TW202120688A - Rna導引之核酸酶及其活性片段及變體以及使用方法 - Google Patents
Rna導引之核酸酶及其活性片段及變體以及使用方法 Download PDFInfo
- Publication number
- TW202120688A TW202120688A TW109127218A TW109127218A TW202120688A TW 202120688 A TW202120688 A TW 202120688A TW 109127218 A TW109127218 A TW 109127218A TW 109127218 A TW109127218 A TW 109127218A TW 202120688 A TW202120688 A TW 202120688A
- Authority
- TW
- Taiwan
- Prior art keywords
- sequence
- rgn
- rna
- polypeptide
- seq
- Prior art date
Links
- 238000000034 method Methods 0.000 title claims abstract description 225
- 101710163270 Nuclease Proteins 0.000 title claims abstract description 164
- 239000012634 fragment Substances 0.000 title description 54
- 108020005004 Guide RNA Proteins 0.000 claims abstract description 334
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 259
- 102000004196 processed proteins & peptides Human genes 0.000 claims abstract description 256
- 229920001184 polypeptide Polymers 0.000 claims abstract description 254
- 108091028043 Nucleic acid sequence Proteins 0.000 claims abstract description 167
- 108091079001 CRISPR RNA Proteins 0.000 claims abstract description 141
- 239000013598 vector Substances 0.000 claims abstract description 106
- 150000007523 nucleic acids Chemical class 0.000 claims abstract description 99
- 102000039446 nucleic acids Human genes 0.000 claims abstract description 92
- 108020004707 nucleic acids Proteins 0.000 claims abstract description 92
- 230000027455 binding Effects 0.000 claims abstract description 30
- 238000009739 binding Methods 0.000 claims abstract description 30
- 239000000203 mixture Substances 0.000 claims abstract description 21
- 108091033409 CRISPR Proteins 0.000 claims abstract description 19
- 238000010354 CRISPR gene editing Methods 0.000 claims abstract 14
- 239000002773 nucleotide Substances 0.000 claims description 219
- 125000003729 nucleotide group Chemical group 0.000 claims description 216
- 102000040430 polynucleotide Human genes 0.000 claims description 212
- 108091033319 polynucleotide Proteins 0.000 claims description 212
- 239000002157 polynucleotide Substances 0.000 claims description 212
- 108020004414 DNA Proteins 0.000 claims description 171
- 230000008685 targeting Effects 0.000 claims description 147
- 102000053602 DNA Human genes 0.000 claims description 102
- 125000006850 spacer group Chemical group 0.000 claims description 58
- 125000003275 alpha amino acid group Chemical group 0.000 claims description 54
- 108020004682 Single-Stranded DNA Proteins 0.000 claims description 53
- 230000035772 mutation Effects 0.000 claims description 46
- 238000012217 deletion Methods 0.000 claims description 38
- 230000037430 deletion Effects 0.000 claims description 38
- 230000037431 insertion Effects 0.000 claims description 33
- 238000003780 insertion Methods 0.000 claims description 32
- 238000012360 testing method Methods 0.000 claims description 23
- 230000004048 modification Effects 0.000 claims description 19
- 238000012986 modification Methods 0.000 claims description 19
- 150000001413 amino acids Chemical class 0.000 claims description 18
- 230000015572 biosynthetic process Effects 0.000 claims description 16
- 230000006870 function Effects 0.000 claims description 15
- 238000013467 fragmentation Methods 0.000 claims description 12
- 238000006062 fragmentation reaction Methods 0.000 claims description 12
- 238000002866 fluorescence resonance energy transfer Methods 0.000 claims description 10
- 125000002652 ribonucleotide group Chemical group 0.000 claims description 9
- 108091028664 Ribonucleotide Proteins 0.000 claims description 8
- 239000002336 ribonucleotide Substances 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 5
- 238000012258 culturing Methods 0.000 claims description 5
- 108091032973 (ribonucleotides)n+m Proteins 0.000 claims 4
- 102000004882 Lipase Human genes 0.000 claims 2
- 108090001060 Lipase Proteins 0.000 claims 2
- 239000004367 Lipase Substances 0.000 claims 2
- 235000019421 lipase Nutrition 0.000 claims 2
- 108010044467 Isoenzymes Proteins 0.000 claims 1
- 125000003277 amino group Chemical group 0.000 claims 1
- 230000014509 gene expression Effects 0.000 abstract description 60
- 238000012800 visualization Methods 0.000 abstract description 2
- 210000004027 cell Anatomy 0.000 description 244
- 108090000623 proteins and genes Proteins 0.000 description 163
- 241000196324 Embryophyta Species 0.000 description 78
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 72
- 102100024640 Low-density lipoprotein receptor Human genes 0.000 description 58
- 102000004169 proteins and genes Human genes 0.000 description 57
- 206010033128 Ovarian cancer Diseases 0.000 description 53
- 206010061535 Ovarian neoplasm Diseases 0.000 description 53
- 235000018102 proteins Nutrition 0.000 description 52
- 201000010099 disease Diseases 0.000 description 47
- 230000000694 effects Effects 0.000 description 47
- 102000052609 BRCA2 Human genes 0.000 description 44
- 108700020462 BRCA2 Proteins 0.000 description 44
- 101150008921 Brca2 gene Proteins 0.000 description 44
- 238000006243 chemical reaction Methods 0.000 description 39
- 102000004389 Ribonucleoproteins Human genes 0.000 description 35
- 108010081734 Ribonucleoproteins Proteins 0.000 description 35
- 239000000047 product Substances 0.000 description 34
- 238000013518 transcription Methods 0.000 description 34
- 230000035897 transcription Effects 0.000 description 34
- 101000903703 Homo sapiens B-cell lymphoma/leukemia 11A Proteins 0.000 description 30
- 206010028980 Neoplasm Diseases 0.000 description 30
- 201000011510 cancer Diseases 0.000 description 30
- 108020001507 fusion proteins Proteins 0.000 description 30
- 102000037865 fusion proteins Human genes 0.000 description 30
- 102100022976 B-cell lymphoma/leukemia 11A Human genes 0.000 description 29
- 101001051093 Homo sapiens Low-density lipoprotein receptor Proteins 0.000 description 29
- 101000634835 Homo sapiens M1-specific T cell receptor alpha chain Proteins 0.000 description 29
- 101000634836 Homo sapiens T cell receptor alpha chain MC.7.G5 Proteins 0.000 description 29
- 208000000563 Hyperlipoproteinemia Type II Diseases 0.000 description 29
- 102100029450 M1-specific T cell receptor alpha chain Human genes 0.000 description 29
- 206010045261 Type IIa hyperlipidaemia Diseases 0.000 description 29
- 201000001386 familial hypercholesterolemia Diseases 0.000 description 29
- 210000002706 plastid Anatomy 0.000 description 28
- 238000003752 polymerase chain reaction Methods 0.000 description 28
- 210000001519 tissue Anatomy 0.000 description 27
- 238000003776 cleavage reaction Methods 0.000 description 25
- 238000000338 in vitro Methods 0.000 description 25
- 239000000523 sample Substances 0.000 description 25
- 208000011580 syndromic disease Diseases 0.000 description 25
- 208000035475 disorder Diseases 0.000 description 24
- 239000003623 enhancer Substances 0.000 description 24
- 230000007017 scission Effects 0.000 description 24
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 23
- 241000282414 Homo sapiens Species 0.000 description 23
- 108091026890 Coding region Proteins 0.000 description 22
- 210000003527 eukaryotic cell Anatomy 0.000 description 22
- 210000003463 organelle Anatomy 0.000 description 22
- 102000004190 Enzymes Human genes 0.000 description 20
- 108090000790 Enzymes Proteins 0.000 description 20
- 101000982032 Homo sapiens Myosin-binding protein C, cardiac-type Proteins 0.000 description 20
- 102100026771 Myosin-binding protein C, cardiac-type Human genes 0.000 description 20
- 108010077850 Nuclear Localization Signals Proteins 0.000 description 20
- 238000001514 detection method Methods 0.000 description 19
- 101150034941 AURKB gene Proteins 0.000 description 17
- 125000000539 amino acid group Chemical group 0.000 description 17
- 230000009977 dual effect Effects 0.000 description 17
- 239000000499 gel Substances 0.000 description 17
- 238000009396 hybridization Methods 0.000 description 17
- 210000001161 mammalian embryo Anatomy 0.000 description 17
- 108010008532 Deoxyribonuclease I Proteins 0.000 description 16
- 102000007260 Deoxyribonuclease I Human genes 0.000 description 16
- 238000003556 assay Methods 0.000 description 16
- 238000004519 manufacturing process Methods 0.000 description 16
- 102100026846 Cytidine deaminase Human genes 0.000 description 15
- 108010031325 Cytidine deaminase Proteins 0.000 description 15
- 210000004962 mammalian cell Anatomy 0.000 description 15
- 230000006780 non-homologous end joining Effects 0.000 description 15
- 230000001105 regulatory effect Effects 0.000 description 15
- 210000004899 c-terminal region Anatomy 0.000 description 14
- 230000001364 causal effect Effects 0.000 description 14
- 230000008569 process Effects 0.000 description 14
- 230000009466 transformation Effects 0.000 description 14
- 108010079245 Cystic Fibrosis Transmembrane Conductance Regulator Proteins 0.000 description 13
- 201000011252 Phenylketonuria Diseases 0.000 description 13
- 238000005516 engineering process Methods 0.000 description 13
- 102000008371 intracellularly ATP-gated chloride channel activity proteins Human genes 0.000 description 13
- 238000006467 substitution reaction Methods 0.000 description 13
- 241000701161 unidentified adenovirus Species 0.000 description 13
- 230000003612 virological effect Effects 0.000 description 13
- 101710169336 5'-deoxyadenosine deaminase Proteins 0.000 description 12
- 102000055025 Adenosine deaminases Human genes 0.000 description 12
- 108010054147 Hemoglobins Proteins 0.000 description 12
- 102000001554 Hemoglobins Human genes 0.000 description 12
- 239000000872 buffer Substances 0.000 description 12
- 230000008859 change Effects 0.000 description 12
- 230000000295 complement effect Effects 0.000 description 12
- 210000003743 erythrocyte Anatomy 0.000 description 12
- 238000010362 genome editing Methods 0.000 description 12
- 238000011282 treatment Methods 0.000 description 12
- 201000003883 Cystic fibrosis Diseases 0.000 description 11
- 208000026350 Inborn Genetic disease Diseases 0.000 description 11
- 241000700605 Viruses Species 0.000 description 11
- 230000002759 chromosomal effect Effects 0.000 description 11
- 230000005782 double-strand break Effects 0.000 description 11
- 230000004927 fusion Effects 0.000 description 11
- 238000004806 packaging method and process Methods 0.000 description 11
- 239000013603 viral vector Substances 0.000 description 11
- 102100034157 DNA mismatch repair protein Msh2 Human genes 0.000 description 10
- 208000008051 Hereditary Nonpolyposis Colorectal Neoplasms Diseases 0.000 description 10
- 206010051922 Hereditary non-polyposis colorectal cancer syndrome Diseases 0.000 description 10
- 101001134036 Homo sapiens DNA mismatch repair protein Msh2 Proteins 0.000 description 10
- 201000005027 Lynch syndrome Diseases 0.000 description 10
- 229910015837 MSH2 Inorganic materials 0.000 description 10
- 101000910035 Streptococcus pyogenes serotype M1 CRISPR-associated endonuclease Cas9/Csn1 Proteins 0.000 description 10
- 108010074605 gamma-Globulins Proteins 0.000 description 10
- 230000002068 genetic effect Effects 0.000 description 10
- 238000001727 in vivo Methods 0.000 description 10
- 230000001965 increasing effect Effects 0.000 description 10
- -1 nucleopasmin Proteins 0.000 description 10
- 230000008439 repair process Effects 0.000 description 10
- 108700028369 Alleles Proteins 0.000 description 9
- 208000031229 Cardiomyopathies Diseases 0.000 description 9
- 108020004705 Codon Proteins 0.000 description 9
- 101001087416 Homo sapiens Tyrosine-protein phosphatase non-receptor type 11 Proteins 0.000 description 9
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 9
- 102100033019 Tyrosine-protein phosphatase non-receptor type 11 Human genes 0.000 description 9
- 210000000746 body region Anatomy 0.000 description 9
- 210000003958 hematopoietic stem cell Anatomy 0.000 description 9
- 230000003252 repetitive effect Effects 0.000 description 9
- 238000005406 washing Methods 0.000 description 9
- 239000013607 AAV vector Substances 0.000 description 8
- 206010006187 Breast cancer Diseases 0.000 description 8
- 208000026310 Breast neoplasm Diseases 0.000 description 8
- 102100021519 Hemoglobin subunit beta Human genes 0.000 description 8
- 102000004913 RYR1 Human genes 0.000 description 8
- 108060007240 RYR1 Proteins 0.000 description 8
- 238000004520 electroporation Methods 0.000 description 8
- 210000002257 embryonic structure Anatomy 0.000 description 8
- 238000001415 gene therapy Methods 0.000 description 8
- 230000002829 reductive effect Effects 0.000 description 8
- 241000894007 species Species 0.000 description 8
- 230000009261 transgenic effect Effects 0.000 description 8
- 108700020463 BRCA1 Proteins 0.000 description 7
- 102000036365 BRCA1 Human genes 0.000 description 7
- 101150072950 BRCA1 gene Proteins 0.000 description 7
- 241000711573 Coronaviridae Species 0.000 description 7
- 241000238631 Hexapoda Species 0.000 description 7
- 230000004913 activation Effects 0.000 description 7
- 230000003321 amplification Effects 0.000 description 7
- 238000004458 analytical method Methods 0.000 description 7
- 239000012472 biological sample Substances 0.000 description 7
- 210000004369 blood Anatomy 0.000 description 7
- 239000008280 blood Substances 0.000 description 7
- 208000016361 genetic disease Diseases 0.000 description 7
- 239000003550 marker Substances 0.000 description 7
- 238000005259 measurement Methods 0.000 description 7
- 230000001404 mediated effect Effects 0.000 description 7
- 238000003199 nucleic acid amplification method Methods 0.000 description 7
- 238000011017 operating method Methods 0.000 description 7
- 230000005783 single-strand break Effects 0.000 description 7
- 239000000126 substance Substances 0.000 description 7
- 238000013519 translation Methods 0.000 description 7
- 102100035028 Alpha-L-iduronidase Human genes 0.000 description 6
- 241000193830 Bacillus <bacterium> Species 0.000 description 6
- 102100021147 DNA mismatch repair protein Msh6 Human genes 0.000 description 6
- 241000702421 Dependoparvovirus Species 0.000 description 6
- 102100031690 Erythroid transcription factor Human genes 0.000 description 6
- 108010044495 Fetal Hemoglobin Proteins 0.000 description 6
- 101000968658 Homo sapiens DNA mismatch repair protein Msh6 Proteins 0.000 description 6
- 101001066268 Homo sapiens Erythroid transcription factor Proteins 0.000 description 6
- 241001465754 Metazoa Species 0.000 description 6
- 206010056886 Mucopolysaccharidosis I Diseases 0.000 description 6
- IQFYYKKMVGJFEH-XLPZGREQSA-N Thymidine Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 IQFYYKKMVGJFEH-XLPZGREQSA-N 0.000 description 6
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 description 6
- 230000008901 benefit Effects 0.000 description 6
- 210000000349 chromosome Anatomy 0.000 description 6
- 239000002131 composite material Substances 0.000 description 6
- OPTASPLRGRRNAP-UHFFFAOYSA-N cytosine Chemical compound NC=1C=CNC(=O)N=1 OPTASPLRGRRNAP-UHFFFAOYSA-N 0.000 description 6
- 230000009615 deamination Effects 0.000 description 6
- 238000006481 deamination reaction Methods 0.000 description 6
- 239000011159 matrix material Substances 0.000 description 6
- 230000000149 penetrating effect Effects 0.000 description 6
- 239000002243 precursor Substances 0.000 description 6
- 210000001236 prokaryotic cell Anatomy 0.000 description 6
- 238000000746 purification Methods 0.000 description 6
- 230000022532 regulation of transcription, DNA-dependent Effects 0.000 description 6
- 238000012163 sequencing technique Methods 0.000 description 6
- 208000007056 sickle cell anemia Diseases 0.000 description 6
- 239000000243 solution Substances 0.000 description 6
- 238000012546 transfer Methods 0.000 description 6
- UHDGCWIWMRVCDJ-UHFFFAOYSA-N 1-beta-D-Xylofuranosyl-NH-Cytosine Natural products O=C1N=C(N)C=CN1C1C(O)C(O)C(CO)O1 UHDGCWIWMRVCDJ-UHFFFAOYSA-N 0.000 description 5
- 108010087504 Beta-Globulins Proteins 0.000 description 5
- UHDGCWIWMRVCDJ-PSQAKQOGSA-N Cytidine Natural products O=C1N=C(N)C=CN1[C@@H]1[C@@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-PSQAKQOGSA-N 0.000 description 5
- 241000701022 Cytomegalovirus Species 0.000 description 5
- 230000004568 DNA-binding Effects 0.000 description 5
- 108090000626 DNA-directed RNA polymerases Proteins 0.000 description 5
- 102000004163 DNA-directed RNA polymerases Human genes 0.000 description 5
- 208000012661 Dyskinesia Diseases 0.000 description 5
- 102100031780 Endonuclease Human genes 0.000 description 5
- 102100031509 Fibrillin-1 Human genes 0.000 description 5
- 101150103820 Fxn gene Proteins 0.000 description 5
- 102000006395 Globulins Human genes 0.000 description 5
- 108010044091 Globulins Proteins 0.000 description 5
- 108010043121 Green Fluorescent Proteins Proteins 0.000 description 5
- 102000004144 Green Fluorescent Proteins Human genes 0.000 description 5
- 101000896557 Homo sapiens Eukaryotic translation initiation factor 3 subunit B Proteins 0.000 description 5
- 101000846893 Homo sapiens Fibrillin-1 Proteins 0.000 description 5
- 101000899111 Homo sapiens Hemoglobin subunit beta Proteins 0.000 description 5
- 101000988834 Homo sapiens Hypoxanthine-guanine phosphoribosyltransferase Proteins 0.000 description 5
- 101001030243 Homo sapiens Myosin-7 Proteins 0.000 description 5
- 101000777277 Homo sapiens Serine/threonine-protein kinase Chk2 Proteins 0.000 description 5
- 208000015178 Hurler syndrome Diseases 0.000 description 5
- 102100029098 Hypoxanthine-guanine phosphoribosyltransferase Human genes 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 5
- 241000699670 Mus sp. Species 0.000 description 5
- 102100038934 Myosin-7 Human genes 0.000 description 5
- 208000009869 Neu-Laxova syndrome Diseases 0.000 description 5
- 206010029748 Noonan syndrome Diseases 0.000 description 5
- 102100031075 Serine/threonine-protein kinase Chk2 Human genes 0.000 description 5
- DBMJMQXJHONAFJ-UHFFFAOYSA-M Sodium laurylsulphate Chemical compound [Na+].CCCCCCCCCCCCOS([O-])(=O)=O DBMJMQXJHONAFJ-UHFFFAOYSA-M 0.000 description 5
- 230000002159 abnormal effect Effects 0.000 description 5
- 239000013592 cell lysate Substances 0.000 description 5
- UHDGCWIWMRVCDJ-ZAKLUEHWSA-N cytidine Chemical compound O=C1N=C(N)C=CN1[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O1 UHDGCWIWMRVCDJ-ZAKLUEHWSA-N 0.000 description 5
- 230000006378 damage Effects 0.000 description 5
- 229940079593 drug Drugs 0.000 description 5
- 239000003814 drug Substances 0.000 description 5
- 230000004049 epigenetic modification Effects 0.000 description 5
- 239000013604 expression vector Substances 0.000 description 5
- 108091006047 fluorescent proteins Proteins 0.000 description 5
- 102000034287 fluorescent proteins Human genes 0.000 description 5
- 238000010363 gene targeting Methods 0.000 description 5
- 230000010354 integration Effects 0.000 description 5
- 230000000670 limiting effect Effects 0.000 description 5
- 231100000350 mutagenesis Toxicity 0.000 description 5
- 239000002245 particle Substances 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 210000001938 protoplast Anatomy 0.000 description 5
- 238000011002 quantification Methods 0.000 description 5
- 230000006798 recombination Effects 0.000 description 5
- 238000005215 recombination Methods 0.000 description 5
- 238000001890 transfection Methods 0.000 description 5
- 230000001052 transient effect Effects 0.000 description 5
- 241001430294 unidentified retrovirus Species 0.000 description 5
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 description 4
- 108091093088 Amplicon Proteins 0.000 description 4
- 102000006734 Beta-Globulins Human genes 0.000 description 4
- 241000283690 Bos taurus Species 0.000 description 4
- 241000282472 Canis lupus familiaris Species 0.000 description 4
- 206010011878 Deafness Diseases 0.000 description 4
- 108010042407 Endonucleases Proteins 0.000 description 4
- 241000282326 Felis catus Species 0.000 description 4
- 101001019502 Homo sapiens Alpha-L-iduronidase Proteins 0.000 description 4
- 101000808011 Homo sapiens Vascular endothelial growth factor A Proteins 0.000 description 4
- 101150083522 MECP2 gene Proteins 0.000 description 4
- 241000124008 Mammalia Species 0.000 description 4
- 102100039124 Methyl-CpG-binding protein 2 Human genes 0.000 description 4
- 208000037538 Myelomonocytic Juvenile Leukemia Diseases 0.000 description 4
- 240000007594 Oryza sativa Species 0.000 description 4
- 235000007164 Oryza sativa Nutrition 0.000 description 4
- 102000014450 RNA Polymerase III Human genes 0.000 description 4
- 108010078067 RNA Polymerase III Proteins 0.000 description 4
- 102000015098 Tumor Suppressor Protein p53 Human genes 0.000 description 4
- 108010078814 Tumor Suppressor Protein p53 Proteins 0.000 description 4
- 102100039037 Vascular endothelial growth factor A Human genes 0.000 description 4
- 240000008042 Zea mays Species 0.000 description 4
- 235000002017 Zea mays subsp mays Nutrition 0.000 description 4
- 230000009418 agronomic effect Effects 0.000 description 4
- 238000013459 approach Methods 0.000 description 4
- 230000001580 bacterial effect Effects 0.000 description 4
- 230000003115 biocidal effect Effects 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000013461 design Methods 0.000 description 4
- 241001493065 dsRNA viruses Species 0.000 description 4
- 238000002474 experimental method Methods 0.000 description 4
- 239000005090 green fluorescent protein Substances 0.000 description 4
- 230000003394 haemopoietic effect Effects 0.000 description 4
- 230000002779 inactivation Effects 0.000 description 4
- 201000005992 juvenile myelomonocytic leukemia Diseases 0.000 description 4
- 208000032839 leukemia Diseases 0.000 description 4
- 238000001638 lipofection Methods 0.000 description 4
- 239000002502 liposome Substances 0.000 description 4
- 238000002844 melting Methods 0.000 description 4
- 230000008018 melting Effects 0.000 description 4
- 108020004999 messenger RNA Proteins 0.000 description 4
- 238000002703 mutagenesis Methods 0.000 description 4
- 210000004940 nucleus Anatomy 0.000 description 4
- 229920002401 polyacrylamide Polymers 0.000 description 4
- 230000001177 retroviral effect Effects 0.000 description 4
- 235000009566 rice Nutrition 0.000 description 4
- 150000003384 small molecules Chemical class 0.000 description 4
- 239000011780 sodium chloride Substances 0.000 description 4
- 230000009870 specific binding Effects 0.000 description 4
- 238000003786 synthesis reaction Methods 0.000 description 4
- 108091005957 yellow fluorescent proteins Proteins 0.000 description 4
- 102100035886 Adenine DNA glycosylase Human genes 0.000 description 3
- 241000589158 Agrobacterium Species 0.000 description 3
- 208000009575 Angelman syndrome Diseases 0.000 description 3
- 101700002522 BARD1 Proteins 0.000 description 3
- 102100028048 BRCA1-associated RING domain protein 1 Human genes 0.000 description 3
- 241000194110 Bacillus sp. (in: Bacteria) Species 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 206010008025 Cerebellar ataxia Diseases 0.000 description 3
- 241000611330 Chryseobacterium Species 0.000 description 3
- 206010053138 Congenital aplastic anaemia Diseases 0.000 description 3
- 208000012609 Cowden disease Diseases 0.000 description 3
- 201000002847 Cowden syndrome Diseases 0.000 description 3
- 102000016911 Deoxyribonucleases Human genes 0.000 description 3
- 108010053770 Deoxyribonucleases Proteins 0.000 description 3
- IAZDPXIOMUYVGZ-UHFFFAOYSA-N Dimethylsulphoxide Chemical compound CS(C)=O IAZDPXIOMUYVGZ-UHFFFAOYSA-N 0.000 description 3
- 201000004939 Fanconi anemia Diseases 0.000 description 3
- 102100036291 Galactose-1-phosphate uridylyltransferase Human genes 0.000 description 3
- 102100037156 Gap junction beta-2 protein Human genes 0.000 description 3
- 102000007513 Hemoglobin A Human genes 0.000 description 3
- 108010085682 Hemoglobin A Proteins 0.000 description 3
- 108091005904 Hemoglobin subunit beta Proteins 0.000 description 3
- 101001000351 Homo sapiens Adenine DNA glycosylase Proteins 0.000 description 3
- 101000954092 Homo sapiens Gap junction beta-2 protein Proteins 0.000 description 3
- 101000595746 Homo sapiens Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform Proteins 0.000 description 3
- 101000801643 Homo sapiens Retinal-specific phospholipid-transporting ATPase ABCA4 Proteins 0.000 description 3
- 101000951145 Homo sapiens Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial Proteins 0.000 description 3
- 208000023105 Huntington disease Diseases 0.000 description 3
- 101150022680 IDUA gene Proteins 0.000 description 3
- 102100021244 Integral membrane protein GPR180 Human genes 0.000 description 3
- 241000713666 Lentivirus Species 0.000 description 3
- 235000007688 Lycopersicon esculentum Nutrition 0.000 description 3
- 101710175625 Maltose/maltodextrin-binding periplasmic protein Proteins 0.000 description 3
- 108060004795 Methyltransferase Proteins 0.000 description 3
- 208000008770 Multiple Hamartoma Syndrome Diseases 0.000 description 3
- 208000021642 Muscular disease Diseases 0.000 description 3
- 201000009623 Myopathy Diseases 0.000 description 3
- 208000009905 Neurofibromatoses Diseases 0.000 description 3
- 241000283973 Oryctolagus cuniculus Species 0.000 description 3
- 102100036056 Phosphatidylinositol 4,5-bisphosphate 3-kinase catalytic subunit delta isoform Human genes 0.000 description 3
- 108010076504 Protein Sorting Signals Proteins 0.000 description 3
- 241000700159 Rattus Species 0.000 description 3
- 102100033617 Retinal-specific phospholipid-transporting ATPase ABCA4 Human genes 0.000 description 3
- 240000003768 Solanum lycopersicum Species 0.000 description 3
- 244000062793 Sorghum vulgare Species 0.000 description 3
- 241000191940 Staphylococcus Species 0.000 description 3
- 241000193996 Streptococcus pyogenes Species 0.000 description 3
- 108091027544 Subgenomic mRNA Proteins 0.000 description 3
- 102100038014 Succinate dehydrogenase [ubiquinone] cytochrome b small subunit, mitochondrial Human genes 0.000 description 3
- 208000002903 Thalassemia Diseases 0.000 description 3
- 102000040945 Transcription factor Human genes 0.000 description 3
- 108091023040 Transcription factor Proteins 0.000 description 3
- 238000007792 addition Methods 0.000 description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 230000008238 biochemical pathway Effects 0.000 description 3
- 210000003995 blood forming stem cell Anatomy 0.000 description 3
- 230000001413 cellular effect Effects 0.000 description 3
- 238000004891 communication Methods 0.000 description 3
- 230000003247 decreasing effect Effects 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 230000034431 double-strand break repair via homologous recombination Effects 0.000 description 3
- 239000000975 dye Substances 0.000 description 3
- 210000002950 fibroblast Anatomy 0.000 description 3
- 235000019688 fish Nutrition 0.000 description 3
- 238000001502 gel electrophoresis Methods 0.000 description 3
- 238000012239 gene modification Methods 0.000 description 3
- 230000012010 growth Effects 0.000 description 3
- 238000004128 high performance liquid chromatography Methods 0.000 description 3
- 238000002744 homologous recombination Methods 0.000 description 3
- 230000006801 homologous recombination Effects 0.000 description 3
- 230000002209 hydrophobic effect Effects 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 230000003993 interaction Effects 0.000 description 3
- 150000002632 lipids Chemical class 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- 230000007246 mechanism Effects 0.000 description 3
- 239000002609 medium Substances 0.000 description 3
- 238000010369 molecular cloning Methods 0.000 description 3
- VMGAPWLDMVPYIA-HIDZBRGKSA-N n'-amino-n-iminomethanimidamide Chemical compound N\N=C\N=N VMGAPWLDMVPYIA-HIDZBRGKSA-N 0.000 description 3
- 201000004931 neurofibromatosis Diseases 0.000 description 3
- 238000007481 next generation sequencing Methods 0.000 description 3
- 230000020520 nucleotide-excision repair Effects 0.000 description 3
- 210000000056 organ Anatomy 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 208000007312 paraganglioma Diseases 0.000 description 3
- BOLDJAUMGUJJKM-LSDHHAIUSA-N renifolin D Natural products CC(=C)[C@@H]1Cc2c(O)c(O)ccc2[C@H]1CC(=O)c3ccc(O)cc3O BOLDJAUMGUJJKM-LSDHHAIUSA-N 0.000 description 3
- 230000008521 reorganization Effects 0.000 description 3
- 230000010076 replication Effects 0.000 description 3
- 238000011160 research Methods 0.000 description 3
- 238000010839 reverse transcription Methods 0.000 description 3
- 238000012552 review Methods 0.000 description 3
- 238000012216 screening Methods 0.000 description 3
- 238000002864 sequence alignment Methods 0.000 description 3
- 238000002560 therapeutic procedure Methods 0.000 description 3
- 230000033587 transcription-coupled nucleotide-excision repair Effects 0.000 description 3
- 229940035893 uracil Drugs 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 241000203069 Archaea Species 0.000 description 2
- 241000972773 Aulopiformes Species 0.000 description 2
- 241000219310 Beta vulgaris subsp. vulgaris Species 0.000 description 2
- DWRXFEITVBNRMK-UHFFFAOYSA-N Beta-D-1-Arabinofuranosylthymine Natural products O=C1NC(=O)C(C)=CN1C1C(O)C(O)C(CO)O1 DWRXFEITVBNRMK-UHFFFAOYSA-N 0.000 description 2
- 101710201279 Biotin carboxyl carrier protein Proteins 0.000 description 2
- 241001678559 COVID-19 virus Species 0.000 description 2
- 101100468275 Caenorhabditis elegans rep-1 gene Proteins 0.000 description 2
- 235000002566 Capsicum Nutrition 0.000 description 2
- 201000002927 Cardiofaciocutaneous syndrome Diseases 0.000 description 2
- 201000002929 Carnitine palmitoyltransferase II deficiency Diseases 0.000 description 2
- 208000010693 Charcot-Marie-Tooth Disease Diseases 0.000 description 2
- 206010008723 Chondrodystrophy Diseases 0.000 description 2
- 241000056141 Chryseobacterium sp. Species 0.000 description 2
- 108700010070 Codon Usage Proteins 0.000 description 2
- 108091035707 Consensus sequence Proteins 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 2
- 244000241257 Cucumis melo Species 0.000 description 2
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 description 2
- 108010025464 Cyclin-Dependent Kinase 4 Proteins 0.000 description 2
- 102100036252 Cyclin-dependent kinase 4 Human genes 0.000 description 2
- 238000007400 DNA extraction Methods 0.000 description 2
- 102100034483 DNA repair protein RAD51 homolog 4 Human genes 0.000 description 2
- 208000002251 Dissecting Aneurysm Diseases 0.000 description 2
- 239000006144 Dulbecco’s modified Eagle's medium Substances 0.000 description 2
- 241000283086 Equidae Species 0.000 description 2
- 241000283074 Equus asinus Species 0.000 description 2
- 241000283073 Equus caballus Species 0.000 description 2
- 241000588724 Escherichia coli Species 0.000 description 2
- 241001198387 Escherichia coli BL21(DE3) Species 0.000 description 2
- 108060002716 Exonuclease Proteins 0.000 description 2
- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical compound NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 description 2
- 241000233866 Fungi Species 0.000 description 2
- 208000013135 GNE myopathy Diseases 0.000 description 2
- 241000287828 Gallus gallus Species 0.000 description 2
- 108700028146 Genetic Enhancer Elements Proteins 0.000 description 2
- 102100031181 Glyceraldehyde-3-phosphate dehydrogenase Human genes 0.000 description 2
- 235000010469 Glycine max Nutrition 0.000 description 2
- 244000068988 Glycine max Species 0.000 description 2
- 102100029880 Glycodelin Human genes 0.000 description 2
- 229940113491 Glycosylase inhibitor Drugs 0.000 description 2
- 244000299507 Gossypium hirsutum Species 0.000 description 2
- NYHBQMYGNKIUIF-UUOKFMHZSA-N Guanosine Chemical compound C1=NC=2C(=O)NC(N)=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O NYHBQMYGNKIUIF-UUOKFMHZSA-N 0.000 description 2
- 244000020551 Helianthus annuus Species 0.000 description 2
- 235000003222 Helianthus annuus Nutrition 0.000 description 2
- 102100031573 Hematopoietic progenitor cell antigen CD34 Human genes 0.000 description 2
- 108091027305 Heteroduplex Proteins 0.000 description 2
- 108010033040 Histones Proteins 0.000 description 2
- 102100023823 Homeobox protein EMX1 Human genes 0.000 description 2
- 101100493741 Homo sapiens BCL11A gene Proteins 0.000 description 2
- 101001132266 Homo sapiens DNA repair protein RAD51 homolog 4 Proteins 0.000 description 2
- 101000777663 Homo sapiens Hematopoietic progenitor cell antigen CD34 Proteins 0.000 description 2
- 101001048956 Homo sapiens Homeobox protein EMX1 Proteins 0.000 description 2
- 101000703761 Homo sapiens Leucine-rich repeat protein SHOC-2 Proteins 0.000 description 2
- 101001047090 Homo sapiens Potassium voltage-gated channel subfamily H member 2 Proteins 0.000 description 2
- 101001003584 Homo sapiens Prelamin-A/C Proteins 0.000 description 2
- 101001062129 Homo sapiens RNA-binding protein 20 Proteins 0.000 description 2
- 101000582767 Homo sapiens Regucalcin Proteins 0.000 description 2
- 101000874160 Homo sapiens Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Proteins 0.000 description 2
- 101000851334 Homo sapiens Troponin I, cardiac muscle Proteins 0.000 description 2
- 240000005979 Hordeum vulgare Species 0.000 description 2
- 235000007340 Hordeum vulgare Nutrition 0.000 description 2
- 241000725303 Human immunodeficiency virus Species 0.000 description 2
- VSNHCAURESNICA-UHFFFAOYSA-N Hydroxyurea Chemical compound NC(=O)NO VSNHCAURESNICA-UHFFFAOYSA-N 0.000 description 2
- 206010020649 Hyperkeratosis Diseases 0.000 description 2
- 108010003381 Iduronidase Proteins 0.000 description 2
- 206010061598 Immunodeficiency Diseases 0.000 description 2
- 208000029462 Immunodeficiency disease Diseases 0.000 description 2
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 108010025815 Kanamycin Kinase Proteins 0.000 description 2
- 102100031956 Leucine-rich repeat protein SHOC-2 Human genes 0.000 description 2
- 201000005978 Loeys-Dietz syndrome Diseases 0.000 description 2
- 206010025323 Lymphomas Diseases 0.000 description 2
- 201000011442 Metachromatic leukodystrophy Diseases 0.000 description 2
- 108010074346 Mismatch Repair Endonuclease PMS2 Proteins 0.000 description 2
- 102000008071 Mismatch Repair Endonuclease PMS2 Human genes 0.000 description 2
- 241000714177 Murine leukemia virus Species 0.000 description 2
- 241000282339 Mustela Species 0.000 description 2
- 241000208125 Nicotiana Species 0.000 description 2
- 235000002637 Nicotiana tabacum Nutrition 0.000 description 2
- 108091092724 Noncoding DNA Proteins 0.000 description 2
- 108020004485 Nonsense Codon Proteins 0.000 description 2
- 101150048484 Nrgn gene Proteins 0.000 description 2
- 101710153660 Nuclear receptor corepressor 2 Proteins 0.000 description 2
- 108091034117 Oligonucleotide Proteins 0.000 description 2
- 238000012408 PCR amplification Methods 0.000 description 2
- 241001494479 Pecora Species 0.000 description 2
- 239000006002 Pepper Substances 0.000 description 2
- 108091093037 Peptide nucleic acid Proteins 0.000 description 2
- 235000016761 Piper aduncum Nutrition 0.000 description 2
- 240000003889 Piper guineense Species 0.000 description 2
- 235000017804 Piper guineense Nutrition 0.000 description 2
- 235000008184 Piper nigrum Nutrition 0.000 description 2
- 206010035226 Plasma cell myeloma Diseases 0.000 description 2
- 102100022807 Potassium voltage-gated channel subfamily H member 2 Human genes 0.000 description 2
- 208000006664 Precursor Cell Lymphoblastic Leukemia-Lymphoma Diseases 0.000 description 2
- 102100026531 Prelamin-A/C Human genes 0.000 description 2
- 208000004777 Primary Hyperoxaluria Diseases 0.000 description 2
- 102100039233 Pyrin Human genes 0.000 description 2
- 108010059278 Pyrin Proteins 0.000 description 2
- 102100029248 RNA-binding protein 20 Human genes 0.000 description 2
- 238000003559 RNA-seq method Methods 0.000 description 2
- 102100030262 Regucalcin Human genes 0.000 description 2
- 108091081062 Repeated sequence (DNA) Proteins 0.000 description 2
- 241000589180 Rhizobium Species 0.000 description 2
- 241000589187 Rhizobium sp. Species 0.000 description 2
- 102000006382 Ribonucleases Human genes 0.000 description 2
- 108010083644 Ribonucleases Proteins 0.000 description 2
- 241000315672 SARS coronavirus Species 0.000 description 2
- 240000000111 Saccharum officinarum Species 0.000 description 2
- 235000007201 Saccharum officinarum Nutrition 0.000 description 2
- 201000004224 Schnyder corneal dystrophy Diseases 0.000 description 2
- 241000270295 Serpentes Species 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- 241000713311 Simian immunodeficiency virus Species 0.000 description 2
- 241000700584 Simplexvirus Species 0.000 description 2
- CDBYLPFSWZWCQE-UHFFFAOYSA-L Sodium Carbonate Chemical compound [Na+].[Na+].[O-]C([O-])=O CDBYLPFSWZWCQE-UHFFFAOYSA-L 0.000 description 2
- UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 2
- 235000002595 Solanum tuberosum Nutrition 0.000 description 2
- 244000061456 Solanum tuberosum Species 0.000 description 2
- 235000011684 Sorghum saccharatum Nutrition 0.000 description 2
- 208000027073 Stargardt disease Diseases 0.000 description 2
- 102100035726 Succinate dehydrogenase [ubiquinone] iron-sulfur subunit, mitochondrial Human genes 0.000 description 2
- 235000021536 Sugar beet Nutrition 0.000 description 2
- 241000282887 Suidae Species 0.000 description 2
- 241000282898 Sus scrofa Species 0.000 description 2
- 108010006785 Taq Polymerase Proteins 0.000 description 2
- 102100036407 Thioredoxin Human genes 0.000 description 2
- 201000008982 Thoracic Aortic Aneurysm Diseases 0.000 description 2
- 235000021307 Triticum Nutrition 0.000 description 2
- 244000098338 Triticum aestivum Species 0.000 description 2
- 102100036859 Troponin I, cardiac muscle Human genes 0.000 description 2
- 108010082433 UDP-glucose-hexose-1-phosphate uridylyltransferase Proteins 0.000 description 2
- 108090000848 Ubiquitin Proteins 0.000 description 2
- 102000044159 Ubiquitin Human genes 0.000 description 2
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 description 2
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 208000002517 adenoid cystic carcinoma Diseases 0.000 description 2
- 150000001412 amines Chemical class 0.000 description 2
- 210000004102 animal cell Anatomy 0.000 description 2
- 208000005980 beta thalassemia Diseases 0.000 description 2
- IQFYYKKMVGJFEH-UHFFFAOYSA-N beta-L-thymidine Natural products O=C1NC(=O)C(C)=CN1C1OC(CO)C(O)C1 IQFYYKKMVGJFEH-UHFFFAOYSA-N 0.000 description 2
- 230000004071 biological effect Effects 0.000 description 2
- 229960002685 biotin Drugs 0.000 description 2
- 235000020958 biotin Nutrition 0.000 description 2
- 239000011616 biotin Substances 0.000 description 2
- 238000010322 bone marrow transplantation Methods 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 239000006143 cell culture medium Substances 0.000 description 2
- 239000012707 chemical precursor Substances 0.000 description 2
- 235000013330 chicken meat Nutrition 0.000 description 2
- 102000021178 chitin binding proteins Human genes 0.000 description 2
- 108091011157 chitin binding proteins Proteins 0.000 description 2
- 150000001875 compounds Chemical class 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 235000005822 corn Nutrition 0.000 description 2
- 238000012937 correction Methods 0.000 description 2
- 230000001186 cumulative effect Effects 0.000 description 2
- 108010082025 cyan fluorescent protein Proteins 0.000 description 2
- 229940104302 cytosine Drugs 0.000 description 2
- 231100000895 deafness Toxicity 0.000 description 2
- 239000007857 degradation product Substances 0.000 description 2
- 239000005547 deoxyribonucleotide Substances 0.000 description 2
- 125000002637 deoxyribonucleotide group Chemical group 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000006471 dimerization reaction Methods 0.000 description 2
- 238000002224 dissection Methods 0.000 description 2
- WBZKQQHYRPRKNJ-UHFFFAOYSA-L disulfite Chemical compound [O-]S(=O)S([O-])(=O)=O WBZKQQHYRPRKNJ-UHFFFAOYSA-L 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 239000012636 effector Substances 0.000 description 2
- 230000007613 environmental effect Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 238000006911 enzymatic reaction Methods 0.000 description 2
- 238000002641 enzyme replacement therapy Methods 0.000 description 2
- 102000013165 exonuclease Human genes 0.000 description 2
- 230000001605 fetal effect Effects 0.000 description 2
- 210000003754 fetus Anatomy 0.000 description 2
- 239000007850 fluorescent dye Substances 0.000 description 2
- 238000003197 gene knockdown Methods 0.000 description 2
- 230000005017 genetic modification Effects 0.000 description 2
- 235000013617 genetically modified food Nutrition 0.000 description 2
- 238000012268 genome sequencing Methods 0.000 description 2
- 108020004445 glyceraldehyde-3-phosphate dehydrogenase Proteins 0.000 description 2
- 239000001963 growth medium Substances 0.000 description 2
- 208000016354 hearing loss disease Diseases 0.000 description 2
- 238000010438 heat treatment Methods 0.000 description 2
- 230000002363 herbicidal effect Effects 0.000 description 2
- 208000025581 hereditary breast carcinoma Diseases 0.000 description 2
- 210000005260 human cell Anatomy 0.000 description 2
- 229960001330 hydroxycarbamide Drugs 0.000 description 2
- 108010002685 hygromycin-B kinase Proteins 0.000 description 2
- 238000003384 imaging method Methods 0.000 description 2
- 238000001597 immobilized metal affinity chromatography Methods 0.000 description 2
- 230000007813 immunodeficiency Effects 0.000 description 2
- 208000014135 immunodeficiency 14 Diseases 0.000 description 2
- 238000001114 immunoprecipitation Methods 0.000 description 2
- 230000001976 improved effect Effects 0.000 description 2
- 238000011534 incubation Methods 0.000 description 2
- 208000015181 infectious disease Diseases 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
- 230000002401 inhibitory effect Effects 0.000 description 2
- 230000005764 inhibitory process Effects 0.000 description 2
- 230000000977 initiatory effect Effects 0.000 description 2
- 230000003902 lesion Effects 0.000 description 2
- 230000004807 localization Effects 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 208000003747 lymphoid leukemia Diseases 0.000 description 2
- 239000006166 lysate Substances 0.000 description 2
- 210000003712 lysosome Anatomy 0.000 description 2
- 230000001868 lysosomic effect Effects 0.000 description 2
- 235000009973 maize Nutrition 0.000 description 2
- 238000007726 management method Methods 0.000 description 2
- 230000035800 maturation Effects 0.000 description 2
- 201000001441 melanoma Diseases 0.000 description 2
- 239000012528 membrane Substances 0.000 description 2
- 230000011987 methylation Effects 0.000 description 2
- 238000007069 methylation reaction Methods 0.000 description 2
- 239000002679 microRNA Substances 0.000 description 2
- 238000000520 microinjection Methods 0.000 description 2
- 230000002438 mitochondrial effect Effects 0.000 description 2
- 230000025608 mitochondrion localization Effects 0.000 description 2
- 238000010172 mouse model Methods 0.000 description 2
- 239000003471 mutagenic agent Substances 0.000 description 2
- 231100000707 mutagenic chemical Toxicity 0.000 description 2
- 201000000050 myeloid neoplasm Diseases 0.000 description 2
- 230000037434 nonsense mutation Effects 0.000 description 2
- 230000030648 nucleus localization Effects 0.000 description 2
- 230000036961 partial effect Effects 0.000 description 2
- 230000037361 pathway Effects 0.000 description 2
- 239000008188 pellet Substances 0.000 description 2
- 230000035515 penetration Effects 0.000 description 2
- 230000025540 plastid localization Effects 0.000 description 2
- 230000008488 polyadenylation Effects 0.000 description 2
- 108020001580 protein domains Proteins 0.000 description 2
- 230000004850 protein–protein interaction Effects 0.000 description 2
- 230000002441 reversible effect Effects 0.000 description 2
- 201000007416 salivary gland adenoid cystic carcinoma Diseases 0.000 description 2
- 235000019515 salmon Nutrition 0.000 description 2
- 150000003839 salts Chemical class 0.000 description 2
- 208000002491 severe combined immunodeficiency Diseases 0.000 description 2
- 238000002741 site-directed mutagenesis Methods 0.000 description 2
- 229910001415 sodium ion Inorganic materials 0.000 description 2
- 210000004158 stalk cell Anatomy 0.000 description 2
- 210000000130 stem cell Anatomy 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 238000010381 tandem affinity purification Methods 0.000 description 2
- 230000001225 therapeutic effect Effects 0.000 description 2
- 108060008226 thioredoxin Proteins 0.000 description 2
- 229940094937 thioredoxin Drugs 0.000 description 2
- 229940104230 thymidine Drugs 0.000 description 2
- 238000010361 transduction Methods 0.000 description 2
- 230000026683 transduction Effects 0.000 description 2
- 238000011426 transformation method Methods 0.000 description 2
- 229910021642 ultra pure water Inorganic materials 0.000 description 2
- 239000012498 ultrapure water Substances 0.000 description 2
- 210000005167 vascular cell Anatomy 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 2
- 238000001262 western blot Methods 0.000 description 2
- MTCFGRXMJLQNBG-REOHCLBHSA-N (2S)-2-Amino-3-hydroxypropansäure Chemical compound OC[C@H](N)C(O)=O MTCFGRXMJLQNBG-REOHCLBHSA-N 0.000 description 1
- FQVLRGLGWNWPSS-BXBUPLCLSA-N (4r,7s,10s,13s,16r)-16-acetamido-13-(1h-imidazol-5-ylmethyl)-10-methyl-6,9,12,15-tetraoxo-7-propan-2-yl-1,2-dithia-5,8,11,14-tetrazacycloheptadecane-4-carboxamide Chemical compound N1C(=O)[C@@H](NC(C)=O)CSSC[C@@H](C(N)=O)NC(=O)[C@H](C(C)C)NC(=O)[C@H](C)NC(=O)[C@@H]1CC1=CN=CN1 FQVLRGLGWNWPSS-BXBUPLCLSA-N 0.000 description 1
- VSNHCAURESNICA-NJFSPNSNSA-N 1-oxidanylurea Chemical compound N[14C](=O)NO VSNHCAURESNICA-NJFSPNSNSA-N 0.000 description 1
- CKTSBUTUHBMZGZ-SHYZEUOFSA-N 2'‐deoxycytidine Chemical compound O=C1N=C(N)C=CN1[C@@H]1O[C@H](CO)[C@@H](O)C1 CKTSBUTUHBMZGZ-SHYZEUOFSA-N 0.000 description 1
- NHBKXEKEPDILRR-UHFFFAOYSA-N 2,3-bis(butanoylsulfanyl)propyl butanoate Chemical compound CCCC(=O)OCC(SC(=O)CCC)CSC(=O)CCC NHBKXEKEPDILRR-UHFFFAOYSA-N 0.000 description 1
- OVSKIKFHRZPJSS-UHFFFAOYSA-N 2,4-D Chemical compound OC(=O)COC1=CC=C(Cl)C=C1Cl OVSKIKFHRZPJSS-UHFFFAOYSA-N 0.000 description 1
- 239000005631 2,4-Dichlorophenoxyacetic acid Substances 0.000 description 1
- 229940087195 2,4-dichlorophenoxyacetate Drugs 0.000 description 1
- JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 1
- BFSVOASYOCHEOV-UHFFFAOYSA-N 2-diethylaminoethanol Chemical compound CCN(CC)CCO BFSVOASYOCHEOV-UHFFFAOYSA-N 0.000 description 1
- KQPKMEYBZUPZGK-UHFFFAOYSA-N 4-[(4-azido-2-nitroanilino)methyl]-5-(hydroxymethyl)-2-methylpyridin-3-ol Chemical compound CC1=NC=C(CO)C(CNC=2C(=CC(=CC=2)N=[N+]=[N-])[N+]([O-])=O)=C1O KQPKMEYBZUPZGK-UHFFFAOYSA-N 0.000 description 1
- 102100022681 40S ribosomal protein S27 Human genes 0.000 description 1
- 102100024626 5'-AMP-activated protein kinase subunit gamma-2 Human genes 0.000 description 1
- 102100036512 7-dehydrocholesterol reductase Human genes 0.000 description 1
- HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
- 102000007469 Actins Human genes 0.000 description 1
- 108010085238 Actins Proteins 0.000 description 1
- 102100032157 Adenylate cyclase type 10 Human genes 0.000 description 1
- 101150021974 Adh1 gene Proteins 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 208000005748 Aggressive Fibromatosis Diseases 0.000 description 1
- 241000589155 Agrobacterium tumefaciens Species 0.000 description 1
- 239000012099 Alexa Fluor family Substances 0.000 description 1
- 102100022712 Alpha-1-antitrypsin Human genes 0.000 description 1
- 108010068307 Alpha-Globulins Proteins 0.000 description 1
- 102000002572 Alpha-Globulins Human genes 0.000 description 1
- 241000143060 Americamysis bahia Species 0.000 description 1
- 241000224489 Amoeba Species 0.000 description 1
- 244000144725 Amygdalus communis Species 0.000 description 1
- 102000009091 Amyloidogenic Proteins Human genes 0.000 description 1
- 108010048112 Amyloidogenic Proteins Proteins 0.000 description 1
- 244000226021 Anacardium occidentale Species 0.000 description 1
- 244000099147 Ananas comosus Species 0.000 description 1
- 235000007119 Ananas comosus Nutrition 0.000 description 1
- 235000017060 Arachis glabrata Nutrition 0.000 description 1
- 244000105624 Arachis hypogaea Species 0.000 description 1
- 235000010777 Arachis hypogaea Nutrition 0.000 description 1
- 235000018262 Arachis monticola Nutrition 0.000 description 1
- 239000004475 Arginine Substances 0.000 description 1
- 206010003594 Ataxia telangiectasia Diseases 0.000 description 1
- 235000002147 Australian walnut Nutrition 0.000 description 1
- 229930192334 Auxin Natural products 0.000 description 1
- 235000007319 Avena orientalis Nutrition 0.000 description 1
- 244000075850 Avena orientalis Species 0.000 description 1
- 241000271566 Aves Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 108091032955 Bacterial small RNA Proteins 0.000 description 1
- BTBUEUYNUDRHOZ-UHFFFAOYSA-N Borate Chemical compound [O-]B([O-])[O-] BTBUEUYNUDRHOZ-UHFFFAOYSA-N 0.000 description 1
- 108091003079 Bovine Serum Albumin Proteins 0.000 description 1
- 241000458359 Brevibacillus sp. Species 0.000 description 1
- 235000004936 Bromus mango Nutrition 0.000 description 1
- 241000589562 Brucella Species 0.000 description 1
- 241000508772 Brucella sp. Species 0.000 description 1
- 102000000584 Calmodulin Human genes 0.000 description 1
- 108010041952 Calmodulin Proteins 0.000 description 1
- 101100507655 Canis lupus familiaris HSPA1 gene Proteins 0.000 description 1
- 229920000049 Carbon (fiber) Polymers 0.000 description 1
- 206010007269 Carcinogenicity Diseases 0.000 description 1
- 241000722731 Carex Species 0.000 description 1
- 235000009467 Carica papaya Nutrition 0.000 description 1
- 240000006432 Carica papaya Species 0.000 description 1
- 102100024853 Carnitine O-palmitoyltransferase 2, mitochondrial Human genes 0.000 description 1
- 108700005858 Carnitine palmitoyl transferase 2 deficiency Proteins 0.000 description 1
- 235000003255 Carthamus tinctorius Nutrition 0.000 description 1
- 244000020518 Carthamus tinctorius Species 0.000 description 1
- 241000701459 Caulimovirus Species 0.000 description 1
- 241000282693 Cercopithecidae Species 0.000 description 1
- 108091006146 Channels Proteins 0.000 description 1
- 108010077544 Chromatin Proteins 0.000 description 1
- 235000007516 Chrysanthemum Nutrition 0.000 description 1
- 244000189548 Chrysanthemum x morifolium Species 0.000 description 1
- 241000207199 Citrus Species 0.000 description 1
- 235000013162 Cocos nucifera Nutrition 0.000 description 1
- 244000060011 Cocos nucifera Species 0.000 description 1
- 101100007328 Cocos nucifera COS-1 gene Proteins 0.000 description 1
- 240000007154 Coffea arabica Species 0.000 description 1
- 206010009944 Colon cancer Diseases 0.000 description 1
- 208000001333 Colorectal Neoplasms Diseases 0.000 description 1
- 108020004635 Complementary DNA Proteins 0.000 description 1
- 108020004394 Complementary RNA Proteins 0.000 description 1
- 241000218631 Coniferophyta Species 0.000 description 1
- 208000017454 Cowden syndrome 3 Diseases 0.000 description 1
- MIKUYHXYGGJMLM-GIMIYPNGSA-N Crotonoside Natural products C1=NC2=C(N)NC(=O)N=C2N1[C@H]1O[C@@H](CO)[C@H](O)[C@@H]1O MIKUYHXYGGJMLM-GIMIYPNGSA-N 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 240000008067 Cucumis sativus Species 0.000 description 1
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 description 1
- 102100023381 Cyanocobalamin reductase / alkylcobalamin dealkylase Human genes 0.000 description 1
- 101710164985 Cyanocobalamin reductase / alkylcobalamin dealkylase Proteins 0.000 description 1
- NYHBQMYGNKIUIF-UHFFFAOYSA-N D-guanosine Natural products C1=2NC(N)=NC(=O)C=2N=CN1C1OC(CO)C(O)C1O NYHBQMYGNKIUIF-UHFFFAOYSA-N 0.000 description 1
- 108010066133 D-octopine dehydrogenase Proteins 0.000 description 1
- 238000007399 DNA isolation Methods 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 230000006820 DNA synthesis Effects 0.000 description 1
- 241000450599 DNA viruses Species 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- CKTSBUTUHBMZGZ-UHFFFAOYSA-N Deoxycytidine Natural products O=C1N=C(N)C=CN1C1OC(CO)C(O)C1 CKTSBUTUHBMZGZ-UHFFFAOYSA-N 0.000 description 1
- 229920000045 Dermatan sulfate Polymers 0.000 description 1
- 206010059352 Desmoid tumour Diseases 0.000 description 1
- 229920002307 Dextran Polymers 0.000 description 1
- 235000009355 Dianthus caryophyllus Nutrition 0.000 description 1
- 240000006497 Dianthus caryophyllus Species 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- 102100039246 Elongator complex protein 1 Human genes 0.000 description 1
- 101710167754 Elongator complex protein 1 Proteins 0.000 description 1
- 241000194032 Enterococcus faecalis Species 0.000 description 1
- 241001495410 Enterococcus sp. Species 0.000 description 1
- 241000588722 Escherichia Species 0.000 description 1
- 241000488157 Escherichia sp. Species 0.000 description 1
- 240000002395 Euphorbia pulcherrima Species 0.000 description 1
- 206010016207 Familial Mediterranean fever Diseases 0.000 description 1
- 208000001730 Familial dysautonomia Diseases 0.000 description 1
- 108010067741 Fanconi Anemia Complementation Group N protein Proteins 0.000 description 1
- 208000024412 Friedreich ataxia Diseases 0.000 description 1
- 241000702463 Geminiviridae Species 0.000 description 1
- 208000031448 Genomic Instability Diseases 0.000 description 1
- 241000713813 Gibbon ape leukemia virus Species 0.000 description 1
- KOSRFJWDECSPRO-WDSKDSINSA-N Glu-Glu Chemical compound OC(=O)CC[C@H](N)C(=O)N[C@@H](CCC(O)=O)C(O)=O KOSRFJWDECSPRO-WDSKDSINSA-N 0.000 description 1
- 102100036264 Glucose-6-phosphatase catalytic subunit 1 Human genes 0.000 description 1
- 108010070675 Glutathione transferase Proteins 0.000 description 1
- 102000004269 Granulocyte Colony-Stimulating Factor Human genes 0.000 description 1
- 108010017080 Granulocyte Colony-Stimulating Factor Proteins 0.000 description 1
- 101150013707 HBB gene Proteins 0.000 description 1
- 239000007995 HEPES buffer Substances 0.000 description 1
- 102100029100 Hematopoietic prostaglandin D synthase Human genes 0.000 description 1
- 102100027685 Hemoglobin subunit alpha Human genes 0.000 description 1
- 108091005902 Hemoglobin subunit alpha Proteins 0.000 description 1
- 108091005886 Hemoglobin subunit gamma Proteins 0.000 description 1
- 102100038617 Hemoglobin subunit gamma-2 Human genes 0.000 description 1
- 108010068250 Herpes Simplex Virus Protein Vmw65 Proteins 0.000 description 1
- 235000005206 Hibiscus Nutrition 0.000 description 1
- 235000007185 Hibiscus lunariifolius Nutrition 0.000 description 1
- 244000284380 Hibiscus rosa sinensis Species 0.000 description 1
- 102000008157 Histone Demethylases Human genes 0.000 description 1
- 108010074870 Histone Demethylases Proteins 0.000 description 1
- 102100039869 Histone H2B type F-S Human genes 0.000 description 1
- 102000011787 Histone Methyltransferases Human genes 0.000 description 1
- 108010036115 Histone Methyltransferases Proteins 0.000 description 1
- 102000003893 Histone acetyltransferases Human genes 0.000 description 1
- 108090000246 Histone acetyltransferases Proteins 0.000 description 1
- 102000043851 Histone deacetylase domains Human genes 0.000 description 1
- 108700038236 Histone deacetylase domains Proteins 0.000 description 1
- 102000006947 Histones Human genes 0.000 description 1
- 241000282412 Homo Species 0.000 description 1
- 101000678466 Homo sapiens 40S ribosomal protein S27 Proteins 0.000 description 1
- 101000760987 Homo sapiens 5'-AMP-activated protein kinase subunit gamma-2 Proteins 0.000 description 1
- 101000928720 Homo sapiens 7-dehydrocholesterol reductase Proteins 0.000 description 1
- 101000775498 Homo sapiens Adenylate cyclase type 10 Proteins 0.000 description 1
- 101000823116 Homo sapiens Alpha-1-antitrypsin Proteins 0.000 description 1
- 101000930910 Homo sapiens Glucose-6-phosphatase catalytic subunit 1 Proteins 0.000 description 1
- 101001035372 Homo sapiens Histone H2B type F-S Proteins 0.000 description 1
- 101000843809 Homo sapiens Hydroxycarboxylic acid receptor 2 Proteins 0.000 description 1
- 101000760730 Homo sapiens Medium-chain specific acyl-CoA dehydrogenase, mitochondrial Proteins 0.000 description 1
- 101001057159 Homo sapiens Melanoma-associated antigen C3 Proteins 0.000 description 1
- 101000615488 Homo sapiens Methyl-CpG-binding domain protein 2 Proteins 0.000 description 1
- 101000629029 Homo sapiens Myosin regulatory light chain 2, ventricular/cardiac muscle isoform Proteins 0.000 description 1
- 101001111984 Homo sapiens N-acylneuraminate-9-phosphatase Proteins 0.000 description 1
- 101000651201 Homo sapiens N-sulphoglucosamine sulphohydrolase Proteins 0.000 description 1
- 101001133640 Homo sapiens Phosphofurin acidic cluster sorting protein 1 Proteins 0.000 description 1
- 101000600434 Homo sapiens Putative uncharacterized protein encoded by MIR7-3HG Proteins 0.000 description 1
- 101001062093 Homo sapiens RNA-binding protein 15 Proteins 0.000 description 1
- 101000631899 Homo sapiens Ribosome maturation protein SBDS Proteins 0.000 description 1
- 101000629622 Homo sapiens Serine-pyruvate aminotransferase Proteins 0.000 description 1
- 101000984753 Homo sapiens Serine/threonine-protein kinase B-raf Proteins 0.000 description 1
- 101001074035 Homo sapiens Zinc finger protein GLI2 Proteins 0.000 description 1
- 241001135569 Human adenovirus 5 Species 0.000 description 1
- 241000713772 Human immunodeficiency virus 1 Species 0.000 description 1
- 244000267823 Hydrangea macrophylla Species 0.000 description 1
- 235000014486 Hydrangea macrophylla Nutrition 0.000 description 1
- 206010020751 Hypersensitivity Diseases 0.000 description 1
- 206010021143 Hypoxia Diseases 0.000 description 1
- 108020005350 Initiator Codon Proteins 0.000 description 1
- 244000017020 Ipomoea batatas Species 0.000 description 1
- 235000002678 Ipomoea batatas Nutrition 0.000 description 1
- 206010022971 Iron Deficiencies Diseases 0.000 description 1
- 241000588748 Klebsiella Species 0.000 description 1
- 241000588754 Klebsiella sp. Species 0.000 description 1
- AYFVYJQAPQTCCC-GBXIJSLDSA-N L-threonine Chemical compound C[C@@H](O)[C@H](N)C(O)=O AYFVYJQAPQTCCC-GBXIJSLDSA-N 0.000 description 1
- 238000007397 LAMP assay Methods 0.000 description 1
- 241000186610 Lactobacillus sp. Species 0.000 description 1
- 235000003228 Lactuca sativa Nutrition 0.000 description 1
- 240000008415 Lactuca sativa Species 0.000 description 1
- 101710128836 Large T antigen Proteins 0.000 description 1
- 241000270322 Lepidosauria Species 0.000 description 1
- 241000209510 Liliopsida Species 0.000 description 1
- 239000012097 Lipofectamine 2000 Substances 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 208000015439 Lysosomal storage disease Diseases 0.000 description 1
- 239000007993 MOPS buffer Substances 0.000 description 1
- 235000014826 Mangifera indica Nutrition 0.000 description 1
- 240000007228 Mangifera indica Species 0.000 description 1
- 240000003183 Manihot esculenta Species 0.000 description 1
- 235000016735 Manihot esculenta subsp esculenta Nutrition 0.000 description 1
- 101710132031 Mantle protein Proteins 0.000 description 1
- 208000001826 Marfan syndrome Diseases 0.000 description 1
- 240000004658 Medicago sativa Species 0.000 description 1
- 235000017587 Medicago sativa ssp. sativa Nutrition 0.000 description 1
- 108700000232 Medium chain acyl CoA dehydrogenase deficiency Proteins 0.000 description 1
- 206010072654 Medium-chain acyl-coenzyme A dehydrogenase deficiency Diseases 0.000 description 1
- 102100024590 Medium-chain specific acyl-CoA dehydrogenase, mitochondrial Human genes 0.000 description 1
- 101100409013 Mesembryanthemum crystallinum PPD gene Proteins 0.000 description 1
- 102100021299 Methyl-CpG-binding domain protein 2 Human genes 0.000 description 1
- 102000016397 Methyltransferase Human genes 0.000 description 1
- 108700011259 MicroRNAs Proteins 0.000 description 1
- 208000028781 Mucopolysaccharidosis type 1 Diseases 0.000 description 1
- 101000981253 Mus musculus GPI-linked NAD(P)(+)-arginine ADP-ribosyltransferase 1 Proteins 0.000 description 1
- 240000005561 Musa balbisiana Species 0.000 description 1
- 235000018290 Musa x paradisiaca Nutrition 0.000 description 1
- 102100038895 Myc proto-oncogene protein Human genes 0.000 description 1
- 101710135898 Myc proto-oncogene protein Proteins 0.000 description 1
- 241000204031 Mycoplasma Species 0.000 description 1
- 241000202944 Mycoplasma sp. Species 0.000 description 1
- 102100026925 Myosin regulatory light chain 2, ventricular/cardiac muscle isoform Human genes 0.000 description 1
- 241000118696 Myroides sp. Species 0.000 description 1
- 102100023906 N-acylneuraminate-9-phosphatase Human genes 0.000 description 1
- 102100027661 N-sulphoglucosamine sulphohydrolase Human genes 0.000 description 1
- 108091061960 Naked DNA Proteins 0.000 description 1
- 241000234479 Narcissus Species 0.000 description 1
- 241000244206 Nematoda Species 0.000 description 1
- 108700019961 Neoplasm Genes Proteins 0.000 description 1
- 102000048850 Neoplasm Genes Human genes 0.000 description 1
- 241000207746 Nicotiana benthamiana Species 0.000 description 1
- 108020004711 Nucleic Acid Probes Proteins 0.000 description 1
- 240000007817 Olea europaea Species 0.000 description 1
- 108700026244 Open Reading Frames Proteins 0.000 description 1
- 101150108119 PDS gene Proteins 0.000 description 1
- 108010011536 PTEN Phosphohydrolase Proteins 0.000 description 1
- 102000014160 PTEN Phosphohydrolase Human genes 0.000 description 1
- 102000005327 Palmitoyl protein thioesterase Human genes 0.000 description 1
- 108020002591 Palmitoyl protein thioesterase Proteins 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- 206010061332 Paraganglion neoplasm Diseases 0.000 description 1
- 102100040884 Partner and localizer of BRCA2 Human genes 0.000 description 1
- 244000025272 Persea americana Species 0.000 description 1
- 235000008673 Persea americana Nutrition 0.000 description 1
- 240000007377 Petunia x hybrida Species 0.000 description 1
- 235000010627 Phaseolus vulgaris Nutrition 0.000 description 1
- 244000046052 Phaseolus vulgaris Species 0.000 description 1
- 102100034078 Phosphofurin acidic cluster sorting protein 1 Human genes 0.000 description 1
- 235000010582 Pisum sativum Nutrition 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 241000589516 Pseudomonas Species 0.000 description 1
- 241000589774 Pseudomonas sp. Species 0.000 description 1
- 241000508269 Psidium Species 0.000 description 1
- 102100037401 Putative uncharacterized protein encoded by MIR7-3HG Human genes 0.000 description 1
- 101150111251 RGN gene Proteins 0.000 description 1
- 230000004570 RNA-binding Effects 0.000 description 1
- 102100029244 RNA-binding protein 15 Human genes 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 241000245165 Rhododendron ponticum Species 0.000 description 1
- 102100028750 Ribosome maturation protein SBDS Human genes 0.000 description 1
- 201000001638 Riley-Day syndrome Diseases 0.000 description 1
- 241000220317 Rosa Species 0.000 description 1
- 241001452558 SARS bat coronavirus Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 101100170553 Saccharomyces cerevisiae (strain ATCC 204508 / S288c) DLD2 gene Proteins 0.000 description 1
- 241000607142 Salmonella Species 0.000 description 1
- 241000607149 Salmonella sp. Species 0.000 description 1
- 244000082988 Secale cereale Species 0.000 description 1
- 235000007238 Secale cereale Nutrition 0.000 description 1
- MTCFGRXMJLQNBG-UHFFFAOYSA-N Serine Natural products OCC(N)C(O)=O MTCFGRXMJLQNBG-UHFFFAOYSA-N 0.000 description 1
- 102100026842 Serine-pyruvate aminotransferase Human genes 0.000 description 1
- 102100027103 Serine/threonine-protein kinase B-raf Human genes 0.000 description 1
- 108010071390 Serum Albumin Proteins 0.000 description 1
- 102000007562 Serum Albumin Human genes 0.000 description 1
- 241000607768 Shigella Species 0.000 description 1
- 241000607758 Shigella sp. Species 0.000 description 1
- 201000004283 Shwachman-Diamond syndrome Diseases 0.000 description 1
- 108010016797 Sickle Hemoglobin Proteins 0.000 description 1
- 108091061750 Signal recognition particle RNA Proteins 0.000 description 1
- 108020004459 Small interfering RNA Proteins 0.000 description 1
- 201000007410 Smith-Lemli-Opitz syndrome Diseases 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 241000685602 Sphingobacterium sp. Species 0.000 description 1
- 241000736131 Sphingomonas Species 0.000 description 1
- 235000009184 Spondias indica Nutrition 0.000 description 1
- 241001147693 Staphylococcus sp. Species 0.000 description 1
- 244000057717 Streptococcus lactis Species 0.000 description 1
- 241000187747 Streptomyces Species 0.000 description 1
- 241000187180 Streptomyces sp. Species 0.000 description 1
- QAOWNCQODCNURD-UHFFFAOYSA-L Sulfate Chemical compound [O-]S([O-])(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-L 0.000 description 1
- LSNNMFCWUKXFEE-UHFFFAOYSA-N Sulfurous acid Chemical compound OS(O)=O LSNNMFCWUKXFEE-UHFFFAOYSA-N 0.000 description 1
- 238000010459 TALEN Methods 0.000 description 1
- 206010043395 Thalassaemia sickle cell Diseases 0.000 description 1
- 244000269722 Thea sinensis Species 0.000 description 1
- 244000299461 Theobroma cacao Species 0.000 description 1
- 235000009470 Theobroma cacao Nutrition 0.000 description 1
- AYFVYJQAPQTCCC-UHFFFAOYSA-N Threonine Natural products CC(O)C(N)C(O)=O AYFVYJQAPQTCCC-UHFFFAOYSA-N 0.000 description 1
- 239000004473 Threonine Substances 0.000 description 1
- 108010043645 Transcription Activator-Like Effector Nucleases Proteins 0.000 description 1
- 108700009124 Transcription Initiation Site Proteins 0.000 description 1
- 101710150448 Transcriptional regulator Myc Proteins 0.000 description 1
- 108020004566 Transfer RNA Proteins 0.000 description 1
- 108010039203 Tripeptidyl-Peptidase 1 Proteins 0.000 description 1
- 102100034197 Tripeptidyl-peptidase 1 Human genes 0.000 description 1
- 239000007983 Tris buffer Substances 0.000 description 1
- 241000722921 Tulipa gesneriana Species 0.000 description 1
- 102000001742 Tumor Suppressor Proteins Human genes 0.000 description 1
- 108010040002 Tumor Suppressor Proteins Proteins 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 241000607284 Vibrio sp. Species 0.000 description 1
- 240000004922 Vigna radiata Species 0.000 description 1
- 235000010721 Vigna radiata var radiata Nutrition 0.000 description 1
- 235000011469 Vigna radiata var sublobata Nutrition 0.000 description 1
- 108020005202 Viral DNA Proteins 0.000 description 1
- 108700005077 Viral Genes Proteins 0.000 description 1
- 208000036142 Viral infection Diseases 0.000 description 1
- 208000027276 Von Willebrand disease Diseases 0.000 description 1
- 241000607734 Yersinia <bacteria> Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 241000131891 Yersinia sp. Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 101710185494 Zinc finger protein Proteins 0.000 description 1
- 102100023597 Zinc finger protein 816 Human genes 0.000 description 1
- 102100035558 Zinc finger protein GLI2 Human genes 0.000 description 1
- 238000009825 accumulation Methods 0.000 description 1
- 230000021736 acetylation Effects 0.000 description 1
- 238000006640 acetylation reaction Methods 0.000 description 1
- 208000005065 achondrogenesis Diseases 0.000 description 1
- 208000008919 achondroplasia Diseases 0.000 description 1
- 210000005221 acidic domain Anatomy 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 239000000853 adhesive Substances 0.000 description 1
- 230000002411 adverse Effects 0.000 description 1
- 238000001042 affinity chromatography Methods 0.000 description 1
- 238000000246 agarose gel electrophoresis Methods 0.000 description 1
- 208000026935 allergic disease Diseases 0.000 description 1
- 235000020224 almond Nutrition 0.000 description 1
- 208000006682 alpha 1-Antitrypsin Deficiency Diseases 0.000 description 1
- 201000006288 alpha thalassemia Diseases 0.000 description 1
- KOSRFJWDECSPRO-UHFFFAOYSA-N alpha-L-glutamyl-L-glutamic acid Natural products OC(=O)CCC(N)C(=O)NC(CCC(O)=O)C(O)=O KOSRFJWDECSPRO-UHFFFAOYSA-N 0.000 description 1
- 230000006229 amino acid addition Effects 0.000 description 1
- 101150073130 ampR gene Proteins 0.000 description 1
- 230000003942 amyloidogenic effect Effects 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 108091007433 antigens Proteins 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 1
- 230000006217 arginine-methylation Effects 0.000 description 1
- 238000003491 array Methods 0.000 description 1
- 208000025341 autosomal recessive disease Diseases 0.000 description 1
- 239000002363 auxin Substances 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000012148 binding buffer Substances 0.000 description 1
- 230000008033 biological extinction Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 238000001574 biopsy Methods 0.000 description 1
- 201000000053 blastoma Diseases 0.000 description 1
- 239000007853 buffer solution Substances 0.000 description 1
- 239000007975 buffered saline Substances 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 239000001506 calcium phosphate Substances 0.000 description 1
- 229910000389 calcium phosphate Inorganic materials 0.000 description 1
- 235000011010 calcium phosphates Nutrition 0.000 description 1
- 239000004917 carbon fiber Substances 0.000 description 1
- 125000003178 carboxy group Chemical group [H]OC(*)=O 0.000 description 1
- 230000007670 carcinogenicity Effects 0.000 description 1
- 231100000260 carcinogenicity Toxicity 0.000 description 1
- 235000020226 cashew nut Nutrition 0.000 description 1
- 125000002091 cationic group Chemical group 0.000 description 1
- 150000001768 cations Chemical class 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000032823 cell division Effects 0.000 description 1
- 238000002659 cell therapy Methods 0.000 description 1
- 230000010307 cell transformation Effects 0.000 description 1
- 230000004700 cellular uptake Effects 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 210000001175 cerebrospinal fluid Anatomy 0.000 description 1
- 238000002655 chelation therapy Methods 0.000 description 1
- 125000003636 chemical group Chemical group 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 210000003763 chloroplast Anatomy 0.000 description 1
- 210000003483 chromatin Anatomy 0.000 description 1
- 238000002487 chromatin immunoprecipitation Methods 0.000 description 1
- 238000004587 chromatography analysis Methods 0.000 description 1
- 239000013611 chromosomal DNA Substances 0.000 description 1
- 230000008711 chromosomal rearrangement Effects 0.000 description 1
- 230000001684 chronic effect Effects 0.000 description 1
- 235000020971 citrus fruits Nutrition 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000000975 co-precipitation Methods 0.000 description 1
- 235000016213 coffee Nutrition 0.000 description 1
- 235000013353 coffee beverage Nutrition 0.000 description 1
- 239000003184 complementary RNA Substances 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 239000012141 concentrate Substances 0.000 description 1
- 238000012790 confirmation Methods 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 210000004748 cultured cell Anatomy 0.000 description 1
- 230000009089 cytolysis Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000012350 deep sequencing Methods 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000002716 delivery method Methods 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- AVJBPWGFOQAPRH-FWMKGIEWSA-L dermatan sulfate Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@H](OS([O-])(=O)=O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O)[C@H](C([O-])=O)O1 AVJBPWGFOQAPRH-FWMKGIEWSA-L 0.000 description 1
- 229940051593 dermatan sulfate Drugs 0.000 description 1
- 201000006827 desmoid tumor Diseases 0.000 description 1
- 230000000368 destabilizing effect Effects 0.000 description 1
- 230000001066 destructive effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000003745 diagnosis Methods 0.000 description 1
- 235000014113 dietary fatty acids Nutrition 0.000 description 1
- LOKCTEFSRHRXRJ-UHFFFAOYSA-I dipotassium trisodium dihydrogen phosphate hydrogen phosphate dichloride Chemical compound P(=O)(O)(O)[O-].[K+].P(=O)(O)([O-])[O-].[Na+].[Na+].[Cl-].[K+].[Cl-].[Na+] LOKCTEFSRHRXRJ-UHFFFAOYSA-I 0.000 description 1
- 208000016097 disease of metabolism Diseases 0.000 description 1
- 208000037765 diseases and disorders Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000007877 drug screening Methods 0.000 description 1
- 210000005069 ears Anatomy 0.000 description 1
- 201000008184 embryoma Diseases 0.000 description 1
- 238000000295 emission spectrum Methods 0.000 description 1
- 108010048367 enhanced green fluorescent protein Proteins 0.000 description 1
- 229940032049 enterococcus faecalis Drugs 0.000 description 1
- 230000007608 epigenetic mechanism Effects 0.000 description 1
- 210000000267 erythroid cell Anatomy 0.000 description 1
- 229940011871 estrogen Drugs 0.000 description 1
- 239000000262 estrogen Substances 0.000 description 1
- 241001233957 eudicotyledons Species 0.000 description 1
- 238000000695 excitation spectrum Methods 0.000 description 1
- 230000005281 excited state Effects 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
- 239000000194 fatty acid Substances 0.000 description 1
- 150000004665 fatty acids Chemical class 0.000 description 1
- 239000012091 fetal bovine serum Substances 0.000 description 1
- GNBHRKFJIUUOQI-UHFFFAOYSA-N fluorescein Chemical compound O1C(=O)C2=CC=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 GNBHRKFJIUUOQI-UHFFFAOYSA-N 0.000 description 1
- MHMNJMPURVTYEJ-UHFFFAOYSA-N fluorescein-5-isothiocyanate Chemical compound O1C(=O)C2=CC(N=C=S)=CC=C2C21C1=CC=C(O)C=C1OC1=CC(O)=CC=C21 MHMNJMPURVTYEJ-UHFFFAOYSA-N 0.000 description 1
- 239000003205 fragrance Substances 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 102000034356 gene-regulatory proteins Human genes 0.000 description 1
- 108091006104 gene-regulatory proteins Proteins 0.000 description 1
- 230000009395 genetic defect Effects 0.000 description 1
- 238000010353 genetic engineering Methods 0.000 description 1
- 230000007614 genetic variation Effects 0.000 description 1
- 235000003869 genetically modified organism Nutrition 0.000 description 1
- 210000004602 germ cell Anatomy 0.000 description 1
- 108010055341 glutamyl-glutamic acid Proteins 0.000 description 1
- 125000003055 glycidyl group Chemical group C(C1CO1)* 0.000 description 1
- 208000011460 glycogen storage disease due to glucose-6-phosphatase deficiency type IA Diseases 0.000 description 1
- 229940029575 guanosine Drugs 0.000 description 1
- 150000003278 haem Chemical group 0.000 description 1
- 238000003306 harvesting Methods 0.000 description 1
- 230000035876 healing Effects 0.000 description 1
- 208000034737 hemoglobinopathy Diseases 0.000 description 1
- 239000004009 herbicide Substances 0.000 description 1
- 208000012681 hereditary desmoid disease Diseases 0.000 description 1
- 102000052096 human BCL11A Human genes 0.000 description 1
- 210000005119 human aortic smooth muscle cell Anatomy 0.000 description 1
- 238000011577 humanized mouse model Methods 0.000 description 1
- 230000007062 hydrolysis Effects 0.000 description 1
- 238000006460 hydrolysis reaction Methods 0.000 description 1
- 238000007031 hydroxymethylation reaction Methods 0.000 description 1
- 230000009610 hypersensitivity Effects 0.000 description 1
- 230000007954 hypoxia Effects 0.000 description 1
- 230000014726 immortalization of host cell Effects 0.000 description 1
- 238000010348 incorporation Methods 0.000 description 1
- SEOVTRFCIGRIMH-UHFFFAOYSA-N indole-3-acetic acid Chemical compound C1=CC=C2C(CC(=O)O)=CNC2=C1 SEOVTRFCIGRIMH-UHFFFAOYSA-N 0.000 description 1
- 238000001802 infusion Methods 0.000 description 1
- 230000002452 interceptive effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 210000003292 kidney cell Anatomy 0.000 description 1
- 210000001985 kidney epithelial cell Anatomy 0.000 description 1
- 238000011005 laboratory method Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 208000004731 long QT syndrome Diseases 0.000 description 1
- 125000003588 lysine group Chemical group [H]N([H])C([H])([H])C([H])([H])C([H])([H])C([H])([H])C([H])(N([H])[H])C(*)=O 0.000 description 1
- 230000014759 maintenance of location Effects 0.000 description 1
- 208000005548 medium chain acyl-CoA dehydrogenase deficiency Diseases 0.000 description 1
- 208000030159 metabolic disease Diseases 0.000 description 1
- VNWKTOKETHGBQD-UHFFFAOYSA-N methane Chemical compound C VNWKTOKETHGBQD-UHFFFAOYSA-N 0.000 description 1
- MGJXBDMLVWIYOQ-UHFFFAOYSA-N methylazanide Chemical compound [NH-]C MGJXBDMLVWIYOQ-UHFFFAOYSA-N 0.000 description 1
- 108091070501 miRNA Proteins 0.000 description 1
- 235000019713 millet Nutrition 0.000 description 1
- 230000003278 mimic effect Effects 0.000 description 1
- 210000003470 mitochondria Anatomy 0.000 description 1
- 238000002715 modification method Methods 0.000 description 1
- 238000001823 molecular biology technique Methods 0.000 description 1
- 210000004897 n-terminal region Anatomy 0.000 description 1
- 239000013642 negative control Substances 0.000 description 1
- 210000000944 nerve tissue Anatomy 0.000 description 1
- 230000001537 neural effect Effects 0.000 description 1
- 201000007642 neuronal ceroid lipofuscinosis 1 Diseases 0.000 description 1
- 230000007935 neutral effect Effects 0.000 description 1
- 238000006386 neutralization reaction Methods 0.000 description 1
- 230000009871 nonspecific binding Effects 0.000 description 1
- 108010058731 nopaline synthase Proteins 0.000 description 1
- 238000007899 nucleic acid hybridization Methods 0.000 description 1
- 239000002853 nucleic acid probe Substances 0.000 description 1
- 230000000414 obstructive effect Effects 0.000 description 1
- 230000004792 oxidative damage Effects 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 239000013610 patient sample Substances 0.000 description 1
- 235000020232 peanut Nutrition 0.000 description 1
- 239000002953 phosphate buffered saline Substances 0.000 description 1
- 229930029653 phosphoenolpyruvate Natural products 0.000 description 1
- 230000026731 phosphorylation Effects 0.000 description 1
- 238000006366 phosphorylation reaction Methods 0.000 description 1
- 238000003976 plant breeding Methods 0.000 description 1
- 230000037039 plant physiology Effects 0.000 description 1
- 210000002381 plasma Anatomy 0.000 description 1
- 239000013612 plasmid Substances 0.000 description 1
- 229920000642 polymer Polymers 0.000 description 1
- 102000054765 polymorphisms of proteins Human genes 0.000 description 1
- 230000023603 positive regulation of transcription initiation, DNA-dependent Effects 0.000 description 1
- 101150063097 ppdK gene Proteins 0.000 description 1
- 239000002244 precipitate Substances 0.000 description 1
- 230000002028 premature Effects 0.000 description 1
- 125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 230000000750 progressive effect Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000000159 protein binding assay Methods 0.000 description 1
- 235000004252 protein component Nutrition 0.000 description 1
- 238000010379 pull-down assay Methods 0.000 description 1
- 239000002096 quantum dot Substances 0.000 description 1
- 230000002285 radioactive effect Effects 0.000 description 1
- 239000000376 reactant Substances 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 230000007115 recruitment Effects 0.000 description 1
- 239000013074 reference sample Substances 0.000 description 1
- 230000008844 regulatory mechanism Effects 0.000 description 1
- 230000008263 repair mechanism Effects 0.000 description 1
- 230000001850 reproductive effect Effects 0.000 description 1
- 108091008146 restriction endonucleases Proteins 0.000 description 1
- 238000004366 reverse phase liquid chromatography Methods 0.000 description 1
- 238000003757 reverse transcription PCR Methods 0.000 description 1
- 108020004418 ribosomal RNA Proteins 0.000 description 1
- 102220010922 rs397507457 Human genes 0.000 description 1
- 210000003296 saliva Anatomy 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 210000002966 serum Anatomy 0.000 description 1
- 238000004904 shortening Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 230000003007 single stranded DNA break Effects 0.000 description 1
- 238000012868 site-directed mutagenesis technique Methods 0.000 description 1
- 238000001542 size-exclusion chromatography Methods 0.000 description 1
- 239000004055 small Interfering RNA Substances 0.000 description 1
- 229910000030 sodium bicarbonate Inorganic materials 0.000 description 1
- 235000017557 sodium bicarbonate Nutrition 0.000 description 1
- 229910000029 sodium carbonate Inorganic materials 0.000 description 1
- 239000001509 sodium citrate Substances 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 230000000392 somatic effect Effects 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 210000000278 spinal cord Anatomy 0.000 description 1
- 230000000087 stabilizing effect Effects 0.000 description 1
- 238000010561 standard procedure Methods 0.000 description 1
- 150000003431 steroids Chemical class 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 229960005322 streptomycin Drugs 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
- 230000010741 sumoylation Effects 0.000 description 1
- 230000001629 suppression Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 238000004114 suspension culture Methods 0.000 description 1
- 208000024891 symptom Diseases 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 235000013616 tea Nutrition 0.000 description 1
- 230000002123 temporal effect Effects 0.000 description 1
- MPLHNVLQVRSVEE-UHFFFAOYSA-N texas red Chemical compound [O-]S(=O)(=O)C1=CC(S(Cl)(=O)=O)=CC=C1C(C1=CC=2CCCN3CCCC(C=23)=C1O1)=C2C1=C(CCC1)C3=[N+]1CCCC3=C2 MPLHNVLQVRSVEE-UHFFFAOYSA-N 0.000 description 1
- 238000005382 thermal cycling Methods 0.000 description 1
- 230000008646 thermal stress Effects 0.000 description 1
- 230000005026 transcription initiation Effects 0.000 description 1
- 230000005030 transcription termination Effects 0.000 description 1
- 230000010474 transient expression Effects 0.000 description 1
- 230000005945 translocation Effects 0.000 description 1
- 201000007905 transthyretin amyloidosis Diseases 0.000 description 1
- QORWJWZARLRLPR-UHFFFAOYSA-H tricalcium bis(phosphate) Chemical compound [Ca+2].[Ca+2].[Ca+2].[O-]P([O-])([O-])=O.[O-]P([O-])([O-])=O QORWJWZARLRLPR-UHFFFAOYSA-H 0.000 description 1
- 230000001960 triggered effect Effects 0.000 description 1
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 description 1
- HRXKRNGNAMMEHJ-UHFFFAOYSA-K trisodium citrate Chemical compound [Na+].[Na+].[Na+].[O-]C(=O)CC(O)(CC([O-])=O)C([O-])=O HRXKRNGNAMMEHJ-UHFFFAOYSA-K 0.000 description 1
- 229940038773 trisodium citrate Drugs 0.000 description 1
- 230000010415 tropism Effects 0.000 description 1
- 238000001419 two-dimensional polyacrylamide gel electrophoresis Methods 0.000 description 1
- 230000034512 ubiquitination Effects 0.000 description 1
- 238000010798 ubiquitination Methods 0.000 description 1
- 238000002604 ultrasonography Methods 0.000 description 1
- 241000701447 unidentified baculovirus Species 0.000 description 1
- 238000011144 upstream manufacturing Methods 0.000 description 1
- 210000002700 urine Anatomy 0.000 description 1
- 230000002792 vascular Effects 0.000 description 1
- 239000003981 vehicle Substances 0.000 description 1
- 210000003501 vero cell Anatomy 0.000 description 1
- 230000009385 viral infection Effects 0.000 description 1
- 239000000277 virosome Substances 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 208000012137 von Willebrand disease (hereditary or acquired) Diseases 0.000 description 1
- 239000011534 wash buffer Substances 0.000 description 1
- 230000003442 weekly effect Effects 0.000 description 1
- 230000036266 weeks of gestation Effects 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/102—Mutagenizing nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
- C12N9/22—Ribonucleases [RNase]; Deoxyribonucleases [DNase]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6818—Hybridisation assays characterised by the detection means involving interaction of two or more labels, e.g. resonant energy transfer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2310/00—Structure or type of the nucleic acid
- C12N2310/10—Type of nucleic acid
- C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPR]
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Microbiology (AREA)
- General Health & Medical Sciences (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Crystallography & Structural Chemistry (AREA)
- Medicinal Chemistry (AREA)
- Analytical Chemistry (AREA)
- Immunology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (6)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201962885483P | 2019-08-12 | 2019-08-12 | |
| US62/885,483 | 2019-08-12 | ||
| US201962901875P | 2019-09-18 | 2019-09-18 | |
| US62/901,875 | 2019-09-18 | ||
| US202063030088P | 2020-05-26 | 2020-05-26 | |
| US63/030,088 | 2020-05-26 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| TW202120688A true TW202120688A (zh) | 2021-06-01 |
Family
ID=72243198
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| TW109127218A TW202120688A (zh) | 2019-08-12 | 2020-08-11 | Rna導引之核酸酶及其活性片段及變體以及使用方法 |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US20220364074A1 (enExample) |
| EP (1) | EP4013864A1 (enExample) |
| JP (2) | JP7640527B2 (enExample) |
| KR (1) | KR20220062289A (enExample) |
| CN (2) | CN114641568B (enExample) |
| AU (1) | AU2020329912A1 (enExample) |
| BR (1) | BR112022002695A2 (enExample) |
| CA (1) | CA3147783A1 (enExample) |
| IL (1) | IL290562A (enExample) |
| MX (1) | MX2022001849A (enExample) |
| TW (1) | TW202120688A (enExample) |
| WO (1) | WO2021030344A1 (enExample) |
Families Citing this family (23)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US9228207B2 (en) | 2013-09-06 | 2016-01-05 | President And Fellows Of Harvard College | Switchable gRNAs comprising aptamers |
| AU2015330699B2 (en) | 2014-10-10 | 2021-12-02 | Editas Medicine, Inc. | Compositions and methods for promoting homology directed repair |
| US12157760B2 (en) | 2018-05-23 | 2024-12-03 | The Broad Institute, Inc. | Base editors and uses thereof |
| US12522807B2 (en) | 2018-07-09 | 2026-01-13 | The Broad Institute, Inc. | RNA programmable epigenetic RNA modifiers and uses thereof |
| WO2020092453A1 (en) | 2018-10-29 | 2020-05-07 | The Broad Institute, Inc. | Nucleobase editors comprising geocas9 and uses thereof |
| KR20210149686A (ko) * | 2018-12-27 | 2021-12-09 | 라이프에디트 테라퓨틱스, 인크. | 유전자 편집에 유용한 폴리펩티드 및 사용 방법 |
| US12351837B2 (en) | 2019-01-23 | 2025-07-08 | The Broad Institute, Inc. | Supernegatively charged proteins and uses thereof |
| WO2020191233A1 (en) | 2019-03-19 | 2020-09-24 | The Broad Institute, Inc. | Methods and compositions for editing nucleotide sequences |
| US12473543B2 (en) | 2019-04-17 | 2025-11-18 | The Broad Institute, Inc. | Adenine base editors with reduced off-target effects |
| US12435330B2 (en) | 2019-10-10 | 2025-10-07 | The Broad Institute, Inc. | Methods and compositions for prime editing RNA |
| TW202208626A (zh) | 2020-04-24 | 2022-03-01 | 美商生命編輯公司 | Rna引導核酸酶及其活性片段與變體,以及使用方法 |
| IL297761A (en) | 2020-05-08 | 2022-12-01 | Broad Inst Inc | Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence |
| AU2021300378A1 (en) | 2020-07-01 | 2023-01-19 | Elevatebio Technologies, Inc. | Compositions and methods for cellular reprogramming using circular RNA |
| US20240247242A1 (en) | 2020-07-15 | 2024-07-25 | LifeEDIT Therapeutics, Inc. | Uracil stabilizing proteins and active fragments and variants thereof and methods of use |
| MX2023002848A (es) | 2020-09-11 | 2023-03-22 | Lifeedit Therapeutics Inc | Enzimas modificadoras del acido desoxirribonucleico (adn) y fragmentos activos y sus variantes y metodos de uso. |
| US20240301385A1 (en) * | 2021-03-22 | 2024-09-12 | LifeEDIT Therapeutics, Inc. | Dna modifying enzymes and active fragments and variants thereof and methods of use |
| AU2022262623A1 (en) * | 2021-04-22 | 2023-11-30 | Emendobio Inc. | NOVEL OMNI 117, 140, 150-158, 160-165, 167-177, 180-188, 191-198, 200, 201, 203, 205-209, 211-217, 219, 220, 222, 223, 226, 227, 229, 231-236, 238-245, 247, 250, 254, 256, 257, 260 and 262 CRISPR NUCLEASES |
| WO2024077267A1 (en) * | 2022-10-07 | 2024-04-11 | The Broad Institute, Inc. | Prime editing methods and compositions for treating triplet repeat disorders |
| KR20250126155A (ko) | 2022-11-04 | 2025-08-22 | 라이프 에디트 테라퓨틱스, 인크. | 내부 삽입 부위를 갖는 진화된 아데닌 데아미나제 및 rna-가이드 뉴클레아제 융합 단백질 및 사용 방법 |
| WO2024127370A1 (en) * | 2022-12-16 | 2024-06-20 | LifeEDIT Therapeutics, Inc. | Guide rnas that target trac gene and methods of use |
| WO2024178397A2 (en) | 2023-02-24 | 2024-08-29 | Elevatebio Technologies, Inc. | Modified immune effector cells and methods of use |
| WO2025174908A1 (en) * | 2024-02-12 | 2025-08-21 | Life Edit Therapeutics, Inc. | Novel rna-guided nucleases and proteins for polymerase editing |
| WO2026003754A1 (en) | 2024-06-25 | 2026-01-02 | Life Edit Therapeutics, Inc. | Novel reverse transcriptases and uses thereof |
Family Cites Families (75)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4217344A (en) | 1976-06-23 | 1980-08-12 | L'oreal | Compositions containing aqueous dispersions of lipid spheres |
| US4196265A (en) | 1977-06-15 | 1980-04-01 | The Wistar Institute | Method of producing antibodies |
| US4235871A (en) | 1978-02-24 | 1980-11-25 | Papahadjopoulos Demetrios P | Method of encapsulating biologically active materials in lipid vesicles |
| US4186183A (en) | 1978-03-29 | 1980-01-29 | The United States Of America As Represented By The Secretary Of The Army | Liposome carriers in chemotherapy of leishmaniasis |
| US4261975A (en) | 1979-09-19 | 1981-04-14 | Merck & Co., Inc. | Viral liposome particle |
| US4485054A (en) | 1982-10-04 | 1984-11-27 | Lipoderm Pharmaceuticals Limited | Method of encapsulating biologically active materials in multilamellar lipid vesicles (MLV) |
| US4501728A (en) | 1983-01-06 | 1985-02-26 | Technology Unlimited, Inc. | Masking of liposomes from RES recognition |
| US5380831A (en) | 1986-04-04 | 1995-01-10 | Mycogen Plant Science, Inc. | Synthetic insecticidal crystal protein gene |
| US4897355A (en) | 1985-01-07 | 1990-01-30 | Syntex (U.S.A.) Inc. | N[ω,(ω-1)-dialkyloxy]- and N-[ω,(ω-1)-dialkenyloxy]-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
| US5049386A (en) | 1985-01-07 | 1991-09-17 | Syntex (U.S.A.) Inc. | N-ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)Alk-1-YL-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
| US4946787A (en) | 1985-01-07 | 1990-08-07 | Syntex (U.S.A.) Inc. | N-(ω,(ω-1)-dialkyloxy)- and N-(ω,(ω-1)-dialkenyloxy)-alk-1-yl-N,N,N-tetrasubstituted ammonium lipids and uses therefor |
| US4797368A (en) | 1985-03-15 | 1989-01-10 | The United States Of America As Represented By The Department Of Health And Human Services | Adeno-associated virus as eukaryotic expression vector |
| US5569597A (en) | 1985-05-13 | 1996-10-29 | Ciba Geigy Corp. | Methods of inserting viral DNA into plant material |
| US4774085A (en) | 1985-07-09 | 1988-09-27 | 501 Board of Regents, Univ. of Texas | Pharmaceutical administration systems containing a mixture of immunomodulators |
| US4853331A (en) | 1985-08-16 | 1989-08-01 | Mycogen Corporation | Cloning and expression of Bacillus thuringiensis toxin gene toxic to beetles of the order Coleoptera |
| US5268463A (en) | 1986-11-11 | 1993-12-07 | Jefferson Richard A | Plant promoter α-glucuronidase gene construct |
| US5608142A (en) | 1986-12-03 | 1997-03-04 | Agracetus, Inc. | Insecticidal cotton plants |
| US4837028A (en) | 1986-12-24 | 1989-06-06 | Liposome Technology, Inc. | Liposomes with enhanced circulation time |
| US5039523A (en) | 1988-10-27 | 1991-08-13 | Mycogen Corporation | Novel Bacillus thuringiensis isolate denoted B.t. PS81F, active against lepidopteran pests, and a gene encoding a lepidopteran-active toxin |
| EP0388186A1 (en) | 1989-03-17 | 1990-09-19 | E.I. Du Pont De Nemours And Company | External regulation of gene expression |
| ATE225853T1 (de) | 1990-04-12 | 2002-10-15 | Syngenta Participations Ag | Gewebe-spezifische promotoren |
| US5264618A (en) | 1990-04-19 | 1993-11-23 | Vical, Inc. | Cationic lipids for intracellular delivery of biologically active molecules |
| WO1991017424A1 (en) | 1990-05-03 | 1991-11-14 | Vical, Inc. | Intracellular delivery of biologically active substances by means of self-assembling lipid complexes |
| US5498830A (en) | 1990-06-18 | 1996-03-12 | Monsanto Company | Decreased oil content in plant seeds |
| CA2051562C (en) | 1990-10-12 | 2003-12-02 | Jewel M. Payne | Bacillus thuringiensis isolates active against dipteran pests |
| US5173414A (en) | 1990-10-30 | 1992-12-22 | Applied Immune Sciences, Inc. | Production of recombinant adeno-associated virus vectors |
| US5399680A (en) | 1991-05-22 | 1995-03-21 | The Salk Institute For Biological Studies | Rice chitinase promoter |
| US5604121A (en) | 1991-08-27 | 1997-02-18 | Agricultural Genetics Company Limited | Proteins with insecticidal properties against homopteran insects and their use in plant protection |
| TW261517B (enExample) | 1991-11-29 | 1995-11-01 | Mitsubishi Shozi Kk | |
| US5587308A (en) | 1992-06-02 | 1996-12-24 | The United States Of America As Represented By The Department Of Health & Human Services | Modified adeno-associated virus vector capable of expression from a novel promoter |
| US5789156A (en) | 1993-06-14 | 1998-08-04 | Basf Ag | Tetracycline-regulated transcriptional inhibitors |
| US5814618A (en) | 1993-06-14 | 1998-09-29 | Basf Aktiengesellschaft | Methods for regulating gene expression |
| US5605793A (en) | 1994-02-17 | 1997-02-25 | Affymax Technologies N.V. | Methods for in vitro recombination |
| US5837458A (en) | 1994-02-17 | 1998-11-17 | Maxygen, Inc. | Methods and compositions for cellular and metabolic engineering |
| US5608144A (en) | 1994-08-12 | 1997-03-04 | Dna Plant Technology Corp. | Plant group 2 promoters and uses thereof |
| US5659026A (en) | 1995-03-24 | 1997-08-19 | Pioneer Hi-Bred International | ALS3 promoter |
| US6072050A (en) | 1996-06-11 | 2000-06-06 | Pioneer Hi-Bred International, Inc. | Synthetic promoters |
| AR014072A1 (es) | 1998-02-26 | 2001-01-31 | Pioneer Hi Bred Int | Molecula de acido nucleico aislada que tiene una secuencia nucleotidica para un promotor que es capaz de iniciar una transcripcion constitutiva en unacelula de planta, construccion adn, vector, celula huesped, metodo para expresar en forma constitutiva una secuencia nucleotidica heteroloca en una |
| US6534261B1 (en) | 1999-01-12 | 2003-03-18 | Sangamo Biosciences, Inc. | Regulation of endogenous gene expression in cells using zinc finger proteins |
| US6555655B1 (en) | 1999-05-04 | 2003-04-29 | Monsanto Technology, Llc | Coleopteran-toxic polypeptide compositions and insect-resistant transgenic plants |
| US6593293B1 (en) | 1999-09-15 | 2003-07-15 | Monsanto Technology, Llc | Lepidopteran-active Bacillus thuringiensis δ-endotoxin compositions and methods of use |
| US20050183161A1 (en) | 2003-10-14 | 2005-08-18 | Athenix Corporation | AXMI-010, a delta-endotoxin gene and methods for its use |
| WO2005066202A2 (en) | 2003-12-22 | 2005-07-21 | E.I. Du Pont De Nemours And Company | Bacillus cry9 family members |
| AU2007260716B2 (en) | 2006-06-14 | 2013-05-02 | BASF Agricultural Solutions Seed US LLC | Axmi-031, axmi-039, axmi-040 and axmi-049, a family of delta-endotoxin genes and methods for their use |
| US8383604B2 (en) | 2008-09-15 | 2013-02-26 | Children's Medical Center Corporation | Modulation of BCL11A for treatment of hemoglobinopathies |
| ES2609332T3 (es) | 2009-07-02 | 2017-04-19 | Athenix Corporation | Gen pesticida AXMI-205 y métodos para su uso |
| WO2011084324A2 (en) | 2009-12-21 | 2011-07-14 | Pioneer Hi-Bred International, Inc. | Novel bacillus thuringiensis gene with lepidopteran activity |
| KR20160008655A (ko) | 2009-12-24 | 2016-01-22 | 제이엑스 닛코 닛세키 킨조쿠 가부시키가이샤 | 가돌리늄제 스퍼터링 타깃 및 동 타깃의 제조 방법 |
| WO2012024200A2 (en) | 2010-08-19 | 2012-02-23 | Pioneer Hi-Bred International, Inc. | Novel bacillus thuringiensis gene with lepidopteran activity |
| US9405700B2 (en) | 2010-11-04 | 2016-08-02 | Sonics, Inc. | Methods and apparatus for virtualization in an integrated circuit |
| AU2013266968B2 (en) * | 2012-05-25 | 2017-06-29 | Emmanuelle CHARPENTIER | Methods and compositions for RNA-directed target DNA modification and for RNA-directed modulation of transcription |
| EP3196301B1 (en) | 2012-07-11 | 2018-10-17 | Sangamo Therapeutics, Inc. | Methods and compositions for the treatment of monogenic diseases |
| CA2930877A1 (en) * | 2013-11-18 | 2015-05-21 | Crispr Therapeutics Ag | Crispr-cas system materials and methods |
| US20150165054A1 (en) | 2013-12-12 | 2015-06-18 | President And Fellows Of Harvard College | Methods for correcting caspase-9 point mutations |
| EP3186375A4 (en) | 2014-08-28 | 2019-03-13 | North Carolina State University | Novel CAS9 PROTEINS AND CHARACTERISTICS FOR DNA TARGETING AND GENOME EDITING |
| EP3245232B1 (en) * | 2015-01-12 | 2021-04-21 | The Regents of The University of California | Heterodimeric cas9 and methods of use thereof |
| AU2016263026A1 (en) * | 2015-05-15 | 2017-11-09 | Pioneer Hi-Bred International, Inc. | Guide RNA/Cas endonuclease systems |
| US9790490B2 (en) | 2015-06-18 | 2017-10-17 | The Broad Institute Inc. | CRISPR enzymes and systems |
| IL310721B2 (en) | 2015-10-23 | 2025-11-01 | Harvard College | Nucleobase editors and uses thereof |
| EP3426780A1 (en) * | 2016-03-11 | 2019-01-16 | Pioneer Hi-Bred International, Inc. | Novel cas9 systems and methods of use |
| EP3426778A1 (en) * | 2016-03-11 | 2019-01-16 | Pioneer Hi-Bred International, Inc. | Novel cas9 systems and methods of use |
| CN109843914B (zh) * | 2016-07-06 | 2024-03-15 | 沃泰克斯药物股份有限公司 | 用于治疗疼痛相关病症的材料和方法 |
| CN110214183A (zh) | 2016-08-03 | 2019-09-06 | 哈佛大学的校长及成员们 | 腺苷核碱基编辑器及其用途 |
| EP3538661A4 (en) * | 2016-11-14 | 2020-04-15 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | METHOD FOR BASIC PROCESSING IN PLANTS |
| US11542496B2 (en) * | 2017-03-10 | 2023-01-03 | President And Fellows Of Harvard College | Cytosine to guanine base editor |
| KR20240116572A (ko) * | 2017-03-23 | 2024-07-29 | 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 | 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제 |
| SG11201906297QA (en) * | 2017-03-24 | 2019-10-30 | Curevac Ag | Nucleic acids encoding crispr-associated proteins and uses thereof |
| US11345924B2 (en) * | 2017-05-11 | 2022-05-31 | Institute Of Genetics And Developmental Biology, Chinese Academy Of Sciences | Creation of herbicide resistant gene and use thereof |
| EP3630849A4 (en) * | 2017-05-25 | 2021-01-13 | The General Hospital Corporation | BIPARTITE BASIC EDITOR (BBE) AND TYPE II-C-CAS9 ZINC FINGER EDITOR ARCHITECTURES |
| WO2019036185A1 (en) * | 2017-08-15 | 2019-02-21 | The Regents Of The University Of California | COMPOSITIONS AND METHODS FOR IMPROVING GENOMIC SEQUENCE CORRECTION |
| US11649442B2 (en) * | 2017-09-08 | 2023-05-16 | The Regents Of The University Of California | RNA-guided endonuclease fusion polypeptides and methods of use thereof |
| CA3082450A1 (en) * | 2017-11-21 | 2019-05-31 | Crispr Therapeutics Ag | Materials and methods for treatment of autosomal dominant retinitis pigmentosa |
| CA3084185A1 (en) * | 2017-12-06 | 2019-06-13 | Generation Bio Co. | Gene editing using a modified closed-ended dna (cedna) |
| PL3802807T3 (pl) | 2018-06-05 | 2025-03-31 | Life Edit Therapeutics, Inc. | Nukleazy kierowane rna i ich aktywne fragmenty oraz warianty i sposoby ich zastosowania |
| CN109837328B (zh) * | 2018-09-20 | 2021-07-27 | 中国科学院动物研究所 | 核酸检测方法 |
-
2020
- 2020-08-11 WO PCT/US2020/045759 patent/WO2021030344A1/en not_active Ceased
- 2020-08-11 CA CA3147783A patent/CA3147783A1/en active Pending
- 2020-08-11 MX MX2022001849A patent/MX2022001849A/es unknown
- 2020-08-11 JP JP2022509175A patent/JP7640527B2/ja active Active
- 2020-08-11 BR BR112022002695A patent/BR112022002695A2/pt unknown
- 2020-08-11 AU AU2020329912A patent/AU2020329912A1/en active Pending
- 2020-08-11 KR KR1020227008114A patent/KR20220062289A/ko active Pending
- 2020-08-11 TW TW109127218A patent/TW202120688A/zh unknown
- 2020-08-11 CN CN202080068763.3A patent/CN114641568B/zh active Active
- 2020-08-11 CN CN202411155276.7A patent/CN118813654A/zh active Pending
- 2020-08-11 US US17/634,723 patent/US20220364074A1/en active Pending
- 2020-08-11 EP EP20761938.8A patent/EP4013864A1/en active Pending
-
2022
- 2022-02-13 IL IL290562A patent/IL290562A/en unknown
-
2025
- 2025-02-20 JP JP2025025892A patent/JP2025086912A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| EP4013864A1 (en) | 2022-06-22 |
| CN114641568B (zh) | 2024-09-06 |
| US20220364074A1 (en) | 2022-11-17 |
| KR20220062289A (ko) | 2022-05-16 |
| MX2022001849A (es) | 2022-03-11 |
| CN118813654A (zh) | 2024-10-22 |
| CN114641568A (zh) | 2022-06-17 |
| JP7640527B2 (ja) | 2025-03-05 |
| BR112022002695A2 (pt) | 2022-08-23 |
| JP2022545385A (ja) | 2022-10-27 |
| CA3147783A1 (en) | 2021-02-18 |
| IL290562A (en) | 2022-04-01 |
| AU2020329912A1 (en) | 2022-03-24 |
| WO2021030344A1 (en) | 2021-02-18 |
| JP2025086912A (ja) | 2025-06-09 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| US12338455B2 (en) | RNA-guided nucleases and active fragments and variants thereof and methods of use | |
| US12188018B2 (en) | Polypeptides useful for gene editing and methods of use | |
| TW202120688A (zh) | Rna導引之核酸酶及其活性片段及變體以及使用方法 | |
| TWI922428B (zh) | Rna導引之核酸酶及其使用方法 | |
| EA049684B1 (ru) | Рнк-направляемые нуклеазы, их активные фрагменты и варианты, а также способы применения | |
| EA047491B1 (ru) | Рнк-направляемые нуклеазы, их активные фрагменты и варианты, а также способы применения | |
| HK40063040B (en) | Polypeptides useful for gene editing and methods of use | |
| HK40063040A (en) | Polypeptides useful for gene editing and methods of use | |
| EA048415B1 (ru) | Полипептиды, применимые для редактирования генов, и способы их применения | |
| HK40050306B (en) | Rna-guided nucleases and active fragments and variants thereof and methods of use | |
| HK40050306A (en) | Rna-guided nucleases and active fragments and variants thereof and methods of use | |
| EA052783B1 (ru) | Полипептиды, применимые для редактирования генов, и способы их применения | |
| EA046542B1 (ru) | Рнк-управляемые нуклеазы и активные фрагменты, и их варианты и способы применения |