SU785357A1 - Micrococcus aurantiacus micrococcus rubecsens 53 species bacteria strain for purifying industrial waste water from oil and oil products - Google Patents

Description

The invention relates to the microbiological industry and relates to a new strain of bacteria capable of utilizing oil as the sole source of carbon under aeration conditions.

A known strain of Pseudomonas aefU ’g-inosa 123, used in the biological treatment of water from oil and oil products [1J.

Also known is the MicrOCO ^ CUS ¹⁰ sobro ^ bpee strain utilizing one or. * several hydrocarbons over a period of time sufficient for complete degradation of oil [2J.

A mixture of crops is also used. However, the time for which oil is disposed of is not indicated and a mixture of 16 types of cultures of various microorganisms (yeast, fungi, bacteria) isolated from non-pathogenic substrates is used.

The aim of the invention is a strain of hydrocarbon-oxidizing bacteria capable of oxidizing oil as the sole source of carbon and energy under aeration conditions.

Strain Micrococcus aurantia cus bar. M.rubecseu5 53 is characterized by the following features.

Morphological signs. Spherical cells are also oval, usually single, as well as two, four connected in clusters and in short chains, ranging in size from 0.5 to 1.25 microns in diameter, motionless.

Meyeopeptic agar - when inoculated on a liquid mineral medium with oil on MPA, small 1-2 mm in diameter pink or light red colonies grow, and with aging orange-red color, shiny, convex, the edges are even, easily removed by a loop. · Pigment is not form. Grow with subsequent reseeding on the MPA. On potatoes, growth in the first three days is weak, and then good, the color is brown-red.

Physiological signs. Aerob, optimum growth 27-28 °, maximum growth. 37 ° C. It does not dilute gelatin, milk does not clot, and grows well on synthetic media. Starch does not hydrolyze.

Absorbs mineral nitrogen, peptone. Nitrates reduces to nitrites.

Attitude to carbohydrates and alcohols.

Does not absorb glucose, sucrose, tzu, arabinose, lactose, sorbitol nit.

Mali May — with crude oil on mineral medium as the sole source of carbon and energy, forming a plentiful turbidity, film. The bacterium is able to oxidize 86.1% of oil in one day of incubation, while in the chemical control (medium without the addition of bacteria) the natural decrease in oil was 1.5%.

. The strain was isolated from a soil medium ¹ therein the Caspian Sea, the station 727 with a depth of 600 m on a mineral oil with a liquid medium. Further, when sifting culture on MPA and repeat. ·. When it was tested on a liquid mineral medium with oil, they again discovered the presence of a bacterial film, turbidity, and the absence of oil.

The culture of the strain WCPOCOCCUS aur'an-U'acus bap. Micrococcus is stored in the collection of the Institute of Microbiology and Virology of the Academy of Sciences of the Kazakh SSR, No. 28.

An example of a new material.

1000 ml of medium

N n ₄ s O _£ : K ₂ NRA ₄ kn ₂ ro ₄ M {5SO ₄ '7H /) O CaCl ₂ -6H ₂ O ReCl _{1 3} -6H ₂ O • 4

After 24 hours, the oil disappears, an abundant opacification forms, a bacterial film.

The quantitative consumption of oil by microorganisms is determined by the luminescence-chromatographic method based on the chromatographic separation of hydrocarbon oil in a thin layer of alumina and its subsequent 10 luminescent and spectrophotometric determination.

Thus, the MicroCOCCUS auromti acus bap.M.rubecsens 53 strain is capable of utilizing up to 86.1% of oil in one day of incubation, while the natural decline in oil in the medium without the addition of bacteria was only 1.5%. ... - ·. ·· '

A bacterial strain is capable of utilizing oil as the sole source of carbon and energy in the absence of organic matter, can be used in the purification of waters contaminated with oil, and is promising for the biological purification of water from oil and oil products.

Claims (2)

37 Physiological signs. Aerobe, optimum growth 27-28 I maximum growth 37 C. Gelatine does not thin, milk does not coagulate, grows well in synthetic media. Starch does not hydrolyze. Absorbs mineral nitrogen, peptone. Nitrates restores to nitrites. Attitude to carbohydrates and alcohols. Does not digest glucose, sucrose, maltose, arabinoau, lactose, sorbitol and mannitol. It grows on a mineral medium with crude oil as the sole source of carbon and energy, forming an abundant clouding film. The bacterium is capable of oxidizing 86.1% of oil per day of incubation, whereas in chemical control {medium without adding bacteria), the natural loss of oil was 1.5%. . The strain was isolated from the soil of the middle part of the Caspian Sea, from station 727 from the depth of the sea in the mineral liquid medium with oil. Later, when the culture was sieved on MPA and re-tested on a liquid mineral medium with oil, the presence of a bacterial film, turbidity and the absence of oil were again detected. Culture of strain M SGCPCPUS durbn-tmcus bap. Mfcnococcus is stored in the collection of the Institute of Microbiology and Virology of the Academy of Sciences of the Kazakh SSR, No. 28 .; Example. Getting seed. 1000 ml of medium containing, g / l: N O -1.0: K2HPb41.0 KH2PO41.0 Mb5047NlO 0.2 - - 0.02 CaCl -eH.jO (saturated solution 2 drops Liquid hydrocarbon; 1 ml sterilized at 1 atm 30 min. The medium is poured into the flask at 2OO ml each, seeded at the rate of 5% bacterial suspension, which is 10 to the current. Oil is sterile 0.1% of the volume of the medium and placed on a rocking chair (180 rpm The experiments were carried out in two replications: one biological miner on culture medium, no oil; and another chemical mineral medium and oil, no bacteria. After 24 hours, disappears n An abundant coagulation, bacterial film is formed, quantitative oil consumption by microorganisms is determined by a luminescence chromatographic method based on chromatographic separation of hydrocarbon oil in TJOHKOM alumina, followed by its acidic and spectrophotometric determination of the alumina. rubecsens 53 is capable of utilizing up to 86.1% of oil per day of incubation, whereas in a medium without the addition of bacteria, the natural loss of oil was only 1.5%.: .., -. The bacterial strain is capable, in the absence of organic matter, of utilizing oil as the sole source of carbon and energy, can be used in the purification of waters contaminated with oil, and is promising for the biological purification of water from petroleum and petroleum products. . M CY OCOCCUS PUbecSeus 53 used to treat industrial wastewater from oil and oil products is stored in the collection of the Institute of Microbiology and Virology of the Academy of Sciences of the Kazakh SSR, No. 28. Morphological features. The cells are spherical in shape, less often oval in shape, and also connected in two, four in clusters and in short chains of 0.5 to 1.25 microns in diameter, fixed. Gram-positive. / M septarine agar - when sowing on liquid mineral medium with oil, small 1-2 mm in diameter grow colonies, pink, light red, and with aging orange-red color, shiny, convex, smooth edges, easily removed by a loop. Pigment does not form. Grow with the subsequent reseeding on MPA. Physiological signs. Aerobe, optimum growth of 27-28 C, maximum growth of 37 ° C. On potatoes, growth in the first three days is weak, and then a good, brown-red color. 5 tesss Gelatin is not diluted. Milk does not roll. Krakmal does not hydrolyze. It grows well on synthetic turtles. Absorbs mineral nitrogen, peptone. Nitrates reduces to nitri-5 tov. Does not digest glucose, sucrose, arabinose, lactose, sorbitol and mannitol. It grows on a mineral medium with no mineral as the only source of carbon, Q of the genus and energy, forming a rich, cloudy. film, film. 6. Bacteria are capable of oxidizing 86.1% of oil per day of incubation, whereas in chemical control (medium without the addition of bacteria), the natural loss of oil is 1.5%. Sources of information taken into account in the examination 1. USSR author's certificate N9 612958, cl. From 12 to 1/02. 1976. 2. Patent of France. N 2095822, class From 02 to 9/00, 1972.