SU1237706A1 - Stain of fungus aspergillus amstelodami using for removing retroleum products from sewage - Google Patents

Abstract

The strain of the fungus Aspergillus amste- dami G-2460 D (All-Union Collection of Microorganisms of the Institute of Biochemistry and Physiology of Microorganisms of the Academy of Sciences of the USSR, collection number VKM G-2460 D), used to purify waste water from oil products.

Description

The invention relates to microbiological activity and concerns a new strain of the fungus used in the field of environmental clean-up, in particular highly mineralized sewage and formation waters from oil and petroleum products.

The aim of the invention is to obtain a new strain of the fungus capable of a high rate of decomposition of oil and petroleum products in highly mineralized (from 50 to 200 g / l) oil-containing wastewater.

The strain of the fungus Aspergillus amsteladami G-2460 D isolated from highly unaltered (260 g / l) formation water of the Perm region, is stored in the All-Union Collection of Microorganisms of the Microorganism of the Academy of Sciences of the USSR Academy of Sciences under the number VKM G-2460 D and has the following morphological, cultural and physiological and biochemical characteristics.

Morphological and culture signs, Colonies on Chapek’s agar medium are limited to growing, 2.5–3 cm in diameter; after 3 weeks at room temperature, flat, greenish-yellow to dull yellow-gray from glutostetii, mixed with their appearance and heads with their heads, the bottom is yellowish. Cleistothecia abundant, bright yellow; conical heads dimly olive green, more abundantly develop in the center of

Research institutes, as well as scattered throughout the colony. Conidial heads radial; unpainted conidiophores, 7.8-9, O micron 8 diameter below the bladder; the bubble is oval-tarovid., 15-20 microns in diameter; fialida 4.8-6.0x3.0-4.2 microns; conidia are rough, ovate-globular 1e to elliptical, usually flat at the ends, mostly 5.4x4.2-4.8 microns. Cleistothecia is widely oval, 120-125x100-108 microns, bare; bags 8 - spore, 13.2-15x10.8-12.0 microns; lenticular ascospores, 4.8-6.0x4.2-6, 8 microns with a noticeable equatorial groove and thick scallops, with a sheath lamellar over the entire surface.

Wort agar. Colony on wort-aga-re (7 ° B) grows somewhat faster than on Capek-agar; after 3 weeks and flat at room temperature., greenish yellow to dull, from cleistothecia mixed with intraoral heads, bottom yellowish

ABOUT

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Potatoes grow well, spores.

Physiological and biochemical signs. Relation to carbohydrate pitagggo: assimilates starch, fiber, glucose, sucrose, maltose, xylose, arabinose, radium, lactose, hydrocarbons, petroleum and petroleum jelly.

Attitude to alcohols: sorbitol, man- and glycerin.

Attitude to nitrogen nutrition; Am1-Fsh-shnuyu5 nitrate and protein forms of nitrogen are used.

Milk coagulates and peptonizes on the fourth day, gelatin ie, it liquefies.

It grows in a wide temperature range of 4 icterials: 30 - 45 C, Growth Optimum i: -33 C

Optional. Aerobe. Pieces amm of the fungus Aspergillus amstelo-G-2460 D are capable of high-speed speeds and utilization of oil and oil products that are uniformly used TO U i (KoM hydrocarbon feed, in times of high mineralization waste (50-200 g / l)). At the same time, the activity of oil decomposition (by 50%) increases with an increase in the miie1) alization of the medium to 100 g / l.

Tests of destructive abilities were carried out on a hreb environment by Noreikona with the addition of Naf; l. and without salt.

1 p and me 1. In mineral medium- 4) 1 I Iorenkova s.leading composition, g / l:

(NH.) JHP04 -1

 0.2

K.; li .PQ, 0.25

0 CIRO, 0.25

0.02 0.01 0.005 Up to 1 l 5 rI7-7.2

Bocopy: and various concentrations .-: 5%, U1, 15%, 202 and without NaCl. Then the media were subjected to sternlysadia and .ppts1- and suspension of the fungus, followed by the addition; -. Of the city of Gefty in the amount of Vm, 50 ml of medium. The media without inoculants p, ii were used as a control of using the gp.

DYP testing was active and culture 5 of the fungus was cultured in TOjiMocra with 33 C I-T marked. Killed} 50% of the oil on dry matter in media with different levels of -.

The results of the experiments are given in table. one.

The duration of oil decomposition by the fungus culture on media with different mineralization is not the same.

Oil decomposition on media without salt and with 5% Nad occurred after 10% NaCl after 10% cyTj with 15% NaCl after 10-12 days; with 20% NaCl - in 15-17 days. Under the influence of culture, the oil became more viscous, its characteristic odor disappeared. The height of the oil layer was reduced by 2 times, which amounted to 50% of dry matter. In the control variants, the oil remained unchanged.

Example 2. Sterile environment of Iorena without salt and with 5% NaCl, 10% NaCl, 15% Nacl and 20% NaCl was infected with a suspension of the fungus and sterile vaseline oil was added in an amount of 8 mp

on 50 ml of the environment. The results of the experiments. given in t-abl. 2

In parallel, control was set (without inoculation). Thermostatic was carried out at 33 ° C.

The amount of vaseline oil on media with the indicated salt concentrations (NaCl and without NaCl in experimental variants decreased by 2 times, which corresponds to 50% by substance (Table 2).

The duration of the decomposition of vaseline oil culture of the fungus with different mineralization was the same as for oil.

The decomposition activity of 50% of petroleum and ground gold oil (on a dry basis) in a strain increases with an increase in the salinity of the medium from 1 to 10% g / l,

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Table 2

Claims (2)

The strain of the fungus Aereg ^ nNiz attSe1oNapp. G-2460 D (All-Union Collection of Microorganisms of the Institute of Biochemistry and Physiology of Microorganisms of the Academy of Sciences of the USSR, collection number VKM G-2460 D), used to purify waste water from oil products. one 1237706 2 Potatoes grow well, sporoobrazuet. Physiological and biochemical signs. Attitude to carbohydrate nutrition: ac5 simulates starch, fiber, glucose, sucrose, maltose, xylose, arabinose, rainoza, lactose, petroleum hydrocarbons and petroleum jelly. Attitude to alcohols: sorbitol, mannit nit and glycerin. Relation to nitrogen nutrition: use ammonium, nitrate and protein forms of nitrogen. Milk coagulates and peptoisizes 15 on the fourth day, gelatin does not thin. Grows in a wide temperature range: 30 - 45 C. Growth optimum at C = Z3 ^and C. Optional aerobic. 20 The strain of the fungus Azreg ^ Shiz atzSeXo <1 atx G-2460 D is capable of high speed utilization of oil and oil products, which are the only source of hydrocarbon feed, 25 under conditions of high salinity (50–200 g / l) of wastewater. Moreover, the activity of oil decomposition (by 50%) increases with an increase in the salinity of the medium up to 100 g / l, 30 Tests of the destructive ability of the strain were carried out on a Norenkova liquid medium with the addition of NaCl and without salt to it. ' Example 1. In mineral medium ~ 35 do Norenkova the following composition, g / l: