TW201343190A - Combinations of skin care ingredients apply to moisturizing and anti-wrinkle - Google Patents

Abstract

The present invention relates to a combination of two different types of hyaluronic acid for moisturizing and anti-wrinkle of skin. The first type of hyaluronic acid is an unenveloped hyaluronic acid for moisturizing the skin. The second type is a liposome enveloped hyaluronic acid that can penetrate into epidermis and dermis for anti-wrinkle. The present invention can moisturize and anti-wrinkle for skin.

Description

Skin care combination ingredients for moisturizing and wrinkle removal

The invention relates to a skin care maintenance composition for applying moisturizing and wrinkle removal.

As we age, human skin ages, becomes dry and wrinkles. In recent years, with the popularization of air conditioners, the indoor air is more dry, and the outdoor air pollutants are increased, and the degree of dryness and aging of the skin is more obvious. In order to solve this problem, a wide variety of cosmetics are introduced on the market.

Hyaluronic acid is a mucopolysaccharide found in the dermis and found in body fluids, bovine eyeballs, cockscombs, and animal tissues. Hyaluronic acid in the skin is present in the extracellular matrix between cells, and hyaluronic acid is contained in both the epidermis and the dermis. The dermis layer is thick, the extracellular space between cells is large, and the amount of hyaluronic acid is large.

Hyaluronic acid has excellent water-holding capacity, it can absorb a thousand times its own weight of water, so it can keep the skin moist, avoid drying and wrinkles. Hyaluronic acid has a film-forming property, which can make the skin lock water smooth. Because hyaluronic acid is a component of human skin, it is excellent in skin-friendly properties, and it is not easy to have skin discomfort or allergies. It is widely used in skin care products.

The production technology of hyaluronic acid is mainly divided into two categories, the first one is the extraction method using animal tissue as raw material, and the second type is microbial fermentation method. The hyaluronic acid obtained by these two methods is consistent in chemical nature and molecular structure, and the difference is mainly in the difference in relative molecular weight. For example, hyaluronic acid extracted from the rooster crown has a molecular weight of several million Daltons up to six million Daltons; and microbial extracted hyaluronic acid is between tens of thousands and three million Daltons. Because the hyaluronic acid molecule is too large, it is difficult to penetrate the surface of the skin, and generally only stays outside the stratum corneum of the skin to exert a moisturizing function.

The Republic of China Patent Publication No. 201,043,259 discloses a skin moisturizing complex comprising hyaluronic acid and a method of producing the same, which has a moisturizing effect and has no wrinkle removing effect.

Since hyaluronic acid is an intrinsic biological substance of the skin, exogenous hyaluronic acid is a supplement to the endogenous hyaluronic acid of the skin. If hyaluronic acid can penetrate into the epidermis of the skin, it will replenish and increase the hyaluronic acid content in the skin, and prevent skin aging and reduce wrinkles.

Liposome has a long history of drug use, but its application in the cosmetics industry has only begun to develop in the last decade. Since the molecular structure of the liposome itself is very similar to the molecular structure of the skin, the use of the liposome to carry the active ingredient can more effectively deliver the desired ingredients to the inner layer of the skin.

The present invention provides a skin care maintenance composition for application to moisturizing and wrinkle removal.

The present invention provides a cosmetic composition for skin moisturizing and wrinkle which contains two types of hyaluronic acid. The first is unenveloped Hyaluronic Acid, with an average molecular weight ranging from several thousand Daltons to three million Daltons; the second is a liposome coated hyaluronic acid. (Liposome Enveloped Hyaluronic Acid). The non-coated type sodium hyaluronate has a large molecular weight and is difficult to penetrate the skin and reach the dermis layer, so it provides a function of locking water and moisturizing on the surface of the skin. The hyaluronic acid-coated hyaluronic acid can easily pass through the epidermis and reach the dermis layer, which can achieve the effect of reducing the fine lines.

Combined with the moisturizing function of sodium hyaluronate in the outer layer of the skin, and the removal effect of the microcapsule-coated sodium hyaluronate salt on the dermis layer, it can achieve the dual functions of moisturizing and wrinkle-removing skin care products.

The experimental examples and examples are now described, and the experimental examples and examples are for illustrative purposes only and are not intended to limit the scope of the invention.

<Example 1-4> Preparation of non-coated aqueous hyaluronic acid solution:

Example 1, weighing pure water into a 1 liter beaker, weighing an appropriate amount of preservative into the water, stirring and fully soluble, then weighing hyaluronic acid (average molecular weight 2 million Daltons), stirring fully dissolved, the appearance is transparent and clear state. Examples 2-4, the proportion of hyaluronic acid was changed, and the rest of the procedure was the same as in Experimental Example 1.

<Example 5-8>

Formulated with hyaluronic acid in a lipid-coated form.

Example 5, weighing 1.00 g of soybean lecithin, 0.03 g of vitamin E acetate, 1.00 g of 0.2 wt% aqueous hyaluronic acid solution (average molecular weight of 2 million Daltons), placed in a 20 ml scintillation bottle, magnetized stirring, capping, The water bath was maintained at 70 ° C for 2 hours for hydration. Use a probe-type ultrasonic wave (MISONIX, Sonicator 3000) to oscillate for 3 minutes. The particle size was measured by a particle size analyzer (Sanern), and the average liposome particle size was 105 nm.

Examples 6-8 were prepared by changing the ratio of aqueous hyaluronic acid solution to a hyaluronic acid-coated hyaluronic acid, and the remaining parameters were the same as in Example 5.

Examples 9-16 are inventive operations of cold emulsions.

<Example 9>

The composition shown in Table 3 was adjusted to an emulsion by the following method. The skin irritation test of the lotion on the hand and the performance test of the face skin were carried out.

Pour the component (3) emulsifier and the ingredients (1) (2) (9) oil into the emulsification tank at room temperature and mix well. Add the ingredients (10) pure water and emulsify evenly. Continue to add ingredients (4) (5) (6) (7) (8), open the stirring and emulsifying device, in a yellowish white emulsion state, viscosity 8,100 cps.

Skin irritation test

The emulsion prepared by the composition of Table 3 was subjected to a irritating test of the skin of the hand. Twenty women were selected, aged 20 to 60 years, 10 of whom were 20-40 years old, and 10 were 41-60 years old. Patients without skin allergies and skin diseases were tested. Apply a proper amount of emulsion to a small round dish (8 mm in diameter; 0.8 mm in depth) on the Finn Chamber patch, and then attach the Finn Chamber patch to the flexor skin of the upper arm. After direct contact with the skin for 48 hours, observe the skin condition. The Finn Chamber patch is applied to the flexor side of the upper arm. After 48 hours of direct contact with the skin, the patch is removed and the skin is washed and rested for 1, 2, and 3 hours. None of the 20 subjects had any redness, swelling, itching and other discomforts, and the subject did not feel any discomfort during the whole process, showing that the skin care products of this formula had no irritating reaction.

Skin Efficacy Test

The emulsion prepared by the composition of Table 3 was subjected to a skin efficacy test. Twenty women were selected, aged 20 to 60 years, 10 of whom were 20-40 years old, and 10 were 41-60 years old. Patients without skin allergies and skin diseases were tested. Daily morning and evening, continuous use for 6 weeks, measured at 0, 1, 2, 3, 4, 5, and 6 weeks, the subject was washed before the test and at 20 ± 1 ° C, 50 ± Rest for 30 minutes in a 5% RH environment, and then perform skin analysis and analysis through various non-invasive skin test instruments. The obtained data were statistically analyzed to evaluate the water content of the stratum corneum of the test skin for the face. Skin water dispersion loss, wrinkle rate, skin elasticity, gross pores, skin roughness, etc. Improve the efficacy and extent of skin texture.

Results: Table 4 and Figure 1 show the results of the second week, the fourth week, and the sixth week. After 2 weeks of use, the skin moisturizing and wrinkle-reducing effect, after 6 weeks of use, the cuticle moisture content increased by 10.1%; transepidermal water loss rate decreased by 4.1%; wrinkle rate decreased by 4.9%; skin elasticity increased by 8.3% The number of enlarged pores was reduced by 4.5%; the skin roughness was reduced by 6.7%. This result shows that the skin moisturizing and wrinkle-removing effect can be achieved by using a non-coated hyaluronic acid and a micro-ester coated hyaluronic acid as a raw material for skin care products.

<Example 10-18>

Examples 10-18, the formulation is shown in Table 5, and the operation is as in Example 9. Pour the component (6) emulsifier and the ingredients (1) (2) (3) (4) (5) oil into the emulsification tank at room temperature, and mix well. Add the ingredients (21) pure water and emulsify evenly. The ingredients (7) to (20) are continuously added, and the mixture is stirred and emulsified uniformly.

<Example 17-19>

Examples 17-19 are inventive operations of hot emulsions, and the formulations are shown in Table 6. Ingredients (1) to (5) oils and (6) (7) emulsifiers are placed in an emulsification tank, stirred and heated to maintain 72-75 ° C; ingredients (21) purified water is heated to 72-75 ° C, Into the emulsifying tank. Emulsify and stir evenly. Cool down to below 40 ° C, add ingredients (8) ~ (20) in sequence, stir and emulsifie evenly.

<Example 20-22>

The method 20 is a hyaluronic acid-based gel, and the components (3)(4)(5)(11)(13) are uniformly stirred and gelatinized, which is the moisturizing and wrinkle-removing skin care product composition of the invention. .

Examples 21 and 22 are gels thickened with Sanxian gum and hydroxyethyl cellulose. The ingredients (12) are first mixed in a uniform state with the formula (1). Add the formula (2) and dissolve evenly. Then, (13) pure water is added as a clear liquid, and the formulas (3) to (11) are successively added, and finally, a uniform gel state is obtained by a high speed mixer.

<Example 23-24>

Examples 23-24 are inventive compositions of lotions. Ingredient (10) essence is first mixed with component (1) in a uniform state. Add the formula (2) and dissolve evenly. Then add (11) purified water, and add formula (3) ~ (9) one after another, and mix well.

Fig. 2 is a view showing the operation of the moisturizing and wrinkle-removing skin care composition of the present invention. According to the composition provided by the present invention, the skin moisturizing and wrinkle removing effect can be achieved.

"Fig. 1", the skin skin test results of the second, fourth, and sixth weeks of use of the moisturizing and wrinkle-removing skin care composition of the present invention

"Fig. 2" is a description of the operation components of the skin care combination for moisturizing and wrinkle removal of the present invention.

Claims (8)

The invention provides a composition for skin care products applied to moisturizing and wrinkle removal, which comprises two types of hyaluronic acid. The first is a non-coated hyaluronic acid with an average molecular weight of between 10,000 Daltons and 3 million Daltons; the second is a hyaluronic acid coated hyaluronic acid. According to the combination of the invention patent item 1, hyaluronic acid refers to the sodium salt of hyaluronic acid. According to the combination of the invention patent item 1, the uncoated type hyaluronic acid can be first prepared into a 0.01 to 1.2% aqueous solution, preferably 0.2 to 1.0% aqueous solution, and most preferably 0.2 to 0.5% aqueous solution. According to the combination component of the invention patent item 1, the skin moisturizing and wrinkle-removing cosmetic composition can be adjusted into a dosage form of an emulsion, a cream, an essence, a gel, a lotion, and the like. According to the combination of the invention patent item 1, it has a moisturizing and wrinkle removing effect. According to the fifth invention patent, the moisturizing effect refers to increasing the moisture content of the stratum corneum and reducing the transepidermal water loss rate. According to the fifth aspect of the invention patent, the wrinkle effect refers to reducing the wrinkle rate, increasing the skin elasticity, reducing the number of enlarged pores, and reducing the skin roughness. According to the first aspect of the invention patent, the cosmetic component for moisturizing and wrinkle-removing further comprises apple cell culture extract, seaweed extract, natural astaxanthin, etc., to increase moisturizing and wrinkle-removing effects.