TW201601774A - Composition containing natural plant extracts, external skin preparation comprising the same, cosmetic preparation comprising the same and pharmaceutical preparation comprising the same - Google Patents

Abstract

The present invention relates to a composition containing, as active ingredients, a Cymbidium kanran extract; and at least one selected from the group consisting of a green tea extract, a Camellia japonica extract, a Bletilla striata extract, and an Opuntia Coccinellifera extract, an external skin preparation including the composition, a cosmetic preparation including the composition, and a pharmaceutical preparation including the composition.

Description

Composition containing natural plant extract, external preparation for skin containing the same, cosmetic preparation containing the same, and pharmaceutical preparation containing the same

The present invention relates to a composition containing a natural plant extract as an active ingredient, a skin external preparation containing the same, a cosmetic preparation containing the same, a cosmetic preparation containing the same, and a pharmaceutical preparation containing the same. More specifically, the present invention relates to a Cymbidium kanran extract and an extract of green tea extract, Camellia japonica extract, Bletilla striata extract, and Opuntia Coccinellifera extract. At least one extract selected from the group is used as an active ingredient to have an antioxidant and anti-inflammatory composition.

Inflammatory skin disease includes atopic dermatitis, contact dermatitis, and lipid leakage Dermatitis, acne, and similar diseases.

In general, the mechanism or stimulatory response or inflammation involves various cytokines released by human keratinocytes or Langerhans cells in response to foreign substances. Cytokines, the accelerators of the human immune system, are essential for skin irritation or local inflammation (J Appl Toxicol 16: 65-70, 1996). In fact, it has been reported that in the case of stimulating contact dermatitis, interleukin-6 (IL-6), interleukin-1 (IL-1) or tumor necrosis factor alpha (tumor necrosis factor alpha, Increased TNF-α) (Contact Dermatitis 35: 355-60, December 1996).

Conventionally, antihistamine drugs, vitamin ointments or adrenocortical drugs have been used to treat the inflammatory skin diseases, but most of them have a temporary effect and in many cases even have side effects.

Therefore, there is a need to develop a composition which is effective for treating diseases associated with oxidation or inflammation which occur in the skin and which contains natural plant extracts as an active ingredient and which does not cause side effects as compared with hormone preparations.

Previous literature Patent literature

(Patent Document 1)

Korean Patent Licensing Public Notice No. 10-2013-0031988 (April 1, 2013)

An object of the present invention is to provide a composition comprising a plurality of natural plant extracts as an active ingredient to have antioxidant and anti-inflammatory effects, and comprising the group A skin external preparation, a cosmetic preparation, and a pharmaceutical preparation.

The present invention provides a composition comprising a Hanlan extract and at least one extract selected from the group consisting of green tea extract, camellia extract, white peony extract, and nopal cactus extract as an active ingredient.

The present invention also provides a skin external preparation comprising the composition.

The invention also provides a cosmetic preparation comprising the composition.

The invention also provides a pharmaceutical formulation comprising the composition.

The role of the invention

The composition of the present invention contains at least two kinds of natural plant extracts as active ingredients and thus has a relatively good antioxidant activity for removing active oxygen and good skin alleviation as compared with a composition containing a single natural plant extract as an active ingredient. Inflammation has an anti-inflammatory effect.

1 is a view showing the results of TNF-α evaluation of respective compositions according to Comparative Example 1 to Comparative Example 5 and Examples 1 to 5 in Experimental Example 1.

2 is a view showing the results of IL-6 evaluation of the respective compositions according to Comparative Example 1 to Comparative Example 5 and Examples 1 to 5 in Experimental Example 2.

The present invention provides a composition comprising a Hanlan extract and at least one extract selected from the group consisting of green tea extract, camellia extract, white peony extract, and nopal cactus extract as an active ingredient.

In the present invention, the term "Hanlan" refers to a plant of the orchid family of the Orchidaceae species.

In the present invention, green tea refers to a product made from the buds or leaves of Camellia Sinensis plants by exposing the oxidase present in the tea leaves to heat or steam to activate the enzyme.

In the present invention, camellia refers to a camellia plant which is a tea tree species.

In the present invention, white peony refers to a white peony plant of the genus Orchidaceae.

In the present invention, the nopal cactus refers to a nopal cactus plant of the cactus family.

The Hanlan extract, the green tea extract, the camellia extract, the white peony extract, and the nopal cactus extract can be prepared according to respective preparation methods known in the related art, that is, using known solvents under known temperature and pressure conditions. Preferably, each extract is extracted by an extraction solvent selected from the group consisting of water, anhydrous or aqueous lower alcohol having 1 to 4 carbon atoms, acetone, ethyl acetate, butyl acetate, glycerin, 1, 3-butanediol, 1,2-propanediol, and 1,3-propanediol.

The extract of Hanlan can be extracted from the whole plant of Hanlan. The white peony extract can also be extracted from the whole plant of the white peony.

The green tea extract can be extracted from the leaves of the tea plant and the camellia extract can be extracted from the leaves of the camellia plant.

The nopal cactus extract can be extracted from the fruit of the carmine plant.

In one embodiment of the invention, the composition comprises Hanlan extract and green tea extract as active ingredients.

Due to the mixture of Hanlan extract and green tea extract, the use of at least two natural plant extracts as a constituent of the active ingredient can increase its antioxidant activity. Strongly maximized.

In one embodiment of the invention, the composition comprises Hanlan extract and Camellia extract.

Due to the mixture of the Hanlan extract and the Camellia extract, the use of at least two natural plant extracts as a constituent of the active ingredient maximizes the enhancement of the anti-inflammatory action in the TNF-α inhibited form.

In one embodiment of the invention, the composition comprises a Hanlan extract and a white peony extract.

Due to the mixture of the Hanlan extract and the white peony extract, the composition can relatively maximize the enhancement of the anti-inflammatory effect in the TNF-α inhibition state compared to the composition containing the single natural plant extract. .

In one embodiment of the invention, the composition comprises a Hanlan extract and a nopal cactus extract.

Due to the mixture of the extract containing Hanlan and the extract of the nopal cactus, the composition can relatively enhance the anti-inflammatory effect in the IL-6 inhibited state as compared with the composition containing the single natural plant extract. Chemical.

In one embodiment of the invention, the composition comprises Hanlan extract and green tea extract.

Due to the mixture of Hanlan extract and green tea extract, the composition containing two natural plant extracts as active ingredients maximizes TNF-α and IL-6 inhibition to maximize anti-inflammatory effects.

In one embodiment of the present invention, the composition contains green tea extract, Hanlan extract, camellia extract, white peony extract, and nopal cactus extract as active ingredients.

By using a green tea extract, a Hanlan extract, a camellia extract, a white peony extract, and a nopal cactus extract as a mixture of active ingredients, the composition can maximize anti-inflammatory and anti-oxidant effects.

In one embodiment of the invention, the composition comprises 0.0001% by weight or more than 0.0001% by weight and 10% by weight or less of 10% by weight, relative to the total weight of the composition, more specifically, 0.01 % by weight or more than 0.01% by weight and 5.0% by weight or less than 5.0% by weight of Hanlan extract. 0.0001% by weight or more than 0.0001% by weight of the extract of Hanlan extract can provide antioxidant and anti-inflammatory effects from Hanlan extract. 10% by weight or less by 10% by weight of the Hanlan extract content is more effective by relatively enhancing the antioxidant and anti-inflammatory effects from the Hanlan extract relative to the increment.

In one embodiment of the invention, the composition comprises 0.0001% by weight or more than 0.0001% by weight and 90% by weight or less than 90% by weight, based on the total weight of the composition, of green tea extract, more specifically 0.01 % by weight or more than 0.01% by weight and 90% by weight or less than 90% by weight of green tea extract. A green tea extract content of 0.0001% by weight or more than 0.0001% by weight provides an antioxidant and anti-inflammatory action from the green tea extract. 90% by weight or less by weight of the green tea extract content is more effective by relatively enhancing the antioxidant and anti-inflammatory effects from the green tea extract relative to the increase in content.

In one embodiment of the invention, the composition comprises 0.0001% by weight or more than 0.0001% by weight and 90% by weight or less than 90% by weight of the camellia extract relative to the total weight of the composition, more specifically, 0.01 % by weight or more than 0.01% by weight and 90% by weight or less than 90% by weight of Camellia extract. 0.0001% by weight or more than 0.0001% by weight of Camellia extract content can be provided from Camellia Take anti-oxidation and anti-inflammatory effects. The content of the camellia extract of 90% by weight or less is more effective by relatively enhancing the antioxidant and anti-inflammatory effects from the extract of Camellia relative to the increase in the content.

In one embodiment of the present invention, the composition comprises 0.0001% by weight or more than 0.0001% by weight and 10% by weight or less by weight based on the total weight of the composition of the chalk extract, more specifically, 0.01. % by weight or more than 0.01% by weight and 5% by weight or less than 5% by weight of the chalk extract. 0.0001% by weight or more than 0.0001% by weight of the chalk extract content provides antioxidant and anti-inflammatory effects from the extract of Radix Paeoniae Alba. The content of chalk extract of 10% by weight or less by 10% by weight is more effective by relatively enhancing the antioxidant and anti-inflammatory effects from the extract of Radix Paeoniae Alba relative to the increase in content.

In one embodiment of the invention, the composition comprises 0.0001% by weight or more than 0.0001% by weight and 90% by weight or less than 90% by weight of the nopal cactus extract relative to the total weight of the composition, more specifically, 0.01% by weight or more than 0.01% by weight and 90% by weight or less than 90% by weight of the nopal cactus extract. The 0.0001% by weight or more than 0.0001% by weight of the agaric cactus extract content provides antioxidant and anti-inflammatory effects from the nopal cactus extract. The 90% by weight or less than 90% by weight of the agaric cactus extract content is more effective by relatively enhancing the antioxidant and anti-inflammatory effects from the nopal cactus extract relative to the increase in content.

In one embodiment of the invention, the composition is used as an antioxidant composition. The antioxidant composition uses at least two extracts as active ingredients to eliminate or inhibit free radicals in the skin.

In one embodiment of the invention, the composition is used as an anti-inflammatory composition. The anti-inflammatory composition uses at least two extracts as active ingredients to inhibit or reduce Interleukin-6 (IL-6), interleukin-1 (IL-1) or tumor necrosis factor alpha (TNF-α).

The present invention provides a skin external preparation comprising the above-listed compositions.

The external preparation for skin broadly refers to all preparations applied to the outside of the skin and includes any preparation administered by absorption through the skin, that is, any transdermal preparation.

In one embodiment of the invention, the skin comprises skin around the eye.

In one embodiment of the present invention, specific formulations of the transdermal formulation include, but are not limited to, powder formulations for external use, tablet formulations for external use, liquid formulations for external use, patches, microneedles , ointment or suppository.

The present invention provides a cosmetic comprising the compositions listed above.

The cosmetic product can be formulated using a cosmetically or dermatologically acceptable medium or matrix. The cosmetic refers to any kind of cosmetic formulation suitable for topical application, including, for example, solutions, gels, solids, pasty anhydrous products, oil-in-water emulsions, suspensions, microemulsions, microcapsules, microparticles, Ionic (liposome) or nonionic vesical dispersant, cream, toner, lotion, powder, ointment, spray or concealer. The cosmetic product may also be provided in the form of a foam composition or an aerosol composition additionally comprising a compressed propellant. The cosmetic preparation can be prepared according to methods known in the related art.

In addition, the cosmetic may contain a lipid substance, an organic solvent, a solubilizer, a concentrate, a gelling agent, a softener, an antioxidant, a suspending agent, a stabilizer, a foaming agent, a flavoring agent, a surfactant, water, ions or Nonionic emulsifiers, fillers, chelating agents, chelating agents, preservatives, vitamins, blockers, wetting agents, essential oils, dyes, pigments, hydrophilic or lipophilic activators, lipid vesicles, or generally used in cosmetics or Any other adjuvant in the field of dermatology, such as other commonly used in cosmetics Minute. Adjuvants can be used in a cosmetically or dermatologically acceptable amount.

The present invention provides a pharmaceutical preparation comprising the composition listed above.

The composition may additionally contain a pharmaceutical adjuvant such as a preservative, a stabilizer, a water-dispersible powder, an emulsification accelerator, a salt for controlling osmotic pressure, and/or a buffer, and the like, and is suitable for treatment and formulated into various kinds according to a general method. Oral or parenteral dosage forms of other substances.

Examples of oral dosage forms include tablets, pills, hard or soft capsules, liquids, suspensions, emulsions, syrups, granules and the like which contain diluents in addition to the active ingredient (for example, lactose, dextrose, sucrose, nectar Sugar alcohols, sorbitol, cellulose, and glycine), lubricants (such as ceria, talc, stearic acid and its magnesium or calcium salts, and polyethylene glycol). Tablets may further contain binders such as magnesium aluminum silicate, starch paste, gelatin, tragacanth, methylcellulose, sodium carboxymethylcellulose, and polyvinylpyrrolidone, and in some cases, pharmaceutical additives For example, a disintegrating agent (for example, starch, agar, alginic acid or a sodium salt thereof), an adsorbent, a coloring agent, a flavoring agent, a sweetener, and the like. Tablets can be prepared by a general method involving mixing, granulating or coating. In addition, a representative example of a parenteral dosage form is a formulation for injection, preferably an equal proportion of an aqueous solution or suspension.

In the following, the construction and function of the present invention will be described in detail with reference to the examples and examples, which are provided to provide a better understanding of the invention and are not intended to limit the invention. category.

<Comparative Example 1> Preparation of Hanlan extract

950 g of purified water was added to 50 g of the whole plant of Hanlan, followed by extraction with hot water at 80 ° C for 12 hours. The extract thus obtained was filtered through a 1 micron filter and concentrated with an evaporator until the amount of the extracted liquid reached 3.5 grams. To prepare a sample. 20 micrograms of Hanlan extract was used in each of Experimental Examples 1 to 3 below.

<Comparative Example 2> Preparation of Angelica Extract

A whole plant of 20 g of white peony was mixed with 80 g of a solvent mixture of purified water and glycerin (3:7 by weight) and extracted at 25 ° C for 3 days. The extract thus obtained was filtered through a 1 micron filter to prepare a white peony extract. 20 micrograms of chalk extract was used in each of the following Experimental Example 1 to Experimental Example 3.

<Comparative Example 3> Preparation of Camellia Extract

The procedure was carried out in the same manner as described in Comparative Example 1, except that 20 gram of camellia leaves were used instead of the whole plant of Hanlan. 20 μg of Camellia extract was used in each of Experimental Examples 1 to 3 below.

<Comparative Example 4> Preparation of nopal cactus extract

The procedure was carried out in the same manner as described in Comparative Example 1, except that the whole plant of the Hanlan was replaced with 20 grams of the fruit of the nopal cactus. 20 micrograms of the nopal cactus extract was used in each of the following Experimental Example 1 to Experimental Example 3.

<Comparative Example 5> Preparation of green tea extract

The procedure was carried out in the same manner as described in Comparative Example 1, except that 20 g of green tea leaves were used instead of the whole plant of Hanlan. 20 μg of green tea extract was used in each of Experimental Examples 1 to 3 below.

<Example 1> Preparation of mixed extract of Hanlan and Angelica

10 μg of the Hanlan extract from Comparative Example 1 was mixed with 10 μg of the extract of Cretaceous from Comparative Example 2 to prepare a mixture of Hanlan and Angelica. Take things.

<Example 2> Preparation of mixed extract of Hanlan and Camellia

Ten micrograms of the Hanlan extract from Comparative Example 1 was mixed with 10 μg of the camellia extract from Comparative Example 3 to prepare a mixed extract of Hanlan and Camellia.

<Example 3> Preparation of mixed extract of Hanlan and nopal cactus

Ten micrograms of the Hanlan extract from Comparative Example 1 was mixed with 10 micrograms of the nopal cactus extract from Comparative Example 4 to prepare a mixed extract of Hanlan and Nopal Cactus.

<Example 4> Preparation of mixed extract of Hanlan and green tea

Ten micrograms of the Hanlan extract from Comparative Example 1 was mixed with 10 μg of the green tea extract from Comparative Example 5 to prepare a mixed extract of Hanlan and green tea.

<Example 5> Preparation of mixed extracts of Hanlan, Angelica, Camellia, Nopal Cactus, and Green Tea

4 μg of the Hanlan extract from Comparative Example 1, 4 μg of the extract of Cretaceous from Comparative Example 2, 4 μg of Camellia Extract from Comparative Example 3, 4 μg of the Nopal Cactus Extract from Comparative Example 4, and 4 μg from comparison The green tea extract of Example 5 was mixed together to prepare a mixed extract of Hanlan, Angelica, Camellia, Nopal Cactus, and Green Tea.

<Experimental Example 1> Evaluation of free radicals, DPPH

To evaluate the antioxidant effects of the individual extracts prepared in Examples 1 through 5, via the free radical 1,3-diphenyl-2-picryl hydrazyl, DPPH) reduces the change in absorbance A comparative assessment of DPPH oxidation inhibition was performed to assess antioxidant capacity. In other words, the extracts obtained in Examples 1 to 5 and Comparative Examples 1 to 5 were measured for the decrease in the absorbance caused by the inhibition of DPPH oxidation with respect to the control group, and thus the absorbance was the control absorbance. A concentration of 50% or less is considered to be an effective antioxidant concentration. Regarding the effective antioxidant concentration, each of the control and individual extracts was separately measured three times. The ratio of the average effective antioxidant concentration and the average effective antioxidant concentration of the corresponding extract to the effective antioxidant concentration of the control group is shown in Table 1.

As shown in Table 1, when Comparative Example 2 was compared with Example 1, Comparative Example 3 and Example 2, Comparative Example 4 and Example 3, and Comparative Example 5 and Example 4, Han Lan extract was combined with another extract. Examples 1 to 4 were far superior in antioxidant action to Comparative Example 2 to Comparative Example 5 containing a single extract as an active ingredient.

In particular, Example 4 of a green tea extract containing a combination with a Hanlan extract is compared to a green tea extract as a single active ingredient in terms of antioxidant activity. 5 is much better.

In addition, Example 5, which contains Hanlan extract, white peony extract, camellia extract, nopal cactus extract, and green tea extract as various active ingredients, is most effective in antioxidant action.

<Experimental Example 2> Evaluation of TNF-α inhibition

To evaluate the TNF-α inhibition of the respective extracts prepared in Examples 1 to 5, experiments were performed on human keratinocyte cell line HaCaT cells (provided by Korean Cell Line Bank).

HaCaT cells were seeded in a 48-well culture dish at a concentration of 1 × 10 ⁴ cells/well, and cultured in DMEM medium (Biowhittaker, Cat. No. 12-604F) containing 10% serum for 24 hours. Further incubation was carried out for 24 hours in serum-free medium. The total protein content in the cultured cells was measured three times and averaged. The cultured cells were washed once with PBS, and 1 μg/ml of LPS was added to the medium with 100 μl of PBS in addition to the control group. After removing the PBS, each sample of the extracts prepared in Examples 1 to 5 and Comparative Examples 1 to 5 was added at a concentration of 10 μm in a concentration of 10 μm in an amount of 500 μl in addition to the control group and the untreated group. Put in each hole. Each medium was collected within 6 hours after the treatment and the amount of TNF-α was measured three times using a TNF-α ELISA assay kit (BD Biosciences, catalog number 555212). The TNF-[alpha] measurements were averaged and the results are presented in Table 2 and plotted in the graph of Figure 1.

As shown in Table 2 and the graph of FIG. 1, when Comparative Example 2 was compared with Example 1, Comparative Example 3 and Example 2, Comparative Example 4 and Example 3, and Comparative Example 5 and Example 4, it was contained with another extract. Examples 1 to 4 of the combined Hanlan extract were far superior to the comparative example 2 to the comparative example 5 containing a single extract as an active ingredient in terms of anti-inflammatory action.

Specifically, Example 1 containing a white peony extract in combination with a Hanlan extract is much better in anti-inflammatory effect than Comparative Example 2 containing a white peony extract as a single active ingredient.

Further, in the composition containing the two natural plant extracts as an active ingredient, Example 4 containing the green tea extract in combination with the Hanlan extract is the best in terms of the anti-inflammatory action in the aspect of TNF-α inhibition.

Further, Example 5 containing Hanlan extract, white peony extract, camellia extract, nopal cactus extract, and green tea extract as various active ingredients is most effective in terms of anti-inflammatory action.

<Experimental Example 3> Evaluation of IL-6 inhibition

The procedure was performed in the same manner as described in Experimental Example 2, but instead the amount of IL-6 in each medium was measured instead of TNF-α using a TNF-α ELISA assay kit (BD Biosciences, Cat. No. 555212). Three times to evaluate IL-6 inhibition use. The measurement results are presented in Table 3 and plotted in the graph of Figure 2.

As shown in the graphs of Table 3 and FIG. 2, when Comparative Example 2 was compared with Example 1, Comparative Example 3 and Example 2, Comparative Example 4 and Example 3, and Comparative Example 5 and Example 4, it was contained with another extract. Examples 1 to 4 of the combined Hanlan extract were far superior to the Comparative Example 2 to Comparative Example 5 in which the single extract was used as the active ingredient in terms of the anti-inflammatory action in the IL-6 inhibitory aspect.

In particular, Example 3 of the nopal cactus extract containing the combination with the Hanlan extract was much better in the anti-inflammatory effect in the aspect of IL-6 inhibition than Comparative Example 4 containing the nopalate extract as a single active ingredient.

Further, in the composition containing the two natural plant extracts as an active ingredient, Example 4 containing the green tea extract in combination with the Hanlan extract is the best in terms of the anti-inflammatory action in the aspect of IL-6 inhibition.

In addition, Example 5 containing Hanlan extract, Angelica extract, Camellia extract, Nopal cactus extract, and green tea extract as various active ingredients The anti-inflammatory effect in the aspect of IL-6 inhibition is most effective.

Claims (9)

A cosmetic composition comprising Cymbidium kanran extract and selected from the group consisting of green tea extract, Camellia japonica extract, Bletilla striata extract, and Opuntia Coccinellifera At least one extract is used as an active ingredient. The cosmetic composition according to claim 1, wherein the Hanlan extract is extracted from a whole plant of Hanlan. The cosmetic composition according to claim 1, wherein the white peony extract is extracted from a whole plant of white peony. The cosmetic composition of claim 1, wherein the green tea extract is extracted from the leaves of Camellia Sinensis . The cosmetic composition of claim 1, wherein the camellia extract is extracted from the leaves of camellia. The cosmetic composition of claim 1, wherein the nopal cactus extract is extracted from the fruit of the nopal cactus. The cosmetic composition according to claim 1, wherein the cosmetic composition contains: 0.0001% by weight to 10% by weight of the Hanlan extract; 0.0001% by weight to the total weight of the cosmetic composition. 90% by weight of the green tea extract; 0.0001% by weight to 90% by weight of the camellia extract; 0.0001% by weight to 10% by weight of the chalk extract; or 0.0001% by weight to 90% by weight of the nopal cactus extract. The cosmetic composition according to claim 1, wherein the composition is used for an antioxidant composition. The cosmetic composition of claim 1, wherein the composition is for an anti-inflammatory composition.