201208571 六、發明說明. 【發明所屬之技術領域】 尤其發生於香奪、 之葉斑 本發明為有關芭蕉科植物 病之防除方法。 【先前技術】 屬於巴癌科巴焦屬之植物，尤其香1為食用果實之 品種群之總稱，乃指其果實而言。該植物主要栽= 國、臺灣等之東亞；菲律賓、印尼、奏國、：裁培於中 南亞；印度等西亞：巴^、厄瓜多、、南等之東 Μ瓜夕爾、哥斯大黎 西哥、瓜地馬拉、哥儉比亞、秘魯等令南美"曰： 向非專之非洲，也施行大規模農聿栽 培而整年栽培。於這此各國祛+立 ^ t 权呆戰 界各地輸出。栽培，所收獲之香“向世 於香蕉栽培’含有莖部之根部在地下、高伸地上部 之如莖之部分乃稱偽莖，實際上為葉勒重疊幾層者，由 偽莖之前端大大伸出長橢圓形之葉(葉身)，更由偽莖之前 端生出果軸（PedUneU)，於其先形成含有果指（finger)、果 房（utricle)之全房（bunch)，而以全房生育香黨。於香筹之 收成後，此偽莖部（母株）乃於地上部2m前後之處切斷。 7此時將由地下之根部新出現之新偽莖部（子株）同樣地 繁殖，或將新偽莖部（子株）分株繁殖來反覆香蕉之收成。 對於香焦之栽培’將葉（機能葉）留下幾片頗為重要，因由 最後之抽出葉（開花前）至收穫有3個月，故有必要於開花 期有將機能葉留下至丨12〜13片左右。若機能葉之片數 為不充分之情形，則於栽培途中砍下植物。 201208571 就香蕉栽培之主要病害而言有葉斑病、黄色之黄葉 斑病（Yellow Sigatoka)及黑色之黑葉斑病（Black Sigatoka) ° 黄葉斑病為起因於香蕉生球腔菌（Myc〇sphaerella mu si col a)、黑葉斑病為起因於斐濟球腔菌 (Mycosphaerella fijiensis)之病害，尤其黑葉斑病為發生 於高溫多濕時，使香蕉之葉變成黑色且阻礙光合成而使 收成量減半之病害。葉斑病之潛伏期間為3週至2個月 其後’羅患之香無之初期病徵為於由頂葉起4〜5片下 之古葉之葉背出現小斑點’不久沿葉脈產生黃色之條斑 ，逐漸地病斑擴大而變成暗褐色。若被害加重，則幾乎 所有之葉枯死而下垂’只留下中心葉之1〜2片。這些病 害之防除為由監視香煮之葉部開始，依感染之最幼葉之 位置與感染程度來判斷防除之要否。 一旦確認葉斑病發生，則多用錳鋅乃浦（Manc〇zeb) 等有機硫系殺菌劑、四氣二氰苯（Chlorothalonil)等有機 乳系殺菌劑.、嘴霉胺（p i r i m e t h a n i 1)等°密π定系殺菌劑、三 得芬（Tridemorph)、芬普福（Fenpropimorf)等嗎啉系殺菌 劑、待克利（difenoconazole)、比多農（Bitertanol)、普克 利（Propiconazole)、敗環。圭（Epoxiconazole)等三唾系殺菌 齊J 0比。坐鍵函 S曰（Pyraclostrobin)、喊菌 S旨（azoxystrobin) 荨甲氧基丙稀酸醋系（s t r 〇 b i 1 u r i η)殺菌劑，這些殺菌劑乃 以10日至20日之間隔散布數種。 又近年已知本身不呈示殺菌活性或弱，但將對病害 之抵抗性誘導於植物之稱抵抗性誘導劑（SAR劑）之一群 201208571 化合物作為各種作物之病害防除劑有用，而噻啩基（一般 名.tiadinil)及異嗟菌胺（一般名：is〇tiann)、阿拉酸式 本-S -甲基（一般名：acibenz〇iar-S-methyl)、環丙醯菌胺（ 身又名.carpropamid)、撲殺熱（一般名：probenazole)、 柳酉文（salicylic acid)、二氣於驗酸（dichloronicotinic acid) 等為前述抵抗性誘導劑。更將與噻醯菌胺類似化合物之 2’，4’-二曱氧基-4-環丙基_ι，2,3·嗟二。坐-5-叛酿胺（以下 也稱化合物A」）’也作為植物病害防除劑為有用之公知 化合物（參照例如專利文獻1 )，但被考慮為抵抗性誘導劑 。這些抵抗性誘導劑之中已知異噻菌胺對香蕉之黑葉斑 病以散布處理而呈示效果（參照例如專利文獻2)。 上述以一般名或化學名表示之化合物為公知化合物 ’可購入市售品或依公知文獻記載之方法製造（參照例如 非專利文獻1)。 [先前技術文獻] [專利文獻] [專利文獻1 ]特開2 0 0 7 - 9 9 7 4 9號公報 [專利文獻2]國際公開20 1 〇/〇3 7482號小冊 [非專利文獻] [非專利文獻 l]The Pesticide Manual 14th Edition (British Crop Production Council) 【發明内容】 [發明欲解決之課題] 如上所述’葉斑病防除對香蕉栽培雖為最重要課題， 但於習知技術因施行多數回藥劑處理，故其勞力膨大，更 201208571 =耐性：之出現，使防除更困難。故殷求為將防除困 :之葉斑病有效率地防除之環境負荷少，且有使散布筚劑 篁之減低可能等優點，而省力之新穎防除方法。 *本發明者等為解決上述課題而反覆致力研究之結果 :藉將香蕉之葉斑病防除劑於香蕉之偽莖部(葉鞘部 以注入益等注入，則發規台b胳士十中> j發現此將本病害有效率防除之防除 :二終於完成本發明之防除方法。也即 明 之於偽莖部注入藥液之防除方法，可將發生於香雀2 ^病有效率地峰之外，也可防除發生於新由地 =之新偽莖部(子株)之葉斑病，比葉斑病防除劑： =理可達成顯著長期之防除效果，且可 …藥劑散布者之負擔，終於完成本發明。 也即本發明為有關至少以下之各發明： 體之(二於葉斑病發生或預測發生之色煮科植物之植物 以防卜t部（葉勒部）將有效量葉斑病防除劑注入處理， :’生於前述以、科植物之植物體之葉斑病 &業斑病之防除方法。 之前(if、斑病防除劑為抵抗性誘導劑或浸透性殺菌劑 之則迷⑴之葉斑病之防除方法。 (3)抵抗性誘導劑為读啡 a A(2，，4，_: ^ A 噻哜基、異噻菌胺、化合物 拉酸式苯-s::-4:環丙基_1，2,3-噻二唑-5·羧醯胺)、阿 鹼酸之前述(2):葉"丙醯菌胺、撲殺熱、柳酸、二氣終 t )之葉斑病之防除方法。 前述誘導劑為㈣基、異°塞菌胺或化合物A之 中任一項之葉斑病之防除方法。 201208571 (5) 浸透性殺菌劑為甲氧基丙烯酸酯系（str〇bilurin) 殺菌劑之前述（2)之葉斑病之防除方法。 (6) 甲氧基丙稀酸酯系殺菌劑為„密菌酯 (azoxystrobin)、苯氧菌胺（Metominostrobin)、啶氧菌酿 (Picoxystrobin)、吡唑醚菌酯（pyraclostrobin)、敗。密菌醋 、二氧。密菌醋或將鍵菌胺之前述（2)或（5)之葉斑病之防除 方法。 (7) 浸透性殺菌劑為唑系殺菌劑之前述（2)之葉斑病 之防除方法。 (8) 。坐系殺菌劑為得克利（tebuconazole)、待克利、普 克利（propiconazole)、克座（cyproconazole)、氟娃。坐 (Flusilazole)、腈菌唾（myCi〇butanil)、護汰芬（Flutriafol) 、二°坐醇（triadimenol)、菲克利（hexaconazole)、戊菌。全） 、烯。坐醇（Diniconazole)、三泰芬）、種菌。坐（ipC〇naz〇ie) 、漠克座（Bromuconazole)、娃坐（Simeconazole)、亞胺 σ坐（imibenconazole)、丙硫菌嗤（pr〇thi〇c〇nazole)或抑霉 嗤（Imazalil)之前述（7)之葉斑病之防除方法。 (9) 爸焦科植物為香蕉之前述（丨）之葉斑病之防除方 法。 (10) 葉斑病為起因於Mycosphaerella musicola之葉 斑病之前述（1)〜（9)之任一項之葉斑病之防除方法。 (11) 葉斑病為起因於MyC〇Spharella fijiensis之葉斑 病之則述（1)〜（9)之任一項之葉斑病之防除方法。 201208571 (1 2)於發生或預測發生葉斑病之香蕉母株之偽莖部（ 葉鞘部）將有效量之葉斑病防除劑注入處理，以防除於前 述香蒸母株之香蕉子株發生葉斑病為特徵之香蕉子株葉 斑病之防除方法。 (1 3)葉斑病防除劑為抵抗性誘導劑或浸透性殺菌劑 之則述（12)之葉斑病之防除方法。 (1 4)抵抗性誘導劑為噻畊基、異噻菌胺、化合物a、 阿拉酸式苯-S-甲基、環丙醯菌胺、撲殺熱、柳酸、二氯 知驗酸之前述（1 3 )之葉斑病之防除方法。 (1 5)抵杬性誘導劑為噻啩基、異噻菌胺或化合物a之 前述（1 4)之葉斑病之防除方法。 (1 6)/文透性殺菌劑為曱氧基丙稀酸酷系殺菌劑之前 述（14)之葉斑病之防除方法。 (1 7)曱氧基丙烯酸酯系殺菌劑為嘧菌酯、苯氧菌胺 咬氧菌 S曰（Picoxystrobin)、°比 〇坐趟菌醋（pyraci〇str〇bin) 、氟嘲菌酯、二氧嘧菌酯或肟醚菌胺之前述（17)之葉斑 病之防除方法。 (1 8 )>叉透性权痛劑為。坐糸殺菌劑之前述（1 3 )之葉斑 病之防除方法。 (1 9 ) °坐糸殺函劑為得克利（t e b u c ο n a ζ ο 1 e)、待克利、 普克利（Propiconazole)、克座、氟硅唑、腈菌唑、護汰 刀 一 0上醇、菲克利、戊卤°坐、稀口坐醇（Diniconazole)、 三泰芬、種菌唑、溴克座、硅氟唑、亞胺唑、丙硫菌唑 或抑霉唑之前述（1 8 )之葉斑病之防除方法。 201208571 [發明之效果] 本發明之葉斑 於香蕉發生或預測 部）將有效量葉斑病 斑病防除方法為比 便之處理方法、長 性菌也呈示優異之 莖部長出之新葉鞘 發明之葉斑病防除 可能減低處理藥量 0 【實施方式】 [實施發明之形態] 本發明可使用 透性殺菌劑、抵抗· :tiadinil)、異噻 g 拉酸式苯-S-曱基（. 醯菌胺（一般名： probenazole)、相 p (Dichloronicotinic 或異噻菌胺較佳。 名：azoxystrobin) 吡唑醚菌酯（一 (Metominostrobin) 病防除方法為以芭蕉科植物，尤其對 發生葉斑病之植物體之偽莖部（葉鞘 防除劑注入處理為特徵。本發明之葉 習知之葉斑病防除藥劑之散布處理簡 期提示顯著之防除效果、對於藥劑财 防除效果’且具有對於新由地下之根 部（子株）防止葉斑病發病之效果。本 方法更可能削減藥劑之處理回數，而 ’也為可減低對環境負荷之防除方法 之葉斑病防除劑為抵抗性誘導劑或浸 1生誘導齊;丨可使用例如噻醯菌胺（一般名 i胺（一般名：i s 〇 t i a n i 1)、化合物A、阿 一般名：acibenzolar-S-methyl)、環丙 Carpropamid)、撲殺熱（一般名： 酸（salicylic acid)、二氯於驗酸 acid)等，尤其以噻醯菌胺、化合物A 浸透性殺菌劑可使用例如嘧菌酯（一般 、三氟敏（一般名：trifloxystrobin)、 般名：pyraclostrobin)、苯氧菌胺 、啶氧菌酯（一般名：picoxystrobin) 201208571 、氟^痛菌醋（一般名：fluoxastrobin)、二氧略菌S旨（一般 名：dimoxystrobin)、月亏鱗菌胺（一般名：orysastrobin) 等曱氧基丙烯酸醋系（s t r 〇 b i 1 u r i η )殺菌劑、得克利（一般名 :tebuconazole)、敗環0圭（一般名：epoxiconazole)、待克 利（一般名：difenoconazole) ' 普克利（一般名： propiconazole)、克座（一般名：cyproconazole)、氟石圭口坐（ 一般名：flusilazole)、滅特座（一般名：metconazole)、 腈菌嗤（一般名：myclobutanil)、四克利（一般名： tetraconazole)、氟啥 0坐（一般名：fliuquinconazole) ' 護 汰芬（一般名：flutriafol)、三°坐醇（一般名：triadimenol) 、菲克利（一般名：hexaconazole)、戊菌唾（一般名： penconazole)、比多農（一般名：bitertanol)、滅菌嗤（一 般名：triticonazole)、氰苯 °坐（一般名：fenbuconazole) 、稀嗤醇（一般名：diniconazole)、三泰芬（一般名： triadimefon)、種菌0坐（一般名：ipconazole)、漠克座（一 般名：bromuconazole)、娃氟°坐（一般名：simeconazole) 、亞胺唑（一般名：i m i b e n c ο n a ζ ο 1 e)、丙硫菌。坐（一般名： prothioconazole)、撲克拉（一般名：prochloraz)、抑霉 〇坐 (一般名：i m a z a 1 i 1)、π惡咪°坐（ο X ρ 〇 c ο n a ζ ο 1 e )等唾系殺菌劑 ，但本發明可使用之葉斑病防除劑不限於這些有效成分 。這些藥劑有市售，容易由市場取得。又化合物A可依專 利文獻1記載之方法製造。 本發明之葉斑病之防除方法可將以上述化合物為有 效成分之市售液狀藥劑於香蕉等植物體之葉賴部以注射 -10- 201208571 器等注入器等注入處理來施行。又以化合物A為有效成 分之藥劑可將化合物A於適當之惰性載體與界面活性劑 ，必要時與輔助劑一起，以適當比例配合來溶解，作成 適宜之劑型，例如懸浮劑、乳懸浮劑、乳劑、液劑等製· 劑來使用。 葉斑病之防除劑1 00質量份中之有效成分之添加量 可由0.1質量份〜50質量份之範圍適宜選擇來使用，較 佳為1質量份〜50質量份之範圍。 本發明之防除方法所使用之惰性載體可使用液體或 固體之惰性載體，於向葉鞘部注入方法以液體載體較佳 ，能作為液體載體之材料，除其本身具有溶劑能力之外 ，可選自雖不具有溶劑能力，但有助於輔助劑使有效成 分化合物分散者，如此液體載體可為例如水、醇類（例如 曱醇、乙醇、異丙醇、丁醇、乙二醇等）、酮類（例如丙 酮、曱乙酮、甲基丁酮、二異丁酮、環己酮等）、醚類（ 例如乙醚、二崎烧、溶纖素、二丙、四氫°夫α南等）、脂 肪族烴類（例如煤油、礦油等）、芳香族烴類（例如苯、曱 苯、二曱苯、溶劑油、烷基萘等）、_化烴類（例如二氣 乙烷、氣仿、四氣化碳等）、酯類（例如乙酸乙酯、酞酸 二異丙酯、酞酸二丁酯、酞酸二辛酯等）、醯胺類（例如 二曱基曱醯胺、二乙基甲醯胺、二甲基乙醯胺等）、腈類 (例如乙腈等）、二曱亞颯類等、這些可以單獨或2種以 上之混合物之形使用。 其他之輔助劑可_舉出如下例示之代表性輔助劑，這 些輔助劑乃依目的而使用，可單獨或於某種情形併用2 -11 - 201208571 種以上之輔助劑’又於某種情形也可完全不使用輔助劑 能。為了有效成分化合物之乳化、分散、可溶化及/或濕 潤之目的，可使用界面活性劑，可舉出例如聚氧乙烯烷 基鍵、聚氧乙烯烷芳基醚、聚氧乙烯高級脂肪酸酯、聚 氧乙烯樹脂酸酯、聚氧乙烯山梨聚糖單十二酸酯、聚氧 乙稀山梨聚糖單油酸醋、烧芳基續酸鹽、蔡續酸縮合物 、木質磺酸鹽、高級醇硫酸酯等界面活性劑 '。懸浮性製 品之解膠劑可使用例如萘磺酸縮合物、縮合磷酸鹽等輔 助劑。消泡劑可使用例如聚矽氧烷油等輔助劑。 於本發明防除方法可防除葉斑病之芭蕉科之植物可 例示例如葛羅米謝爾（Gros Michel)、嘉雲臺（Cavendish) 、洞窟嘉雲臺（Dwarf Cavendish)、洞窟支那（Dwarf Chinese)、艾諾（Enano)、克支拉（Caturra)、巨人嘉雲臺 (Giant Cavendish)、克藍艾諾（Gran Enano)、大耐因 (Grande Naine)、威廉混種（Williams Hybrid)、巴蕾 (Valery)、羅勃絲（Robust)、波友（P〇y〇)、拉康坦（Lacatan) 、蒙特克里斯特（Monte cristo)、波特倫（Bout rond)等爸 蕉科植物，但不限於這些芭蕉科植物。尤其較佳為一般 作為食用來栽培之包括巨人嘉雲臺、洞窟嘉雲臺、嘉雲 臺、普藍天、紅香蕉、蘋果香蕉、猿香蕉、島香蕉等之 全部香蕉類。 於本發明之防除方法對象之芭蕉科植物，也包括就 除草劑耐性基因、殺蟲性害蟲抵抗性基因、抗病原性物 質産生基因、油糧成分改質或胺基酸含量增強形質等之 有用形質，施行基因轉換之基因改組體（GMO)之同植物。 -12- 201208571 於本％明之方法為了防除芭蕉科植物之葉斑病，防 除劑可就此，或以水等適宜稀釋，或以懸浮之形將葉斑 病防除有效量於於發生或預測發生葉斑病之香f、之植物 體ΐ ί Ϊ部’可使用注射器等注入器來注入。注入部位 及藥直右香蕉之果實為成長期或香蕉果實之收穫後可 於由殘留之偽莖部之地上部之地表起3〇cm〜lm左右之 任意處將所定藥量注入，以此處理可防除發生於成長中 香焦之葉斑病’若為收穫後，則也可防除發生由根莖部 長出處理’不只可防除母株本身之葉斑病，也可防 生於子株之葉斑病。 本發明之香蕉葉斑病防除劑可製劑成通常之劑型， 例如乳劑、水和劑、顆粒水和劑、可浮 劑型來使用’其處理量乃依有效成分之配合比例= 條件、製劑形態、處理時期、處理方法、處理場所等而 異？於通常之大規模農業栽培尤其常使用之乳劑之情 形每A頃之香蕉之栽培株數以1 500株〜2000株左右 栽培，於-般之藥劑散布為將所定藥量之藥劑以每公頃 0.4卜31散布’故相當於每株之藥量。依本 發明之防除方法，可於香f、之葉鞠部將稀釋為所定藥量 之藥液依每株0.2ml〜2nU之藥量以例如注射器等注入偽 莖部。此換算為有效成分量’則為香f'之植物體每株 60mg〜40〇mg之範圍，但於本發明之量不限於此。依本 發明之防除方法，可減低藥劑使用量，與由使用者散布 之情形相異，使用者不暴露於藥液（―般散布之情形卜 可更安全地防除葉斑病。 -13- 201208571 於本發明之葉斑病防除方法，可將上述殺菌劑以外 之農園藝用殺菌劑或農園藝用殺蟲劑適宜混合來使用， 如此將農園藝用殺菌劑或農園藝用殺蟲劑之代表性化合 物例示如下，但前述農園藝用殺菌劑或農園藝用殺蟲劑 不限於這些。農園藝用殺菌劑可為例如亞賜圃 (isoprothiolane)、.三環。坐（tricyclazole)、嘴菌西旨 (azoxystrobin)、〇比。坐謎鹵酉旨（pyraclostrobin)、普克.利 (propiconazole)、有機銅（coPPer-8-quinolinolate)、苯氧 菌胺（Metominostrobin)、環丙醯菌胺（carpropamid)、四 氣苯醜（fthalide)、嘉賜黴素（kasugamycin)、百快隆 (pyroquilon)、撲殺熱（probenazole)、阿拉酸式苯-S-曱基 (acibenzolar-S- methyl)、福多寧（flutolanil)、滅普寧 (mepronil)、福拉比（furametpyr)、賓克隆（pencycuron)、 賽 It 滅（thifluzamide)、維利黴素（validamycin)、達滅淨 (diclomezine)、。密菌腙（ferimzone)、雙胍辛鹽（guazatine) 、腈菌。坐（myclobutanil)、菲克利（hexaconazole)、待克利 (difenoconazole)、比多農（bitertanol)、 氟環唑（epoxiconazole)、三泰芬（triadimefon) (triadimefon)、撲克拉（prochloraz)、芬普福 (fenpropimorph)、三得芬（tridemorph)、滅達樂（metalaxyl) 、鹽基性鹽化銅（copper oxychloride)、鹽基性硫酸銅 (basic copper sulfate)、錳鋅乃浦（mancozeb)、甲基鋅乃 浦（propineb)、福美锌（Ziram)、秋蘭姆（thiuranl)、霜脲氰 (cymoxanil)、四氣異苯（chl〇rothalonil)、待克利 -14- 201208571 (difenoconazole)、蓋普丹（captan)、保利黴素（polyoxiii) 、依普同（iprodione)、撲滅寧（procymidone)、免賴得 (benomyl)、甲基硫菌靈（thiophanate-methyl)、多菌靈 (carbendazim)、福赛得（fosetyl-aluminium)、噁霉靈 (hymexazol)、歐索林酸（oxolinic acid)、咯菌腈 (fludiox.onil)、滅派林（mepanipyrim)、賽普洛（cyprodinil) 、0密霉胺（pyrimethanil)、曱基立枯填（tolclofos-methyl) 、氰菌胺（fenoxanil)等農園藝用殺菌劑混合使用。 農園藝用殺蟲劑可為例如免扶克（一般名： benfuracarb)、丁基加保扶（一般名：carbosulfan)、PHC( 一般名：propoxur)、吱線威（一般名：furathiocarb)、 納乃得（一般名：methomyl)、NAC(—般名：carbaryl)、 免敵克（一般名：bendiocarb)、棉鈴威（一般名：alanycarb) 、益達敍（一般名·· imidacloprid)、尼藤。比藍（一般名： nitenpyram)、亞滅培（一般名：acetamiprid)、賽速安（一 般名·· thiamethoxam)、克分（一般名：clothianidin)、達 特南（一般名：dinotefuran)、加護松（一般名：prop.aphos) 、乙拌礙（一般名：disulfoton)、地亞農（一般名：diazinon) 、三氣松（一般名：trichlorfon)、芬殺松（一般名：fenthion) 、歐殺松（一般名：acephate)、 加福松（一般名：isoxathion)、亞芬松（一般名： isofenphos)、甲基毒死碑（一般名：chlorpyrifos-methyl) 、殺填威（一般名：chlorfenvinphos)、大滅松（一般名： dimethoate)、大福松（一般名：fonofos)、必芬松（一般名 :pyridafenthion)、亞素靈（一般名：monocrotophos) ' 培 -15- 201208571 丹（一般名· cartap)、硫賜安（一般名：thiocyclam)、免 速達（一般名：bensultap) '乙氰菊酯（一般名·· cycloprothrin)、依芬寧（一般名：etofenprox)、矽護芬（ 一般名：silafluofen)、七氟菊酯（一般名·· tefiuthrin)、 布芬淨（一般名：buprofezin)、氟芬隆（一般名： flufenoxuron)、 賽滅淨（一般名：cyromazine)、祿芬隆（一般名： lufenuron)、得芬諾（一般名：tebufenozide)、曱氧蟲醯肼 (一般名：methoxyfenozide)、可芬諾（一般名： chromafenozide)、歐殺滅（一般名：oxamyl)、白克松（一 般名：pyraclofos)、福赛絕（一般名：fosthiazate)、芬普 滿（一般名：fenpyroximate)、芬普尼（一般名：fipronil) 、安殺蕃（一般名：endosulfan)、聚乙盤（一般名： metaldehyde) » 因滅 >、丁（一般名：emamectine-benzoate) ' 賜諾殺（一般名：spinosad)、克凡派（一般名：chlorfenapyr) ，因得克（一般名：indoxacarb)等混合使用。 [實施例] 以下例示本發明之代表性處方例及試驗例，但本發明 不限於這些。處方例或試驗例中，所謂部乃示重量份。 處方例1. 本發明之葉斑病防除劑之有效成分 5 0份 二曱苯 40份 聚氧乙烯壬基苯基醚與烷苯基磺酸鈣 之混合物 1 0份 將以上均勻混合溶解成乳劑。 -16 - 201208571 處方例2. 30份 5份 5份 2份 58份 本發明之葉斑病防除劑之有效成分 Sorpol3105(東邦化學工業製） 丙二醇201208571 VI. Description of the invention. [Technical field to which the invention pertains] The leaf spot which occurs particularly in the scent of the stalk. The present invention relates to a method for controlling plant diseases of the genus Musa. [Prior Art] A plant belonging to the genus Barbados, especially the scent of the cultivar group of the edible fruit, which refers to the fruit. The plant is mainly planted in East Asia such as the country, Taiwan, etc.; Philippines, Indonesia, country, and culture in Central and South Asia; India and other West Asia: Pakistan, Ecuador, South, etc. East Gua, Costa Rica West America, Guatemala, Colombia, Peru, etc. South America "曰: To non-specialized Africa, it also implements large-scale farming cultivation and cultivates throughout the year. In this case, the countries are 祛+立^t right to fight the world. Cultivated, the harvested scent "advanced in banana cultivation" contains the roots of the stems in the underground, high-stretched upper parts such as the stems are called pseudo-stems, in fact, the leaves overlap several layers, from the front of the pseudo-stem The large elliptical leaf (leaf body) is extended greatly, and the fruit axis (PedUneU) is produced from the front end of the pseudo stem. The first part is the whole box containing the finger and the utricle. After the harvest of the fragrance, the pseudo-stem (mother plant) is cut at 2m above and below the ground. 7 At this time, the new pseudo-stem (sub-strain) will be newly emerged from the root of the underground. Propagation in the same way, or the new pseudo-stem (sub-strain) can be propagated to reproduce the harvest of bananas. For the cultivation of fragrant coke, it is important to leave a few leaves (function leaves), because the last leaves are extracted (before flowering) ) It is necessary to harvest for 3 months, so it is necessary to leave the functional leaves to about 12 to 13 pieces during the flowering period. If the number of functional leaves is insufficient, the plants are cut off during the cultivation. 201208571 In the main diseases of banana cultivation, there are leaf spot and yellow yellow leaf spot disease (Yell Ow Sigatoka) and Black Sigatoka ° Yellow leaf spot is caused by Myc〇sphaerella mu si col a, black leaf spot is caused by Fischerella fiscus (Mycosphaerella fijiensis) The disease, especially black leaf spot, is a disease that occurs when the leaves of the banana turn black and hinder light synthesis and halve the amount of harvest when the temperature is high and humid. The incubation period of leaf spot is 3 weeks to 2 months later' The initial symptom of Luo Xiang's fragrance is that there are small spots on the back of the leaves of the ancient leaves from 4 to 5 pieces from the parietal lobe. Soon, the yellow streaks along the veins gradually enlarge the lesions and become dark brown. If it is aggravated, almost all the leaves will die and sag~ leaving only 1~2 of the central leaves. The prevention of these diseases begins with monitoring the leaves of the fragrant, and judges the position of the youngest leaves and the degree of infection. Whether or not to confirm the occurrence of leaf spot disease, organic sulfur-based fungicides such as Manc〇zeb and organic milk fungicides such as Chlorothalonil are used, and pirimethani is used. 1) Equal-density π-determination Morpholine, Tridemorph, Fenpropimorf and other morpholine fungicides, difenoconazole, Bitertanol, Propiconon, Epoxiconazole, etc. The three-salt bactericidal J 0 ratio. The key chain S 曰 (Pyraclostrobin), az S 旨 旨 (azoxystrobin) 荨 methoxy acrylate vinegar (str 〇bi 1 uri η) fungicide, these fungicides There are several kinds of spreads between the 10th and the 20th. In recent years, it is known that it does not exhibit bactericidal activity or weakness, but it is a disease-inducing agent (SAR agent) which is induced to resist disease. 201208571 Compound is useful as a disease control agent for various crops, and thiazide ( The general name .tiadinil) and isopridamide (general name: is〇tiann), the arsenic acid-S-methyl group (general name: acibenz〇iar-S-methyl), ciprofloxacin (also known as .carpropamid), culling heat (general name: probenazole), salicylic acid, dichloronicotinic acid, etc. are the aforementioned resistance inducing agents. Further, 2',4'-dimethoxy-4-cyclopropyl_ι, 2,3·嗟2, which is a compound similar to thiabicide. In the case of a known compound which is useful as a plant disease controlling agent (see, for example, Patent Document 1), it is considered to be a resistance inducing agent. Among these resistance-inducing agents, it is known that isotianil is effective in spreading the black leaf spot of bananas (see, for example, Patent Document 2). The compound represented by the general name or the chemical name is a known compound, which can be purchased from a commercially available product or produced by a method described in a publicly known document (see, for example, Non-Patent Document 1). [PRIOR ART DOCUMENT] [Patent Document 1] [Patent Document 1] Japanese Patent Publication No. 2 0 0 7 - 9 9 7 4 9 [Patent Document 2] International Publication 20 1 〇/〇3 7482 Booklet [Non-Patent Document] [Non-Patent Document 1] The Pesticide Manual 14th Edition (British Crop Production Council) [Summary of the Invention] [Problems to be Solved by the Invention] As described above, 'control of leaf spot disease is the most important issue for banana cultivation, but it is a conventional technique. Due to the implementation of most of the return of the drug treatment, its labor expansion, and more 201208571 = patience: the emergence of it, making prevention more difficult. Therefore, it is necessary to prevent and remove the trap: the leaf spot disease can effectively prevent the environmental load from being less, and it has the advantages of reducing the possibility of dispersing the tincture, and the labor-saving novel control method. * The inventors of the present invention have repeatedly tried their best to solve the above problems: by using the banana leaf spot control agent on the pseudo stem of the banana (the sheath and sheath are injected into the sheath and the like, then the regulation table b 士士中中> j found that this disease is effective to prevent and control: Secondly, the method of controlling the invention is finally completed. That is to say, the method for controlling the injection of the pseudo-stem into the liquid can occur outside the peak of the effective It can also prevent leaf spot disease that occurs in the new pseudo-stem (sub-strain) of Xindidi, which is better than the leaf spot control agent: = can achieve a significant long-term control effect, and can be the burden of the drug spreader, finally The present invention has been completed. That is, the present invention relates to at least the following inventions: (2) Plants of the color-cooking plants in which leaf spot disease occurs or are predicted to occur, and the effective amount of leaf spots is to prevent the t-part (Yale) The disease control agent is injected and treated, and: 'The leaf spot disease of the plant of the above-mentioned plant, the leaf spot disease prevention method of the spot disease. Previously (if, the spot disease control agent is a resistance inducer or a penetrating fungicide) The method of controlling leaf spot disease of fans (1). (3) Resistance inducement The agent is a reading of a A (2,,4,_: ^ A thiazide, isothiazide, compound phenyl-s::-4: cyclopropyl-1,2,3-thiadiazole -5·Carboxyguanidamine), alkanoic acid (2): leaf "propionamide, chlorpyrifos, salicylic acid, second gas end t) leaf spot disease prevention method. The aforementioned inducer is (four) base A method for controlling leaf spot disease according to any one of escarinamide or compound A. 201208571 (5) The permeable bactericide is a methoxy acrylate type (str〇bilurin) bactericide according to the above (2) (6) The methoxy acrylate bactericide is azoxystrobin, Metominostrobin, Picoxystrobin, pyraclostrobin (pyraclostrobin), deficiencies, vinegar, dioxin, vinegar or chloramphenicol (2) or (5) leaf spot disease prevention method. (7) immersion fungicide is an azole bactericide The method for controlling leaf spot disease according to the above (2). (8) The bactericidal fungicide is tebuconazole, spleen, propiconazole, cyproconazole, fluorova. Flusilazole Nitrile Saliva (myCi〇butanil), Flutriafol, triadimenol, hexaconazole, pentobacteria, all, olefin, Diniconazole, trimethoate, inoculum (ipC〇naz〇ie), Bromuconazole, Simeconazole, imibenconazole, pr〇thi〇c〇nazole or Imazalil The method for controlling leaf spot disease of the above (7). (9) The dad coke plant is the control method of leaf spot disease of the aforementioned (丨) of banana. (10) Leaf spot disease is a method for controlling leaf spot disease according to any one of the above (1) to (9) of the leaf spot disease of Mycosphaerella musicola. (11) Leaf spot disease is a method for controlling leaf spot disease according to any one of (1) to (9), which is caused by leaf spot disease of MyC〇Spharella fijiensis. 201208571 (1 2) Injecting and treating an effective amount of leaf spot control agent on the pseudo-stem portion (leaf sheath portion) of a banana mother plant in which leaf spot disease occurs or in order to prevent the occurrence of leaves of the banana seed strain of the aforementioned steamed mother plant A method for controlling leaf spot of banana seedlings characterized by spot disease. (1) The leaf spot disease control agent is a resistance inducer or a penetrating bactericide, and the method for controlling leaf spot disease of (12). (1 4) The resistance inducing agent is the aforementioned tiofenyl, isotianil, compound a, arsenic acid benzene-S-methyl, ciprofloxacin, chlorpyrifos, salicylic acid, dichlorinated acid (13) The method of controlling leaf spot disease. (1) The method for controlling leaf spot disease of the above (14) is a thioxyl group, isothiazide or compound a. (1 6)/The permeable fungicide is a method for controlling leaf spot disease of (14) before the sulfoxy acetonic acid bactericide. (1 7) 曱 oxy acrylate bactericides are azoxystrobin, phenoxystrobin S (Picoxystrobin), ° 〇 〇 趟 醋 py (pyraci〇 str〇bin), flurazepam, A method for controlling leaf spot disease of the above (17) of dipyridamole or anthracycline. (1 8 )> The fork-transmitting weighting agent is. The method for controlling leaf spot disease of the aforementioned (13) of the fungicide. (1 9 ) ° sitting sputum killing agent for tekki (tebuc ο na ζ ο 1 e), waiting for Klee, Propiconazole, gram, flusilazole, myclobutanil, defensive knife , Fickley, pentane, Diniconazole, trimethoate, inobutazole, bromide, silicopyrazole, imidazole, prothioconazole or imazalil (1 8 ) The control method of leaf spot disease. 201208571 [Effects of the Invention] The leaf spot of the present invention is effective in the prevention or control of the leaf spot disease. The method for controlling the leaf spot disease of the leaf spot is a treatment method which is superior to the treatment method, and the long-leaf bacteria are also excellent. Leaf spot disease prevention may reduce the amount of treatment 0 [Embodiment] [Form of the invention] The present invention may use a permeable bactericide, resistance: tiadinil), isothia glylate benzene-S-fluorenyl (. 醯Insectamine (general name: probenazole), phase p (dichloronicotinic or isotianilin is preferred. Name: azoxystrobin) Pyraclostrobin (Metominostrobin) disease control method is the Musa plant, especially for leaf spot disease The pseudo-stem portion of the plant body (the sheath sheathing agent injection treatment is characterized. The leaf treatment of the leaf spot disease control agent of the present invention has a short period of time indicating a significant control effect, a control effect for the medicament, and has a new underground The root (sub-strain) prevents the onset of leaf spot disease. This method is more likely to reduce the number of treatments of the drug, and 'is also a leaf spot disease prevention method that can reduce the environmental load control method. The agent is a resistance inducer or a immersion inducer; for example, thiabendazole (general name i-amine (general name: is 〇tiani 1), compound A, general name: acibenzolar-S-methyl), Carpropamid), culling heat (general name: salicylic acid, dichloro acid acid), especially thiabendamine, compound A penetrating fungicide can be used, for example, azoxystrobin (general, trifluoro Min (general name: trifloxystrobin), generic name: pyraclostrobin), phenoxystrobin, picoxystrobin (general name: picoxystrobin) 201208571, fluoride ^ pain vinegar (general name: fluoxastrobin), dioxin S ( General name: dimoxystrobin), sulphate (general name: orysastrobin), etc. 曱 丙烯酸 丙烯酸 丙烯酸 1 str str str str str str str ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( ( Name: epoxiconazole), Waiting for Klee (general name: difenoconazole) 'Puckley (general name: propiconazole), gram seat (general name: cyproconazole), fluorsparite mouth (general name: flusilazole), extant seat (general name) :metcon Azide, nitrile bacillus (general name: myclobutanil), quaternary celsius (general name: tetraconazole), fluoroquinone 0 sitting (general name: fliuquinconazole) 'protective fen (general name: flractaol), tri-sodium alcohol (general name) :triadimenol), Fickley (general name: hexaconazole), penicillium saliva (general name: penconazole), Bidanon (general name: bitertanol), sterilized cockroach (general name: triticonazole), cyanobenzene ° (general name: Fenbuconazole), dilute sterol (general name: diniconazole), trimethoate (general name: triadimefon), inoculum 0 sitting (general name: ipconazole), indigenous (general name: bromuconazole), wafu ° sitting (general name) :simeconazole), imidazole (general name: imibenc ο na ζ ο 1 e), prothiotoxin. Sitting (general name: prothioconazole), poker pull (general name: prochloraz), anti-mite sitting (general name: imaza 1 i 1), π 恶咪 ° sitting (ο X ρ 〇c ο na ζ ο 1 e ), etc. A salivary bactericide, but the leaf spot control agent which can be used in the present invention is not limited to these active ingredients. These agents are commercially available and are readily available from the market. Further, Compound A can be produced by the method described in Patent Document 1. The method for controlling leaf spot disease of the present invention can be carried out by injecting a commercially available liquid drug containing the above-mentioned compound as an active ingredient into a leaf portion of a plant such as banana, by injection or the like into an injection device such as an injection of -10-201208571. Further, the compound A can be dissolved in an appropriate inert carrier and a surfactant, if necessary, together with an adjuvant, in an appropriate ratio, to prepare a suitable dosage form, such as a suspension, a milk suspension, It is used in the preparation of emulsions, liquids, etc. The amount of the active ingredient to be added to the 00 parts by mass of the leaf spot disease can be suitably selected from the range of 0.1 part by mass to 50 parts by mass, preferably from 1 part by mass to 50 parts by mass. The inert carrier used in the method for controlling the present invention may be a liquid or solid inert carrier, and the method of injecting into the sheath portion is preferably a liquid carrier, which can be used as a liquid carrier material, and can be selected from the group consisting of a solvent and a solvent. Although it does not have solvent ability, it helps the adjuvant to disperse the active ingredient compound, and thus the liquid carrier can be, for example, water, alcohol (for example, decyl alcohol, ethanol, isopropanol, butanol, ethylene glycol, etc.), ketone. Classes (such as acetone, acetophenone, methyl butanone, diisobutyl ketone, cyclohexanone, etc.), ethers (such as ether, bisaki, fibrin, dipropylene, tetrahydro sulphate, etc.) , aliphatic hydrocarbons (such as kerosene, mineral oil, etc.), aromatic hydrocarbons (such as benzene, toluene, diphenyl, solvent oil, alkyl naphthalene, etc.), _ hydrocarbons (such as di-ethane, gas Imitation, four gasified carbon, etc.), esters (such as ethyl acetate, diisopropyl phthalate, dibutyl phthalate, dioctyl phthalate, etc.), guanamines (such as dimethyl decylamine, Diethylformamide, dimethylacetamide, etc.), nitriles (eg acetonitrile, etc.), diterpenes These may be used singly or in the form of a mixture of two or more. Other adjuvants may be exemplified by the following representative auxiliaries, which are used according to the purpose, and may be used alone or in some cases with 2 -11 - 201208571 or more adjuvants'. Do not use adjuvants at all. For the purpose of emulsifying, dispersing, solubilizing, and/or wetting the active ingredient compound, a surfactant may be used, and examples thereof include a polyoxyethylene alkyl bond, a polyoxyethylene alkyl aryl ether, and a polyoxyethylene higher fatty acid ester. , polyoxyethylene resin acid ester, polyoxyethylene sorbitan monododecanoate, polyoxyethylene sorbitan monooleic acid vinegar, aryl aryl acid salt, caic acid condensate, lignosulfonate, a surfactant such as a higher alcohol sulfate. As the debonding agent for the suspension product, for example, an auxiliary agent such as a naphthalenesulfonic acid condensate or a condensed phosphate can be used. As the antifoaming agent, an auxiliary agent such as polysiloxane oil can be used. Examples of plants in which the control method of the present invention can prevent leaf spot disease can be exemplified by Gros Michel, Cavendish, Dwarf Cavendish, Dwarf Chinese. , Enano, Caturra, Giant Cavendish, Gran Enano, Grande Naine, Williams Hybrid, Valery ), Robust, P〇y〇, Lacatan, Monte cristo, Bout rond, etc., but not limited to These Musa plants. It is particularly preferable to generally cultivate all of the bananas including the giant Jiayuntai, the cave Jiayuntai, the Jiayuntai, the blue sky, the red banana, the apple banana, the banana, and the island banana. The Musa plant which is the object of the control method of the present invention also includes a herbicide tolerance gene, an insecticidal pest resistance gene, an anti-pathogenic substance-generating gene, an oil grain component modification or an amino acid content-enhancing shape, and the like. A useful form of genetically modified gene shuffle (GMO) homologous plants. -12- 201208571 In order to prevent leaf spot of Musa plants, the control agent may be used as such, or diluted appropriately with water, or in the form of a suspension to prevent leaf spot disease from occurring or predicting the occurrence of leaves. The scent of spot disease f, the plant body ί Ϊ Ϊ ' can be injected using an injector such as a syringe. The fruit of the injection site and the medicine of the right-handed banana can be injected into the growth zone of the residual pseudo-stem portion at a distance of about 3 cm~lm from the surface of the ground of the remaining pseudo-stem portion after harvesting. It can prevent the leaf spot disease that occurs in the growing fragrant coke. If it is harvested, it can also prevent the occurrence of the leaf stalk treatment. It can not only prevent the leaf spot disease of the mother plant itself, but also prevent the leaf spot disease of the plant. . The banana leaf spot control agent of the present invention can be formulated into a common dosage form, such as an emulsion, a water and a granule water, a granule water and a granule, and a floatable dosage form. The treatment amount is based on the ratio of the active ingredients = condition, formulation form, treatment What is the time, processing method, treatment location, etc.? In the case of the emulsion which is usually used in large-scale agricultural cultivation in general, the number of cultivars per banana of A is cultivated from about 1,500 to 2,000, and the medicinal agent is distributed in a dose of 0.4 per hectare. Bu 31 spread 'so the equivalent of the amount of each plant. According to the method of the present invention, the drug solution diluted to a predetermined dose can be injected into the pseudo stem portion by, for example, a syringe or the like according to the dose of 0.2 ml to 2 nU per plant. The amount converted to the active ingredient amount is in the range of 60 mg to 40 mg per plant of the plant of the fragrant f', but the amount in the present invention is not limited thereto. According to the control method of the present invention, the amount of the medicament can be reduced, which is different from the situation of being dispersed by the user, and the user is not exposed to the liquid medicine (the situation of "spreading" can safely prevent leaf spot disease. -13- 201208571 In the leaf spot prevention method of the present invention, the agricultural and horticultural fungicides or the agricultural and horticultural insecticides other than the above-mentioned fungicides can be appropriately mixed and used, and thus the agricultural and horticultural fungicides or the agricultural and horticultural insecticides are represented. The compound is exemplified as follows, but the above-mentioned agricultural and horticultural fungicides or agricultural and horticultural insecticides are not limited thereto. The agricultural and horticultural fungicides may be, for example, isoprothiolane, tricyclic, tricyclazole, and sputum. Azoxystrobin, 〇比. Pyroclostrobin, propiconazole, coPPer-8-quinolinolate, Metominostrobin, carpropamid ), fthalide, kasugamycin, pyroquilon, probenazole, acibenzolar-S-methyl, fudo Ning (flutolanil), extinction Mepronil, furametpyr, pencycuron, thifluzamide, validamycin, diclomezine, ferimzone, diterpene Salt (guazatine), nitrile bacteria, sitting (myclobutanil), hexaconazole, difenoconazole, bitertanol, epoxiconazole, triadimefon (triadimefon), poker Prochloraz, fenpropimorph, tridemorph, metalaxyl, copper oxychloride, basic copper sulfate, manganese zinc Mancozeb, propineb, Ziram, thiuranl, cymoxanil, chl〇rothalonil, Kelly-14 201208571 (difenoconazole), captan, polyoxiii, iprodione, procymidone, benomyl, thiophanate-methyl, Carbendazim, Forsyth Fosetyl-aluminium), hymexazol, oxolinic acid, fludiox.onil, mepanipyrim, cyprodinil, pyramimethanil ), a mixture of agricultural and horticultural fungicides such as tolclofos-methyl and fenoxanil. The agricultural and horticultural insecticide may be, for example, a nocturnal (general name: benfuracarb), a butyl plus abundance (general name: carbosulfan), a PHC (general name: propoxur), a ruthenium (general name: furathiocarb), Nai (general name: methodylyl), NAC (general name: carbaryl), invincible (general name: bendiocarb), cotton ring (general name: alanycarb), Yidashun (general name imidacloprid), Nit . Bi Lan (general name: nitenpyram), sub-killer (general name: acetamiprid), Sai Suan (general name thiamethoxam), gram (general name: clothianidin), Datnam (general name: dinotefuran), nursing Pine (general name: prop.aphos), B-mixed (general name: disulfoton), Diyan (general name: diazinon), Sanqisong (general name: trichlorfon), Fenicide (general name: fenthion), Eurasian pine (general name: acephate), plusfusone (general name: isoxathion), abifensone (general name: isofenphos), methyl poison monument (general name: chlorpyrifos-methyl), killing Wei (general name: Chlorfenvinphos), Dasongsong (general name: dimethoate), Dafusong (general name: fonofos), bifensone (general name: pyridafenthion), sub-soul (general name: monocrotophos) ' Pei-15- 201208571 Dan (General Name · cartap), thiosulfan (general name: thiocyclam), speed-free (general name: bensultap) 'Fenilerin (general name · cycloprothrin), efenine (general name: etofenprox), 矽保芬 ( General name: silafluofen), Flumethrin (general name · tefiuthrin), buffing net (general name: buprofezin), flufenaphan (general name: flufenoxuron), cyanobacteria (general name: cyromazine), lufenlong (general name: lufenuron) , Defeno (general name: tebufenozide), aerobic worm (general name: methoxyfenozide), cofino (general name: chromafenozide), euthion (general name: oxamyl), baikesong (general name: pyraclofos) , Forsythia (general name: fosthiazate), Fenpuff (general name: fenpyroximate), Fenpney (general name: fipronil), Anzai Fan (general name: endosulfan), polyethylbenzene (general name: metaldehyde) » Because of the killing >, Ding (general name: emamectine-benzoate) 'Sinuo kill (general name: spinosad), Kefan pie (general name: chlorfenapyr), Indak (general name: indoxacarb) and other mixed use. [Examples] Representative prescription examples and test examples of the present invention are exemplified below, but the present invention is not limited thereto. In the case of the prescription or the test, the part is a part by weight. Formulation Example 1. Active ingredient of leaf spot control agent of the present invention 50 parts of diphenylbenzene 40 parts Mixture of polyoxyethylene nonylphenyl ether and calcium alkylbenzenesulfonate 10 parts The above is uniformly mixed and dissolved into an emulsion . -16 - 201208571 Formulation Example 2. 30 parts 5 parts 5 parts 2 parts 58 parts Active ingredient of leaf spot control agent of the present invention Sorpol3105 (manufactured by Toho Chemical Industry Co., Ltd.) propylene glycol
Rodoporu(Rh0ne_P〇ulenc 公司製） 水 將以上均勻混合，分散於水作成可浮劑 處方例3. 15份 5份 5份 2份 73份 異噻菌胺 Sorpol3 1 05 丙二醇 Rodoporu 水 分散於水作成可浮劑 將以上均勾混合 試驗例1. 依各種藥劑之注入處理之香奪葉斑病防除試驗。 於葉斑病之發生始期之香蕉，將噻醯菌胺及化合物 A為處方例2、異噻菌胺為處方例3之各別可浮劑、吡唑 醚菌酯為市售品之25%乳^劑，而錳鋅乃浦為市售品之 80%水和劑以水稀釋成所定藥量，於母偽莖部以改良之 注射器於地上部5〇cm左右處’依每株注入稀釋液 藥劑處理後第16日起每約2星期算出健全之機能 葉。1區2 0連制。 再者，母偽莖處理區之機能葉之片數乃示由同根部 長出之子偽莖之機能葉之片數。 結果如第1表戶斤禾° -17- 201208571 [第1表] 藥劑 藥量 (有效成分g/ha) 機能葉數/株 處理 1曰前 處理 16曰後 處理 30曰後 處理 46日後 噻醯菌胺 900 7.90 9.33 10.46 11.46 300 7.54 9.24 10.19 11.73 異噻菌胺 150 7.06 8.17 9.49 10.80 50 6.92 9.05 10.04 11.51 化合物A 180 6.34 8.25 9.25 10.77 60 6.53 8.38 9.59 10.92 °比。坐醚函酉旨 100 6.45 8.37 9.62 10.28 33.3 6.39 8.48 9.46 10.11 錳鋅乃浦 1600 5.96 7.95 8.36 9.19 533.3 6.44 8.38 8.58 9.65 無處理 7.55 7.80 8.50 9.00 由本試驗結果得知，屬於抵抗性誘導劑之'•塞酿菌胺 、異噻菌胺、化合物 A、及屬於浸透性殺菌劑之吡唑醚 菌酯之向母株之偽莖部之注入處理區，比無處理長期抑 制香蕉葉斑病之發病及進展，而保有多數機能葉，其防 除效果顯著，長期維持對香蕉之生育無障碍之狀態。另 一方面，屬於接觸型殺菌劑之錳鋅乃浦乃於同樣之處理 方法，機能葉數為與無處理同程度，無顯示明顯之防除 效果。 亦即，依抵抗性誘導劑或浸透性殺菌劑之本發明之 防除方法，顯然可達成抑制植物體全體由於香蕉葉斑病 之被害之顯著防除效果。又，本發明之防除方法，顯然 也可達成抑制對母株之偽莖部之處理於次世代之植物體 發生葉斑病之特別效果。 -18- 201208571 試驗例2。塞醯菌胺對香蕉生長期發生之葉斑病防除 試驗 葉斑病之發生始期之香蕉’將處方例2之製劑處方 之藥劑（有效成分：噻醯菌胺）以水稀釋5倍之藥液2ml 於子株或母株之偽莖部以注射器注入地上部1 m左右之 南處’藥劑處理後第1丨日起算出每株之機能葉（健全地 機能之葉）之總片數’其後每1星期同樣地算出9星期（j 區4連制再者，母偽莖處理區之機能葉之片數乃示由 同根部長出之子偽莖之機能葉之片數。 結果如第2表所示。Rodoporu (Rh0ne_P〇ulenc) Water is uniformly mixed above and dispersed in water to form a floatable agent. 3.15 parts 5 parts 5 parts 2 parts 73 parts Isoprofen Sorpol3 1 05 Propylene diol Rodoporu Water is dispersed in water. The granules are mixed with the above test examples. 1. The fragrant leaf spot control test according to the injection of various medicinal agents. For bananas at the beginning of leaf spot disease, thiabendazole and compound A are prescription 2, isothiazide is the respective floatable agent of prescription 3, and pyraclostrobin is 25% of the commercial product. The milk agent is used, and the 80% water and the agent of the commercial product of the manganese and zinc is diluted with water to a predetermined dose, and the mother's pseudo stem is diluted with a modified syringe at about 5 cm above the ground. The functional leaf is calculated every about 2 weeks from the 16th day after the liquid medicine treatment. Zone 1 2 0 system. Furthermore, the number of functional leaves of the mother pseudo-stalk treatment area is the number of functional leaves of the pseudo-stems which are grown by the same root. The results are as shown in the first table, jinhe ° -17-201208571 [Table 1] Pharmacy dose (active ingredient g/ha) Functional leaf number/plant treatment 1 曰 pretreatment 16 曰 post treatment 30 曰 post treatment 46 days later thiazide Amidonin 900 7.90 9.33 10.46 11.46 300 7.54 9.24 10.19 11.73 Isotianil 150 7.06 8.17 9.49 10.80 50 6.92 9.05 10.04 11.51 Compound A 180 6.34 8.25 9.25 10.77 60 6.53 8.38 9.59 10.92 ° ratio. Ether ether purpose 100 6.45 8.37 9.62 10.28 33.3 6.39 8.48 9.46 10.11 Manganese zinc is 1600 5.96 7.95 8.36 9.19 533.3 6.44 8.38 8.58 9.65 No treatment 7.55 7.80 8.50 9.00 Known from the results of this test, it is a resistance inducer Injecting treatment zone of chimeric amine, isothiazide, compound A, and pyraclostrobin which is a penetrating fungicide to the pseudo-stem of the mother plant, inhibiting the onset and progression of banana leaf spot disease over a long period of time without treatment While retaining most functional leaves, its control effect is remarkable, and it maintains the state of barrier-free access to bananas for a long time. On the other hand, the manganese-zinc-pyrogen which is a contact type bactericide is the same treatment method, and the number of functional leaves is the same as that of no treatment, and no obvious control effect is shown. That is, it is apparent that the control method of the present invention according to the resistance inducing agent or the penetrating bactericidal agent can achieve a significant control effect of suppressing the damage of the whole plant body due to banana leaf spot disease. Further, in the method for controlling the present invention, it is apparent that the special effect of suppressing the treatment of the pseudo-stem portion of the mother plant on the leaf spot of the next generation plant body can be achieved. -18- 201208571 Test Example 2. Leaf spot control test for the growth period of banana during the growth period of bananas. Banana of the beginning of leaf spot disease. The prescription of the prescription of prescription 2 (active ingredient: thiabicide) is diluted 5 times with water. 2ml The number of the functional leaves of each plant (the leaf of the sound functional function) is calculated from the first day after the treatment of the drug. The number of functional leaves of the female pseudo-stalk processing area is the number of functional leaves of the pseudo-stem of the son of the same root. The result is as shown in the second table. Show.
I-— ---- 1。训| /·/：) ί 試驗規模：每1處理4植物體 處理器具：l〇ml之注射器 處理量：2._/株(於散布處理相當於3 2公升如之量） 由本試驗結果得知，屬於噻醯菌胺注入處理區，丨 ^ f理即使為對母株之偽莖部之注入處理、對子株之4 二。卩之/主入處理，可長期抑制香蕉葉斑病之發病及進乂 ’而維持機能笼彳？ 葉 片以上，其防除效果顯著，且長期ί 焦之生育無障礙之狀態。。亦即，可明白本發日 ^防除方法對於植物體全體由葉斑病被害可達成抑^多 ^ ^可明白本發明之防除方法也對母株之偽莖部： :。於:人世卩之植•體抑㈣斑病之發生可達成特別彡 201208571 試驗例3 , 將與試驗例2相同之藥劑以同樣之方法處理。作為 處理效果之指標，算出由上部之最新葉計數至最幼感染 葉之位置之健全葉之每1株之片數（1區4連制）。再者， 母偽莖處理區之健全葉數乃示由同根部長出之子偽莖之 健全葉數。 結果如第3表所示。 [第3表] 處理區 於各處理後曰數之上部新葉計數之 至最幼感染葉位置之健全葉之片數/株 0 11 18 25 32 39 46 53 60 67 74 子偽莖處理區 8.00 10.75 11.25 10.50 10.75 12.00 12.50 12.50 12.00 11.00 10.75 母偽莖處理區 8.25 12.00 13.00 12.25 12.00 11.55 12.00 13.25 13.00 13.00 11.50 無處理區 1 8.00 6.75 7.25 9.00 8.00 9.00 9.00 10.50 10.25 10.25 10.25 葉斑病之感染之潛伏期間為3星期至2個月左右 於無處理區，香蕉之生育期所必要之機能葉因感染而片 數減少，無法充分生育。反觀依本發明有效成分之一之 嗔酿菌胺之防除方法實施區，由於葉斑病防除劑之注入 處理：可抑制葉斑病感染之擴大，其結果健全葉數增加 香’、’、充刀生月、准持向品質。又其防除效果也維持藥 之例行防除相較，可圖謀散布 對散布者及環境之負荷。I-- ---- 1. Training | /·/:) ί Test scale: 4 treatments per plant treatment: l〇ml syringe treatment capacity: 2._/ strain (in the amount of 3 2 liters in the dispersion treatment) It is known that it belongs to the thiabicide injection treatment zone, and even if it is the injection treatment of the pseudo-stem of the mother plant, the pair of sub-strains.卩之/主入处理, can inhibit the onset and progression of banana leaf spot disease for a long time ‘ while maintaining functional cages? Above the leaf, the control effect is remarkable, and the long-term ί focus is in a state of barrier-free birth. . That is, it can be understood that the prevention method of the present invention can achieve a total inhibition of leaf spot disease in the whole plant body. It can be understood that the control method of the present invention also applies to the pseudo stem portion of the mother plant:于: The human body 卩 • 体 体 体 四 四 四 四 四 四 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 085 As the index of the treatment effect, the number of pieces of each of the healthy leaves from the latest leaf count of the upper part to the position of the youngest infected leaf was calculated (1 zone 4 joint system). Furthermore, the number of healthy leaves of the mother pseudo-stem treatment area is the number of healthy leaves of the pseudo-stem of the son of the same root. The results are shown in Table 3. [Table 3] Number of healthy leaves in the treatment area at the upper part of the number of new leaves counted to the position of the youngest infected leaves / strain 0 11 18 25 32 39 46 53 60 67 74 Sub-pseudo-stalk treatment area 8.00 10.75 11.25 10.50 10.75 12.00 12.50 12.50 12.00 11.00 10.75 Female pseudo-stalk treatment area 8.25 12.00 13.00 12.25 12.00 11.55 12.00 13.25 13.00 13.00 11.50 No treatment area 1 8.00 6.75 7.25 9.00 8.00 9.00 9.00 10.50 10.25 10.25 10.25 The incubation period for leaf spot infection is In the no-treatment area of about 3 weeks to 2 months, the functional leaves necessary for the growth period of bananas are reduced due to infection and cannot be fully fertilized. In contrast, according to one of the active ingredients of the present invention, the method for controlling the method of controlling the geranium is due to the injection treatment of the leaf spot control agent: the expansion of the leaf spot infection can be inhibited, and the result is that the number of healthy leaves is increased by ', ', and The knife is born and the quality is maintained. In addition, the control effect also maintains the routine control of the drug, and can be used to spread the load on the spreader and the environment.
更進一步，與習知 數、藥量之減低、減低 【圖式簡單說明】 無。 【主要元件符號說明】 〇 *»»、 -20-Further, the number of the known and the amount of the drug is reduced, and the amount of the drug is reduced. [Main component symbol description] 〇 *»», -20-