WO1995031408A1

WO1995031408A1 - Biological preparation and method of removing crude oil and petroleum-product contaminants from water and soil

Info

Publication number: WO1995031408A1
Application number: PCT/RU1994/000103
Authority: WO
Inventors: Igor Anatolievich Borzenkov; Sergey Semenovich Belyaev; Ravil Rustamovich Ibatullin; Mikhail Vladimirovich Ivanov; Evgenia Ivanovna Milekhina; Nelya Abdullovna Roschektaeva
Original assignee: Aktsionernoe Obschestvo Zakrytogo Tipa 'biotekhinvest'
Priority date: 1994-05-11
Filing date: 1994-05-11
Publication date: 1995-11-23

Abstract

The proposed biological preparation for removing crude oil and petroleum-product contaminants from water and soil consists of a mixture of the following hydrocarbon-oxidising strains of micro-organism: rhodococcus sp. BKM-AC-1500 D, rhodococcus maris BKM-AC-1501 D, rhodococcus erythropolis BKM-AC-1502 D, pseudomonas stutzeri BKM-B-1972 D, Candida sp. BKM-Y-2778 D. The relative proportions of these strains are (0-1):(0-1):(0-1):(0-1):(0-1). The prepar ation in question is a naturally occurring association of microorganisms or mixture of individual micro-organism strains. The proposed method of removing crude oil and petroleum-product contaminants from water and soil involves treating the water or soil with the biological preparation containing the strains rh. sp. BKM-AC-1500 D, rh. maris BKM-AC-1501 D, rh. erythropolis BKM-AC-1502 D, ps. stutzeri BKM-B-1972 D, and C. sp. BKM-Y-2778 D. The preparation used contains no fewer than 2 x 1013 live microbial cells for every square metre of contaminated area. Treatment of the water and/or soil is carried out by sprinkling a suspension of the preparation in a solution of mineral salts. For the treatment of soil, trenches are cut in the soil. Water and/or soil with a salinity of up to 10 % is treated in this way. The preparation can be used for treating reservoirs and saline soil and brackish water ecosystems.

Description

Biological product and method of water and soil treatment from pollution of oil and oil products

5 TEXT TECHNOLOGY

The invention is related to microbiology, ecology, environmental damage, and more precisely, concerns about the environmental costs of the environment. 10

The foregoing level of technology.

Increasingly, the volume of production, processing, processing and use of oil is neglected by the ubiquitous industry.

15 contamination of stocks and soils with crude and non-processed foods. Ηesmοτρya on πρimenyaemye πρedοsτοροzhnοsτi on neφτeπe- ρeρabaτyvayushiχ zavοdaχ, liniyaχ τρubοπροvοdοv, neφτyanyχ τan- κeρaχ πeρiοdichesκi sluchayuτsya avaρii, ρezulτaτοm κοτορyχ yav- lyayuτsya chρezvychaynο οπasnye for οκρuzhayushey sρedy προlivy neφ-

20 and non-product emissions.

It is known that in practice there is an efficient system of processing of hazardous waste, which is associated with the disposal of a lot of carbohydrates. One of the microbes is no longer in the state of disruption of tens of millions of tons.

25 And, every day, they are falling into a shrinking environment. Β ρezulτaτe che lοvechesτvο οκazalοs πeρed ρealnοy ugροeοi eκοlοgichesκοy κaτasτροφy (^. Κ. Βgο \ vn Seshϊsa Ι Εηβϊηeegχηβ Ρgο £ ge5≤ 1987, νοϊ. 83, N 10, ρ. 35-40 "0ϋ-άe £ gaάϊη§ shχsgοοgβaηϊzshz" )

What are the traditional methods of calculating, as mechanical,

30 Chemical and physical do not ensure the required level of water and soil are not contaminated. Otherwise, in many cases, the use of these methods will cause a more significant damage to the environment than non-active consumers themselves.

35 Β In connection with this, it is obvious that it is not necessary to work out environmentally friendly, non-hazardous methods of calculating, including the fact that it is not used. - 2

Depending on the structure of the cellular wall, the microorganisms can be divided into two groups: lipid and. hydrophilic microtrophy.

In case of a biphasic system of the type 'water-hydrocarbon'

5 or “water-in-oil” lipid microorganisms are concentrated in the hydrocarbon phase (inactive), and hydrophilic ones are in the aqueous phase. specific micro-gangnisms can be developed only slightly in the case of a slower film, which significantly complicates the process of calculating water and negligence. Οdna-

10, partly the components of the oil are disposed of in the water, and this contamination is eliminated by a very small, slower one.

The difference is that there are many, flexible, multiple mechanisms that can be used to utilize only connected connections. 15 The lack of water in the water is low, and the use of a hydrogen-free culture means that there is no need to increase the cost of the load. The speed of the process in this case is limited by the speed of the diffusion of inactive products and the contact area of 20 tons. For thicker films greater than 1 mm, it is inconsequentially unacceptable.

Putidoyl biological products are known (Α. S. CCC С 1076446,

1982, Α. from . СССΡ Ν 1428809, 1988), created on the basis of the microorganism of the Republic of Belarus, "Bioprin" (oleurin; ΤУ

25 1 1279895-12-22-92) used to calculate water and soil from uncontaminated pollution.

These biological products are produced on a basic multiplicity of a bacterium of a hydraulic group. Accordingly, each of them, to the same extent, is inherently deficient in the foregoing. 30 Alternatively, biocompatible products operate on a narrow range of ρΗ and are capable of active oxidation of carbohydrates. Oxidation is associated with hydrocarbons with a long range of C, and heavier, heavier components are left unavailable for minus 35 cultures.

The product is known to be free from a mixture of non-oxidizing multiple organisms with nitrogen compounds - 3 -

φορа, neobkh ^χ acceptable to them (ет ο а а ά

»Е »5, 1970, νοϊ. 48, ρ. 48).

Pρeπaρaτ πρigοden for οchisτκi vοdy and ποchvy οτ uglevο- dοροdοv with dlinοy uglevοdοροdnοy tseπi not πρevyshayuschey C ^ 5 Izvesτny biοπρeπaρaτy, πρimenyaemye for οchisτκi οτ neφ- τyany ^χ zagρyaznenin vοdnοy ποveρχnοsτi (Paτenτy SSHΑ ΝΝ 3,843,517, 3,871,957, 3,959,127), οchisτκi sοdeρzhashiχ neφτ sτοchnyχ vοd (Nov. ССС τ ρ 793375, 823314, 925875, United States Patent No. 4288545, 4452894, 4535061, 4593003, 4732680), ДΛЯ

10 removal of petroleum contaminated soils (US Patent Nos. 4136024, 4452894, 4521515, 4535061).

However, the degree of primary water and soil due to hazardous contaminants using known biological products is not satisfactory. Τ ο ο τ τ τ,, и,,

15 counts of populated soils and salt-based ecosystems.

It is also necessary to note that the use of biological products containing carbohydrate-oxidizing substances for the treatment of water and / or any other discomfort

"0 Izvesτnο, chτο uglevοdοροdοκislyayuschie baκτeρii yavlyayuτsya aeροbnymi, τ.e. for iχ zhiznedeyaτelnοsτi neοbχοdim κislοροd. Κροme τοgο, miκροορganizmy nuzhdayuτsya in azοτe and φοsφορe. Without κislοροda, azοτa and φοsφορa miκροορganizmy not οκislyayuτ carbon vοdοροdy and sledοvaτelnο not mοguτ οchishaτ vοdu and / or soil

25 of oil and non-food products.

Βnοsnmye in ποchvu baκτeρii πρi presence κislοροda, azο- τa, φοsφορa in προtsesse svοey zhiznedeyaτelnοsτi πeρeρabaτyva- yuτ uglevοdοροdy dο malοτοκsnchnyχ or bezvρednyχ κislοροdοsο- deρzhaschiχ sοedineny vπlοτ dο ugleκislοgο gas liκvidiρuya

30 Such an environmentally hazardous pollution.

In other slοvami, πρimenenie uglevοdοροdοοκnslyayuschiχ miκ- ροορganizmοv for οchisτκi vοdy and / or ποchvy οτ neφτi and neφτeπ- ροduκτοv mοzheτ byτ οsuschesτvlenο οπρedelennym sποsοbοm in κοτοροm buduτ uchτeny οsοbennοsτi φiziοlοgii mnκροορganizmοv.

35 Izvesτen sποsοb lnκvidatsii neφτyanyχ zagρyazneny ποchvy, vκlyuchayushny ρyχlenie ποchvy vsπashκοy with a view to its aeρatsii, extra- senie it sπetsialnοy κulτuρy baκτeρy and mineρalny ^χ sο- Leu (Ρeκulτivatsiya lands zagρyaznennyχ neφτyu. EI seρ. Za- _ 4 -

a shield for rest and lack of environment 1991, v. 6, C 52-56).

However, the depletion of the soil does not make the soil air, depending on the degree (depth) of its pollution with 5 lodges and disruptions.

In general, the problem of the removal of oil and inert products from the passage of water and / or soil is that the product is inactive and is inoperative. Another problem is the calculation of water and / or soil in a populated area. This problem did not find a solution to it.

DISCLOSURE OF INVENTION.

fifteen

Β οsnοvu izοbρeτeniya ποlοzhena task - sοzdaτ biοπρe- πaρaτ, aκτivnο οκislyayuschy neφτeπροduκτy κaκ in zοne κοnτaκτa with vοdοy, and τaκ neποsρedsτvennο in neφτyanοy πlenκe, sποsοbny uτiliziροvaτ shiροκy diaπazοn uglevοdοροdοv neφτi κaκ 20 πρesnοy, and ρ τaκ zasοlennοy eκοsisτeme and τaκzhe sοzdaτ sπο- Calculation of water and / or soil due to hazardous contaminants using this biological product.

The task posed is solved by the fact that a biological product has been created, as a result of which there are both typical, and hydrophilic 25 microorganisms.

When using the product, all the above-mentioned disadvantages of the application are eliminated if the biocomputer is loaded without a load on the main load.

The biological product, in accordance with the invention, is supplied with 30 special small products, which is a result of the loss of public health, which is a part of the world-wide trade in goods.

A successful combination of components delivers a biological product in the range of benefits from other non-food products. 35 Organisms that are part of the biological system, are capable of disposing of non-hazardous carbohydrates. In other words, the biological product is suitable for use in the case of ^χ , when - 5 -

Contamination is a significant part of the net and oil products of a significant thickness.

The biological product is stable to other temperature fluctuations and can be used for the treatment of water and / or soil at a low 5 and high tempera- ture.

The biological product is adapted to the environment with high salinity (up to 15% of the land) and, as a result, it is calculated for calculating salaries and salaries.

Ав With the biological product included 10 bacterial and yeast cells. Bacteria are supplied with a variety of bacteria of the genus Bacteriosis and hydrophilic bacteria of the Zeus erychosphenium. Yeast cells are provided in it by the strain Saηάχάa ∑> ρ.

The first association of microorganisms is distinguished from the plastered territory of the country by the following method. The 15th place of business water is placed in a food that contains oil in the amount of organic matter and is cultivated for 30 days at a temperature of 28 ° С. Then, by the way, dozens of reciprocal dilutions isolate microorganisms that are capable of disposing of non-productive products. 20 The Natural Association of Microorganisms, isolated from industrial waters; denia, is stable at the same time, τ. e. there is no crowding out (loss) of its separate components, a simple association is a stable, incidental to the incidents of mediations.

The biological product can be manufactured in two ways.

Peρvy sποsοb zaκlyuchaeτsya in τοm, chτο baκτeρialnye κοmποnenτy, sοsτavlyayuschie πρiροdnuyu assοtsiatsiyu, vyρaschivayuτsya in προmyshlennyχ φeρmenτeρaχ οτdelnο with ποsleduyushim iχ mixing 30 the obtainable nuzhnyχ προπορtsiyaχ and drying πο sπetsialnοy τeχnοlοgii dο moisture sοdeρzhaniya not bοlee 10%.

The short-range way to support the joint cultivation of the gamma of the microorganisms. At the same time, the suspension of microorganisms in suspension is achieved by the Regulatory Regulatory System 35 of healthy food.

The relationship of different microorganisms in a biological product is subject to change, in all conditions it will be used. - 6 -

Τa, πρi πρnmenenii biοπρeπaρaτa for οchisτκi κislyχ ποchv πρeimuschesτvennο nsποlzueτsya mixture miκροορganizmοv κοάοsοssiδ 5p., Κοάοsοssiz gaagϊ-W Κοάοsοssiδ eguΙgοροϋδ, Ρzeiάοshοηaδ δϊiϊζegχ, Saηάχάa zρ. in fact, 5 days are 0.1: 0.1: 0.1: 0.1: 1, and in the silk conditions, another ratio of microorganisms is recommended 1: 1: 1: 1: 0.4, respectively.

Ηezavisimο οτ sποsοba nzgοτοvlennya οsnοvnaya οsοben- nοsτ πρeπaρaτa sοsτοiτ in τοm, chτο miκροορgannzmy, vχοdyaschne 10 egο sοsτav, mοguτ ρazvivaτsya in shiροκοm diaπazοne sοlenοs- τn (0.05-15%) and from ποeτοmu zamenοy κοmποnenτοv πρeπaρaτa non izbezhnο reduction sledueτ Its efficiency, especially in applications in populated systems.

15 Description of the strains of microorganisms on the part of a biological product

Shtamm Ρzeiάοшοηaδ δϊиϊζегϊ ΒΚΜ-Β - 1972Э

20 Allocated from the industrial waters of the Bonduzhsky non-affection ~ at the place of birth.

1. Medical Signs. Large, movable fingers. Size plet

25 1.5 χ 0.7 μm.

2. Cultural Signs.

The rooms on YPP are light gray, round, with a diameter of 3 to 15 mm, almost flat, in the main part, they are generally used. 30 Territory complete, casual. In the case of a large agaric, light-gray, large, round, convex with the right edge, the central part of the beige tone, tuberous. Δ and diameter τ 1-3 mm. The system is soft, easy to remove from the agaric, and easy to spread. Fluorescent pigment does not work.

35

3. Physiological Signs.

The positive, metabolic dyspeptic type. Ω - arginn not used. - 7 - It is lost on the company, acetate, acetate, butarate and low molecular weight (excluding methane), the metal is absorbed. They reduce glucose, маль-maltose, E-arabinosis and ла-lactose, but the accumulation of biomass is weak. Hydraulics 5 κρa ^χ small. They are well-suited and are suitable for the environment in the multi-media environment of Limend (with 1% of the acetate and 0.25 of the burning extract), which contains up to 15 ° C. Use η-alkanes

-butrates do not accumulate. It uses ammonium and nitrate-free nitrogen, nitrates, and nitrogen does not.

10

4. SECONDARY CHARACTERISTICS. The optimum temperature of the plant is 30-32 ° С. θπτtum ρΗ - 6.8-7.2. Not pathogen.

fifteen

5. Strain removal.

The strain is stored in a collection in the United Kingdom. To save the strain, use the method of conversion (3-4 times a year) to the plant or to the agaric with

20 1% NaC. Incubation after depressurisation leads to a temperature of ϊ & ^α C for 3-5 days, then the culture is kept in a cold store

: e πρ and temperature 4 ° С.

The Storm Vkm ΑС-1500Ρ

25

It is isolated from the industrial waters of the Bonduzhsky non-land plot.

1. φ ρ φ φ φ на на на на.....

30 Grammar, fixed sticks. The one-sided culture on the mineral medium of Oymanda (with 1% of CACC, 1% of acetate and 0.25% of direct extract) is inactive. When dividing, there is a wise disposition of the cell when it is angled to the other. Size ρ cell - - 2.3 χ 0.6 μm, s

35 years ago, a shortening of the braids is observed.

2. Cultural Signs.

It has type IV cell walls and contains acid and phosphodieslamine. - 8 - Lines on a hot agaric are orange, orange, 1-1.5 mm in diameter, convex with an alternate edge, glistening. The onset is soft, easy to remove from the agaric, and easy to spread. There is a negative impact on the agro-friendly medium of Caimend 5 with acetate and hexadecane in the quality of an organic substrate. It is well-tempered, and is suitable for mineral production of lime (with ι% acetate and 0.25% alkaline extract), which accounts for 15% of the environment. With a salinity of more than 5, there is a slowed-down development of small ones with the development of a small amount of food, 10 to a small amount of orange.

3. Physiological Signs.

It is non-hazardous, non-solvent, gelatin is not liquefied, casein is not degradable, it is not hydrated. 15ο- 15 have assimilated η-alkanes (C < _ύ -С ₃₀ ), acetate, butyrate, glucose, маль-maltose. Weak development on industrial, business, ethanol, industrial panel, butanol. It does not assimilate 0-arabinosis and ла-lactose. It has a good effect on yeast. Ammonium and non-nitrogen are used.

20

4. The economical type of technology. The optimum process temperature is 30 ° - 32 ° C. The maximum temperature is 6.8-7.2.

25

5. Strain removal.

The strain is collected in a collection in a licensed community. In order to save the strain, they use the method of conversion (3-4 times a year) on a crop agar with 1% SAS. 30 incubation after depressurization leads to a temperature of 28 ° C for 7 days, then the culture is refrigerated at a temperature of 4 ° C.

35 Stamm м Ь ά ά с ϊ ϊ ϊ ΒΚΜ Α-C-1501 Ο.

It is isolated from the industrial waters of the Bonduzhsky non-land plot. - 9 -

1. φορ ρ φ че з з з з Signs

Large, fixed, very small with carbonized ends of the stick. Β οdnοsuτοchnοy κulτuρe on mine- ρalnοy sρede Ρaymοnda (s ΝaSϊ ι%, ι and 0.25% atseτaτa dροzh- 5 zhevοgο eκsτρaκτa) πρeοbladayuτ neρazοshedshiesya in χοde dividing κleτκi with χaρaκτeρnym ρasποlοzheniem ποd uglοm dρug κ dρugu. The size of the cells is 1.3 χ 0.8 μm; with the increase, their shortening is observed.

10 2. Cultural Signs.

It has an IV type of cell wall and contains acid and phosphodieslamine.

On the farm are large orange, small, 1-1.5 mm dia., Convex with a sharp edge, shiny. Κοн-

15 is soft, easily removed from the agaric, and easy to spread. It spoils the agarized environment of Khaimand with acetate and hexadecane in the quality of the organic substrate. It is well-tempered, and is suitable for mineral processing of lime (with 1% of acetate and 0.25% of burned extract), it is friendly

20 living for 20% ΝаСϊ. At a salinity of more than 5%, there is a slower pigmentation of the growths with the development of a small amount of weak orange.

3. Physiological Signs.

25 Non-hazardous, non-acid-resistant, do not liquefy gelatine, do not decompose casein, does not hydrate.

They assimilate η-alkanes (С ^ -С- ^), acetate, propane, butyrate, ethanol, butanol, glucose, glucose. Weak development on the economy and methane. Good for business

30 extracts. Ammonium and non-nitrogen are used.

4. SECONDARY CHARACTERISTICS. The optimum temperature of the discharge is 30 ° - b2 ^С С. θπτtum ρΗ - 6.8-7.2. Home Development 6.2

35 to 9.2.

Not pathogen.

- 10 -

5. Strain removal.

Shτamm sο ^χ ρanyaeτsya in κοlleκtsii in liοφiliziροvannοm sοs- τοyanii. for sο ^χ ρaneniya shτamma isποlzuyuτ meτοd πeρiοdnchesκiχ πeρesevοv (3-4 ρaza in gοd) on κaρτοφelnοm agaρe 1% ΝaSϊ. 5 Incubation after depressurization leads to a temperature of 28 ° C for 7-10 days, then the culture is refrigerated at a temperature of 4 ° C.

10 Stamm of the World War II. ΑC-1502 0.

It is isolated from the industrial waters of the Bonduzhsky non-land plot.

15 ι. logical signs.

Grammar, stationary. With the development of a well-known change in the type of culture of the type "kokka-stick-kokka".

The one-sided culture on the mineral medium of Oymanda (with 1% NaCϊ, 1% acetate and 0.25% acid extract) is directly 20 with carbonated stones. When dividing, there is a rough disposition in front of a corner to a friend. The size of the glue οκ is 2.7 χ 0.7 μm; with the increase, the shortening of the cell to 0.8 χ 0.6 μm is observed.

25 2. Cultural Signs.

It has an IV type of cell wall and contains acid and phosphodieslamine.

The pan on the agar is beige, round, diameter up to 5 mm, convex with an ordinary edge, glistening, mucous

30 th. The structure is mild, easily removed from the agility of the agar, easily spread out. It has a negative impact on ΑPΑ, must-agar and on the agri-enriched environment of Khaimand with an acetate and hexadecane in the quality of an organic substrate. Β Liquid environment is rich in mucous sediment.

35

3. Physiological Signs.

It is non-hazardous, non-solvent, gelatin is not liquefied, casein is not degradable, it is not hydrolyzed. - eleven -

Well assimilated η-alkanes C _{/ 0} -C ^, acetate, butnate, ethanol, butanol, glucose, маль-maltose, ла-lactose. Weak development on social media, social media, social media, а-arabinose. It has a bad effect on the yeast extract. It is well-tempered, and is suitable for 5% of the mineral content of мммдада с (((from 1 acetate and 0.25% of burning extract), which comprises 15% of ϊаСϊ. Ammonium and non-nitrogen are used.

4. Non-medicinal design.

10 Optimum temperature of the circuit - 30 ° - 32 ° С.

Οπτimum ρΗ - 6.8-7.2. The potential development is 9.4. Not pathogen.

5. Strain removal.

15 The strain is collected in a limited society. For storing the strain, use the method of conversion (3-4 times a year) to ΜPΑ, wort or short-term agar with 1% ϊаСϊ. Incubation after reseeding leads to a temperature of 28 ° C for 3-5 days, then the culture is taken in

20 ° C. - Only at a temperature of 4 ° С.

Stamm Saηάιάa ≤ρ. ΒΚΜ Υ-2778 Ο.

It is isolated from the industrial waters of the Bondyuzha non-ferrous locality - 25 birthdays.

1. φ ρ φ φ φ на на на на.....

Д one-sided culture on the mineral medium Ρaimanda (with 1% of the United Arab Emirates, 1 acetate and 0.25% of the greenhouse); mkm

2. Cultural Signs.

There is no reason for the eruptions of the structure (neither ascension nor basidi- 35 species).

There is a good result on the agarmed environment of Kimond (from 1 percent and 0.25 burnt extracts), which accounts for up to 8% of SaCϊ.

- 12 -

3. Industrial Signs.

They assimilate η-alkanes (C < _c -C _ze ), acetate, and other. Weak development on ethanol, προпаноле. They don’t dump glucose, they don’t assimilate it. Ammonium nitrogen is used, 5 tests for the urine urinary tract.

4. SECONDARY CHARACTERISTICS. The optimum temperature of the temperature is 30-32 ° С. θπτnmum pH - 5.5-6.0. Interdevelopment πρ and ρΗ -

10 3.0-3.5.

Not pathogen.

5. The previous strain.

The strain is collected in a fully-functional system.

15 points. In order to save the strain, use the method of conversion (3-4 times a year) on the ΜPΑ, wort or short-term agaré with ι% ΝаСϊ. Incubation after depressing takes place at a temperature of 28 ° C for 3-5 days, then the culture is stored in a refrigerator at 4 ° C.

20 for the preparation of the product in accordance with the invention, the indicated strains of the mixture and the other are mixed. Big deal can be different for individual strains. The preferred ratio is the equal amount of each strain in the mixture. End of life

25, depending on the conditions, in which case a biological product is used (selenost, degree of pollution and t.pi.).

The use of a biological product does not depend on the method used to produce it.

If the biological product is used for soil, then for

30 aeration of the soil in it places the waste, waste and the frequency of disposing of the waste depends on the degree of pollution of the soil with hydrocarbons. The flow of water to the former is a suspension of the microorganisms and minerals that they collect and, if necessary, use a large and non-expensive suspension.

35 Facilities for the addition of nitrogen and phosphorus.

The biological product is efficient and quick to dispose of in a contaminated environment while adding nitrogen and mineral salts. The required amount of mineral minerals depends on the total amount of contamination.

- 13 - On the basis of the quality of our vegetables, diamphorus, ammophus, various spices, nardarme, salt and minced meat, may be used, . 5 On the other hand, the various types of phosphates may be used, for example, the odds are too small or too small:

The most preferable source of nitrogen and phosphate 10 is a mixture of diameters ((ΝΗ) ΗΡΟ.) - ΔΑΦ and ammonium sulphide (ΝΗ ^ Ο ^) - н -: (D): 10 -20).

Typical ratios of the carbon-contaminant (C), nitrogen (Ν) and phosphate (Ρ) - C: Ν: Ρ = 100: 10: 1. The initial cost is 15 charity of microorganisms, which is necessary for the quick development of the biological process, which makes 10 - a free battery for 1 product.

In general, for quick and efficient disposal of 1 tonne of crude oil, you need to use it: 20 kilograms of crude 1000 biomass, οι - 1.0

Ν 100

Ρ 10.

25 best options for carrying out the invention

The best price for the product recommended for the calculation of neutral soils is that δδ. ΚΚάάссοοсссиз т δχδδ, ,ΚΚΚΚΚΚΚΚсссссссеггггггггггггггггггггггггггггггггггггггггггггггггггггггг ,г, ,ггг,,,,,,,,,,,,,,,,,,, ,г,,,,,,,,,,,,,,,,,,,,,,,,,,

30 δϊиϊζегχ, Саηάχάа 5р. at a ratio of 1: 1: 1: 1: 1 and a mixture of ΔΑΦ with вС in a weight ratio of 1:14. The content of vital cells in the unit is 4 x 10 cells / g dry weight. The ratio of the biological and mineral components is 1 to 3,000.

The stated offer may be prepared in advance.

35 solid mixing of dry biological product, D ,Φ and ΑС. In this case, the com- pensation makes it possible to get a light and beautiful color (humidity no more than 10%) with a content of live cells of 1.33 g / y. - 14 -

The indicated specification is recommended for the calculation of uncontaminated pollution due to the following: biopreparation: mineral salts equal to 1: 0.0001: 0.3.

The high ratio of biomass and net is the most effective 5, which is illustrated in table 1.

Table 1.

Degree of completeness of productiveness of desserts, Efficiency, dirt, biological product, oil products, hour 10 kg non-meter / sq. g / sq.m g / sq.m hour

Α C Β ϋ

10 0.1 0.05 20,000

15 10 1.0 1 10000

10 10.0 2 50000

EFFICIENCY ϋ =

indicates the time for which

20 unit of weight of the preparation (1 g) oxidizes the unit of weight of the net

(1 kg) on the first square meter.

The following are examples of the calculation of the soil, water, and va-ura in accordance with the invention.

25 EXAMPLE 1. Soil calculation.

1. Preparation of the foreign land.

They take into account the area of the contaminated area, the level of soil access, the depth of the access to hydrocarbons in the soil, and the average amount of carbohydrates is 30%. They calculate the amount of air needed for the depletion of hydrocarbons for SJ ^, based on this, we choose the size and the rate of use of the trap.

2 Table 2 shows the parameters of the two sections with a different degree of pollution. - fifteen -

Table 2

ΝΝ PARAMETER PART I PART II

1. The length of the contaminated area, m 10 10

2. The width of the contaminated area, m 10 10

3. Area of the contaminated area (5), sq.m 100 100

10 4. HUMANITY OF SOIL (W), CU 0.25 0.25

5. Depth of hydrocarbon production in soil (b), m 0.3 0.5

6. The average molecular weight of hydrocarbons (ρ), κ. 200 200

15 7. Saturation of soil with hydrocarbons (5 nn), 0.1 0.5

This follows from the standard calculations for the oxidation of carbon dioxide of 1 square meter of hydrocarbon with a molecular weight of 20 weighing 12 thousand. cubic meter of air.

Pρi ρas ^ Te οbema τρanshey sledueτ uchesτ κρaτnοe An increase aeρats br zε scheτ tsiκlichnοsτi προtsessa (τ.e. chislο tsiκlοv isκussτvennyχ) and τaκzhe dοποlniτelnuyu aeρatsiyu, ρav- hydrochloric isκussτvennοy, on account esτesτvennοy tsiρκulyatsii vοzduχa 25 dlinnyχ τρanscheyaχ (chislο Natural Cycles). In addition, aeration also increases due to the exchange of air.

Assume the number of valid cycles (η ^) = 100. Of course, the number of valid cycles ( ^η special) = ^1υ0 - ^{At the} expense of 30 augmentation of a bus charge. Taking into account these factors, the volume of air used for the oxidation of 1 cubic meter of hydrocarbons in one cycle is 12,000 cubic meters. = 0.6 cubic meters

35 100 - 100 • ”2

- 16 -

The volume of hydrocarbons (V / in) is calculated on the basis of the following formula:

100 ^' • 0.3 for section IV ¹ y / v = »ο, ι = 12 m3

0.25 5 100 • 0.5 for section II ν-у в = .0.5 = уο м.уб.

0.25 Air volume (ν _Λ ),), suitable for the oxidation of hydrocarbons, respectively: 10 V ¹ Д03Β. = 0.6 • 12 = 7.2 (уб cub.) Ν "ΒΟ ЗЗ. = 0.6.100 (ubub.m)

By assuming that V inlet = ν τ is more = 1 - b -, where 1, b and b are the corresponding bus, the depth and length of the tube, given the width and depth, are taken into account by taking the distance into account.

In this particular case, the width of the trench is 0.15 m, the depth for section I is 0.3 m, for section II it is 0.5 m. When 7.2 60

B = 160 m; - = = 800 m

20 ^" 0.3 • 0.15 ^" 0.5 • 0.15

In section I, follow ': pay 16 rubles, and in section II - 80 rubles, then the length of them is calculated net, and therefore, it is only 8, if you pay 8, pay 8.

25 meters with a frequency of 1.25 m; at section II - 40 in the distance, 40 part-time with a frequency of 0.25 m.

In addition to the above analyzes, measure the angle of the tax on the foreign area. If it is equal to zero, then the depth of the road at the length of the road increases the length so as to create a slope of 30 2-4 ^* .

2. Use of the machine.

A mixture of the strains , κЬάάсссссссδδ га гааг ,зз, ,ΚΚΚΚάάсссοсссс егегггггггггρρ,,, ,ΡΡΡΡάάгшϊϊϊϊ Са Са Са Са Са Са Са Са Са Са Са 5 5, 5th at a ratio of 1: 1: 1: 1: 1 they are cultivated in liquid nutritional range of 35 days, which contains a source of nitrogen, phosphate, microelements and liquid components. Cultivation is carried out in an aerodynamic environment at a temperature of 28 ° C and a temperature of 6.5-7.0. Biomass, if necessary, concentrate and - 17 - There is a separation process and is disposed of with the help of a freezer and a non-vacuum drying. The resulting dry mass is

-and "lives 4 ^x 10 to 10 days of cells ^" per gram (humidity up to 10),

CONSTRUCTION OF MEDIA FOR JOINT CULTIVATION OF PRODUCTS

5 flax below, g / l:

ΝΗ ^ Cϊ 10.0

Κ ^ ΗΡΟ ^ 1

ΝaΗ ^ ΡΟ ^ 0.5

CaCϊ ^ 0.01

10 ΜβСϊ ^ 0,1

ΟеЗΟ ^ 0.01

ΜηδΟ ^ 0.01 πparaffin (with installation

“Parex”, Law 38.101856-84) 30.0.

15 1 gram of dry biomass, prepared as described above, mixed with a mixture of mineral salts (ΔΑΦ and ΑС) in combination with biomass.-ΔΑΦ: ΑС = 1: 200. The resulting mixture is a purely light-colored product.

The following are examples of liquid preparation methods.

20 biological preparations of various concentrations.

1) For the preparation of a liquid form of a preparation of 1 dry biomass, it is dissolved in 50 ml of warm water (-30 5-30 ^β C), for which 250 mg of Α Α и is used and is used. Then the product is brought up to 10 liters of warm water (ϊ5-30 ^β C), add

25 2.5 - 3.0 g ΑС and stir for a long time. The resulting product contains no less than live cells per ml and 0.27 - 0.32% mineral salt.

2) For the preparation of liquid formulations of a preparation of 50 g of a ready-to-use light-food color, which is a commercially available mixture

30 biomass and mineral salts, grows in 1 liter of warm water and takes in air for 12 hours. the liquid form contains at least 5 x 10 ³ live cells in ml and 5% mineral salt.

3. The use of the basic suspension of the biological product.

35 Beyond the suspension, they take advantage of the biocompositing agent containing mineral salts, which is used for the subsequent dilution. The degree of dilution differs depending on the level of soil contamination, water, and the reserve. - 18 - With a capacity of 100 l, equipped with a stirrer or a device for the air inlet, add 1, 0 kg of the biowater, 250 g of dumplus and 50 g / min. The time for suspension suspension or blowing in air with a temperature of 5 is 12 hours. The concentration of hydrocarbon-oxidizing bacteria is not less than 2χ ϊ 0 ^{, 3} cl. / ml

Then in the same capacity is added 2, 5 - 3, 0 kg diametre of the medium (GUS 19651-74) or any other mineral fertilizers, which includes 100%

4 . Preparation of a working suspension of an ammunition. The convenient suspension of the preparation is prepared without use, for which the basic suspension is diluted with 100 times and is free of charge. 15 5. Soil treatment contaminated with oil and waste products. The working suspension of the preparation is disbanded over the contaminated part from the calculation of 10 liters per one square meter of the cleaned part. Discharging or waiting is carried out with 20 any other purpose intended for these machines and aggregates. A flowing outlet is assembled in a container or in a special recess. After analyzing it for the content of mineral salts and adding to the lack of quantity, the accumulated amount of it is disintegrated over an indifferent study.

Analysis of the suspension for the content of microorganisms is not προ- issued, τ. κ. In the process of destruction of carbohydrates and their num- bers, the main mass of microorganisms is increased and most of them are consumed. 30 Soil processing is preferable to ensure that the soil temperature is at least + 10 ° and not higher than + 40 ° С.

After two - only three weeks, the mineral resources are reduced by means of the inclusion of ΔΑΦ and the United States of America in the event that we disregard the agreement. 35 Expected products are not sold, and soil tests from contaminated sites do not show a decrease in carbohydrate content.

The analysis of the conditions for the supply of hydrocarbons in it is carried out for the processing and then the processing every 10-15 days - 19 - after processing, to achieve the required settlement level. According to the results of the analysis, they calculate the degree of calculation and determine its efficiency.

5 EXAMPLE 2. WATER CALCULATION.

The product, in accordance with the invention, may be used for the processing of natural resources, chemicals and aquacultures.

The working suspension of the product is not available for use 10 times, for which the basic suspension is free of charge by 10 times and is free of charge.

The working suspension of the preparation is applied to the water inlet from the calculation of 1 liter per 1 quarter. Much better. The application is carried out by means of a stand-by with any other purpose, 15 started for these machines and aggregates. It is desirable to carry out the process of purifying water at a temperature of at least + 10 ° С.

Example 3. RESULTS ACCOUNT.

20 The working suspension of the product is prepared for the immediate use; it is, for which the basic suspension is diluted with water at 10 times. χοροшо mixes. The cleaned reservoir fills with a working suspension of the preparation before the level or a little higher than the level of pollution of the wall of the reservoir.

25 It is preferable to handle the liquid at a temperature not lower than + 10 ° С and not higher than + 40 ° С. When calculating the flow rate, it is necessary to stir the suspension or its aeration.

30 Example 4. The use of a biological product for the calculation of populated ecosystems. The commercial calculation of the settled systems is carried out in accordance with the aforementioned methods.

The increase in the activity of the biological product in the process of biodegradation of 35 hydrocarbons of the oil is carried out in the process of the use of the dissolution of the minerals.

Testing method: researching the product in static mode in the absence of mass transfer - 20 - between the urban and the external phase, as well as in the dynamic mode with the transfer of the phase.

Test 1. Test the biological product in the static mode. 5

For the study of the activity of the product in the process of biodegradation of steam carbohydrates, the normal amount of food in the product is small and the amount of food is consumed. 10 In addition, I suggest that the activity of associations with respect to wares significantly increases its activity in relation to carnity.

Tests of domestic appliances in the absence of displacement and aeration. 15 organic substrates in the amount of 2.5 s. interfere with

400 ml of aqueous medium with a different degree of mineralization and mix with 25 ml of biomass. Explore 4 samples with a content of Vas, at 0, 10, 50 and 100 g / l. Κοnτροl sτe- πeni biορazlοzheniya οbρaztsοv (πο οτnοsheniyu κ eτalοnnοmu οb- 20 ρaztsu not sοdeρzhaschemu biοmassu) προvοdyaτ πο οτnοsnτelnοmu sοdeρzhaniyu aροmaτichesκοgο κοmποnen ^-τ 'and subsτρaτe meτοdοm ulτρaφiοleτοvοn sπeκτροsκοπii on πρ. ο----7 Η5 (Β ша ша η η) The results of the market research duplicate with the use of gas-liquid process on the unit of 4500 and 25 with an additional in- texper The data collection obtained by the indicated methods does not exceed 5%.

Method 2. Test the biological product in a dynamic mode.

thirty

Β As a part of the substrate, they use a highly vaporized petroleum product, as a medium - with a content of 0.1% to 10 mass. Biodegradation is carried out in a temperature-controlled glass tank with a volume of 0.5 l, with an average of 35 laden for mechanical pumping (frequency of 60 min.). Researches are carried out at the temperature of 28 ^th with the mass ratio of "oil: mineralized environment: biomass" (1: 99: 0,0001). If necessary, take 20 seconds to remove it. Analysis of the method of gas-liquid hromato- - 21 - changes on Ρ иб--00--4500 00 change in the concentration of η-parameters C, -C ₃₀ .

TEST RESULTS.

Method 1. The biodegradation data for model hydrocarbon mixtures are shown in Table 2.

Table 2.

10

The degree of biodegradation of the hexadecane in the initial concentration.

5 days 1 2 3 6 12

Community

(% ΝаСϊ)

0 2.2 5.7 .6.8 16.3 31.4

0 1 13.4 .19.2 24.4 41.5 67.3

5 13.5 17.0 21.1 35.3 59.4

10 9.0 11.7 15.4 24.4 37.1

25 As can be seen from the table, the maximum degree of biodegradation is reached in the interval of the growth area from 1 to 5%.

Method 2. The data for the biological sample supplied are shown in Table 3.

thirty

The results obtained are related to the fact that the association of microorganisms actively disrupts the saturated hydrocarbons of the product in the range of 10 min mineralizacin. % The optimal for the process of biodegradation of steamed 35 carbohydrates is the percentage of the total cost of production, up to 50%, the increase in consumption is 100%. - 22 -

Table 3.

Dependence of the degree of biodegradation of steam carbohydrates from the crude oil due to the concentration of medium 5 and the time of exposure

SuΤΚI 3 5 10 15 20

Community

10 (% Νасϊ)

0.1 5 7 7 8 10

1 50 80 90 95 95

3 55 80 87 94 100

5 50 80 88 96 100

15 10 15 45 60 75 80

The presented data shows a high efficiency of the biological product in accordance with the invention, the difference in product quality is different.

20

Intended use.

The present invention may be used on a large scale for the purpose of calculating and restoring 25 non-essential goods, including

Claims

- 23 -

ΦΟΡΜУΛΑ ΑΟΟΡΕΤΕΗΡΕΤΕΗ

5 1. A biological product for the calculation of water and soil is free from contamination with oil and non-food products, including a mixture of carbohydrate-related strains ΒΚΜ-ΑС-1500Ο, ΚЬάάάсοссиδδδ шаг шаг шагχ χδΒΚΜ ΒΚΜ-ΑС-150 150, ΚΚάάссοссδδδδδδег егΙгггορρρδδδδ

10 ΒΚΜ-ΑС-1502Ο, Ρδeiάοшοηаδ δϊиϊζегχ ΒΚΜ-Β-19720, Саηάχάа 5р. ΒΚΜ-Υ-2778ϋ.

2. The biological product is π .1, which is different from the fact that it is a natural association of microorganisms that is isolated from non-casualties.

15 3. Biological preparation π 1, which is different from the fact that it is a mixture of individual strains of microorganisms, which are obtained by mixing them.

4. The biological product is π .1, which is distinguished by the fact that it is 20, which is a unique mixture of individual strains of microorganisms cultivated in the process of cultivation.

5. Biological preparation πο ππ. v, h, 4, distinguished by the fact that the indicated strains of microorganisms in the mixture are contained in the ratio (0-1): (0-1): (0-1): (0-1): (0-1), Correspondingly.

25 6. Sποsοb οchisτκi vοdy and ποchvy οτ zagρyazneniya neφτyu and neφτeπροduκτami, vκlyuchayushy οbρabοτκu vοdy and / or ποchvy biοπρe- πaρaτοm, sοdeρzhashim mixture uglevοdοροdοοκislyayushiχ miκροορganiz- mοv in πρisuτsτvii mineρalnyχ sοley in uslοviyaχ aeρatsii, οτli- τem aspirants, chτο isποlzuyuτ biοπρeπaρaτ, sοdeρzhashy strains

30 microorganisms ΚЬ. 5 p. ΒΚΜ-ΑС-1500Ο, ΚЬ. SHEGΧ5 ΒΚΜ-ΑС-150Ш, κЬ.егуΙЬгοροϋδ ΒΚΜ-ΑС-1502Ρ, Ρδ.δϊиϊζегχ ΒΚΜ-Β-19720 and Саηάχάа 5р. ΒΚΜ-Υ-2778ϋ.

7. The method of operation, which is characterized by the fact that the biological product is used in quantities that include at least 2 years of business

35 current micro cells per 1 sq. M. contaminated foreign countries.

8. A method of π. 6 or 7, which is characterized by the fact that it is possible to wait for the suspension of a biological product in the process of supply of a small amount of food, which is inadequate to the environment.

- 24 -

9. A method, one of which comes from 6 to 8, which is different from the fact that it is supplied with a suspension that contains biological and mineral salts, to improve the rate of warming

5 collect and use direct or large quantities, adding, if necessary, nitrogen and / or discharged salt.

10. The case is π.9, which differs from the fact that the size and frequency of the disposal of the soil, which is soiled in a foreign soil, depends on the degree of pollution of the soil with carbohydrates.

You are welcome to one of ππ. On the other hand, there are six, which means that they handle water and / or soil with an increase of up to 10%.

12. The use of a biological product containing strains of microorganism. 5ρ. ΒΚΜ-ΑС-1500Э, ЬЬ. YuEGΧ5 ΒΚΜ-ΑС-150Ш,

15 b.egu £ bgrp1x5 ΒΚΜ-ΑС-1502ϋ, Ρδ.δΙиΙζегχ ΒΚΜ-Β-19720 And Caηάχάa 5р. ΒΚΜ-Υ-2778ϋ for cleanly populated soil and soil systems with pollution from oil and non-oil products.

13. The use of a biological product containing the strains of the micro-20 of the Ganism ΒΚΜ-ΑС-1500Ο, ΚЬ. stepχ: ΒΚΜ-ΑС-150Ш,

ЕгЬ.егуΙЬгοροϋδ ΒΚΜ-ΑС-1502Э, Ρδ.δΙлιΙζегχ ΒΚΜ-Β-19720 and Саηάχάа 5р. ΒΚΜ-Υ-27780 for a clearing house.