TWI434696B - 用於下調促發炎指標的組成物 - Google Patents
用於下調促發炎指標的組成物 Download PDFInfo
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- TWI434696B TWI434696B TW099129450A TW99129450A TWI434696B TW I434696 B TWI434696 B TW I434696B TW 099129450 A TW099129450 A TW 099129450A TW 99129450 A TW99129450 A TW 99129450A TW I434696 B TWI434696 B TW I434696B
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- polysaccharide
- acid
- tnf
- inflammatory
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Description
本發明關於一種包含得自乳香屬植物的乳香脂酸部分及多醣部分的組成物。該組成物在增強的生物活性,特別是下調促發炎指標上展現增效性。
本發明亦關於多醣部分單獨或與乳香脂酸部分組合時在抑制PGE2上的用途。
若沒有免疫系統的精密調節即不可能存活。促發炎細胞激素(pro-inflammatory cytokines)的生產為發育、組織再生、癒合、外傷或感染期間協調免疫與代謝回應的關鍵生理程序,並保護身體免於出血、缺血、癌症與敗血症。促發炎細胞激素,例如介白素(ILs,Interleukins)、腫瘤壞死因子-α(TNF-α)在受控下生產可觸發有利的發炎反應,即促進局部凝血以局限感染與組織損壞(參見Ulloa及Tracey,2005)。但是,此等細胞激素的無限制生產會比原始的傷害要更加危險,且其為人類發病率與死亡率的主要原因之一。此程序最嚴重的例子中之一個為「嚴重的敗血症」(severe sepsis),此為加護病房中死亡的首要原因,且為已開發社會中死亡的主要原因中之一個(參見Martin等人,2003)。嚴重敗血症的特徵在於大量地生產促發炎細胞激素,造成全身性發炎、心血管功能障礙,及致命的多發性器官衰竭(參見Van der Poll及Lowry,1995;Hotchkiss及Karl,2003;Rice及Bernard,2005)。此效應可由一些研究例示說明,此等研究指出中和促發炎細胞激素(單株抗-TNF抗體及IL-1受體拮抗劑)已被證明可成功地應用於發炎症狀,諸如類風濕性關節炎、克隆氏症(Crohn’s disease)、僵直性脊椎炎及乾癬(見於Feldmann,2002;Ulloa及Tracey,2005;Rutgeerts等人,2006;Ulloa及Messmer,2006)。
除了細胞激素之外,其它的媒介物,像是組織胺、前列腺素、白三烯酸(leukotrienes)、緩激肽(bradykinin)等,亦皆在發炎反應中扮演重要角色。因此,此等可做為指標,並可用於診斷疾病症狀,尤其是此等指標以升高濃度存在的狀況。因此必須調控這些指標以精密控制免疫系統。
齒葉乳香樹(印度乳香樹)(Boswellia serrata)的樹膠脂(N.O. Burseraceae)被稱為「Dhup」,印度乳香(Indian Frankincense或Indian olibanum)用於宗教儀式及香料應用已有很長的歷史。印度乳香在健康上的應用,在阿育吠陀傳統草藥(Ayurvedic)中久為人知,而在過去三十年中亦已被西方世界注意到,而造成樹膠脂滲出物之標準化萃取物的擴大並用。這種萃取物被用作飲食補充劑及化粧品中的成分以維護老年人的健康。在飲食補充劑中最常見的應用為關節健康維護產品,以維護正常的關節功能與活動性。
新近的科學證據越來越支持齒葉乳香樹的增進健康效果。基本上,已報告齒葉乳香樹的樹膠油性樹脂滲出物含有倍半帖類精油(sesquiterpenoid essential oils)(8-12%/w/w),多醣類(45-60% w/w),及較高碳的類萜素(terpenoids)(25-35% w/w)。在樹膠脂的萃取物中之生物指標成分為一群五環三帖化合物(group of pentacyclic triterpene),其被稱為乳香脂酸(boswellic acids)。
乳香脂酸已顯示可抑制酵素5-脂氧合酶(lipoxygenase),該酵素可催化從花生四烯酸(archidonic acid)形成促發炎的白三烯。除了該機制之外,乳香脂酸亦可降低酵素人類白血球彈性酶(HLE,Human Leukocyte Elastase)的活性。此雙重作用為乳香脂酸所獨有(參見Safayhi,H等人,1997)。因為在一些基於發炎及過敏的人類疾病中,白三烯的形成及HLE的釋出皆會因嗜中性球的刺激而同時增加,一般相信所報告的兩種促發炎酵素被乳香脂酸封阻以及其對於補體蛋白質之有利效果及肥大細胞(mast cell)安定化活性可能為乳香萃取物之增進健康效果的基本原理,此被記載於多個臨床前研究及臨床研究。齒葉乳香樹的樹膠脂的萃取物通常在本質上為樹脂狀,且該生物指標乳香脂酸(親脂性化合物)不溶於水。
美國專利US 2003/0186932申請案揭示一種由齒葉乳香樹之樹膠脂滲出物所分離出來的水溶性生物活性部分。其進一步揭示該部分與乳香脂酸以相等比例(1:1)組合後可顯示出加成效果(additive effect),且對於抗關節炎活性無增效效果(synergistic effect)。
美國專利第7582314號記載一種藉由投與包含乳香脂酸與硒元素的組成物來治療牛皮癬病人的方法。
美國專利20080275117申請案記載一種藉由使用包含大於65%之乳香脂酸及/或其醋酸鹽的組成物來治療關節炎的方法。該組成物進一步包含多醣類以及醋酸正-辛酯,因香酚(incensole)、因香酚醋酸酯(incensole acetate)、沉香醇(linalool)、龍腦(borneol)、莰烯(camphene)、欖香烯(elemene)、石竹烯(caryophyllene)、因香酚氧化物、因香酚氧化物醋酸酯及其組合。
本發明涵蓋包含得自乳香屬植物的乳香脂酸部分及多醣部分的組成物,該組成物在下調/抑制促發炎指標上展現增強作用。
本發明係關於一種包含得自乳香屬植物的乳香脂酸部分及多醣部分的組成物,其用於下調促發炎指標,諸如TNF-α、IL-1β、一氧化氮、IFN-γ及LTB4。乳香脂酸部分的濃度為大約60%,而多醣部分的濃度為大約40%。
包含乳香脂酸部分及多醣部分的組成物,相較於個別部分,可增強其活性。
本發明另外關於單獨的多醣部分或其與乳香脂酸部分之組合在抑制PGE2上的用途。
本發明係關於一種增效性組成物,其包含濃度約60%的乳香脂酸部分及濃度約40%的多醣部分,視需要並可包含醫藥上可接受的賦形劑。
在本發明之另一具體實施例中,該乳香脂酸部分與該多醣部分係得自乳香屬植物。
在本發明之又另一具體實施例中,該乳香脂酸部分包含β-乳香脂酸、乙醯基-β-乳香脂酸、11-酮基-β-乳香脂酸及乙醯基-11-酮基-β-乳香脂酸。
在本發明之又另一具體實施例中,該多醣部分包含半乳糖、阿拉伯糖(arabinose)、D-葡萄糖醛酸(glucuronic acid)及4-O-甲基-葡糖醛酸阿拉伯-半乳聚糖(4-O-methyl-glucurono-arabino-galactan)。
在本發明之又另一具體實施例中,該醫藥上可接受的賦形劑係選自包含抗黏著劑、黏合劑、包衣劑、崩散劑、填充劑及稀釋劑、調味劑、著色劑、助滑劑、潤滑劑、保存劑、吸附劑、增甜劑及其組合的群組。
在本發明之又另一具體實施例中,將該組成物調配成選自包含液劑(liquid)、含片(troche)、含錠(lozenges)、粉劑(powder)、顆粒劑(granule)、膠囊劑(capsule)、錠劑(tablet)、貼片(patch)、凝膠劑(gel)、乳劑(emulsion)、乳膏劑(cream)、洗劑(lotion)、潔牙劑(dentrifice)、滴劑(drop)、懸浮液(suspension)、糖漿(syrup)、酏劑(elixir)、植物製劑(phyotceuticals)及食療劑(neutraceuticals)之群組中的劑型。
本發明亦關於一種製備增效性組成物的方法,該組成物包含濃度約60%的乳香脂酸部分及濃度約40%的多醣類部分,視需要並可包含醫藥上可接受的賦形劑,該方法包含以下步驟:由乳香屬植物取得乳香脂酸部分及多醣部分;將濃度約60%的乳香脂酸部分與濃度約40%的多醣部分,視需要連同醫藥上可接受的賦形劑加以組合,得到該組成物。
在本發明之又另一具體實施例中,該乳香脂酸部分包含β-乳香脂酸、乙醯基-β-乳香脂酸、11-酮基-β-乳香脂酸及乙醯基-11-酮基-β-乳香脂酸。
在本發明之又另一具體實施例中,該多醣部分包含半乳糖、阿拉伯糖、D-葡萄糖醛酸及4-O-甲基-葡萄糖醛酸阿拉伯-半乳聚糖。
本發明關於一種用於下調/抑制促發炎指標的方法,該方法包含將一組成物投與至需要其之對象的步驟,該組成物包含濃度約60%之乳香脂酸部分及濃度約40%之多醣部分,視需要並可包含醫藥上可接受的賦形劑。
在本發明之又另一具體實施例中,該乳香脂酸部分及該多醣類部分係得自乳香屬植物。
在本發明之另一具體實施例中,該對象為包括人類在內的動物。
在本發明之另一具體實施例中,該等促發炎指標選自包含TNF-α、IL-β、IFN-γ、一氧化氮及LTB4的群組。
本發明關於一種下調/抑制PGE2的方法,該方法包含將一組成物投與至需要其之對象的步驟,該組成物包含單獨的多醣部分,該組成物亦可包含多醣部分與乳香脂酸部分之組合,該組成物視需要並可包含醫藥上可接受的賦形劑。
在本發明之又另一具體實施例中,該乳香脂酸部分與該多醣類部分係得自乳香屬類植物。
在本發明之又另一具體實施例中,該對象為包括人類在內的動物。
本發明亦關於一種飲食補充劑,其含有如上所述的單獨或組合的各組成物。
本發明代表現有習知乳香萃取物的改良,其可提供製造商水溶性較高且關節健康維護潛力提高的版本。該組成物提供該等活性成分獨特的釋放模式。除了乳香脂酸(乳香脂萃取物的活化方法如一般標準化)之外,來自齒葉乳香樹之樹膠脂的多醣部分亦具有生物活性且為水溶性。如在活體及試管中的研究所顯示,該多醣部分增強該萃取物中乳香脂酸的增進健康角色,且增強程度超過僅是加成作用時的預期值。
包含得自乳香屬植物的乳香脂酸部分與多醣部分的組成物顯示可下調促發炎指標。該組成物,相較於該組成物的個別成分,在下調促發炎細胞激素或媒介物方面顯示增強的活性,因此其本質上為增效性。乳香屬植物的多醣部分為水溶性活性物質且藉此增加乳香脂酸的溶解性(第十三圖),降低乳香脂酸在較高濃度時的毒性,並允許抗發炎作用的維持。
乳香樹膠係用乙醇萃取,且該乙醇萃取物用酸-鹼處理,接著水洗,得到乳香脂酸部分。在該製程中所取得的己烷殘留物(油部分)被丟棄。將乳香樹膠用乙醇萃取後所殘留的殘渣用蒸餾水萃取,並用酒精沉澱,得到多醣部分。將該乳香脂酸部分與該多醣部分分別以約60%與約40%的濃度來組合,以得到本發明的組成物(第十四圖)。
該乳香脂酸部分包含β-乳香脂酸、乙醯基-β-乳香脂酸、11-酮基-β-乳香脂酸及乙醯基-11-酮基-β-乳香脂酸。該多醣部分包含半乳糖、阿拉伯糖、D-葡萄糖醛酸及4-O-甲基-葡萄糖醛酸阿拉伯-半乳聚糖。
該組成物視需要可包含醫藥上可接受的賦形劑,該賦形劑係選自包含抗黏著劑、黏合劑、包衣劑、崩散劑、填充劑及稀釋劑、調味劑、著色劑、助滑劑、潤滑劑、保存劑、吸附劑、增甜劑及其組合的群組。
本發明的組成物係調配成選自包含液劑、含片、含錠、粉劑、顆粒劑、膠囊劑、錠劑、貼片、凝膠劑、乳劑、乳膏劑、洗劑、潔牙劑、滴劑、懸浮液、糖漿、酏劑、植物製劑及食療劑之群組中的劑型。
測試該組成物被測試以了解其在抑制/下調/降低促發炎指標諸如TNF-α、IL-β、一氧化氮、IFN-γ、PGE2及LTB4的程度的潛力。
TNF-α
(腫瘤壞死因子α)為一種涉及全身性發炎並會刺激急性期反應的細胞激素。TNF生產的調控牽涉到多種人類疾病,以及癌症。其在LPS引發的敗血症的發病上扮演重要的角色。
IL-1β
為最強效的促發炎細胞激素中之一個,其同時涉及生理免疫反應及多種免疫病理性失調的進展。檢查IL-1β水平(level)在監測及診斷多種疾病(包括發炎性疾病、免疫性疾病及骨骼疾病)上有用。
一氧化氮
:適當程度的一氧化氮生產在保護器官諸如肝臟免於缺血傷害上頗為重要。但是,持續維持NO產量將造成直接的組織毒性,並為敗血症相關的血管衰竭(vascular collapse)的致成因素。NO之長期表現度與多種癌(carcinoma)及發炎性病症(包括青少年糖尿病、多發性硬化症、關節炎及潰瘍性結腸炎)有關。
藉由下調/降低該等細胞激素或其他媒介物,該組成物在處置多種顯示該等細胞激素水平提高的疾病/失調諸如關節炎、潰瘍性結腸炎、發炎性大腸症候群(IBD,Inflammatory bowel syndrome)、氣喘(呼吸系統失調)等上具有潛在用途。
本發明藉由下述實施例之助而進一步闡明。但是這些實施例不應被視為限制本發明之範圍。
實施例1:生物活性評估
急性安全性研究:
在小鼠中進行急性口部毒性研究,該研究遵循經濟合作暨發展組織(OECD)指南第423號[Organization for Economic Cooperation and Development,化學品測試用OFCD指南,指南423,急性口部毒性-急性毒性分類法,於1996年3月22日採用]。於最初24小時期間定期地同時個別觀察此等動物,尤其在最初4小時期間內要特別注意,之後每天觀察,總共14天。在此等受試動物中,經口投與至每群雌鼠單劑的2000 mg/kg(口服)組成物未顯示總體行為(gross general behavior)有任何變化。亦評估單劑的5000 mg/kg(口服)。與實驗動物的載劑對照群相較下,在此高口服劑量下未觀察到死亡或任何正常行為的變化。
試管內研究:
實施例1:試管內鼠嗜中性球中的細胞內TNF-α評估值:
受試材料接受試管內研究,即進行流式細胞研究以判定乳香脂酸部分、多醣部分及本發明組成物於多種劑量下對於鼠嗜中性球中的細胞內TNF-α細胞激素表現度的影響,其中該鼠嗜中性球係藉由histopaque梯度自全血中分離出。
將細胞用LPS刺激,並在CO2
培養器中與梯度濃度(μg/ml)的受試材料一起培育3小時。將透化溶液(permeabilising solution)加入至該等細胞中,然後將此等培育10分鐘。然後將此等細胞用結合的抗-鼠TNF-α單株抗體標記,並在黑暗中再培育30分鐘。在用磷酸鹽緩衝的食鹽水清洗之後,在BD-CantoII流式細胞儀(來自美國加州的Beckton-Dickinson Biosciences)上直接取得樣品。將螢光激發劑設定為該圈選的嗜中性球群體(10,000事件)的FL1參數,並使用Cell Quest Pro軟體執行螢光補償、數據分析及數據呈現。[Clara,B.,R. C. Arancha,G. M. Andre'
s,P. Atanasio,A. Julia及O. Alberto. 2003所發表的「使用直接免疫螢光表面薄膜染色來偵測TNF-α-分泌細胞的新方法」(J. Immuno. Methods 264:77-78)][Khurshid A. Bhat,Bhahwal A. Shah,Kuldeep K. Gupta,Anjali Pandey,Sarang Bani,Subhash C. Taneja所發表的「可作為人類嗜中性球中TNF-α細胞激素表現度之抑制劑的松醇(pinitol)半合成類似物」(Bioorganic & Medicinal Chemistry Letters 19 2009,1939-1943)]。從由表1、2 & 3(流式細胞儀研究)所提供的結果,可清楚看出與單獨的乳香脂酸部分及多醣部分相較,該組成物對於單離自鼠的嗜中性球回應LPS刺激劑而引起的TNF-α細胞激素分泌,展現最大的抑制作用。在試管中用濃度為25、50、100、200、400及800 μg/ml的乳香脂酸部分、多醣部分及該組成物處理的嗜中性球,於200 μg/ml的劑量水平下分別顯示30.52%、29.31%及59.83%的TNF-α抑制度。從該數據可清楚證明該組成物,與個別部分相較,在相同劑量水平下,在抑制TNF-α上顯示增強的活性。
表1:得自乳香屬植物的乳香脂酸部分於多種劑量下對於鼠嗜中性球的細胞內TNF-α表現度的影響
觀察-3的數目;↓-在鼠嗜中性球中細胞內TNF-α表現度下降;
p值*<0.01;**<0.001;+:細胞死亡。
表2:得自乳香屬植物的多醣部分於多種劑量下對於鼠嗜中性球的細胞內TNF-α表現度的影響
觀察-3的數目;↓-在鼠嗜中性球中細胞內TNF-α表現度下降;
p值*<0.01;**<0.001。
表3:本發明組成物於多種劑量下對於鼠嗜中性球的細胞內TNF-α表現度的影響
觀察-3的數目;↓-在鼠嗜中性球中細胞內TNF-α表現度下降;
p值*<0.01;**<0.001。
活體內硏究:
實施例2:在活體內來自被治療老鼠的血清中的細胞外TNF-α、IL-1β及一氧化氮(NO,nitric oxide)的估計值:
將年齡6-8星期的BALB/c雄鼠保持在22±2℃及12/12小時光亮/黑暗循環的環境中。根據Brieva等人在2001年所提出的方法[參見Brieva A,Guerrero A,Alonso-Lebrero J L及Pivel JP. 2001所發表的「印姆諾芬(Inmunoferon),一種天然的複合醣體,可抑制LPS引發的TNF-α生產及發炎反應」(International Immunopharmacology 1.1979-1987)],此等小鼠接受100、200、400 mg/kg的不同受試物質(w/v)即乳香脂酸部分、多醣部分及本發明組成物的口部治療,總共6天,接著靜脈注射1 mg/kg的LPS。每群使用6隻小鼠,且實驗以同樣方式進行三次。於注射LPS之後90分鐘,藉由市售ELISA套組(R&D Systems)評估來自每一實驗群被治療小鼠的血清中的TNF-α、IL-1β及一氧化氮的產量。使用30 mg/kg的諾普利(Rolipram)作為標準藥物。血清採集及測量顯示TNF-α、IL-1β及NO的血清濃度顯著降低,此暗示就乳香脂酸部分、多醣部分及組成物而言,在活體內控制發炎反應的效力在廣範圍內與物種無關(species-independent)。同時,這些數據暗示在受到LPS刺激的小鼠中,在回應血液中LPS濃度增加方面,該組成物的調控角色較使用單獨的乳香脂酸部分及多醣部分時所觀察到者更為顯著,其不僅針對TNF-α的生產量,而且藉由降低的IL-1β(另一促發炎細胞激素)及NO(第一圖到第六圖)而進一步確證。使用劑量水平為30 mg/kg的諾普利(Rolipram)作為標準藥物,來觀察該實驗設計的真實度(authenicity)及重現度。
為了顯示出該組成物可在生病狀況下發揮作用,進行以下的研究。為此研究所選擇的生病狀況為關節炎。
實施例3:佐藥引發的進展中發炎性關節炎:
在本研究中使用年齡為12-14星期,體重為140-160g的Wistar老鼠,每群6隻。將所有動物保持在22±2℃塑膠籠中,採用12小時的光亮/黑暗循環,並可自由取用食物丸及飲水。在該實驗期間口服受試物質,一天一次。在所有的實驗中,設立一對照群(投與載體),同時其他群接受標準藥物乙醯基水楊酸(ASA,Acetylsalicylic acid)(每天一次)之群,以供比較及證明該試驗的真實度/可信賴度。整個研究係在動物倫理委員會(Institutional Animal Ethics Committee)許可之下進行,且在實驗過程中所使用的所有動物皆接受人道照顧。計算每群的平均值及平均值的標準差(S.E.,Standard error),其結果以相較於對照群的抑制百分比來表示。採用學生t-測試決定統計學上的顯著性(significance)。
佐藥關節炎係由蹠下注射0.05 ml新鮮製備的經蒸氣殺死結核菌懸浮於液體石蠟的懸浮液(5.0 mg/ml)所引發[參見Newbould BB.,在大鼠中由分枝桿菌佐藥引發的關節炎的化療(Br J Pharmacol 1963;21:127-36)]。注射佐藥之前以及注射之後第14天,藉由LE 7500N型的體積差分儀(西班牙Panlab)測量被注射的爪的體積。
乳香脂酸部分、多醣部分與該組成物於口服200 mg/kg的劑量水平之下展現與劑量相關的水腫抑制作用(第七圖與表4)。與對照群相較,該組成物對於大鼠中結核菌引發的發炎性關節炎展現高度顯著的水腫抑制活性(抑制度為48%)。
表4:比較乳香脂酸部分、多醣類部分及本發明組成物對於對於大鼠(被注射的爪)中結核菌引發的發炎性關節炎的抗
關節炎活性(預防效果)
ASA:乙醯基水楊酸(標準)-100mg/kg;↓:抑制百分率
p數值*<0.01;**<0.001。
實施例4:於第14天進展中的發炎性關節炎爪組織的均質化
在為了每次分析測定(assay)的均質化之前,稱重含骨組織的冷凍爪,並在乾冰上將其碎裂成小塊。將爪組織加至萃取緩衝液(4 ml/g)組織中,該萃取緩衝液係含有1 mM PMSF(苯基甲基磺醯基氟化物(phenylmethylsulfonyl fluoride))、1 mg/ml抑肽酶(aprotinin)及0.05%Tween 20的磷酸鹽緩衝食鹽水。將組織在冰上用高速均質乳化機(Polytron)均質化,且將均質物以5000g離心15分鐘。將上清液保存在-80℃直至進行分析為止。參見[Anjali Pandey,Sarang Bani,Prabhu Dutt,Krishna Avtar Suri所發表的「在佐藥引發的關節炎組織中Th1/Th2細胞激素及發炎媒介物藉由羥基胡椒酚(hydroxychavicol)的調節」(Cytokine 49(2010) 114-121)]。
在來自組織均質物的上清液中的TNF-α、PGE2與LTB
4
的定量:
如上述於第14天製備來自不同群動物的樣品以供分析細胞激素媒介物。使用基於三明治及競爭性ELISA技術的市售套組(美國明尼蘇達州的R&D Systems公司),根據廠商提供的使用說明評估TNF-α、PGE2及LTB4
。所有的細胞激素濃度皆係藉由在ELISA盤讀取器(美國麻州的Multiskan,Thermo Electron公司提供)上於450 nm的比色測量值及從標準曲線的插值(interpolation)而求得。參見[Anjali Pandey,Sarang Bani,Prabhu Dutt,Krishna Avtar Suri所發表的「在佐藥引發的關節炎組織中Th1/Th2細胞激素及發炎媒介物藉由羥基胡椒酚的調節」(Cytokine 49(2010) 114-121)];[Magari K,Miyata S,Ohkubo Y,Mutoh S.所發表的「在大鼠膠原引發性關節炎的進展期間爪組織中的發炎細胞激素水平:FK506作為T細胞活性抑制劑的功效」(Inflamm Res 2004;53:469-74)]。在罹患關節炎的動物中,乳香脂酸部分顯示顯著降低TNF-α及LTB4
水平,但對於PGE2沒有顯著的抑制作用。多醣部分顯示可中度降低TNF-α及PGE2水平,但對於LTB4
沒有顯著的抑制作用;而該組成物顯著降低TNF-α、PGE2及LTB4
水平,其降低程度與劑量相關且在200 mg/kg的口服劑量下顯示最大的抑制度(第八圖、第九圖及第十圖)。
多醣部分對於PGE2顯示抑制作用的發現為本發明令人意外的發現,因此該發現具新穎性。由本研究可明顯看出該多醣部分單獨或與乳香脂酸組合可用於中等程度地抑制PGE2水平,不像是在本技藝中已知的其他藥物由於高度抑制PGE2而產生副作用。
實施例5:藉由流式細胞儀對於脾細胞中的細胞內IFN-γ的偵測:
關節炎的致病原因尚無法清楚地釐清,但累積的證據暗示T細胞媒介的自體免疫反應在其致病機制上扮演關鍵的角色[參見Panayi GS.所發表的「在類風濕性關節炎中的T細胞依賴性路徑」(Cur Opin Rheumatol 1977;9:236-40]。為了增加對關節炎治療的專一性,重心已轉向細胞激素。產生IFN的Th1細胞在人與動物二者的模型中對於關節炎的進展非常重要[Garra O.所發表的「細胞激素引發的功能性異質輔助性T細胞子集的發育」(Immunity 1998;8:275-83)]。因此,最近治療策略已經著重在調節Th1細胞的反應。藥效(pharmacodynamic)研究指出Th1/Th2調節在許多疾病狀況中可能為細胞激素療法的作用機制,參見[Lissoni P,Malugani F,Malysheva O.所發表的「用皮下低劑量白介素-2(interleukin-2)、褪黑激素(melatonin)及納曲酮(naltrexone)進行的頑固性轉移固態腫瘤的神經免疫療法以及藉由封阻類鴉片系統調節白介素-2引起的抗腫瘤免疫力」(Neuroendocrinol Lett 2002;23:341-4)];[Tabata N,Tagami H,Terui T.所發表的「在異位性皮膚炎中脫氫表雄固酮(dehydroepiandrosterone)可能為細胞激素產生的調解劑中之一個」(Arch Dermatol Res 1997;289:410-4)]。細胞媒介的免疫反應在關節炎進展期間扮演重要的角色[參見Waksman BH,Pearson CM,Sharp JT.所發表的「在大鼠中藉由注射分枝桿菌佐藥-II所引發的關節炎及其他損傷的研究:證明該疾病為對於外源性抗原的散布性免疫反應」(J Immunol 1960;85:403-17],以及此反應,尤其是CD4+T細胞所產生的IFN-γ的抑制,與具有抗關節炎活性的組成物有很強的相關性。該組成物對於CD4+T細胞所產生的IFN-γ產生劑量相關性抑制作用。
於無菌條件下,將來自所有試驗群動物的脾臟採集在Hank氏平衡鹽溶液(HBSS,Sigma)中,而取得均質的細胞懸浮液,並且使用FACS溶裂溶液溶裂紅血球。離心(380g,於4℃進行10分鐘)後,將粒化的細胞用PBS清洗3次,並重新懸浮於完全培養基中[補充有12 mM Hepes(pH 7.1)、0.05 mM 2-巰基乙醇、100 IU/ml盤尼西林(penicillin)、100 1g/ml鏈黴素(streptomycin)及10% FCS的RPMI 1640]。細胞數目係用血球計藉由錐蟲藍染料排斥技術來計算。細胞存活率超過95%。簡言之,將脾細胞懸浮液以2 x 106
個細胞/毫升之濃度接種於96-孔的平底微量滴定盤(Nunc)中。3日之後,將脾臟淋巴球用5 μl結合有PE的抗鼠IFN-γ抗體染色,並於存在1 μl的FACS滲透溶液下於4℃培養30分鐘。分析係使用Cell Quest Pro軟體在流式細胞儀(BD,LSR)上進行。[參見Anjali Pandey,Sarang Bani,Prabhu Dutt,Krishna Avtar Suri所發表的「在佐藥引發的關節炎組織中Th1/Th2細胞激素及發炎媒介物藉由羥基胡椒酚的調節」(Cytokine 49(2010) 114-121)]。我們利用螢光物質(flourochromes)培養脾細胞,以評估細胞內細胞激素含量。如預期,在關節炎對照群中IFN-γ的表現百分比較高(26.74%)。為了釐清IFN-γ相關性產生細胞激素淋巴球子集的特徵,檢視在CD4+T細胞之中產生IFN-γ的細胞。我們注意到來自以不同級位劑量的乳香脂酸部分、多醣部分及組成物治療的脾細胞的細胞內IFN gamma水平頗低。最大抑制度係在以200 mg/kg口服劑量的組成物治療的群中觀察到(第十一圖)。
實施例6:佐藥引發的確立發炎性關節炎:
關節炎係藉由將在油中的死亡結核菌注射在左爪之蹠下區而引發。疾病在前14天期逐漸形成,在此期間未投與任何藥物。從第15天開始至第28天,經口投與至該等動物。此試驗顯示該受試物質在已確立的關節炎中的治療潛力[Newbould,B.B.,1969所發表的「芬克洛酸(fenclozic acid),即2-(-4-氯苯基)噻唑-4-基乙酸:I.C.I. 54,450的療效;Myalex:一種具消炎、鎮痛及解熱活性的新穎化合物」(British Journal of Pharmacology. 35,189-197]。在用乳香脂酸部分、多醣部分及本發明組成物治療的動物中可觀察到絕對水腫抑制(absolute oedema inhibition)。但是,在已確立有關節炎的大鼠中該組成物顯示最為顯著的效果(絕對水腫的抑制)(第十二圖)。
因此,總體結果指出本發明之組成物比單獨的乳香脂酸部分及多醣部分顯示更為顯著的目標物抑制作用。在試管中最大作用出現在100及200 μg/ml時,而在實驗動物中,活體內最大作用出現在每日口服劑量為100及200 mg/kg動物體重時。
建議將該組成物以至多500 mg
,每日2至3次的劑量投與至需要其的個體。
第一圖:
在細胞外得自乳香屬的乳香脂酸部分於多種劑量下對於來自經治療Balb/c小鼠的血清中的活體內TNF-α及IL-1β估計值的影響。
第二圖:
在細胞外得自乳香屬的多醣部分於多種劑量下對於來自經治療Balb/c小鼠的血清中的活體內TNF-α及IL-1β估計值的影響。
第三圖:
在細胞外該組成物於多種劑量下對於來自經治療Balb/c小鼠的血清中的活體內TNF-α及IL-1β估計值的影響。
第四圖:
在細胞外得自乳香屬的乳香脂酸部分於多種劑量下對於來自經治療Balb/c小鼠的血清中的活體內NO估計值的影響。
第五圖:
在細胞外得自乳香屬的多醣部分於多種劑量下對於來自經治療Balb/c小鼠的血清中的活體內NO估計值的影響。
第 六圖:
在細胞外該組成物於多種劑量下對於來自經治療Balb/c小鼠的血清中的活體內NO估計值的影響。
第七圖:
比較乳香脂酸部分、多醣部分及其組成物對於大鼠中結核菌引發的發炎性關節炎(被注射的爪)的抗關節炎活性(預防效果)。
第八圖:
在以不同劑量水平的乳香脂酸部分、多醣部分及其組成物治療大鼠中結核菌引發的發炎性關節炎時,來自爪關節組織均質化物的上澄液中TNF-α的表現量。
第九圖:
在以不同劑量水平的乳香脂酸部分、多醣部分及其組成物治療大鼠中結核菌引發的發炎性關節炎時,來自爪關節組織均質化物的上澄液中PGE2的表現量。
第十圖:
在以不同劑量水平的乳香脂酸部分、多醣部分及其組成物治療大鼠中結核菌引發的發炎性關節炎時,來自爪關節組織均質化物的上澄液中LTB4的表現量。
第十一圖:
乳香脂酸部分、多醣部分及其組成物對於患有結核菌引發的發炎性關節炎的動物的脾細胞中,藉由流式細胞技術所測得的細胞內IFN-γ表現量的影響。
第十二圖:
乳香脂酸部分、多醣部分及其組成物(有效劑量)對於大鼠中已確立的結核菌引發性發炎性關節炎(被注射的爪)的抗關節炎活性(治療效果)。
第十三圖:
乳香脂酸部分與本發明組成物之水溶性比較。
第十四圖:
展現本發明組成物的製備步驟的流程圖。
Claims (2)
- 一種組成物之用途,其係用於製備下調/抑制一需要對象中PGE2之藥物,該組成物由多醣部分,視需要和醫藥上可接受的賦形劑所組成,其中該多醣類部分得自乳香屬植物。
- 如申請專利範圍第1項之用途,其中該需要對象為包括人類在內的動物。
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US30948110P | 2010-03-02 | 2010-03-02 | |
US12/768,871 US20110218172A1 (en) | 2010-03-02 | 2010-04-28 | Composition for down-regulating pro-inflammatory markers |
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TW201130498A TW201130498A (en) | 2011-09-16 |
TWI434696B true TWI434696B (zh) | 2014-04-21 |
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TW099129450A TWI434696B (zh) | 2010-03-02 | 2010-09-01 | 用於下調促發炎指標的組成物 |
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JP (1) | JP2011178773A (zh) |
KR (1) | KR20110099618A (zh) |
CN (1) | CN102218075A (zh) |
AU (1) | AU2011200854B2 (zh) |
BR (1) | BRPI1100700A2 (zh) |
EA (1) | EA023717B1 (zh) |
MX (1) | MX2011002161A (zh) |
TW (1) | TWI434696B (zh) |
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US8593634B1 (en) * | 2012-06-15 | 2013-11-26 | Larry Y Igarashi | Custom cosmetic blending machine |
AU2016393811B2 (en) * | 2016-02-24 | 2023-11-16 | Sami Labs Limited | Adaptogenic compositions and applications thereof |
US10716823B2 (en) * | 2016-02-24 | 2020-07-21 | Sami Labs Limited | Adaptogenic compositions and applications thereof |
CA3121148A1 (en) * | 2018-12-20 | 2020-06-25 | Hill's Pet Nutrition, Inc. | Pet food compositions |
WO2021217275A1 (en) * | 2020-04-30 | 2021-11-04 | Kondor Pharma Inc. | Immunomodulatory and antiviral action of boswellia gum resin extracts, derived formulations, and boswellic acids against respiratory viruses and uses thereof |
WO2022128052A1 (en) | 2020-12-14 | 2022-06-23 | Symrise Ag | Medicament for fighting inflammatory conditions of human skin |
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WO2003080092A1 (en) * | 2002-03-21 | 2003-10-02 | Council Of Scientific And Industrial Research | Water soluble bioactive fraction isolated from gum resin exudate of boswellia serrata, process for isolation thereof, composition containing said fraction and use thereof |
US7582314B2 (en) * | 2003-12-03 | 2009-09-01 | Sami Labs Ltd. | Compositions and methods for the management of hyperproliferative dermatological conditions |
CN101132693B (zh) * | 2004-08-02 | 2012-11-07 | 萨米莱布斯有限公司 | 用于控制过度增生性皮肤病的组合物以及方法 |
WO2007028256A2 (en) * | 2005-09-09 | 2007-03-15 | Les Biotechnologies Oceanova Inc. | Polysaccharides compositions comprising fucans and galactans and their use to reduce extravasation and inflammation |
US20080275117A1 (en) * | 2006-09-21 | 2008-11-06 | Dan Li | Compositions and Methods Comprising Boswellia Species |
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2010
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- 2010-08-31 JP JP2010193348A patent/JP2011178773A/ja active Pending
- 2010-09-01 CN CN2010102759899A patent/CN102218075A/zh active Pending
- 2010-09-01 TW TW099129450A patent/TWI434696B/zh active
- 2010-09-01 EA EA201001254A patent/EA023717B1/ru unknown
- 2010-09-01 KR KR1020100085731A patent/KR20110099618A/ko active Search and Examination
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- 2011-02-28 AU AU2011200854A patent/AU2011200854B2/en active Active
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EA023717B1 (ru) | 2016-07-29 |
MX2011002161A (es) | 2011-09-16 |
CN102218075A (zh) | 2011-10-19 |
AU2011200854A1 (en) | 2011-09-22 |
JP2011178773A (ja) | 2011-09-15 |
AU2011200854B2 (en) | 2016-10-20 |
KR20110099618A (ko) | 2011-09-08 |
TW201130498A (en) | 2011-09-16 |
EA201001254A1 (ru) | 2012-02-28 |
US20110218172A1 (en) | 2011-09-08 |
BRPI1100700A2 (pt) | 2013-12-17 |
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