TWI286159B - Improved stability of hybridisation interactions in dipstick assays - Google Patents

Improved stability of hybridisation interactions in dipstick assays Download PDF

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Publication number
TWI286159B
TWI286159B TW90116567A TW90116567A TWI286159B TW I286159 B TWI286159 B TW I286159B TW 90116567 A TW90116567 A TW 90116567A TW 90116567 A TW90116567 A TW 90116567A TW I286159 B TWI286159 B TW I286159B
Authority
TW
Taiwan
Prior art keywords
nucleic acid
probe
target nucleic
capture
detection
Prior art date
Application number
TW90116567A
Other languages
English (en)
Chinese (zh)
Inventor
Magda Anastassova Dineva
Helen Hwai-An Lee
Original Assignee
Diagnostics For The Real World
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from GBGB0016836.9A external-priority patent/GB0016836D0/en
Priority claimed from GB0016812A external-priority patent/GB0016812D0/en
Application filed by Diagnostics For The Real World filed Critical Diagnostics For The Real World
Application granted granted Critical
Publication of TWI286159B publication Critical patent/TWI286159B/zh

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6832—Enhancement of hybridisation reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
TW90116567A 2000-07-07 2001-07-06 Improved stability of hybridisation interactions in dipstick assays TWI286159B (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0016836.9A GB0016836D0 (en) 2000-07-07 2000-07-07 Improved dipstick assays (1)
GB0016812A GB0016812D0 (en) 2000-07-07 2000-07-07 Enhancing nucleic acid hybridisation

Publications (1)

Publication Number Publication Date
TWI286159B true TWI286159B (en) 2007-09-01

Family

ID=26244625

Family Applications (1)

Application Number Title Priority Date Filing Date
TW90116567A TWI286159B (en) 2000-07-07 2001-07-06 Improved stability of hybridisation interactions in dipstick assays

Country Status (3)

Country Link
AU (1) AU2001269292A1 (fr)
TW (1) TWI286159B (fr)
WO (1) WO2002004122A2 (fr)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8669052B2 (en) 2008-06-10 2014-03-11 Rapid Pathogen Screening, Inc. Lateral flow nucleic acid detector
US20110086359A1 (en) 2008-06-10 2011-04-14 Rapid Pathogen Screening, Inc. Lateral flow assays
JP2012503170A (ja) * 2008-07-15 2012-02-02 ラピッド パトゲン スクリーニング，インク． 側方流動イムノアッセイにおけるインサイツでの細胞の溶解
BR112012009023A2 (pt) * 2009-10-22 2019-09-24 Biotools Biotechnologial & Medical Laboratories S A método pra detecção. diferenciação e identificação de eubactérias específica de táxons em uma amostra biológica por meio de técnicas de análise de sequenciamento e kit
CN102154498B (zh) * 2011-03-21 2013-12-04 厦门大学 一种核酸的检测方法
JP2015164416A (ja) 2014-02-05 2015-09-17 扶桑薬品工業株式会社 マスクオリゴヌクレオチドを用いた核酸の検出、定量方法及びそのデバイス

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB8509367D0 (en) * 1985-04-12 1985-05-15 Amersham Int Plc Nucleic acid hybridisation
US4868105A (en) * 1985-12-11 1989-09-19 Chiron Corporation Solution phase nucleic acid sandwich assay
US5310650A (en) * 1986-09-29 1994-05-10 Abbott Laboratoires Method and device for improved reaction kinetics in nucleic acid hybridizations
WO1990001564A1 (fr) * 1988-08-09 1990-02-22 Microprobe Corporation Procedes d'analyse a cibles multiples par hybridation d'acides nucleiques
US5210015A (en) * 1990-08-06 1993-05-11 Hoffman-La Roche Inc. Homogeneous assay system using the nuclease activity of a nucleic acid polymerase
WO1994023299A1 (fr) * 1993-03-31 1994-10-13 Quidel Corporation Dispositif pour dosages multiples
EP0705426A4 (fr) * 1993-06-09 1998-07-08 Quidel Corp Titrages en une etape specifiques d'un antigene
WO1995027081A1 (fr) * 1994-03-31 1995-10-12 E.I. Du Pont De Nemours And Company Methode de detection de fragments d'acide nucleique

Also Published As

Publication number Publication date
WO2002004122A2 (fr) 2002-01-17
AU2001269292A1 (en) 2002-01-21
WO2002004122B1 (fr) 2003-08-07
WO2002004122A3 (fr) 2002-12-27

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Legal Events

Date Code Title Description
MM4A Annulment or lapse of patent due to non-payment of fees