagen metabolism is activated by promoting collagen synthesis, the components of the dermal matrix are increased, and thus the effects of wrinkle improvement, elasticity enhancement, skin strengthening, and wound healing can be expected.

In order to obtain this effect, conventionally, products in which collagen is mixed with cosmetics have been released to use the skin wrinkle improvement effect of collagen. Since an improvement effect could not be expected, it could not be said to be an essential skin function improvement.

Therefore, interest in substances that can promote collagen synthesis in the skin itself has increased, and conventional substances that can promote collagen synthesis include retinoic acid, TGF (transforming growth factor), chlorella extract, and the like. It is known (Patent Document 1). However, retinoic acid is unstable and there is a limit to the amount used due to stability problems such as irritation and redness when applied to the skin, and chlorella extract and the like are insignificant, so the effect of improving skin function by promoting collagen synthesis in the skin cannot be expected. Therefore, studies on collagen synthesis promoting components that are safe for the living body while exhibiting effects similar to or better than existing collagen synthesis promoting substances have been continuously conducted.

The present inventors confirmed that the fermentation product of red ginseng syrup obtained by inoculating and fermenting Saccharomyces cerevisiae strain in red ginseng syrup obtained by removing red ginseng after soaking red ginseng was excellent in increasing skin collagen synthesis. and completed the present invention.

Republic of Korea Patent Publication No. 2004-0026148

An object of the present invention is to provide a composition containing natural ingredients that can exhibit effects such as wrinkle improvement, elasticity enhancement, and skin regeneration promotion by increasing collagen synthesis in the skin.

One aspect of the present invention provides a composition for improving skin anti-aging comprising a fermented red ginseng sugar syrup obtained by inoculating and fermenting a Saccharomyces strain in red ginseng sugar syrup.

Another aspect of the present invention provides a composition for promoting skin regeneration comprising a fermented red ginseng sugar syrup obtained by inoculating and fermenting a Saccharomyces strain in red ginseng sugar syrup.

The fermented red ginseng sugar syrup solution of the present invention may exhibit anti-aging effects such as wrinkle improvement and elasticity enhancement, or skin regeneration promoting effects by increasing skin collagen synthesis.

1 is a result showing the collagen synthesis enhancing effect of the fermented red ginseng sugar syrup according to the present invention.

Hereinafter, the present invention will be described in more detail to aid understanding of the present invention.

Terms or words used in this specification and claims should not be construed as being limited to ordinary or dictionary meanings, and the inventor may appropriately define the concept of terms in order to explain his or her invention in the best way. It should be interpreted as a meaning and concept consistent with the technical idea of the present invention based on the principle that there is.

1. Preparation of fermented red ginseng sugar syrup

In order to obtain fermented red ginseng sugar syrup, red ginseng sugar syrup is inoculated with and fermented with Saccharomyces strain.

In the present invention, red ginseng sugar syrup may be used in a broad sense to refer to sugar syrup containing red ginseng-derived ingredients, and the manufacturing method may be conventional.

In the present invention, red ginseng is steamed, cooked, and dried raw ginseng, and a browning reaction occurs during the drying process, resulting in a light yellowish brown to reddish brown color. Ginseng can be used without limitation, such as cultivated or commercially available. The ginseng includes variously processed ones, and may be, for example, at least one selected from the group consisting of fresh ginseng, misam, wild ginseng, camphor ginseng, wild ginseng, dextrose ginseng, enzyme-treated misam, and fermented ginseng, and is limited thereto. It doesn't work. Fresh ginseng refers to raw ginseng that has maintained its original form, and misam refers to the thin root of ginseng. Wild ginseng refers to ginseng that grows naturally, and camphor ginseng refers to wild ginseng that has been planted and grown. Sanyangsam is ginseng cultivated by artificially planting and transplanting seeds or seedlings under forests in the mountains.

In the present invention, red ginseng may be used as a raw material, but if necessary, red ginseng powder cut into a certain size that is easy to scan or red ginseng powder may be used. In the case of using cut red ginseng, it may be cut to a thickness of 2 to 3 mm, and in the case of red ginseng powder, it may be used in the sense of including both red ginseng powder or red ginseng concentrate powder, but preferably means red ginseng ground powder. .

For example, in order to obtain a red ginseng sugar acupuncture solution, sugar acupuncture can be performed by adding red ginseng to sugar solution and then immersing it under certain conditions. Specifically, the needle acupuncture may be performed at 25 to 85°C, 45 to 75°C, or 65 to 70°C for 1 to 24 hours, 3 to 12 hours, or 5 to 8 hours.

The red ginseng may be added in an amount of 1 to 20 parts by weight, 5 to 15 parts by weight, or 10 to 12 parts by weight based on 100 parts by weight of the sugar solution.

The sugar solution is sugar, honey, liquid fructose, starch syrup, high-intensity sweetener, maple syrup, agave syrup, fructooligosaccharide, galactooligosaccharide, xylo-oligosaccharide, lacto-oligosaccharide, maltooligosaccharide, chitooligosaccharide, isomaltooligosaccharide, soybean oligosaccharide, At least one selected from the group consisting of raffinose, maltitol, and mannitol may be included, and honey is preferably used. Red ginseng syrup obtained by using honey as a sugar solution may also be referred to as red ginseng honey extract.

In the present invention, since the red ginseng needle saliva remaining after removing the red ginseng needle is not used, such as red ginseng slices obtained by needle acupuncture, red ginseng is removed to obtain the red ginseng needle needle. A method for removing red ginseng is not particularly limited, and a conventional method such as filtration may be used.

In the present invention, the Saccharomyces strain may be a Saccharomyces cerevisiae strain. Since Saccharomyces cerevisiae strains play a role in converting glucose into alcohol, they are used to manufacture various alcoholic beverages such as wine and beer, and are also used to make fermented bread. Although Saccharomyces cerevisiae strains have various subtypes and have different properties accordingly, the present invention is not particularly limited thereto.

Inoculation of the strain may be performed by adding 0.1 to 1% of the Saccharomyces strain compared to the red ginseng sugar solution.

The fermentation may be carried out at 30 to 47 ° C, or 35 to 45 ° C, or 35 to 38 ° C for 24 to 216 hours, or 48 to 192 hours, or 72 to 168 hours. When the above conditions are satisfied, the anti-aging effect or skin regeneration promoting effect of the obtained fermented red ginseng sugar syrup may be the best.

As described above, in the present invention, the fermented red ginseng sugar syrup refers to a product obtained by inoculating and fermenting Saccharomyces strain in red ginseng sugar syrup, and the process of fermenting the sugar syrup using the Saccharomyces strain is used to produce alcoholic beverages such as wine Similar to the process, the fermented product of red ginseng sugar syrup of the present invention is also referred to as red ginseng wine, red ginseng bongmilju, red ginseng wine, or ginseng wine.

However, since the fermented red ginseng sugar syrup of the present invention utilizes the saponin or non-saponin fraction contained in the fermented red ginseng sugar syrup rather than alcohol, which is a metabolite of sugar, the sugar content is as low as 10brix or less, and the same as ginsenosides. The red ginseng component is prepared using a sugar soaking liquid that is relatively high in the level of 1 to 10% of conventional red ginseng concentrate. Therefore, there is a difference from ordinary alcohol or wine in that alcoholic fermentation does not occur smoothly when fermentation is performed with such a glucose solution.

The red ginseng syrup of the present invention may have a content of 11 ginsenosides of 3 to 25 μg/ml, or 5 to 20 μg/ml, or 7 to 15 μg/ml.

In addition, although the total content of 11 ginsenosides may not change significantly after fermentation, the composition ratio of ginsenosides may change as the content of specific ginsenoside components is reduced. For example, the fermented red ginseng sugar syrup of the present invention may have 11 ginsenosides in a content of 3 to 25 μg/ml, 5 to 20 μg/ml, or 7 to 15 μg/ml, and Rc, Rb2 And it may not contain at least one selected from the group consisting of Rd.

Until recently, since ginsenoside components are known to have excellent anti-aging improving effects, studies on the anti-aging improving effects of each ginsenoside component have been actively conducted. By the way, according to the experimental example of the present invention, despite the fact that the fermented red ginseng sugar soak solution had a lower ginsenoside content than the red ginseng concentrate, it showed a much better collagen synthesis enhancing effect than the red ginseng concentrate.

In addition, although the fermented red ginseng sugar syrup of the present invention showed a ginsenoside content similar to that of unfermented red ginseng sugar syrup, the effect of enhancing collagen synthesis was much better than that of the red ginseng sugar syrup. This is believed to further enhance the collagen synthesis effect of red ginseng components such as existing ginsenosides, as components such as polysaccharides or amino sugars capable of enhancing skin collagen synthesis are formed in the process of fermenting red ginseng sugar syrup. The amino sugar may be an arginine-fructose-glucose (AFG) amino sugar, but is not particularly limited thereto as long as it can exhibit a collagen synthesis enhancing effect.

2. Composition for improving skin anti-aging or promoting skin regeneration

One aspect of the present invention provides a composition for improving skin anti-aging comprising a fermented red ginseng sugar syrup obtained by inoculating and fermenting a Saccharomyces strain in red ginseng sugar syrup.

Another aspect of the present invention provides a composition for promoting skin regeneration comprising a fermented red ginseng sugar syrup obtained by inoculating and fermenting a Saccharomyces strain in red ginseng sugar syrup.

The composition for improving skin anti-aging or promoting skin regeneration may be a cosmetic composition.

The manufacturing method of the fermented red ginseng sugar syrup is the same as that mentioned in " 1. Preparation of the fermented red ginseng sugar syrup ", so the description is omitted.

The cosmetic composition of the present invention contains the fermented red ginseng sugar syrup in an amount of 0.0001 to 10% by weight, or 0.001 to 5% by weight, or 0.01 to 3% by weight, or 0.1 to 2% by weight, or 0.5 to 1.5% by weight, based on the total weight of the cosmetic composition. It can be included in weight percent. When the above numerical range is satisfied, as the collagen synthesis ability of the skin is further improved, the effect of improving skin anti-aging or promoting skin regeneration may be excellent.

The cosmetic composition of the present invention may be prepared in liquid or solid form using bases, adjuvants and additives commonly used in the field of cosmetics. Cosmetics in liquid or solid form may include, but are not limited to, cosmetic products, creams, lotions, and bath products.

Bases, adjuvants and additives commonly used in the field of cosmetics are not particularly limited, and examples thereof include water, alcohol, propylene glycol, stearic acid, glycerol, cetyl alcohol, and liquid paraffin.

The cosmetic composition of the present invention may include ingredients commonly used in cosmetic compositions, as well as fermented red ginseng sugar syrup, such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and flavors, and A carrier may be included.

The cosmetic composition of the present invention may be prepared in any formulation commonly prepared in the art, for example, solution, suspension, emulsion, paste, gel, cream, lotion, powder, soap, surfactant-containing cleansing , It may be formulated as an oil, powder foundation, emulsion foundation, wax foundation and spray, but is not limited thereto. More specifically, it may be prepared in the form of softening lotion, nourishing lotion, nourishing cream, massage cream, essence, eye cream, cleansing cream, cleansing foam, cleansing water, pack, spray or powder.

When the formulation of the present invention is a paste, cream or gel, animal oil, vegetable oil, wax, paraffin, starch, tracanth, cellulose derivative, polyethylene glycol, silicone, bentonite, silica, talc or zinc oxide may be used as a carrier component. can

When the formulation of the present invention is a powder or spray, lactose, talc, silica, aluminum hydroxide, calcium silicate or polyamide powder may be used as a carrier component, and in particular, in the case of a spray, additional chlorofluorohydrocarbon, propane / May contain a propellant such as butane or dimethyl ether.

When the formulation of the present invention is a solution or emulsion, a solvent, solubilizing agent or emulsifying agent is used as a carrier component, such as water, ethanol, isopropanol, ethyl carbonate, ethyl acetate, benzyl alcohol, benzyl benzoate, propylene glycol, 1 ,3-butylene glycol oil, glycerol aliphatic esters, polyethylene glycol or fatty acid esters of sorbitan.

When the formulation of the present invention is a suspension, as a carrier component, a liquid diluent such as water, ethanol or propylene glycol, a suspending agent such as ethoxylated isostearyl alcohol, polyoxyethylene sorbitol ester and polyoxyethylene sorbitan ester, microcrystals Star cellulose, aluminum metahydroxide, bentonite, agar or tracanth and the like may be used.

When the formulation of the present invention is surfactant-containing cleansing, as carrier components, aliphatic alcohol sulfate, aliphatic alcohol ether sulfate, sulfosuccinic acid monoester, imidazolinium derivative, methyl taurate, sarcosinate, fatty acid amide ether sulfate, alkyl Amidobetaine, fatty alcohols, fatty acid glycerides, fatty acid diethanolamides, vegetable oils, lanolin derivatives, or ethoxylated glycerol fatty acid esters may be used.

The cosmetic composition of the present invention may be used alone or overlappingly applied, or may be used overlappingly with other cosmetic compositions other than the present invention. In addition, the cosmetic composition with excellent skin regeneration effect according to the present invention can be used according to a conventional method of use, and the number of times of use can be varied according to the user's skin condition or taste.

When the cosmetic composition of the present invention is a soap, surfactant-containing cleansing or surfactant-free cleansing formulation, it may be applied to the skin and then wiped off, removed, or washed with water. As specific examples, the soap is liquid soap, powder soap, solid soap, and oil soap, the surfactant-containing cleansing formulation is a cleansing foam, cleansing water, cleansing towel, and a cleansing pack, and the surfactant-free cleansing formulation is a cleansing cream. , cleansing lotion, cleansing water and cleansing gel, but are not limited thereto.

2. Health functional food composition for improving skin anti-aging or promoting skin regeneration

In the present invention, the composition for improving skin anti-aging or promoting skin regeneration may be a health functional food composition.

The health functional food composition of the present invention contains the fermented red ginseng sugar syrup in an amount of 0.0001 to 10% by weight, or 0.001 to 5% by weight, or 0.01 to 3% by weight, or 0.1 to 2% by weight, based on the total weight of the health functional food composition. Or it may be included in 0.5 to 1.5% by weight. When the above numerical range is satisfied, as the collagen synthesis ability of the skin is further improved, the effect of improving skin anti-aging or promoting skin regeneration may be excellent.

The health functional food composition is the fermented product of the red ginseng sugar syrup of the present invention. In addition, within the range that does not impair the desired effect of the present invention, other components that can give a synergistic effect to the effect of the fermented red ginseng sugar soak may be additionally contained. Conventional adjuvants such as, for example, stabilizers, solubilizers, vitamins, etc., or carriers may be included.

In addition, it may include ingredients commonly added during food preparation, and include, for example, proteins, carbohydrates, fats, nutrients, seasonings, and flavoring agents. Examples of such carbohydrates include monosaccharides such as glucose, fructose, and the like; disaccharides such as maltose, sucrose, oligosaccharides and the like; and polysaccharides such as conventional sugars such as dextrins and cyclodextrins and sugar alcohols such as xylitol, sorbitol and erythritol. As the flavoring agent, natural flavoring agent thaumatin, stevia extract, and synthetic flavoring agent may be used. For example, when the health functional food composition of the present invention is prepared as a drink, citric acid, high fructose corn syrup, sugar, glucose, acetic acid, malic acid, fruit juice, natural extract, etc. may be further included in addition to the fermented red ginseng sugar syrup of the present invention.

There is no particular limitation on the type of food using the health functional food composition. Examples of foods to which the health functional food composition containing the fermented red ginseng sugar syrup of the present invention can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, chewing gum, and ice cream. Dairy products, various soups, beverages, tea, drinks, alcoholic beverages and vitamin complexes, etc., include all foods in a conventional sense.

The health functional food composition may be prepared and processed in the form of tablets, capsules, powders, granules, liquids, pills, etc. for the purpose of improving skin anti-aging or promoting skin regeneration.

For example, for food in tablet form, a mixture obtained by mixing the health functional food composition with excipients, binders, disintegrants, and other additives is granulated in a conventional manner, and then compression molding is performed by adding a lubricant or the like, or the mixture is It can be directly compression molded. In addition, the food in tablet form may contain a flavoring agent or the like as needed.

Hard capsules among food in the form of capsules can be prepared by filling a mixture obtained by mixing the health functional food composition with additives such as excipients in a normal hard capsule, and the soft capsules are prepared by mixing the health functional food composition with additives such as excipients. It can be prepared by filling a mixture into a capsule base such as gelatin. The soft capsule may contain a plasticizer such as glycerin or sorbitol, a colorant, a preservative, and the like, if necessary.

The food in the form of a pill may be prepared by molding a mixture obtained by mixing the health functional food composition with an excipient, a binder, a disintegrant, etc. by a conventionally known method, and, if necessary, may be coated with sucrose or other coating agent, or The surface can also be coated with a material such as starch or talc.

Food in the form of granules can be prepared in granular form by a conventionally known method of mixing the health functional food composition with excipients, binders, disintegrants, etc., and may contain flavoring agents, flavoring agents, etc., if necessary. .

Hereinafter, the present invention will be described in detail with reference to preparation examples and examples.

However, the following Preparation Examples and Examples are only for exemplifying the present invention, and the contents of the present invention are not limited by the following Preparation Examples and Examples.

Preparation Example 1 - Red Ginseng Concentrate

After adding 10 folds of purified water to 350 g of red ginseng roots, extraction was performed at 85 ° C for 12 hours, cooled to room temperature, foreign substances were removed, and concentrated under reduced pressure at 55 ° C until the concentrate reached 72 brix.

Preparation Example 2 - Red Ginseng Glucose Solution

After adding 900 g of honey (Honey Tea, premium miscellaneous honey) to 100 g of red ginseng slices, it was immersed at 68 ° C for 5 hours to prepare sealed slices of red ginseng, and the remaining red ginseng syrup was obtained. The glycosylation solution was concentrated at 55° C. until reaching 72 brix.

Preparation Example 3 - Fermented product of red ginseng sugar syrup

After diluting to 10 brix or less by adding sterilized water about 10 times the red ginseng syrup, Saccharomyces cerevisiae strain (Research Institute of Bioscience and Biotechnology, Center for Biological Resources, KCTC No. 7913) was added at 0.5 of the total weight of the syrup. %, and fermented at 37°C for 4 to 7 days. After completion of fermentation, sterilization was obtained, and a filtered liquid was obtained, and all moisture was removed to obtain a fermented product of red ginseng sugar soaked liquid.

Production Example 4 to Production Example 7

Instead of the red ginseng of Preparation Example 3, ginseng fruit (Korea Ginseng Corporation). The sugars of ginseng berries, soap paste, stringy bamboo shoots, and honey paste were prepared in the same way, except that fermented sugar saliva was prepared using soap paste (Rural Development Administration), stringy bamboo shoots (Rural Development Administration), and honey grass (Rural Development Administration). A soaked fermentation product was prepared.

Experimental Example 1

For the samples of Preparation Examples 1 to 3, the content (μg/ml) of 11 ginsenosides was analyzed by a conventional ginsenoside HPLC analysis method specified in the Health Functional Food Code, and the results are shown in Table 1. At this time, analysis was performed on the dilution solution of Preparation Example 2 diluted to 10 brix or less by adding about 10 times of sterile water.

Rg1 Re Rf Rh1 Rg2s Rb1 Rc Rb2 Rd Rg3s Rg3r total Red Ginseng Concentrate 22.4 26.1 15.7 12.3 16.3 101.9 41.2 37.7 16.7 23.7 10.6 324.6 glucose solution 2.07 1.08 0.88 0.71 N.D. 2.19 1.09 1.19 1.24 0.54 0.54 11.53 Fermented sugar syrup 2.55 0.86 1.36 2.23 0.93 1.47 N.D. N.D. N.D. 0.52 1.34 11.26

*N.D.: Not detected.

From the results of Table 1, it can be confirmed that the red ginseng sugar syrup and the fermented red ginseng sugar syrup have a lower ginsenoside content than the red ginseng concentrate. On the other hand, since there is not much difference in ginsenoside content in the case of fermented red ginseng sugar syrup and red ginseng sugar syrup, the total amount of 11 ginsenosides before and after fermentation using the Saccharomyces cerevisiae strain does not change significantly. there is.

Experimental Example 2

In order to confirm the preservation of the samples of Preparation Examples 1 to 3, 1% of 1,2-hexanediol was added to each sample, and then E. coli was inoculated 1*10 ⁶ and cultured in an incubator. On days 3, 7, 14, 21, and 28, viable cells were examined to evaluate the degree of microbial contamination.

0 3 7 14 21 28 Red Ginseng Concentrate 1*10 ⁶ N.D. N.D. N.D. N.D. N.D. Red ginseng sugar saliva 1*10 ⁶ TNTC TNTC TNTC TNTC TNTC Fermented red ginseng sugar solution 1*10 ⁶ 3,800 20 N.D. N.D. N.D.

*N.D.: not detected

**TNTC: Uncountable

From the results of Table 2, it can be confirmed that the fermented red ginseng sugar syrup of the present invention has a much lower degree of microbial contamination than the red ginseng sugar syrup. Therefore, since the fermented red ginseng sugar syrup of the present invention has excellent preservation properties, it can be sufficiently used in cosmetic compositions or health functional food compositions.

Experimental Example 3

Skin fibroblasts (ATCC, USA) were divided into 1×10 ⁵ each in a 24-well plate, and then DMEM:F12 mixed medium containing 10% bovine serum, and then cultured in a 37°C, 5% CO ₂ incubator. After 24 hours, each sample was treated with a concentration of 100 μg/ml, and as a positive control group, L-ascorbic acid was treated with a concentration of 100 μg/ml. After 24 hours of sample treatment, only the supernatant was separated and the increase or decrease of procollagen in the supernatant was observed using a procollagen type (I) ELISA kit (Takara, Japan).

In addition, for comparison, the mixture of the red ginseng concentrate and honey of Preparation Example 1 and the honey itself were also measured for an increase or decrease in collagen synthesis.

For each sample, the increase in procollagen type I synthesis compared to the untreated group (%) was calculated and shown in FIG.

From the results of FIG. 1 , it can be confirmed that the fermented red ginseng sugar saliva solution of the present invention has a very excellent skin collagen synthesis enhancement effect compared to red ginseng concentrate or red ginseng sugar saliva solution.

Referring to the results of Experimental Example 1 above, the fermented red ginseng sugar syrup of the present invention contains much less ginsenoside than the red ginseng concentrate, and contains ginsenosides to a similar extent to the red ginseng syrup, which has collagen synthesis ability. It is presumed that ginsenoside is not a component that mainly contributes to the promotion of collagen synthesis because it is much more increased than red ginseng concentrate or red ginseng syrup.

On the other hand, in the case of fermented ginseng berries, soapwort, stringy herb, and honeysuckle in Preparation Examples 4 to 7, the collagen synthesis effect was not shown, and in the case of the mixture of red ginseng concentrate and honey and honey itself, collagen synthesis was rather inhibited. Through this, it can be seen that honey used for sugar needle itself has no effect of enhancing collagen synthesis, and that red ginseng-derived ingredients contribute to the improvement of collagen synthesis ability in the fermentation process of red ginseng sugar needle solution of the present invention, such as polysaccharides or AFG amino sugars. It is believed to be transformed into ingredients. However, the increase in collagen biosynthesis of AFG amino sugar alone did not explain all of the collagen synthesis enhancement effects of the fermented red ginseng sugar syrup, so it seems that various other active ingredients that have not been confirmed are produced.

Experimental Example 4

After diluting the red ginseng sugar syrup and red ginseng sugar syrup of Preparation Example 2 in purified water and inoculating the Saccharomyces cerevisiae strain, each sample was measured using a saccharimeter to measure the sugar content before and after fermentation. .

As a result, the sugar content was 76.2brix in red ginseng syrup, 7.31brix before fermentation after dilution, and no sugar was detected after fermentation. From this result, it can be seen that the fermented red ginseng sugar syrup of the present invention is suitable for use in cosmetics and beverage compositions because the sugar content is reduced after fermentation of red ginseng sugar syrup.

Preparation Example 8. Preparation of cosmetic composition

8-1. Preparation of Essence

Essence was prepared using the fermented red ginseng sugar syrup of Preparation Example 3 according to the contents (parts by weight) shown in Table 3 below.

Furtherance Content (parts by weight) triethanolamine 0.25 carboxyvinyl polymer 0.22 glycerin 4 Butylene Glycol 2 Red ginseng sugar syrup fermented product of Preparation Example 3 1.5 beeswax 0.5 Cetostearyl Alcohol One Glyceryl Monostearate One squalene 4 Purified water Appropriate amount

8-2. Manufacture of softening lotion

Softening lotion containing the fermented red ginseng sugar syrup of Preparation Example 3 as an active ingredient was prepared as shown in Table 4 below.

Raw material Content (parts by weight) 1,3-butylene glycol 1.00 Disodium EDT 0.05 allantoin 0.10 dipotassium glycyrrhizate 0.05 Citric Acid 0.01 sodium citrate 0.02 glycereth-26 1.00 Arbutin 2.00 PEG-40 Hydrogenated Castor Oil 1.00 ethanol 30.00 Red ginseng sugar syrup fermented product of Preparation Example 3 0.5 coloring agent a very small amount flavoring agent a very small amount Purified water balance

8-3. Manufacture of nourishing cream

Nutritional cream containing the fermented red ginseng sugar syrup of Preparation Example 3 as an active ingredient was prepared according to the composition shown in Table 5 below.

Raw material Content (parts by weight) 1,3-butylene glycol 7.0 glycerin 1.0 D-panthenol 0.1 Magnesium aluminum silicate 0.3 PEG-40 Stearate 1.2 Stearic Acid 2.0 polysorbate 60 1.5 Lipophilic Glyceryl Stearate 2.0 Sorbitan sesquioleate 1.5 Cetearyl Alcohol 3.0 mineral oil 4.0 squalene 3.8 Red ginseng sugar syrup fermented product of Preparation Example 3 1.5 vegetable oil 1.8 dimethicone 0.4 dipotassium glycyrrhizate a very small amount allantoin a very small amount sodium hyaluronate a very small amount Tocopheryl Acetate Appropriate amount triethanolamine Appropriate amount flavoring agent Appropriate amount Purified water balance

8-4. manufacture of lotion

A lotion containing the fermented red ginseng sugar syrup of Preparation Example 3 as an active ingredient was prepared according to the composition shown in Table 6 below.

Raw material Content (parts by weight) Cetostearyl Alcohol 1.6 stearic acid 1.4 Glycerin lipophilic monostearate 1.8 PEG-100 Stearate 2.6 Sorbital Sesquioleate 0.6 squalene 4.8 macadamia oil 2 jojoba oil 2 Tocopherol Acetate 0.4 Methylpolysiloxane 0.2 Tocopherol Acetate 0.4 1,3-butylene glycol 4 xanthan gum 0.1 glycerin 4 d-pandenol 0.15 Red ginseng sugar syrup fermented product of Preparation Example 3 1.0 allantoin 0.1 Carbomer (2% aq. Sol) 4 triethanolamine 0.15 ethanol 3 Purified water Appropriate amount

Preparation Example 9. Preparation of Beverage

9-1. Manufacture of health drinks

Instantaneous After sterilization, it was prepared by packaging in small packaging containers such as glass bottles and plastic bottles.

9-2. Preparation of vegetable juice

Vegetable juice was prepared by adding 5 g of the fermented red ginseng sugar soak solution of Preparation Example 3 to 1,000 ml of tomato or carrot juice.

9-3. manufacture of fruit juice

1 g of the fermented red ginseng sugar syrup of Preparation Example 3 was added to 1,000 ml of apple or grape juice to prepare fruit juice.

Claims (7)

A composition for improving skin anti-aging comprising a fermented red ginseng sugar syrup obtained by inoculating and fermenting a Saccharomyces strain in red ginseng sugar syrup. The method of claim 1,
The Saccharomyces strain is Saccharomyces cerevisiae Saccharomyces cerevisiae ( Saccharomyces cerevisiae ) The strain composition. The method of claim 1,
The red ginseng sugar syrup is a composition comprising honey and red ginseng-derived ingredients. The method of claim 1,
The red ginseng sugar syrup has a sugar content of 10 brix or less. The method of claim 1,
The red ginseng syrup is a composition in which the content of 11 ginsenosides is 3 to 25 μg/ml. The method of claim 1,
The composition is a cosmetic or health functional food composition composition. A composition for promoting skin regeneration comprising a fermented red ginseng sugar syrup obtained by inoculating and fermenting a Saccharomyces strain in red ginseng sugar syrup.

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