TR200101179T2 - Nükleik asit oligomer hibritlerinin elektrokimyasal olarak tayin metodu - Google Patents
Nükleik asit oligomer hibritlerinin elektrokimyasal olarak tayin metoduInfo
- Publication number
- TR200101179T2 TR200101179T2 TR2001/01179T TR200101179T TR200101179T2 TR 200101179 T2 TR200101179 T2 TR 200101179T2 TR 2001/01179 T TR2001/01179 T TR 2001/01179T TR 200101179 T TR200101179 T TR 200101179T TR 200101179 T2 TR200101179 T2 TR 200101179T2
- Authority
- TR
- Turkey
- Prior art keywords
- hybridization
- support surface
- nucleic acid
- acid oligomer
- determination method
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
- C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
- C12Q1/6816—Hybridisation assays characterised by the detection means
- C12Q1/6825—Nucleic acid detection involving sensors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
Abstract
Bulus, dizi-spesifik nükleik asit oligomeri hibridizasyon olaylarinin elektrokimyasal tayini için kullanilan bir metodla ilgilidir. Buna göre, bir uçta destek yüzeye kovalent baglanmis, diger serbest uçta redoks çiftine kovalent baglanmis tek DNA/RNA/PNA oligomer iplikleri, hibridizasyon matrisi (prob) olarak kullanilir. Incelenecek olan oligonucleotide solüsyonu (hedef) ile muamele sonucunda, iletken destek yüzey ve tek-iplikli oligonucleotide ile köprülenmis redoks çifti arasindaki elektrik iletisimi (baslangiçta mevcut olmayan veya çok zayif olan ) modifiye edilir. Hibridizasyon durumunda, destek yüzey ile (hibritlenmis çift-iplikli oligonucleotide ile simdi köprülenmis olan) redoks çifti arasindaki elektrik iletisimi artirilir. Bu, çevrimsel voltametri, amperometri veya iletkenlik ölçümü gibi elektrokimyasal metodlarla hibridizasyon olayinin tayinine izin verir.
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
DE19853957 | 1998-11-23 | ||
DE19921940A DE19921940C2 (de) | 1998-11-23 | 1999-04-29 | Verfahren zur elektrochemischen Detektion von Nukleinsäureoligomerhybriden |
Publications (1)
Publication Number | Publication Date |
---|---|
TR200101179T2 true TR200101179T2 (tr) | 2001-11-21 |
Family
ID=26050314
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
TR2001/01179T TR200101179T2 (tr) | 1998-11-23 | 1999-11-19 | Nükleik asit oligomer hibritlerinin elektrokimyasal olarak tayin metodu |
Country Status (13)
Country | Link |
---|---|
US (1) | US7056664B1 (tr) |
EP (1) | EP1133514A1 (tr) |
JP (1) | JP2002532386A (tr) |
CN (1) | CN1324365A (tr) |
AU (1) | AU751220B2 (tr) |
BR (1) | BR9915526A (tr) |
CA (1) | CA2345157A1 (tr) |
DE (1) | DE19964220C2 (tr) |
IL (1) | IL142758A0 (tr) |
PL (1) | PL348144A1 (tr) |
RU (1) | RU2213095C2 (tr) |
TR (1) | TR200101179T2 (tr) |
WO (1) | WO2000031101A1 (tr) |
Families Citing this family (27)
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US6649350B2 (en) * | 1997-04-09 | 2003-11-18 | California Institute Of Technology | Electrochemical sensor using intercalative, redox-active moieties |
DE59915204D1 (de) | 1998-08-28 | 2010-10-28 | Febit Holding Gmbh | Verfahren zur herstellung von biochemischen reaktionsträgern |
DE19964220C2 (de) | 1998-11-23 | 2003-07-03 | Friz Biochem Gmbh | Verfahren zur Herstellung einer modifizierten leitfähigen Oberfläche |
AU758063B2 (en) * | 1999-01-18 | 2003-03-13 | Friz Biochem Gesellschaft Fur Bioanalytik Mbh | Method for electrochemically detecting nucleic acid-oligomer hybridisation events |
DE19945398A1 (de) * | 1999-09-22 | 2001-04-05 | Friz Biochem Gmbh | Elektrochemische Detektion von sequenzspezifischen Nukleinsäure-Oligomer-Hybridisierungsereignissen |
DE10049527A1 (de) * | 2000-10-06 | 2001-09-06 | Friz Biochem Gmbh | Thermostabile,photoinduzierbar redoxaktive Einheit zur elektrochemischen Detektion von Nukleinsäure-Oligomer-Hybridisierungsereignissen |
DE10051396A1 (de) | 2000-10-17 | 2002-04-18 | Febit Ferrarius Biotech Gmbh | Verfahren und Vorrichtung zur integrierten Synthese und Analytbestimmung an einem Träger |
AU2002345746A1 (en) * | 2001-06-21 | 2003-01-08 | The Regents Of The University Of California | Electrochemical detection of mismatch nucleic acids |
KR100467778B1 (ko) * | 2001-07-25 | 2005-01-24 | 주식회사 포스코 | 핵산 혼성화 반응의 검출방법 |
DE10141691A1 (de) * | 2001-08-25 | 2003-03-13 | Friz Biochem Gmbh | Verdrängungsassay zur Detektion von Ligat-Ligand-Assoziationsereignissen |
WO2003018834A2 (de) | 2001-08-25 | 2003-03-06 | Friz Biochem Gmbh | Verdrängungsassay zur detektion von nukleinsäureoligomer-hybridisierungsereignissen |
DE10155054C2 (de) * | 2001-11-09 | 2003-10-23 | Friz Biochem Gmbh | Molekulares elektronisches Bauelement zum Aufbau nanoelektronischer Schaltungen, molekulare elektronische Baugruppe, elektronische Schaltung und Herstellungsverfahren |
DE10155031A1 (de) * | 2001-11-09 | 2003-05-28 | Friz Biochem Gmbh | Fluoreszenz-Quenchen zur Detektion von Ligat-Ligand Assoziationsereignissen in elektrischen Feldern |
US7413859B2 (en) * | 2001-11-14 | 2008-08-19 | Siemens Aktiengesellschaft | Method and biosensors for detecting macromolecular biopolymers |
WO2004011643A1 (ja) * | 2002-07-26 | 2004-02-05 | Kabushiki Kaisha Toshiba | 核酸プローブ固定化基体およびそれを用いた標的核酸の存在を検出する方法 |
WO2004023128A1 (en) * | 2002-09-05 | 2004-03-18 | Unisearch Limited | Detection of target nucleic acid molecules by alteration of reaction of a redox species following hybridization with immoblized capture nucleic acid molecules |
MXPA05011909A (es) | 2003-05-02 | 2006-09-04 | Ct Biolog Medycznej I Mikrobio | Derivado de nucleosido, oligonucleotido modificado, metodo para su sintesis y aplicaciones de los mismos. |
JP2005292007A (ja) * | 2004-04-01 | 2005-10-20 | Seiko Epson Corp | 核酸固定化方法およびそれを用いるバイオセンサの製造方法 |
US20060199241A1 (en) * | 2005-03-02 | 2006-09-07 | Hyoungsik Yim | Electrochemically and optically monitoring cleaving enzyme activity |
US8298391B2 (en) * | 2007-07-11 | 2012-10-30 | Silveri Michael A | Amperometric sensor |
FR2932191B1 (fr) * | 2008-06-05 | 2010-12-03 | Univ Paris Diderot Paris 7 | Methode d'identification electrochimique de sequences cibles de nucleotides. |
US8887556B2 (en) | 2011-02-15 | 2014-11-18 | Michael A. Silveri | Amperometric sensor system |
CN102408745B (zh) * | 2011-11-25 | 2014-06-18 | 北京科技大学 | 一类以四苯基卟啉为核心的不对称染料分子及其制备方法 |
CN103115954B (zh) * | 2013-01-31 | 2014-07-09 | 中国人民共和国泰安出入境检验检疫局 | 采用蒽醌-2-磺酸钠传感器快速测定食品中三聚氰胺的方法 |
JP2019503032A (ja) | 2015-11-17 | 2019-01-31 | ネグゼオン・リミテッドNexeon Ltd | 官能化された電気化学的活性材料および官能化の方法 |
GB2544494B (en) * | 2015-11-17 | 2018-10-24 | Nexeon Ltd | Functionalised electrochemically active material and method of functionalisation |
GB2544495B (en) | 2015-11-17 | 2018-12-05 | Nexeon Ltd | Surface modified electrochemically active material |
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US3662745A (en) * | 1969-06-30 | 1972-05-16 | Hoffmann La Roche | Metal-metal salt electrode and method for making the same |
US5102798A (en) | 1988-09-08 | 1992-04-07 | Allage Associates | Surface functionalized Langmuir-Blodgett films for immobilization of active moieties |
US5457183A (en) | 1989-03-06 | 1995-10-10 | Board Of Regents, The University Of Texas System | Hydroxylated texaphyrins |
WO1993021530A1 (de) | 1992-04-10 | 1993-10-28 | Messerschmitt-Bölkow-Blohm Gmbh | Verfahren und vorrichtung zur empfindlichkeits- und selektivitätssteigerung bei immuno-assays, molekül-rezeptor-, dna-komplementär-dna- und gast-wirtsmolekül-wechselwirkungs-assays |
US5312527A (en) | 1992-10-06 | 1994-05-17 | Concordia University | Voltammetric sequence-selective sensor for target polynucleotide sequences |
US5824473A (en) | 1993-12-10 | 1998-10-20 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5591578A (en) * | 1993-12-10 | 1997-01-07 | California Institute Of Technology | Nucleic acid mediated electron transfer |
US5622821A (en) | 1994-06-29 | 1997-04-22 | The Regents Of The University Of California | Luminescent lanthanide chelates and methods of use |
EP0824684B1 (en) | 1995-05-12 | 2007-11-07 | Novartis AG | Method for the parallel detection of a plurality of analytes using evanescently excited luminescence |
US5968745A (en) * | 1995-06-27 | 1999-10-19 | The University Of North Carolina At Chapel Hill | Polymer-electrodes for detecting nucleic acid hybridization and method of use thereof |
DE69734520D1 (de) | 1996-06-07 | 2005-12-08 | Amersham Biosciences Sv Corp | Nukleinsäurevermittelter elektronentransfer |
DE19639169A1 (de) | 1996-09-24 | 1998-04-02 | Boehringer Mannheim Gmbh | Redoxaktive Verbindungen und deren Anwendung |
CA2270633A1 (en) | 1996-11-05 | 1998-05-14 | Clinical Micro Sensors | Electrodes linked via conductive oligomers to nucleic acids |
US6306584B1 (en) | 1997-01-21 | 2001-10-23 | President And Fellows Of Harvard College | Electronic-property probing of biological molecules at surfaces |
US6221586B1 (en) * | 1997-04-09 | 2001-04-24 | California Institute Of Technology | Electrochemical sensor using intercalative, redox-active moieties |
US6060327A (en) * | 1997-05-14 | 2000-05-09 | Keensense, Inc. | Molecular wire injection sensors |
IL121312A (en) | 1997-07-14 | 2001-09-13 | Technion Res & Dev Foundation | Microelectronic components, their manufacture and electronic networks containing them |
DE19964220C2 (de) | 1998-11-23 | 2003-07-03 | Friz Biochem Gmbh | Verfahren zur Herstellung einer modifizierten leitfähigen Oberfläche |
-
1999
- 1999-04-29 DE DE19964220A patent/DE19964220C2/de not_active Expired - Fee Related
- 1999-11-19 CN CN99812448A patent/CN1324365A/zh active Pending
- 1999-11-19 AU AU13836/00A patent/AU751220B2/en not_active Ceased
- 1999-11-19 RU RU2001114192/04A patent/RU2213095C2/ru not_active IP Right Cessation
- 1999-11-19 CA CA002345157A patent/CA2345157A1/en not_active Abandoned
- 1999-11-19 JP JP2000583928A patent/JP2002532386A/ja active Pending
- 1999-11-19 US US09/856,543 patent/US7056664B1/en not_active Expired - Fee Related
- 1999-11-19 IL IL14275899A patent/IL142758A0/xx unknown
- 1999-11-19 WO PCT/EP1999/008888 patent/WO2000031101A1/de not_active Application Discontinuation
- 1999-11-19 BR BR9915526-5A patent/BR9915526A/pt not_active IP Right Cessation
- 1999-11-19 TR TR2001/01179T patent/TR200101179T2/tr unknown
- 1999-11-19 PL PL99348144A patent/PL348144A1/xx not_active Application Discontinuation
- 1999-11-19 EP EP99972637A patent/EP1133514A1/de not_active Withdrawn
Also Published As
Publication number | Publication date |
---|---|
US7056664B1 (en) | 2006-06-06 |
EP1133514A1 (de) | 2001-09-19 |
CN1324365A (zh) | 2001-11-28 |
IL142758A0 (en) | 2002-03-10 |
WO2000031101A1 (de) | 2000-06-02 |
BR9915526A (pt) | 2001-11-13 |
PL348144A1 (en) | 2002-05-06 |
RU2213095C2 (ru) | 2003-09-27 |
AU751220B2 (en) | 2002-08-08 |
AU1383600A (en) | 2000-06-13 |
CA2345157A1 (en) | 2000-06-02 |
DE19964220C2 (de) | 2003-07-03 |
JP2002532386A (ja) | 2002-10-02 |
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