KR20130043438A - Composition for skin wrinkle improvement comprising extracts of junglans mandshurica maxim as an active ingredient - Google Patents
Composition for skin wrinkle improvement comprising extracts of junglans mandshurica maxim as an active ingredient Download PDFInfo
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- KR20130043438A KR20130043438A KR1020110107569A KR20110107569A KR20130043438A KR 20130043438 A KR20130043438 A KR 20130043438A KR 1020110107569 A KR1020110107569 A KR 1020110107569A KR 20110107569 A KR20110107569 A KR 20110107569A KR 20130043438 A KR20130043438 A KR 20130043438A
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- extract
- sputum
- skin
- active ingredient
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/185—Magnoliopsida (dicotyledons)
- A61K36/52—Juglandaceae (Walnut family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q1/00—Make-up preparations; Body powders; Preparations for removing make-up
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2250/00—Food ingredients
- A23V2250/20—Natural extracts
- A23V2250/21—Plant extracts
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Animal Behavior & Ethology (AREA)
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- Veterinary Medicine (AREA)
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- Chemical & Material Sciences (AREA)
- Birds (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Medicinal Chemistry (AREA)
- Pharmacology & Pharmacy (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
Description
본 발명은 가래나무(Junglans mandshurica MAXIM) 추출물을 유효성분으로 함유하는 피부 주름 개선용 화장료 조성물, 피부 노화방지용 화장료 조성물, 피부보호용 화장료 조성물 및 색조 화장료 조성물에 관한 것이다.
The present invention is sputum ( Junglans The present invention relates to a cosmetic composition for improving skin wrinkles, a skin anti-aging cosmetic composition, a skin protective cosmetic composition, and a color cosmetic composition containing an extract of mandshurica MAXIM) as an active ingredient.
사람의 피부는 크게 표피층, 진피층, 피하지방층으로 구성되어 있으며, 피부의 가장 기본적인 역할은 우리 몸으로부터 수분이 증발되는 것을 막아주며 외부로부터 유해성분이 침입하는 것을 막아주는 역할을 한다. 이러한 피부는 항상 외부 자극으로부터 노출되어 있기 때문에 다양한 방어 능력을 가지고 있다. 또한, 피부가 건강하고 깨끗함은 곧 사람의 아름다움과 연관되어 있다고 할 수 있다. 최근 들어, 여성뿐 아니라 남성들도 피부 노화 등 여러 가지 피부 트러블들에 대한 관심이 많아지면서 이와 관련된 제품들이 많이 개발되고 있다.The skin of the human skin consists largely of the epidermis, the dermis, and the subcutaneous fat layer. The basic role of the skin is to prevent water from evaporating from our body and to prevent harmful substances from invading from the outside. These skin are always exposed to external stimuli, so they have various defenses. Also, the health and cleanness of skin is related to human beauty. Recently, not only women but also men are interested in various skin problems such as skin aging has been developed a lot of related products.
사람은 나이가 들면서 피부노화가 일어나게 되는데 그 대표적인 증상이 주름(wrinkle)이다. 주름은 나이를 나타내는 하나의 현상으로서, 주름이 생기는 대표적인 원인은 피부의 진피에서 매트릭스를 형성하는 콜라겐의 분해에 기인한다. 피부의 콜라겐은 노화가 진행됨에 따라 생성이 저하되기도 하나, 자외선 등의 외부 자극에 의해 콜라겐 분해 효소(Matrix Metallo Proteinase, MMP-1)의 활성도가 높아지면서 콜라겐이 쉽게 분해되어 주름의 생산이 증가하는 임상 보고들도 많다. 따라서, 최근에는 MMP-1의 생성의 저해 및 활성을 억제하여 근본적으로 생성되는 콜라겐의 분해를 억제하는 제품들도 많이 개발되고 있다.
As a person ages, skin aging occurs. Wrinkle is a representative symptom. Wrinkles are a phenomenon of age, and a representative cause of wrinkles is due to the breakdown of collagen, which forms a matrix in the dermis of the skin. Collagen in the skin decreases as aging progresses.However, the activity of collagen decomposing enzyme (Matrix Metallo Proteinase, MMP-1) is increased by external stimulation such as ultraviolet rays. There are many clinical reports. Therefore, in recent years, many products have been developed that inhibit the production and inhibition of collagen produced by inhibiting the production and inhibition of the production of MMP-1.
MMP는 다형핵성호중구(Polymorphonuclear neutrophil), 대식세포(Macrophage), 섬유아세포(Fibroblast) 등과 같은 세포로부터 분비되는 칼슘 및 아연 의존성 엔도펩티다제(Endopeptidase)로 중성 pH에서 작용하며, 기질로서 여러 가지 세포의 기질을 이용한다. 주로 피부에서는 MMP-1, MMP-2, MMP-9 이외에 여러 가지 효소들이 작용을 하는데 콜라겐 분해와 관련되어 가장 근본적으로 작용하는 효소는 MMP-1이다. 일반적으로 콜라겐 합성을 촉진하는 종래의 물질로는 레티노이드(RE36068), TGF-β(transforming growth factor), 베툴린산(JP8-208424) 등이 있으며, MMP-1의 생성을 억제하는 물질로는 TGF-β(transforming growth factor)가 대표적으로 알려져 있으며, 그 외에도 다양한 천연물들이 MMP-1 생합성을 억제하는 것으로 알려져 있다. 따라서, 주름 생성을 억제하는 방법은 콜라겐 합성을 촉진하거나, 또는 콜라겐 분해효소의 생성 및 활성을 억제하는 방법을 사용할 수 있으며, 보다 효과적이고 용이하며 안전한 주름개선을 위한 화장료의 개발이 절실이 요구되고 있다.
MMP is a calcium and zinc-dependent endopeptidase secreted from cells such as polymorphonuclear neutrophil, macrophage, fibroblast, etc., and it acts at neutral pH. Use the substrate of the cell. In the skin, MMP-1, MMP-2, and MMP-9, in addition to a variety of enzymes work, the most fundamental enzyme associated with collagen degradation is MMP-1. In general, conventional substances that promote collagen synthesis include retinoids (RE36068), transforming growth factor (TGF-β), betulinic acid (JP8-208424), and the like. TGF- inhibits the production of MMP-1. β (transforming growth factor) is representatively known, and various other natural products are known to inhibit MMP-1 biosynthesis. Therefore, the method of inhibiting wrinkle formation may be a method of promoting collagen synthesis or inhibiting the production and activity of collagen degrading enzymes, and the development of cosmetics for more effective, easy and safe wrinkle improvement is urgently needed. have.
가래나무(Junglans mandshurica MAXIM)는 가래나무목 가래나무과의 낙엽 활엽 교목으로, 한국(중부 이북),·중국 북동부, 시베리아(아무르·우수리) 등지에 많이 서식하는 나무로서 추자목(楸子木)이라고도 하고 열매를 추자(楸子)라 한다. 산기슭의 양지쪽에서 자라고 높이가 20m 정도이며 나무껍질은 암회색이며 세로로 터진다. 잎은 홀수 깃꼴겹 잎이다. 작은 잎은 7～17개이며, 긴 타원형 또는 달걀 모양 타원형으로 길이 7～28 cm, 나비 10 cm 정도이다. 잔 톱니가 있고 앞면은 잔털이 있으나 점차 없어지고, 뒷면은 털이 있거나 없는 것도 있으며 잎맥 위에 선모(腺毛)가 있다. 꽃은 단성화로서 4월에 피는데, 수꽃이삭은 길이 10～20 cm이고, 수술은 12～14개이며 암꽃이삭에 4～10개의 꽃이 핀다. 열매는 핵과로서 달걀 모양 원형이고, 길이가 4～8 cm이며 9월에 익는다. 외과피에는 선모가 빽빽히 나고, 내과피는 흑갈색인데 매우 굳으며 양끝이 뾰족하다. 나무의 변재는 회백색, 심재는 회갈색으로 질이 치밀하고 질기며 뒤틀리지 않아 가구재·기계재·총대·조각재로 쓰인다.Azusa (Junglans mandshurica MAXIM) is a deciduous broad-leaved arboreous tree of the sputum barkaceae family, which inhabits many places in Korea (central north), northeastern China, and Siberia (Amur and Usuri).楸 子) is called. It grows on both sides of the foothills and is about 20m high. The bark is dark gray and it grows vertically. The leaves are odd pinnate leaves. Small leaves 7-17, long oval or egg-shaped oval, 7-28 cm long, 10 cm butterfly. There are fine teeth and the front side has fine hairs, but gradually disappears, the back side has hairs or no hairs, and there is a hair line on the leaf veins. Flowers are single flowers and bloom in April. Male flower spikes are 10-20 cm long, 12-14 flower stamens, and 4-10 flowers bloom in female spikes. Fruits are nucleus, oval round, 4 ~ 8 cm long, ripen in September. Surgical epidermis densely packed, inner epidermis dark brown, very hard and pointed at both ends. Sapwood of wood is grayish white and heartwood is grayish brown. It is used for furniture, machinery, forearm and sculpture as it is dense, tough and not warped.
한방에서는 봄에서 가을 사이에 수피를 채취하여 말린 것을 추피(楸皮)라 하며 수렴과 해열, 눈을 맑게 하는 등의 효능이 있어 장염·이질(적리)·설사·맥립종, 눈이 충혈하고 붓는 통증 등에 처방한다. 열매는 날 것으로 그냥 먹거나 요리하여 먹고, 기름을 짜서 먹기도 한다. 어린 잎은 삶아서 먹을 수 있다. 수피는 섬유로도 사용한다. 핵과가 긴 타원형이고 양끝이 좁으며 능각(稜角)이 다소 뚜렷하지 않은 것을 긴가래나무(J.m.for.stenocarpa)라고 하고, 핵과에 능선(稜線)이 없고 하나의 꽃이삭에 암꽃이 12～20개씩 달리는 것을 왕가래나무 (J.m.var. sieboldiana)라고 한다. 가래 열매는 호두와 마찬가지로 폐를 튼튼하게 하고 기침을 멎게 하며 기억력을 좋게 하고 머리를 맑게 하는 등의 약효가 있으나 민간 의학에서는 가래 열매 보다는 가래나무 껍질을 추목피(楸木皮)라 하여 약으로 더 많이 사용되며, 가래나무 껍질은 항암 작용이 뛰어난 것으로 알려져 있다. 한편, 대한민국 공개특허 제10-2011-0093754 (공개일 2011년 08월 18일)에는 가래나무 열매의 과즙을 이용하여 식물 유래 천연 염색제의 유효성분으로 이용될 수 있음을 개시하고 있으나, 아직까지 가래나무 추출물의 항주름 효과에 대해서는 밝혀진바 없다.
In oriental medicine, bark is taken from spring to autumn and dried. It is called bleeding, and it is effective in converging, fevering, and clearing eyes. Enteritis, dysentery, diarrhea, gynomas, and redness and swelling pain. Prescribe on back. Fruits are raw and can be eaten or cooked or squeezed. Young leaves can be boiled and eaten. Bark is also used as a fiber. Nucleus is long oval, narrow at both ends, and the ridge is not clear. It is called long phlegm (Jmfor.stenocarpa). This is called a sputum (Jmvar. Sieboldiana). As with walnuts, sputum berries have the effect of strengthening the lungs, coughing, improving memory, and clearing the head, like walnuts. However, in folk medicine, sputum bark is called Chukpipi (楸 木皮) more than medicine. Sputum bark is known to have excellent anticancer activity. On the other hand, the Republic of Korea Patent Publication No. 10-2011-0093754 (published August 18, 2011) discloses that it can be used as an active ingredient of a plant-derived natural dye using the juice of the sputum tree fruit, but still sputum The anti-wrinkle effect of tree extracts is not known.
이에 본 발명자들은 피부 주름 개선을 위한 천연 식물 추출물을 개발하고자 연구한 결과, 가래나무 잎 추출물이 세포 보호효과, ROS(Reactive oxygen species) 생성 억제효과, 콜라겐 분해 효소인 MMP-1(Matrix metalloproteinases-1)의 활성 억제효과 및 콜라겐 생합성을 증가시키는 COL1A1(pro-collagen type 1)의 발현 증가 효과를 가지므로, 이를 피부 주름 개선, 피부 노화방지, 피부 보호 및 색조 화장료 조성물의 유효성분으로 유용하게 사용할 수 있음을 확인하고 본 발명을 완성하였다.
Therefore, the present inventors have studied to develop a natural plant extract for improving skin wrinkles, as a result, the extract of sputum tree leaf cell protection effect, inhibitory effect of ROS (Reactive oxygen species) production, collagen degrading enzyme MMP-1 (Matrix metalloproteinases-1 ) Has the effect of increasing the expression of COL1A1 (pro-collagen type 1), which inhibits the activity and increases collagen biosynthesis, and thus can be usefully used as an active ingredient in skin wrinkle improvement, skin anti-aging, skin protection, and color cosmetic composition. It was confirmed that the present invention was completed.
본 발명은 목적은, 가래나무(Junglans mandshurica MAXIM) 추출물을 유효성분으로 함유하는 피부 주름 개선용 화장료 조성물, 피부주름 개선용 약학적 조성물, 피부 주름 개선용 건강식품용 조성물 및 피부 주름개선용 피부 외용제를 제공하는 것이다.The object of the present invention, the phlegm ( Junglans It is to provide a cosmetic composition for improving skin wrinkles, a pharmaceutical composition for improving skin wrinkles, a composition for health foods for improving skin wrinkles and a skin external preparation for improving skin wrinkles containing mandshurica MAXIM) extract as an active ingredient.
본 발명의 또 다른 목적은 가래나무 추출물을 유효성분으로 함유하는 피부 노화방지용 화장료 조성물, 피부 노화방지용 약학적 조성물, 피부 노화방지용 건강식품용 조성물 및 피부 노화방지용 피부 외용제를 제공하는 것이다.Still another object of the present invention is to provide a skin anti-aging cosmetic composition, a skin anti-aging pharmaceutical composition, a skin anti-aging health food composition, and a skin anti-aging skin composition containing a sputum extract as an active ingredient.
본 발명의 또 다른 목적은 가래나무 추출물을 유효성분으로 함유하는 피부 보호용 화장료 조성물, 피부 보호용 약학적 조성물, 피부 보호용 건강식품용 조성물 및 피부 보호용 피부 외용제를 제공하는 것이다.Still another object of the present invention is to provide a skin protective cosmetic composition, a skin protective pharmaceutical composition, a skin protective health food composition, and a skin external preparation containing the sputum extract as an active ingredient.
본 발명의 또 다른 목적은 가래나무 추출물을 함유하는 색조 화장료 조성물을 제공하는 것이다.
Still another object of the present invention is to provide a color cosmetic composition containing the extract of phlegm.
상기 목적을 달성하기 위하여, 본 발명은 가래나무(Junglans mandshurica MAXIM) 추출물을 유효성분으로 함유하는 피부 주름 개선용 화장료 조성물을 제공한다.In order to achieve the above object, the present invention is a sputum ( Junglans) It provides a cosmetic composition for improving skin wrinkles containing mandshurica MAXIM) extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 주름 개선용 약학적 조성물을 제공한다.In addition, the present invention provides a pharmaceutical composition for improving skin wrinkles containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 주름 개선용 건강식품용 조성물을 제공한다.In another aspect, the present invention provides a composition for improving skin wrinkles containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 주름 개선용 피부 외용제를 제공한다.In addition, the present invention provides a skin external preparation for improving skin wrinkles containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 노화방지용 화장료 조성물을 제공한다.The present invention also provides a cosmetic composition for preventing skin aging containing the extract of phlegm as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 노화방지용 약학적 조성물을 제공한다.The present invention also provides a pharmaceutical composition for preventing skin aging containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 노화방지용 건강식품용 조성물을 제공한다.In addition, the present invention provides a composition for preventing skin aging health food containing the extract of phlegm as an active ingredient.
또한, 또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 노화방지용 피부 외용제를 제공한다.In addition, the present invention provides a skin external preparation for preventing skin aging containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 보호용 화장료 조성물을 제공한다.The present invention also provides a cosmetic composition for skin protection containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 보호용 약학적 조성물을 제공한다.The present invention also provides a pharmaceutical composition for protecting the skin containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 보호용 건강식품용 조성물을 제공한다.In addition, the present invention provides a composition for health food for skin protection containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 보호용 피부 외용제를 제공한다.In addition, the present invention provides a skin external preparation for skin protection containing a sputum extract as an active ingredient.
아울러, 본 발명은 가래나무 추출물을 함유하는 색조 화장료 조성물을 제공한다.
In addition, the present invention provides a color cosmetic composition containing a sputum extract.
본 발명의 가래나무 추출물은 세포 보호효과, ROS(Reactive oxygen species) 생성 억제효과, 콜라겐 분해 효소인 MMP-1(Matrix metalloproteinases-1)의 활성 억제 효과 및 피부세포에서 콜라겐 생합성을 증가시켜 피부의 주된 구성성분이 콜라겐을 증가시키는 COL1A1(pro-collagen type 1)의 발현 증가 효과를 가지므로 상기 가래나무 잎 추출물을 피부 주름 개선용, 피부 노화방지용, 피부 보호용 및 색조 화장료 조성물에 유용하게 사용될 수 있음을 확인하였다.
The extract of the sputum tree of the present invention is to protect the cells, inhibit the production of reactive oxygen species (ROS), inhibit the activity of collagen degrading enzyme MMP-1 (Matrix metalloproteinases-1) and increase collagen biosynthesis in skin cells Since the component has an effect of increasing the expression of COL1A1 (pro-collagen type 1) which increases collagen, the sputum leaf extract may be useful for improving skin wrinkles, anti-aging, skin protection, and color cosmetic composition. Confirmed.
도 1은 HS68 세포주에서 가래나무 잎 추출물의 세포 보호효과를 확인한 도이다;
JWLE: 가래나무 잎 추출물 처리군;
NAC: 양성대조군인 N-아세틸시스테인(N-Acetyl-L-Cysteine) 처리군; 및
GREEN tea: 양성대조군인 녹차 처리군.
도 2는 HS68 세포주에서 H2O2 자극에 대한 가래나무 추출물의 세포 보호효과를 나타낸 도이다;
JWLE: 가래나무 잎 추출물 처리군;
NAC: N-아세틸시스테인(N-Acetyl-L-Cysteine) 처리군; 및
GREEN tea: 녹차 처리군.
도 3A는 HS68 세포주에서 H2O2 자극에 의해 과생성되는 ROS(Reactive Oxygen Species)에 대한 가래나무 잎 추출물의 효과를 나타낸 도이다;
JWLE: 가래나무 잎 추출물 처리군; 및
GREEN tea: 녹차 처리군.
도 3B는 현광 현미경으로 확인한 세포 사진을 나타낸 도이다;
Control: 무처리군;
H2O2: H2O2 처리군;
GREEN tea + H2O2: 녹차 및 H2O2 동시처리군;
JWLE 50 + H2O2: 본 발명의 가래나무 잎 추출물 50 ug/ml 및 H2O2 동시처리군;
JWLE 100 + H2O2: 본 발명의 가래나무 잎 추출물 100 ug/ml 및 H2O2 동시처리군; 및
JWLE 200 + H2O2: 본 발명의 가래나무 잎 추출물 200 ug/ml 및 H2O2 동시처리군.
도 4는 HS68 세포주에서 H2O2 자극에 의해 증가한 MMP-1(Matrix metalloproteinases-1)에 대한 가래나무 잎 추출물의 효과를 나타낸 도이다;
JWLE: 가래나무 잎 추출물 처리군;
NAC: N-아세틸시스테인(N-Acetyl-L-Cysteine) 처리군; 및
GREEN tea: 녹차 처리군.
도 5는 HS68 세포주에서 H2O2 자극에 의해 감소한 COL1A1(pro-collagen type 1)에 대한 가래나무 잎 추출물의 효과를 나타낸 도이다;
JWLE: 가래나무 잎 추출물 처리군; 및
GREEN tea: 녹차 처리군.1 is a diagram confirming the cell protective effect of the extract of Sapberry leaves in HS68 cell line;
JWLE: sputum leaf extract treatment group;
NAC: N-Acetyl-L-Cysteine treated group, a positive control group; And
GREEN tea: Green tea treated group with positive control.
Figure 2 is a diagram showing the cytoprotective effect of the bark extract against H 2 O 2 stimulation in HS68 cell line;
JWLE: sputum leaf extract treatment group;
NAC: N-Acetyl-L-Cysteine treatment group; And
GREEN tea: Green tea treated group.
3A is a diagram showing the effect of sputum leaf extract on ROS (Reactive Oxygen Species) overproduced by H 2 O 2 stimulation in HS68 cell line;
JWLE: sputum leaf extract treatment group; And
GREEN tea: Green tea treated group.
3B is a diagram showing a cell photograph confirmed by a fluorescence microscope;
Control: no treatment group;
H 2 O 2 : H 2 O 2 treated group;
GREEN tea + H 2 O 2 : green tea and H 2 O 2 simultaneous treatment group;
4 is a diagram showing the effect of sputum leaf extract on MMP-1 (Matrix metalloproteinases-1) increased by H 2 O 2 stimulation in HS68 cell line;
JWLE: sputum leaf extract treatment group;
NAC: N-Acetyl-L-Cysteine treatment group; And
GREEN tea: Green tea treated group.
5 is a diagram showing the effect of sputum leaf extract on COL1A1 (pro-collagen type 1) reduced by H 2 O 2 stimulation in HS68 cell line;
JWLE: sputum leaf extract treatment group; And
GREEN tea: Green tea treated group.
이하, 본 발명을 상세히 설명한다.
Hereinafter, the present invention will be described in detail.
본 발명은 가래나무(Junglans mandshurica MAXIM) 추출물을 유효성분으로 함유하는 피부 주름 개선용 화장료 조성물을 제공한다. The present invention is sputum ( Junglans It provides a cosmetic composition for improving skin wrinkles containing mandshurica MAXIM) extract as an active ingredient.
상기 가래나무 추출물은 하기의 단계들을 포함하는 제조방법에 의해 제조되는 것이 바람직하나 이에 한정하지 않는다: The phlegm extract is preferably prepared by a manufacturing method comprising the following steps, but not always limited thereto:
1) 가래나무에 추출용매를 가하여 추출하는 단계;1) extracting by adding an extraction solvent to the sputum tree;
2) 단계 1)의 추출물을 식힌 후 여과하는 단계; 2) cooling the extract of step 1) and filtering;
3) 단계 2)의 여과한 추출물을 감압농축하는 단계; 및3) concentrating the filtered extract of step 2) under reduced pressure; And
4) 단계 3)의 농축된 추출물을 건조 분말화하는 단계.4) dry-pulverizing the concentrated extract of step 3).
상기 방법에 있어서, 단계 1)의 가래나무는 재배한 것 또는 시판되는 것 등 제한 없이 사용할 수 있다. In the above method, the phlegm of step 1) can be used without limitation, such as cultivated or commercially available.
상기 가래나무는 가래나무 가지, 줄기, 뿌리 또는 잎을 이용하는 것이 바람직하고, 가래나무 잎을 이용하는 것이 가장 바람직하나 이에 한정하지 않는다.The sputum tree is preferably using a sputum branch, stem, root or leaf, it is most preferred to use a sputum leaf, but is not limited thereto.
상기 추출용매는 물, 알코올 또는 이들의 혼합물을 사용하는 것이 바람직하나, 이에 한정하지 않는다. 상기 알코올로는 C1 내지 C4의 저급 알코올을 이용하는 것이 바람직하며, 저급 알코올로는 에탄올 또는 메탄올을 이용하는 것이 바람직하나, 이에 한정하지 않는다. 추출방법으로는 진탕 추출 또는 환류 추출을 이용하는 것이 바람직하나 이에 한정하지 않는다. 상기 추출 용매를 가래나무 분량에 2 내지 10배 첨가하여 추출하는 것이 바람직하나 이에 한정하지 않는다. 추출온도는 상온 내지 100℃인 것이 바람직하나 이에 한정하지 않는다. 또한, 추출시간은 2 내지 5일인 것이 바람직하나 이에 한정하지 않는다. 아울러, 추출 회수는 1 내지 3회인 것이 바람직하나 이에 한정하지 않는다. The extraction solvent is preferably, but not limited to, water, alcohol or a mixture thereof. As the alcohol, it is preferable to use C 1 to C 4 lower alcohols, and as the lower alcohol, ethanol or methanol is preferably used, but is not limited thereto. As the extraction method, it is preferable to use shaking extraction or reflux extraction, but not always limited thereto. The extraction solvent is preferably extracted by adding 2 to 10 times the amount of sputum tree, but not always limited thereto. The extraction temperature is preferably from room temperature to 100 ° C, but is not limited thereto. The extraction time is preferably 2 to 5 days, but is not limited thereto. The number of times of extraction is preferably 1 to 3 times, but is not limited thereto.
상기 방법에 있어서, 단계 3)의 감압농축은 진공감압농축기 또는 진공회전증발기를 이용하는 것이 바람직하나 이에 한정하지 않는다. 또한, 단계 4)의 건조는 감압건조, 진공건조, 비등건조, 분무건조 또는 동결건조하는 것이 바람직하나 이에 한정하지 않는다.
In the above method, the decompression concentration in step 3) preferably uses a vacuum decompression concentrator or a vacuum rotary evaporator, but is not limited thereto. In addition, the drying of step 4) is preferably reduced pressure drying, vacuum drying, boiling drying, spray drying or freeze drying, but not always limited thereto.
본 발명의 구체적인 실시예에서, 가래나무 잎에 70% 에탄올을 시료의 10배 양으로 상온에서 침출시켜 감압 여과하여, 가래나무 추출물을 얻었다. In a specific embodiment of the present invention, 70% ethanol was leached to 10 times the amount of the sample on a sputum tree leaf at room temperature and filtered under reduced pressure to obtain a sputum tree extract.
또한, 본 발명의 또 다른 구체적인 실시예에서, HS68 세포에서 상기 제조된 가래나무 추출물의 세포 보호효과를 확인한 결과, 본 발명의 가래나무 추출물을 전처리는 세포 생존율에 영향을 미치지 않는 것을 확인하였다(도 1 참조).In addition, in another specific embodiment of the present invention, as a result of confirming the cell protective effect of the prepared sputum bark extract in HS68 cells, it was confirmed that pretreatment of the sputum bark extract of the present invention does not affect the cell viability (Fig. 1).
또한, 본 발명의 또 다른 구체적인 실시예에서, HS68 세포에서 상기 제조된 가래나무 추출물의 H2O2 자극에 대한 세포 보호 효과를 확인한 결과, 본 발명의 가래나무 추출물의 농도의존적으로 유의적인 세포 보호효과를 나타내는 것을 확인하였다(도 2 참조). In addition, in another specific embodiment of the present invention, as a result of confirming the cytoprotective effect on the H 2 O 2 stimulation of the prepared sputum bark extract in HS68 cells, the concentration-dependent cell protection of the sputum bark extract of the present invention It confirmed that the effect was shown (refer FIG. 2).
또한, 본 발명의 또 다른 구체적인 실시예에서, HS68 세포에서 상기 제조된 가래나무 추출물의 H2O2에 의해 증가한 ROS(Reactive Oxygen Species) 과생성에 대한 효과를 확인한 결과, 본 발명의 가래나무 추출물은 H2O2에 의해 증가한 ROS의 과생성을 농도의존적으로 억제하는 것을 확인하였다(도 3 참조).In addition, in another specific embodiment of the present invention, as a result of confirming the effect on ROS (Reactive Oxygen Species) overproduction increased by H 2 O 2 of the prepared sputum bark extract in HS68 cells, the sputum bark extract of the present invention It was confirmed that the concentration-dependently inhibit the overproduction of ROS increased by H 2 O 2 (see FIG. 3).
또한, 본 발명의 또 다른 구체적인 실시예에서, HS68 세포에서 상기 제조된 가래나무 추출물의 H2O2에 의해 증가하는 MMP-1(Matrix Metallo Proteinase)의 억제 효과를 확인한 결과, 본 발명의 가래나무 추출물 전처리는 증가한 MMP-1 농도의존적으로 억제하는 것을 확인하였다. (도 4 참조). In addition, in another specific embodiment of the present invention, as a result of confirming the inhibitory effect of MMP-1 (Matrix Metallo Proteinase) increased by H 2 O 2 of the prepared sputum tree extract in HS68 cells, the sputum tree of the present invention Extract pretreatment was confirmed to inhibit dependently on increased MMP-1 concentration. (See Figure 4).
또한, 본 발명의 또 다른 구체적인 실시예에서, HS68 세포에서 상기 제조된 가래나무 추출물의 H2O2에 의해 감소한 COL1A1(Pro-collagen type 1)의 증가효과를 확인한 결과, 본 발명의 가래나무 추출물은 감소한 COL1A1의 유의적인 증가효과를 나타내는 것을 확인하였다(도 5 참조). In addition, in another specific embodiment of the present invention, as a result of confirming the effect of increasing the COL1A1 (Pro-collagen type 1) reduced by H 2 O 2 of the prepared sputum bark extract in HS68 cells, the sputum bark extract of the present invention Was confirmed to show a significant increase effect of reduced COL1A1 (see Figure 5).
따라서, 가래나무 추출물은 우수한 세포보호 효과, ROS 억제 효과, MMP-1의 억제 효과 및 COL1A1 보호 효과를 가지고 있어, 주름 억제 효과가 우수하므로, 피부 주름 개선에 효과적인 화장료 조성물로서 유용하게 사용될 수 있다.
Therefore, the extract of phlegm has excellent cytoprotective effect, ROS inhibitory effect, MMP-1 inhibitory effect and COL1A1 protective effect, and excellent anti-wrinkle effect, it can be usefully used as a cosmetic composition effective to improve skin wrinkles.
상기 화장료 조성물은, 예를 들면 용액, 겔, 고체 또는 반죽 무수 생성물, 수상에 유상을 분산시켜 얻은 에멀젼, 현탁액, 마이크로에멀젼, 마이크로캡슐, 미세과립구 또는 이온형(리포좀), 비이온형의 소낭 분산제의 형태, 크림, 스킨, 로션, 파우더, 연고, 스프레이 또는 콘실 스틱의 형태로 제공될 수 있다. 또한, 포말(foam)의 형태 또는 압축된 추진제를 더 함유한 에어로졸 조성물의 형태로도 제조될 수 있다.The cosmetic composition may be in the form of a solution, a gel, a solid or a paste anhydrous product, an emulsion obtained by dispersing an oil phase in water, a suspension, a microemulsion, a microcapsule, a microgranule or an ionic (liposome) A cream, a skin, a lotion, a powder, an ointment, a spray, or a cone stick. It may also be prepared in the form of a foam or in the form of an aerosol composition further containing a compressed propellant.
또한, 상기 화장료 조성물은 본 발명의 가래나무 추출물에 추가로 지방 물질, 유기 용매, 용해제, 농축제 및 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온봉쇄제 및 킬레이트화제, 보존제, 비타민, 차단제, 습윤화제, 필수 오일, 염료, 안료, 친수성 또는 친유성 활성제, 지질 소낭 또는 화장품에 통상적으로 사용되는 임의의 다른 성분과 같은 화장품 분야에서 통상적으로 사용되는 보조제를 함유할 수 있다.In addition, the cosmetic composition is in addition to the phlegm extract of the present invention, fatty substances, organic solvents, solubilizers, thickening and gelling agents, softeners, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, surfactants Commonly used in water, ionic or nonionic emulsifiers, fillers, metal-ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or cosmetics It may contain adjuvants conventionally used in the cosmetic field such as any other ingredients used.
본 발명의 가래나무 추출물을 함유하는 화장료 조성물에 있어서, 통상적으로 함유되는 화장료 조성물에 본 발명의 추출물이 1 내지 15 중량%, 바람직하게는 2 내지 10 중량%의 양으로 첨가될 수 있다.
In the cosmetic composition containing the sputum extract of the present invention, the extract of the present invention may be added to the cosmetic composition usually contained in an amount of 1 to 15% by weight, preferably 2 to 10% by weight.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 주름 개선용 약학적 조성물을 제공한다.In addition, the present invention provides a pharmaceutical composition for improving skin wrinkles containing a sputum extract as an active ingredient.
상기 가래나무는 가래나무 가지, 줄기, 뿌리 또는 잎을 이용하는 것이 바람직하고, 가래나무 잎을 이용하는 것이 가장 바람직하나 이에 한정하지 않는다.The sputum tree is preferably using a sputum branch, stem, root or leaf, it is most preferred to use a sputum leaf, but is not limited thereto.
본 발명의 가래나무 추출물은 우수한 세포보호 효과, ROS 억제 효과, MMP-1의 억제 효과 및 COL1A1 보호 효과를 가지고 있어, 주름 억제 효과가 우수하므로, 피부 주름 개선용 약학적 조성물로서 유용하게 사용될 수 있다.Sputum bark extract of the present invention has an excellent cytoprotective effect, ROS inhibitory effect, MMP-1 inhibitory effect and COL1A1 protective effect, excellent anti-wrinkle effect, can be usefully used as a pharmaceutical composition for improving skin wrinkles .
본 발명의 약학적 조성물은 통상적으로 사용되는 부형제, 붕해제, 감미제, 활택제, 향미제 등을 추가로 포함할 수 있으며, 통상적인 방법에 의해 정제, 캅셀제, 산제, 과립제, 현탁제, 유제, 시럽제, 기타 액제로 제형화될 수 있다.The pharmaceutical composition of the present invention may further contain commonly used excipients, disintegrants, sweeteners, lubricants, flavors and the like, and may be formulated into tablets, capsules, powders, granules, suspensions, Syrups, and other liquid preparations.
구체적으로 본 발명의 약학적 조성물은 경구 투여용 제형, 예를 들면 정체, 트로치제(troches), 로젠지(lozenge), 수용성 또는 우성현탁액, 조제분말 또는 과립, 에멀젼, 하드 또는 소프트 캡슐, 시럽 또는 엘릭시르제(elixirs)로 제제화된다. 정제 및 캡슐 등의 제형으로 제제하기 위해 락토오스, 사카로오스, 솔비톨, 만니톨, 전분, 아밀로펙틴, 셀롤로오스 또는 젤라틴과 같은 결합제, 디칼슘 포스페이트와 같은 부형제, 옥수수 전분 또는 고구마 전분과 같은 붕해제, 스테아르산 마그네슘, 스테아르산 칼슘, 스테아릴푸마르산 나트륨 또는 폴리에틸렌글리콜 왁스와 같은 윤활유가 함유된다. 캡슐제형의 경우는 상기에서 언급한 물질 이외에도 지방유와 같은 액체 담체를 함유한다.Specifically, the pharmaceutical compositions of the present invention can be formulated for oral administration, for example, as tablets, troches, lozenges, aqueous or aqueous suspensions, prepared powders or granules, emulsions, hard or soft capsules, It is formulated into elixirs. Binders such as lactose, saccharose, sorbitol, mannitol, starch, amylopectin, celluloses or gelatin, excipients such as dicalcium phosphate, disintegrants such as corn starch or sweet potato starch, and disintegrants such as stearic acid Magnesium stearate, calcium stearate, sodium stearyl fumarate, or polyethylene glycol wax. In the case of a capsule formulation, in addition to the above-mentioned substances, a liquid carrier such as fatty oil is contained.
또한, 본 발명의 약학적 조성물은 경구 또는 비경구 투여할 수 있으며, 비경구 투여시 피하주사, 정맥주사, 근육내 주사 또는 흉부내 주사 주입방식을 선택하는 것이 바람직하다. 비경구 투여용 제형으로 제제화하기 위해서는 본 발명의 가래나무 추출물을 안정제 또는 완충제와 함께 물에서 혼합하여 현탁액으로 제조하고 이를 앰플 또는 바이알의 단위 투여형으로 제제한다.In addition, the pharmaceutical composition of the present invention can be administered orally or parenterally, and it is preferable to select subcutaneous injection, intravenous injection, intramuscular injection, or intra-thoracic injection injection method for parenteral administration. To formulate into a parenteral formulation, the extract of the phlegm of the present invention is prepared in suspension by mixing in water with a stabilizer or buffer and formulated in a unit dosage form of ampoules or vials.
본 발명에 따른 유효성분의 투여량은 체내에서 활성성분의 흡수도, 불활성화율 및 배설속도, 환자의 연령, 성별 및 상태, 치료할 질병의 중증 정도에 따라 적절히 선택되나, 경구 투여제의 경우 일반적으로 성인에게 1일에 체중 1 ㎏당 본 발명의 추출물을 0.0001 ~ 500 ㎎의 양으로 1회 내지 수회 나누어 투여할 수 있으며, 0.001 ~ 100 ㎎의 양으로 투여하는 것이 바람직하다.
The dosage of the active ingredient according to the present invention is appropriately selected according to the absorption rate, inactivation rate and excretion rate of the active ingredient in the body, the age, sex and condition of the patient, the severity of the disease to be treated, but in the case of oral administration in general Adults can be administered once to several times in an amount of 0.0001 to 500 mg of the extract of the present invention per kg of body weight per day, it is preferable to administer in an amount of 0.001 to 100 mg.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 주름개선용 건강식품용 조성물을 제공한다.In another aspect, the present invention provides a composition for improving skin wrinkles, containing a sputum extract as an active ingredient.
상기 가래나무는 가래나무 가지, 줄기, 뿌리 또는 잎을 이용하는 것이 바람직하고, 가래나무 잎을 이용하는 것이 가장 바람직하나 이에 한정하지 않는다.The sputum tree is preferably using a sputum branch, stem, root or leaf, it is most preferred to use a sputum leaf, but is not limited thereto.
본 발명의 가래나무 추출물은 우수한 세포보호 효과, ROS 억제 효과, MMP-1의 억제 효과 및 COL1A1 보호 효과를 가지고 있어, 피부 주름개선용 건강식품으로서 유용하게 사용될 수 있다.Sputum bark extract of the present invention has an excellent cytoprotective effect, ROS inhibitory effect, MMP-1 inhibitory effect and COL1A1 protective effect, it can be usefully used as a health food for skin wrinkle improvement.
본 발명의 건강식품은 가래나무 추출물을 그대로 첨가하거나 다른 식품 또는 식품 성분과 함께 사용될 수 있고, 통상적인 방법에 따라 적절하게 사용될 수 있다.The health food of the present invention may be added as it is, extract of phlegm or used with other food or food ingredients, and may be appropriately used according to a conventional method.
상기 식품의 종류에는 특별한 제한은 없다. 상기 가래나무 추출물을 첨가할 수 있는 식품의 예로는 육류, 소시지, 빵, 초콜릿, 캔디류, 스넥류, 과자류, 피자, 라면, 기타 면류, 껌류, 아이스크림류를 포함한 낙농제품, 각종 스프, 음료수, 차, 드링크제, 알코올음료 및 비타민 복합제 등이 있으며, 통상적인 의미에서의 건강식품을 모두 포함한다.There is no particular limitation on the kind of the food. Examples of foods to which the extract of the sputum tree can be added include meat, sausage, bread, chocolate, candy, snacks, confectionery, pizza, ramen, other noodles, gums, dairy products including ice cream, various soups, drinks, tea, Drinks, alcoholic beverages and vitamin complexes and the like, and includes all of the health food in the usual sense.
본 발명의 건강 음료 조성물은 통상의 음료와 같이 여러 가지 향미제 또는 천연 탄수화물 등을 추가 성분으로서 함유할 수 있다. 상술한 천연 탄수화물은 포도당, 과당과 같은 모노사카라이드, 말토스, 슈크로스와 같은 디사카라이드, 및 덱스트린, 사이클로덱스트린과 같은 폴리사카라이드, 자일리톨, 소르비톨, 에리트리톨 등의 당알콜이다. 감미제로서는 타우마틴, 스테비아 추출물과 같은 천연 감미제나, 사카린, 아스파르탐과 같은 합성 감미제 등을 사용할 수 있다. 상기 천연 탄수화물의 비율은 본 발명의 조성물 100 ㎖당 일반적으로 약 0.01 ~ 0.04 g, 바람직하게는 약 0.02 ~ 0.03 g 이다.The health beverage composition of the present invention may contain various flavors or natural carbohydrates as an additional ingredient such as ordinary beverages. Such natural carbohydrates are monosaccharides such as glucose and fructose, disaccharides such as maltose and sucrose, and polysaccharides such as dextrin and cyclodextrin, and sugar alcohols such as xylitol, sorbitol and erythritol. Examples of sweeteners include natural sweeteners such as tau martin and stevia extract, synthetic sweeteners such as saccharin and aspartame, and the like. The proportion of the natural carbohydrate is generally about 0.01 to 0.04 g, preferably about 0.02 to 0.03 g per 100 ml of the composition of the present invention.
상기 외에 본 발명의 가래나무 추출물은 여러 가지 영양제, 비타민, 전해질, 풍미제, 착색제, 펙트산 및 그의 염, 알긴산 및 그의 염, 유기산, 보호성 콜로이드 증점제, pH 조절제, 안정화제, 방부제, 글리세린, 알코올, 탄산음료에 사용되는 탄산화제 등을 함유할 수 있다. 그 밖에 본 발명의 가래나무 추출은 천연 과일 주스, 과일 주스 음료 및 야채 음료의 제조를 위한 과육을 함유할 수 있다. 이러한 성분은 독립적으로 또는 혼합하여 사용할 수 있다. 이러한 첨가제의 비율은 크게 중요하진 않지만 본 발명의 조성물 100 중량부당 0.01 ~ 0.1 중량부의 범위에서 선택되는 것이 일반적이다.
In addition to the above, the extract of the sputum tree of the present invention is various nutrients, vitamins, electrolytes, flavors, coloring agents, pectic acid and salts thereof, alginic acid and salts thereof, organic acids, protective colloidal thickeners, pH regulators, stabilizers, preservatives, glycerin, Alcohol, a carbonation agent used for carbonated drinks, and the like. In addition, the sputum extract of the present invention may contain pulp for the production of natural fruit juices, fruit juice drinks and vegetable drinks. These components may be used independently or in combination. The proportion of such additives is not critical, but is generally selected in the range of 0.01 to 0.1 parts by weight per 100 parts by weight of the composition of the present invention.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 주름개선용 피부 외용제를 제공한다.The present invention also provides a skin external preparation for improving skin wrinkles containing a sputum extract as an active ingredient.
상기 가래나무는 가래나무 가지, 줄기, 뿌리 또는 잎을 이용하는 것이 바람직하고, 가래나무 잎을 이용하는 것이 가장 바람직하나 이에 한정하지 않는다.The sputum tree is preferably using a sputum branch, stem, root or leaf, it is most preferred to use a sputum leaf, but is not limited thereto.
본 발명의 가래나무 추출물은 우수한 세포보호 효과, ROS 억제 효과, MMP-1의 억제 효과 및 COL1A1 보호 효과를 가지고 있어, 피부 주름개선용 피부 외용제로서 유용하게 사용될 수 있다.
Sputum bark extract of the present invention has an excellent cytoprotective effect, ROS inhibitory effect, MMP-1 inhibitory effect and COL1A1 protective effect, it can be usefully used as a skin external preparation for skin wrinkle improvement.
본 발명의 가래나무 추출물을 피부 외용제로 사용하는 경우, 추가로 지방 물질, 유기 용매, 용해제, 농축제 및 겔화제, 연화제, 항산화제, 현탁화제, 안정화제, 발포제(foaming agent), 방향제, 계면활성제, 물, 이온형 또는 비이온형 유화제, 충전제, 금속이온봉쇄제 및 킬레이트화제, 보존제, 비타민, 차단제, 습윤화제, 필수 오일, 염료, 안료, 친수성 또는 친유성 활성제, 지질 소낭 또는 피부용 외용제에 통상적으로 사용되는 임의의 다른 성분과 같은 피부 과학 분야에서 통상적으로 사용되는 보조제를 함유할 수 있다. 또한, 상기 성분들은 피부 과학 분야에서 일반적으로 사용되는 양으로 도입될 수 있다.In the case of using the extract of the sputum tree of the present invention as an external preparation for skin, it is additionally used for fatty substances, organic solvents, solubilizers, thickening and gelling agents, emollients, antioxidants, suspending agents, stabilizers, foaming agents, fragrances, and interfaces. To active agents, water, ionic or nonionic emulsifiers, fillers, metal ion sequestrants and chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic active agents, lipid vesicles or external preparations for the skin It may contain adjuvants commonly used in the field of dermatology, such as any other ingredients commonly used. In addition, the components can be introduced in amounts commonly used in the dermatology field.
상기 피부 외용제에 가래나무 추출물의 투여량은 0.0001 내지 100 ㎎/㎏이고, 바람직하게는 0.001 내지 10 ㎎/㎏이며, 이에 제한되는 것은 아니다. 투여량은 특정 환자의 체중, 연령, 성별, 건강상태, 투여기간, 제거율, 질환의 중증도 등에 따라 변화될 수 있다.
The dosage of the sputum tree extract in the external preparation for skin is 0.0001 to 100 mg / kg, preferably 0.001 to 10 mg / kg, but is not limited thereto. Dosage may vary depending on the weight, age, sex, health status, duration of administration, clearance, severity of the disease, and the like of the particular patient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 노화방지용 화장료 조성물을 제공한다.The present invention also provides a cosmetic composition for preventing skin aging containing the extract of phlegm as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 노화방지용 약학적 조성물을 제공한다.The present invention also provides a pharmaceutical composition for preventing skin aging containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 노화방지용 건강식품용 조성물을 제공한다.In addition, the present invention provides a composition for preventing skin aging health food containing the extract of phlegm as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 노화방지용 피부 외용제를 제공한다.In addition, the present invention provides a skin external preparation for skin aging containing a sputum extract as an active ingredient.
상기 가래나무는 가래나무 가지, 줄기, 뿌리 또는 잎을 이용하는 것이 바람직하고, 가래나무 잎을 이용하는 것이 가장 바람직하나 이에 한정하지 않는다.The sputum tree is preferably using a sputum branch, stem, root or leaf, it is most preferred to use a sputum leaf, but is not limited thereto.
본 발명의 가래나무 추출물은 우수한 세포보호 효과, ROS 억제 효과, MMP-1의 억제 효과 및 COL1A1 보호 효과를 가지고 있어, 피부 노화방지용 화장료 조성물, 약학적 조성물, 건강식품용 조성물 및 피부 외용제로서 유용하게 사용될 수 있다.
Sputum bark extract of the present invention has an excellent cytoprotective effect, ROS inhibitory effect, MMP-1 inhibitory effect and COL1A1 protective effect, useful as skin anti-aging cosmetic composition, pharmaceutical composition, health food composition and skin external preparation Can be used.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 보호용 화장료 조성물을 제공한다.The present invention also provides a cosmetic composition for skin protection containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 보호용 약학적 조성물을 제공한다.The present invention also provides a pharmaceutical composition for protecting the skin containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 보호용 건강식품용 조성물을 제공한다.In addition, the present invention provides a composition for health food for skin protection containing a sputum extract as an active ingredient.
또한, 본 발명은 가래나무 추출물을 유효성분으로 함유하는 피부 보호용 피부 외용제를 제공한다.In addition, the present invention provides a skin external preparation for skin protection containing a sputum extract as an active ingredient.
상기 가래나무는 가래나무 가지, 줄기, 뿌리 또는 잎을 이용하는 것이 바람직하고, 가래나무 잎을 이용하는 것이 가장 바람직하나 이에 한정하지 않는다.The sputum tree is preferably using a sputum branch, stem, root or leaf, it is most preferred to use a sputum leaf, but is not limited thereto.
본 발명의 가래나무 추출물은 우수한 세포보호 효과, ROS 억제 효과, MMP-1의 억제 효과 및 COL1A1 보호 효과를 가지고 있어, 피부 보호용 화장료 조성물, 약학적 조성물, 건강식품용 조성물 및 피부 외용제로서 유용하게 사용될 수 있다.
Sputum bark extract of the present invention has an excellent cytoprotective effect, ROS inhibitory effect, MMP-1 inhibitory effect and COL1A1 protective effect, it can be usefully used as a cosmetic composition, pharmaceutical composition, health food composition and skin external preparation for skin protection Can be.
또한, 본 발명은 가래나무 추출물을 함유하는 색조 화장료 조성물을 제공한다.The present invention also provides a color cosmetic composition containing a sputum extract.
상기 본 발명의 색조 화장료 조성물은 통상적으로 제조되는 모든 제형으로 제조할 수 있으며, 립스틱, 아이라이너, 메이크업 파운데이션, 마스카라, 볼터치 및 아이쉐도우로 이루어진 군으로부터 선택된 어느 하나의 제형에 사용되는 것이 바람직하다.The color cosmetic composition of the present invention can be prepared in all formulations prepared conventionally, it is preferable to be used in any one formulation selected from the group consisting of lipstick, eyeliner, makeup foundation, mascara, ball touch and eye shadow. .
본 발명의 가래나무 추출물은 진한 녹색을 띄고 있으므로, 색조 화장품에 쓰이고 있는 화학성 색소원료(청색 1호 브릴리안트블루 FCF, 청색 2호 인디고 카르민, 녹색 404호 프탈로시아닌 블루)를 대체하는 색조 화장료 조성물에 유용하게 이용할 수 있다.Since the extract of the sputum tree of the present invention has a dark green color, to the color cosmetic composition to replace the chemical pigment raw materials (blue No. 1 Brilliant Blue FCF, blue No. 2 indigo carmine, Green No. 404 phthalocyanine blue) used in color cosmetics It can be usefully used.
본 발명의 가래나무 추출물을 이용한 색조 화장료 조성물에서, 가래나무 추출물의 함량은 전체 화장료 조성물에 대하여 0.0001 내지 50 중량%로 포함될 수 있으며, 더욱 바람직하게는 0.0001 내지 30 중량%으로 포함되는 것이나, 이에 한정하지 않는다. In the color cosmetic composition using a sputum bark extract of the present invention, the content of sputum bark extract may be included in 0.0001 to 50% by weight, more preferably 0.0001 to 30% by weight relative to the total cosmetic composition, but is not limited thereto. I never do that.
이하, 본 발명을 실시예, 실험예 및 제조예에 의해 상세히 설명한다.Hereinafter, the present invention will be described in detail with reference to Examples, Experimental Examples and Preparation Examples.
단, 하기 실시예, 실험예 및 제조예는 본 발명을 예시하는 것일 뿐, 본 발명의 내용이 하기 실시예, 실험예 및 제조예에 의해 한정되는 것은 아니다.
However, the following Examples, Experimental Examples and Preparation Examples are merely illustrative of the present invention, and the content of the present invention is not limited by the following Examples, Experimental Examples and Preparation Examples.
<< 실시예Example 1> 가래나무( 1> phlegm JunglansJunglans mandshuricamandshurica MAXIMMAXIM ) 추출물의 제조) Preparation of extract
가래나무는 강원도 횡성군 가래나무 재배지에서 구입하였다. The phlegm was purchased from the plantation of phlegm in Hoengseong-gun, Gangwon-do.
가래나무의 잎은 70% 에탄올을 시료의 10배 양으로 하여 상온에서 24시간 동안 침출시킨 다음, 감압여과하여(Whatman filter paper #2), 건조 분말화(수득률 5.16%)하여 -20℃에서 보관하였고, 실험시 용시조제하여 사용하였다.
The leaves of the sputum tree were leached at room temperature for 24 hours with 70% ethanol in 10 times the amount of sample, then filtered under reduced pressure (Whatman filter paper # 2), and then dried and powdered (yield 5.16%) and stored at -20 ° C. It was used as a preparation for the experiment.
<< 실험예Experimental Example 1> 가래나무 추출물의 세포 보호 효과 확인 1> Confirmation of cell protective effect of sputum tree extract
상기 <실시예 1>에서 제조된 가래나무 추출물의 H2O2자극에 대한 인간 피부 섬유아세포(Human dermal fibroblasts; HS68)의 보호 효과를 확인하였다.The protective effect of human dermal fibroblasts (HS68) on H 2 O 2 stimulation of the sputum tree extract prepared in <Example 1> was confirmed.
구체적으로, HS68 세포를 플레이트에 0.5 × 104 세포/웰로 씨딩(seeding)하고, 12시간 동안 배양한 후, FBS free DMEM 배지로 50, 100, 200 및 400 ug/ml 농도로 희석한 가래나무 추출물 각각 농도별로 전처리한 다음, 700 ㎛ H2O2를 처리하거나 비처리하여 배양하였다. 이후, 1 mg/mL의 MTT를 처리하고 2시간 후, 세포에 MTT 처리로 인해 생성된 포마잔(formazan)을 DMSO로 녹이고 OD 570nm에서 그 흡광도를 측정하여, H2O2 자극에 대한 HS68 세포의 생존률을 확인하였다.Specifically, HS68 cells were seeded at 0.5 × 10 4 cells / well on a plate, incubated for 12 hours, and then sputum tree extract diluted to 50, 100, 200 and 400 ug / ml concentration with FBS free DMEM medium. After pretreatment for each concentration, the cells were incubated with or without 700 μm H 2 O 2 . Then, 2 hours after treatment with 1 mg / mL of MTT, formazan produced by MTT treatment in cells was dissolved in DMSO, and its absorbance was measured at OD 570 nm to measure HS68 cells for H 2 O 2 stimulation. The survival rate of was confirmed.
상기 얻어진 결과 및 이하 모든 결과에 대한 통계처리는 GraphPad Prism 프로그램을 사용하여 실시하였으며, one-way analysis of vatiance(ANOVA)를 이용하여 평균값의 유의성을 5% 미만의 한계로 조사하였다.Statistical results of the obtained results and all the following results were performed using the GraphPad Prism program, and the significance of the mean value was examined using the one-way analysis of vatiance (ANOVA) with a limit of less than 5%.
그 결과, 도 1 및 도 2에 나타낸 바와 같이, HS68 세포에 각각의 가래나무 추출물을 처리한 경우, 추출물 자체는 세포 생존율에 영향을 미치지 않았다(도 1).As a result, as shown in Figs. 1 and 2, when HS68 cells were treated with each phlegm extract, the extract itself did not affect the cell viability (Fig. 1).
또한, 각각의 가래나무 추출물을 전처리한 경우, H2O2 독성에 대하여 세포 보호 효과를 나타내었다(도 2). 특히 가래나무 추출물 100 ug/ml 처리군에서 가장 좋은 효과를 보였으며, 이 수치는 양성 대조군인 항산화제 NAC(N-아세틸시스테인, N-Acetyl-L-Cysteine) 및 녹차와 비교해본 결과, 유의적인 세포 보호효과를 나타내는 것을 확인하였다(도 2).In addition, when pretreated with each extract of phlegm, it showed a cell protective effect against H 2 O 2 toxicity (Fig. 2). In particular, 100 ug / ml of sputum extract showed the best effect, and this value was significant compared to the antioxidant controls NAC (N-acetylcysteine, N-Acetyl-L-Cysteine) and green tea. It was confirmed that the cell protective effect (Fig. 2).
따라서, 가래나무 추출물 자체는 HS68 세포에 독성을 나타내지 않고, H2O2 독성에 대한 HS68 세포의 보호 효과를 나타냄을 확인하였다.
Therefore, it was confirmed that the extract of phlegm itself does not show toxicity to HS68 cells, but shows a protective effect of HS68 cells against H 2 O 2 toxicity.
<< 실험예Experimental Example 2> 2> HS68HS68 세포에서 가래나무 추출물의 Of phlegm extract in cells ROSROS 과생성And creation 억제 효과 확인 Confirmation of inhibition
HS68 세포에서 H2O2에 의해 과생성된 ROS(Reactive Oxygen Species)에 대한 가래나무 추출물의 효과를 확인하기 위하여, HS68 세포에 50, 100 및 200 ug/mL의 가래나무 추출물과 700 ㎛ H2O2를 처리한 후, 25 uM H2 DCF-DA를 넣고 30분 동안 배양한 다음, 여기(excitation) 480nm, 방출(emission) 530nm에서 형광도를 측정하여 DCF 형광 강도(DCF fluorescence intensity)를 비교하였다.To determine the effect of sputum extract on reactive oxygen species (ROS) overproduced by H 2 O 2 in HS68 cells, 50, 100 and 200 ug / mL sputum extract and 700 μm H 2 on HS68 cells After treatment with O 2 , 25 uM H 2 DCF-DA was added and incubated for 30 minutes, followed by measurement of fluorescence at excitation 480 nm and emission 530 nm to compare DCF fluorescence intensity. It was.
그 결과, 도 3에 나타낸 바와 같이, 가래나무 추출물은 대조군에 비하여 50, 100 및 200 ug/mL의 농도에서 농도의존적으로 H2O2에 의해 과생성된 ROS를 효과적으로 억제할 뿐 아니라, 유의적인 세포 보호 효과를 나타내는 것을 확인하였다(도 3).As a result, as shown in Fig. 3, the extract of the sputum tree effectively inhibits ROS overproduced by H 2 O 2 at concentrations of 50, 100 and 200 ug / mL as compared to the control group, It was confirmed that the cell protective effect (Fig. 3).
따라서, 가래나무 추출물은 인간 피부 섬유아세포(HS68)에서 H2O2에 의해 과생성된 ROS의 억제 효과가 있음을 확인하였다.
Therefore, it was confirmed that sputum extract has an inhibitory effect of ROS overproduced by H 2 O 2 in human skin fibroblasts (HS68).
<< 실험예Experimental Example 3> 3> HS68HS68 세포에서 가래나무 추출물의 Of phlegm extract in cells MMPMMP -1 억제 효과-1 inhibitory effect
가래나무 추출물의 MMP-1(Matrix Metallo Proteinase) 효소 활성에 대한 효과를 확인하기 위하여, HS68 세포를 100 mm 디쉬에 0.3 × 105/디쉬로 씨딩한 후, 12시간 동안 배양한 후, FBS free DMEM 배지에 50, 100 및 200 ug/mL으로 희석한 가래나무 추출물 각각을 농도별로 전처리한 다음, 700 ㎛ H2O2를 처리하거나 비처리하여 배양하였다. 이후, 배지를 수집하여 MMP-1 Kit(Amersham bioscience)를 이용하여 배지 내 분비된 MMP-1의 양을 측정하였다. In order to confirm the effect on the MMP-1 (Matrix Metallo Proteinase) enzyme activity of the extract of sputum, HS68 cells were seeded in 0.3 × 10 5 / dish in 100 mm dish, incubated for 12 hours, and then FBS free DMEM Each of the extracts of sputum tree diluted to 50, 100 and 200 ug / mL in the medium was pre-treated by concentration, and then cultured by treating or untreated with 700 ㎛ H 2 O 2 . Thereafter, the medium was collected and the amount of MMP-1 secreted in the medium was measured using the MMP-1 Kit (Amersham bioscience).
그 결과, 도 4에 나타낸 바와 같이 가래나무 추출물은 H2O2에 의해 증가한 MMP-1에 대하여 농도 의존적인 MMP-1 효소 활성을 억제 효과를 확인하였다(도 4).As a result, as shown in FIG. 4, the extract of phlegm confirmed the inhibitory effect of concentration-dependent MMP-1 enzyme activity against MMP-1 increased by H 2 O 2 (FIG. 4).
따라서, 본 발명의 가래나무 추출물이 콜라겐 분해 효소인 MMP-1을 억제함으로써, 주름 생성을 억제하는 효과가 있음을 확인하였다.
Therefore, it was confirmed that the phlegm extract of the present invention has an effect of suppressing wrinkle formation by inhibiting MMP-1, which is a collagen degrading enzyme.
<< 실험예Experimental Example 4> 4> HS68HS68 세포에서 가래나무 추출물의 Of phlegm extract in cells COL1A1COL1A1 (( propro -- collagencollagen typetype 1) 보호 효과 확인 1) Check the protective effect
가래나무 추출물의 피부세포에서 콜라겐을 생합성하는 COL1A1에 대한 효과를 확인하기 위하여, 상기 <실시예 3>과 동일한 방법으로, HS68 세포에 가래나무 추출물을 각각 50, 100 및 200 ug/mL 전처리한 다음, 700 ㎛ H2O2를 처리하거나 비처리하여 배양하였다. 그런 다음 반응이 끝난 세포를 수집한 후, 세포의 전체 단백질을 이용하여 COL1A1 단백질 발현을 측정하였다.In order to confirm the effect of COL1A1 on collagen biosynthesis in skin cells of the sputum tree extract, in the same manner as in <Example 3>, 50, 100 and 200 ug / mL of the sputum tree extract to HS68 cells, respectively, Incubated with or without treatment, 700 μm H 2 O 2 . Then, after the reaction was completed, the total protein of the cells was used to measure COL1A1 protein expression.
구체적으로, 수집한 세포를 단백질 정량 후 전기 영동장치를 이용하여 15% SDS-아크릴아미드 겔(SDS-acrylamide gel)에 로딩하고 멤브레인(membrane)에 이동시킨 후 1차 항체로 COL1A1(Abnova)(1:500), β-actin(Santa cruz biotechnology) (1:2000), 2차 항체로 anti-rabbit/mouse HRP-confugated(Santa cruz biotechnology)(1:2000)를 각각 반응시켰다. 각 과정 마다 멤브레인을 TBST로 15분간 4번씩 세척하였다. 항체 반응을 마친 멤브레인은 ECL detection kit(Animal genetics)를 이용하여 LAS-4000 mini system(Fujifilm Corporation, Tokyo, 일본)을 통해 노출 되도록 하였다.Specifically, the collected cells were loaded onto 15% SDS-acrylamide gel using an electrophoresis device after protein quantification, and transferred to a membrane, and then COL1A1 (Abnova) (1) as a primary antibody. : 500), β-actin (Santa cruz biotechnology) (1: 2000), and secondary antibodies were reacted with anti-rabbit / mouse HRP-confugated (Santa cruz biotechnology) (1: 2000). Membranes were washed four times for 15 min with TBST for each procedure. After the antibody reaction, the membrane was exposed through the LAS-4000 mini system (Fujifilm Corporation, Tokyo, Japan) using an ECL detection kit (Animal genetics).
그 결과, 도 5에 나타낸 바와 같이 가래나무 추출물은 H2O2에 의해 감소한 COL1A1 대하여 농도 의존적인 COL1A1 단백질 발현 증가 효과를 나타내는 것을 확인하였다(도 5).As a result, as shown in Figure 5, the extract of phlegm showed a concentration-dependent increase in COL1A1 protein expression with respect to COL1A1 reduced by H 2 O 2 (Fig. 5).
따라서, 본 발명의 가래나무 추출물이 콜라겐을 생합성하는 COL1A1을 보호함으로써, 주름 생성을 억제하는 효과가 있음을 확인하였다.
Therefore, it was confirmed that the sputum bark extract of the present invention has an effect of suppressing wrinkle formation by protecting COL1A1 that biosynthesizes collagen.
<< 제조예Manufacturing example 1> 가래나무 추출물을 유효성분으로 함유하는 1> Containing phlegm extract as an active ingredient 화장료의Cosmetic 제조 Produce
<1-1> 유연 화장수의 제조<1-1> Manufacture of flexible lotion
상기 <실시예 1>의 가래나무 추출물을 유효성분으로 함유하는 유연 화장수는 하기 [표 1]과 같이 제조하였다.
The flexible lotion containing the sputum tree extract of <Example 1> as an active ingredient was prepared as shown in [Table 1].
<1-2> 영양 크림의 제조<1-2> Preparation of nourishing cream
상기 <실시예 1>의 가래나무 추출물을 유효성분으로 함유한 영양크림은 하기 [표 2]의 조성과 같이 제조하였다.
The nourishing cream containing the extract of the phlegm of <Example 1> as an active ingredient was prepared as shown in [Table 2].
<< 제조예Manufacturing example 2> 가래나무 추출물을 유효성분으로 함유하는 약제의 제조 2> Preparation of Pharmaceuticals Containing Sputum Tree Extract as Active Ingredient
<2-1> 시럽제의 제조<2-1> Preparation of Syrup
상기 <실시예 1>의 가래나무 추출물을 유효성분으로 함유하는 시럽제는 하기 [표 3]의 조성과 같이 제조하였다.
Syrup containing the sputum extract of <Example 1> as an active ingredient was prepared as in the composition of the following [Table 3].
<2-2> 정제의 제조<2-2> Preparation of Tablet
상기 <실시예 1>의 가래나무 추출물을 유효성분으로 함유하는 정제는 하기 [표 4]의 조성과 같이 제조하였다.
Tablets containing the phlegm extract of <Example 1> as an active ingredient was prepared as in the composition of the following [Table 4].
상기 <실시예 1>의 가래나무 추출물 250 중량부, 락토오스 175.9 중량부, 감자전분 180 중량부 및 콜로이드성 규산 32 중량부와 혼합하였다. 상기 혼합물에 10% 젤라틴 용액을 첨가시킨 후, 분쇄하여 14 매쉬체를 통과시켰다. 이것을 건조하고 여기에 감자전분 160 중량부, 활석 50 중량부 및 스테아린산 마그네슘 5 중량부를 첨가하여 얻은 혼합물을 정제로 제조하였다.
250 parts by weight of the sputum tree extract of <Example 1>, 175.9 parts by weight of lactose, 180 parts by weight of potato starch and 32 parts by weight of colloidal silicic acid. 10% gelatin solution was added to the mixture, followed by pulverization and passed through a 14-mesh sieve. This was dried, to which 160 parts by weight of potato powder, 50 parts by weight of talc and 5 parts by weight of magnesium stearate were added, and the resulting mixture was purified.
<< 제조예Manufacturing example 3> 가래나무 추출물을 유효성분으로 함유하는 건강식품의 제조 3> Preparation of Health Foods Containing Sputum Tree Extract as Active Ingredient
상기 <실시예 1>의 가래나무 추출물을 유효성분으로 함유하는 식품들을 다음과 같이 제조하였다.Foods containing the phlegm extract of <Example 1> as an active ingredient were prepared as follows.
<3-1> 밀가루 식품의 제조<3-1> Production of flour food
상기 <실시예 1>의 가래나무 추출물 0.5~5.0 중량부를 밀가루에 첨가하고, 이 혼합물을 이용하여 빵, 케이크, 쿠키, 크래커 및 면류를 제조하였다.
0.5-5.0 parts by weight of the sputum tree extract of <Example 1> was added to the flour, and bread, cake, cookies, crackers and noodles were prepared using this mixture.
<3-2> <3-2> 스프soup 및 육즙( And juicy ( graviesgravies )의 제조Manufacturing
상기 <실시예 1>의 가래나무 추출물 0.1~5.0 중량부를 스프 및 육즙에 첨가하여 건강 증진용 육가공 제품, 면류의 수프 및 육즙을 제조하였다.
0.1-5.0 parts by weight of the extract of phlegm of <Example 1> was added to soups and broth to prepare meat products for health promotion, soup of noodles and broth.
<3-3> 그라운드 <3-3> Ground 비프(ground beef)의Beef 제조 Produce
상기 <실시예 1>의 가래나무 추출물 10 중량부를 그라운드 비프에 첨가하여 건강 증진용 그라운드 비프를 제조하였다.
10 parts by weight of the extract of phlegm of <Example 1> was added to the ground beef to prepare a ground beef for health promotion.
<3-4> 유제품(<3-4> Dairy products ( dairydairy productsproducts )의 제조Manufacturing
상기 <실시예 1>의 가래나무 추출물 5~10 중량부를 우유에 첨가하고, 상기 우유를 이용하여 버터 및 아이스크림과 같은 다양한 유제품을 제조하였다.
5 to 10 parts by weight of the sputum tree extract of <Example 1> was added to milk, and various dairy products such as butter and ice cream were prepared using the milk.
<3-5> <3-5> 선식의Solar 제조 Produce
현미, 보리, 찹쌀, 율무를 공지의 방법으로 알파화시켜 건조시킨 것을 배전한 후 분쇄기로 입도 60 메쉬의 분말로 제조하였다.Brown rice, barley, glutinous rice, and yulmu were dried by a known method and dried, and the mixture was granulated to a powder having a particle size of 60 mesh.
검정콩, 검정깨, 들깨도 공지의 방법으로 쪄서 건조시킨 것을 배전한 후 분쇄기로 입도 60 메쉬의 분말로 제조하였다.Black soybeans, black sesame seeds, and perilla seeds were steamed and dried by a conventional method, and then they were prepared into powder having a particle size of 60 mesh by a pulverizer.
상기 <실시예 1>의 가래나무 추출물을 진공 농축기에서 감압농축하고, 분무, 열풍건조기로 건조하여 얻은 건조물을 분쇄기로 입도 60 메쉬로 분쇄하여 건조분말을 얻었다.The sputum tree extract of <Example 1> was concentrated under reduced pressure in a vacuum concentrator, and the dried product obtained by drying with a spray and a hot air dryer was pulverized to a particle size of 60 mesh with a grinder to obtain a dry powder.
상기에서 제조한 곡물류, 종실류 및 실시예 <1-3>의 추출물을 다음의 비율로 배합하여 제조하였다.The grains, seeds, and extracts of Example <1-3> prepared above were prepared by combining the following ratios.
곡물류(현미 30 중량부, 율무 15 중량부, 보리 20 중량부),(30 parts by weight of brown rice, 15 parts by weight of yulmu, 20 parts by weight of barley)
종실류(들깨 7 중량부, 검정콩 8 중량부, 검정깨 7 중량부),Seeds (7 parts by weight of perilla, 8 parts by weight of black beans, 7 parts by weight of black sesame seeds)
<실시예 1>의 가래나무 추출물(3 중량부),Sputum tree extract of Example 1 (3 parts by weight),
영지(0.5 중량부),(0.5 part by weight),
지황(0.5 중량부)
(0.5 parts by weight)
<< 제조예Manufacturing example 4> 가래나무 추출물을 유효성분으로 함유하는 음료의 제조 4> Preparation of beverage containing sputum tree extract as active ingredient
<4-1> <4-1> 건강음료의Health drink 제조 Produce
액상과당(0.5%), 올리고당(2%), 설탕(2%), 식염(0.5%), 물(75%)과 같은 부재료와 본 발명의 <실시예 1>의 가래나무 추출물 5 g을 균질하게 배합하여 순간 살균을 한 후 이를 유리병, 패트병 등 소포장 용기에 포장하여 제조하였다.
Homogeneous components such as liquid fructose (0.5%), oligosaccharide (2%), sugar (2%), salt (0.5%), water (75%) and 5 g of the sputum tree extract of <Example 1> of the present invention After sterilization and instant sterilization, it was prepared by packaging in small packaging containers such as glass bottles and plastic bottles.
<4-2> 야채 주스의 제조<4-2> Production of vegetable juice
상기 <실시예 1>의 가래나무 추출물 5 g을 토마토 또는 당근 주스 1,000 ㎖에 가하여 야채 주스를 제조하였다.
5 g of the sputum tree extract of <Example 1> was added to 1,000 ml of tomato or carrot juice to prepare vegetable juice.
<4-3> 과일 주스의 제조<4-3> Preparation of fruit juice
상기 <실시예 1>의 가래나무 추출물 1 g을 사과 또는 포도 주스 1,000 ㎖ 에 가하여 과일 주스를 제조하였다.
1 g of the sputum tree extract of <Example 1> was added to 1,000 ml of apple or grape juice to prepare a fruit juice.
Claims (12)
Sputum Tree Extract ( Junglans Cosmetic composition for improving skin wrinkles containing mandshurica MAXIM) as an active ingredient.
The cosmetic composition for improving skin wrinkles according to claim 1, wherein the sputum tree extract is extracted with water, C 1 to C 4 lower alcohols, or a mixed solvent thereof.
The cosmetic composition for improving skin wrinkles according to claim 2, wherein the lower alcohol is ethanol or methanol.
The cosmetic composition for improving skin wrinkles according to claim 1, wherein the sputum extract is a sputum leaf extract.
Pharmaceutical composition for improving skin wrinkles containing sputum tree extract as an active ingredient.
Health food composition for improving skin wrinkles containing sputum extract as an active ingredient.
Skin external preparation for improving skin wrinkles containing sputum tree extract as an active ingredient.
Cosmetic composition for skin aging and skin protection containing sputum tree extract as an active ingredient.
A pharmaceutical composition for preventing skin aging and skin protection containing the extract of sputum as an active ingredient.
Health food composition for skin aging and skin protection containing sputum tree extract as an active ingredient.
Skin external preparation for skin aging and skin protection containing sputum extract as an active ingredient.
Color cosmetic composition containing sputum extract.
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| WO2018169228A1 (en) * | 2017-03-15 | 2018-09-20 | 연세대학교 산학협력단 | Composition for preventing or treating pigmentation disorders caused by hyperpigmentation |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| KR101828584B1 (en) * | 2016-02-12 | 2018-03-29 | 순천향대학교 산학협력단 | Composition containing complex extracts including Veratrum nigrum var. ussuriense, Juglans mandshurica, Rodgersia podophylla and Chaenomeles sinensis with antioxidant activity or whitening |
| KR101866372B1 (en) | 2016-10-06 | 2018-06-11 | 연세대학교산학협력단 | Pharmaceutical composition for preventing or treating obesity, comprising a compounds derived from an extract or fraction or Juglans mandshurica |
| KR102047461B1 (en) * | 2018-02-23 | 2019-11-21 | 주식회사 엘지생활건강 | Composition for improving wrinkle comprising Symplocarpus foetidus extract or fractions thereof |
| WO2020209690A1 (en) | 2019-04-12 | 2020-10-15 | 가천대학교 산학협력단 | Pharmaceutical composition containing wild-walnut extract, fraction thereof, or physiologically active substance derived therefrom as active ingredient, and having effects of inhibiting production of advanced glycation end products and decomposing advanced glycation end products |
| KR20220052815A (en) | 2020-10-08 | 2022-04-28 | 재단법인춘천바이오산업진흥원 | Composition for prevention, treatment or improvement of obesity with the extract of Juglans mandshurica |
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| WO2018169228A1 (en) * | 2017-03-15 | 2018-09-20 | 연세대학교 산학협력단 | Composition for preventing or treating pigmentation disorders caused by hyperpigmentation |
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