SU668939A1 - Method of separating aqueous culture medium - Google Patents

Method of separating aqueous culture medium

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Publication number
SU668939A1
SU668939A1 SU772504952A SU2504952A SU668939A1 SU 668939 A1 SU668939 A1 SU 668939A1 SU 772504952 A SU772504952 A SU 772504952A SU 2504952 A SU2504952 A SU 2504952A SU 668939 A1 SU668939 A1 SU 668939A1
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SU
USSR - Soviet Union
Prior art keywords
suspension
decanter
yeast
contact surfaces
aqueous medium
Prior art date
Application number
SU772504952A
Other languages
Russian (ru)
Inventor
Любберт Густав-Адольф
Хайденрайх Гюнтер
Бинек Норберт
Клингер Райнгард
Райтер Урсула
Original Assignee
Феб Петрольхемишес Комбинат Шведт (Инопредприятие)
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Application filed by Феб Петрольхемишес Комбинат Шведт (Инопредприятие) filed Critical Феб Петрольхемишес Комбинат Шведт (Инопредприятие)
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Publication of SU668939A1 publication Critical patent/SU668939A1/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/02—Separating microorganisms from their culture media
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02—Separation of non-miscible liquids
    • B01D17/0208—Separation of non-miscible liquids by sedimentation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01D—SEPARATION
    • B01D17/00—Separation of liquids, not provided for elsewhere, e.g. by thermal diffusion
    • B01D17/02—Separation of non-miscible liquids
    • B01D17/04—Breaking emulsions
    • B01D17/045—Breaking emulsions with coalescers

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Physics & Mathematics (AREA)
  • Organic Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Thermal Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Description

(54) СПОСОБ ВЬЩЕЛЕНИЯ ВОДНОЙ КУШЯУРАЛЬНОЙ СРЕДЫ(54) METHOD FOR CREATING WATER FOOD MEDIUM

1one

Способ относитс  к микробиологической промышленности ,а именно к способу разделени  микробиологических суспензий,полученных при культивировании микроорганизмов на углеводородах.Известен способ выделени  биомассы микроорганизмов из суспензии микроорганизмов, выращенных на углеводородах, путем декантации 1), Дл  увеличени  степени отделени  водной культуральной среда в суспензию микроорганизмов диспергируют воздух, который способствует улучшению флотировани  и отделени  микроорганизмов.The method relates to the microbiological industry, namely to the method of separating microbiological suspensions obtained by cultivating microorganisms on hydrocarbons. A method is known for separating the biomass of microorganisms from a suspension of microorganisms grown on hydrocarbons by decanting 1), to increase the degree of separation of aqueous culture medium into suspension of microorganisms air, which helps to improve flotation and the separation of microorganisms.

Однако такой способ требует значительного расхода энергии на введение в аппарат и диспергирование воздуха.However, this method requires significant energy consumption for the introduction into the apparatus and the dispersion of air.

Предлагаемый способ позвол ет увеличить ВЫХОД ВОДНОЙ среды в степень сгущени  микробиологической суспензии.The proposed method allows to increase the output of the aqueous medium to the degree of thickening of the microbial suspension.

Дл  этого процесс декантации осуществл ют с использованием контактных поверхностей в виде ЛИСТОВ, сит, колец Рашига или токарной стружки при нагрузке суспензии до 10 см на 1 см поверхности контакта в течение 1 час и скорости потока суспензии 0,5-10 см в 1 мин.For this, the decantation process is carried out using contact surfaces in the form of SHEETS, sieves, Raschig rings or turning chips with a suspension load of up to 10 cm per 1 cm of the contact surface for 1 hour and a flow rate of suspension of 0.5-10 cm per minute.

При этом концентрацию углеводородов в суспензии микроорганизмов поддерживают в пределах 5-80%.The concentration of hydrocarbons in the suspension of microorganisms is maintained in the range of 5-80%.

Контактными поверхност ми, изготовленными из стали, керамики или тефлона (политетрафторэтилена ) заполн ют аппарат-декантатор, В котором происходит разделение микробиологических суспензий.Contact surfaces made of steel, ceramics or Teflon (polytetrafluoroethylene) are filled with a decanter apparatus, in which the separation of microbiological suspensions takes place.

Способ применим дл  разделени  микробиологических суспензий, полученных при культивировании микроорганизмов на углеводородах, предпочтительно нефт ных дистилл тах, выкипающих В пределах 100-400°С.The method is applicable to the separation of microbiological suspensions obtained by cultivating microorganisms on hydrocarbons, preferably petroleum distillates, boiling away from 100-400 ° C.

Способ осуществл ют следующим образом. .Микробиологическа  суспензи  из ферментера поступает в декантатор, где раздел етс  на две фазы: легкую, содержащую биомассу, углеводороды ,воздух и некоторое количество водной среды, и т желую - водную среду. Легка  фаза декантируетс  и далее поступает на разделение из вестными способами.При этом отдел етс  дополнительное количество водной среды.The method is carried out as follows. The microbiological suspension from the fermenter enters the decanter, where it is divided into two phases: light, containing biomass, hydrocarbons, air and some amount of aqueous medium, and heavy - aqueous medium. The light phase is decanted and then fed to the separation by known methods. In this case, an additional amount of aqueous medium is separated.

Claims (2)

Заполнение декантатора контактными поверхност ми позвол ет увеличить отделение водж)й среды в процессе декантавди в 1,5-2 раза. Таким образом, получают более концентрированную легкую фазу в декантации и уменьшают нагрузку на последующие процессы сгущени  микробиологи}еской суспензии. Способ применим дл  разделени  дрожжевых и бактериальных суспензий и их смесей, полученных культивированием микроорганизмов на углеводородах. I Пример 1. В декантатор, не заполненнык контактными поверхност ми, непрерывно поступает из ферментера дрожжева  суспензи , полученна  путем культивировани  дрожжей Condida guillierucondii на нефт ных дистилл тах с пределами выкипани  220-360 С. Суспензи  содержит 2% биомассы и около 80% водной среды. В декантаторе дрожжева  суспензи  раздел етс  на слои. Нижний слой - т жела  фаза, состо ща  из водной среды, непрерывно возвра щаетс  в ферментер. Верхний слой - легка  фаза непрерывно поступает на сепарацию. В декантаторе, не заполненном контактными поверхност ми, отдел етс  41,6% водной средьь i Концентраци  биомассы повышаетс  с 2 до 3%. Пример Filling the decanter with contact surfaces allows one to increase the separation of the medium in the process of decanting by 1.5–2 times. In this way, a more concentrated light phase is obtained in decantation and the load on the subsequent thickening processes of microbiologists} of the suspension is reduced. The method is applicable to the separation of yeast and bacterial suspensions and their mixtures obtained by culturing microorganisms on hydrocarbons. I Example 1. The decanter, not filled with contact surfaces, continuously comes from the fermentor yeast suspension obtained by cultivating Condida guillierucondii yeast on oil distillates with boiling range of 220-360 C. Suspension contains 2% biomass and about 80% aqueous medium . In the decanter, the yeast suspension is divided into layers. The lower layer, a heavy phase consisting of an aqueous medium, continuously returns to the fermenter. The top layer is a light phase continuously fed to the separation. In the decanter that is not filled with contact surfaces, 41.6% of the water is separated i. The concentration of biomass increases from 2 to 3%. Example 2. В декантатор, заполненный токарной стружкой из тефлона толщиной 0,5 м непрерывно поступает дрожжева  суспензи , аналогична  по составу приведенной в примере 1. Количество отделенной водной среды составл ет 77,5%, концентравд  биомассы повышаетс  с 2 до 5,5%. Формула изобретени  1.Способ выделени  водной культуральной среды из углеводородсодержашей суспензии микроорганизмов путем декантации суспензии, о тличающийс . тем, что, с целью увеличени  выхода водной среды, декантацию суспензии осуществл ют с использованием контактных повер5сностей в виде листов,. сит, колец Ращига или токарной стружки при нагрузке суспензии до 10 см на 1 см поверхности контакта в течение 1 час или скорости потока суспензии 0,5-10 см в 1 мин. 2,Способ по п. I, о т ли   а ю щ и и с   тем, что концентрацию углеводородов в суспен-. ЗИН микроорганизмов поддерживают в пределах 5-80%. Истошики информации, прин тые во внимание при зкспертизе. 1. Патент Англии N 1177039, кл. С 6 F, 1970.2. A yeast slurry is continuously fed to a decanter filled with 0.5 m thick Teflon turning chips, similar in composition to Example 1. The amount of separated aqueous medium is 77.5%, the biomass concentrate increases from 2 to 5.5%. The claims of the invention: 1. A method for separating an aqueous culture medium from a hydrocarbon-containing suspension of microorganisms by decanting a suspension. so that, in order to increase the yield of the aqueous medium, the decantation of the suspension is carried out using contact surfaces in the form of sheets ,. Sieve, Raschiga rings or turning chips with a suspension load of up to 10 cm per 1 cm of contact surface for 1 hour or a suspension flow rate of 0.5-10 cm per minute. 2, the method according to p. I, about t and aa y u and so that the concentration of hydrocarbons in the suspension. ZIN of microorganisms is maintained within 5-80%. The sources of information taken into account during the examination. 1. The patent of England N 1177039, cl. From 6 F, 1970.
SU772504952A 1976-07-19 1977-07-19 Method of separating aqueous culture medium SU668939A1 (en)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DD19394176A DD126703B1 (en) 1976-07-19 1976-07-19 Separation of aqueous cultured liquid from biomass suspensions containing hydrocarbon

Publications (1)

Publication Number Publication Date
SU668939A1 true SU668939A1 (en) 1979-06-25

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ID=5505180

Family Applications (1)

Application Number Title Priority Date Filing Date
SU772504952A SU668939A1 (en) 1976-07-19 1977-07-19 Method of separating aqueous culture medium

Country Status (12)

Country Link
BG (1) BG35407A1 (en)
CS (1) CS196159B1 (en)
DD (1) DD126703B1 (en)
DE (1) DE2730197A1 (en)
FR (1) FR2358911A1 (en)
GB (1) GB1544374A (en)
HU (1) HU177363B (en)
PL (1) PL109681B1 (en)
RO (1) RO71482A (en)
SE (1) SE433360B (en)
SU (1) SU668939A1 (en)
YU (1) YU178977A (en)

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR1400964A (en) * 1963-03-09 1965-05-28 Metallgesellschaft Ag Method and apparatus for the separation of liquids which are not or hardly miscible
US3530039A (en) * 1966-01-17 1970-09-22 Exxon Research Engineering Co Process for fermentation and recovery of microbial cells
DE1923529A1 (en) * 1968-05-29 1969-12-04 Ingtech Zentralbuero Veb Purification of fermentation products
BE793060A (en) * 1971-12-24 1973-04-16 Metallgesellschaft Ag DEVICE FOR SEPARATING LIQUIDS
FI53075C (en) * 1973-03-07 1978-02-10 Kemira Oy

Also Published As

Publication number Publication date
FR2358911A1 (en) 1978-02-17
HU177363B (en) 1981-09-28
SE7708306L (en) 1978-01-20
BG35407A1 (en) 1984-04-15
DE2730197A1 (en) 1978-01-26
YU178977A (en) 1982-08-31
CS196159B1 (en) 1980-03-31
RO71482A (en) 1982-02-01
SE433360B (en) 1984-05-21
GB1544374A (en) 1979-04-19
PL199744A1 (en) 1978-06-05
DD126703A1 (en) 1977-08-03
DD126703B1 (en) 1980-10-29
PL109681B1 (en) 1980-06-30

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