GB1059886A - Improvements in or relating to the production of micro-organisms - Google Patents

Improvements in or relating to the production of micro-organisms

Info

Publication number
GB1059886A
GB1059886A GB49057/62A GB4905762A GB1059886A GB 1059886 A GB1059886 A GB 1059886A GB 49057/62 A GB49057/62 A GB 49057/62A GB 4905762 A GB4905762 A GB 4905762A GB 1059886 A GB1059886 A GB 1059886A
Authority
GB
United Kingdom
Prior art keywords
micro
organism
hydrocarbon
growth
nutrient medium
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB49057/62A
Inventor
Jean Antoine Filosa
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BP PLC
Original Assignee
BP PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BP PLC filed Critical BP PLC
Priority to GB49057/62A priority Critical patent/GB1059886A/en
Priority to CH1271866A priority patent/CH476835A/en
Priority to US330527A priority patent/US3264196A/en
Priority to DK133765AA priority patent/DK108532C/en
Priority to DK133465AA priority patent/DK109222C/en
Priority to DK133565AA priority patent/DK108492C/en
Priority to DK603163AA priority patent/DK117622B/en
Priority to SE14526/63A priority patent/SE325545B/xx
Priority to CS415366*1A priority patent/CS155140B2/cs
Priority to BR155791/63A priority patent/BR6355791D0/en
Priority to DE1963B0074851 priority patent/DE1470507C3/en
Priority to DE1963B0085070 priority patent/DE1442067A1/en
Priority to AT834065A priority patent/AT262201B/en
Priority to DK372166AA priority patent/DK117212B/en
Priority to DK372266AA priority patent/DK120582B/en
Priority to NO00165503A priority patent/NO127540B/no
Publication of GB1059886A publication Critical patent/GB1059886A/en
Priority to SE10256/67A priority patent/SE324344B/xx
Priority to FI672578A priority patent/FI43302C/en
Priority to CY42568A priority patent/CY425A/en
Priority to MY196865A priority patent/MY6800065A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/26—Processes using, or culture media containing, hydrocarbons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/921—Candida
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/921—Candida
    • Y10S435/923—Candida lipolytica
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/911—Microorganisms using fungi
    • Y10S435/944—Torulopsis

Abstract

A micro-organism, particularly yeast, capable of growth in the presence of a hydrocarbon feedstock consisting wholly or in part of straight chain hydrocarbons in a growth stage is cultivated in the presence of the feedstock, an aqueous nutrient medium and a gas containing free oxygen and subsequently, if necessary, the micro-organism is separated from the bulk of the unconsumed hydrocarbon. The content of straight-chain hydrocarbon in the feedstock is thereby reduced with an improvement in the pour point.ALSO:A micro-organism, particularly yeast, capable of growth in the presence of a hydrocarbon feedstock consisting wholly or in part of straight chain hydrocarbons, in a growth stage is cultivated in the presence of the feedstock, an aqueous nutrient medium and a gas containing free oxygen and subsequently, if necessary, the micro-organism is separated from the bulk of the uncomsumed hydrocarbon whereby there is obtained a micro-organism free of a substantial amount of hydrocarbon but containing a small amount of hydrocarbon as contaminant, and thereafter in a purification stage the micro-organism is maintained with an aqueous nutrient medium and a gas containing free oxygen, whereby the hydrocarbon contaminating the micro-organism is reduced in quantity or is eliminated. The growth stage and purification stage may be carried out continuously in separate fermenters or in different zones of the same fermenter. After the growth stage, the major part of the continuous aqueous phase is first separated by decanting and centrifuging. About 80% of the aqueous phase recovered is returned to the fermenter together with make-up quantities of the necessary nutrients. The micro-organism "cream" also obtained by the separation is then mixed with an aqeuous nutrient medium, which may be the same as or different from the aqueous nutrient medium employed in the growth stage, and is maintained in admixture with a gas containing free oxygen whereby the hydrocarbon contaminating the micro-organism is reduced in quantity or is eliminated. Again, the major part of the continuous aqueous phase is separated with recycle of a proportion of the recovered aqueous phase either to the growth stage or to the purification stage. The micro-organism "cream" obtained by this second separation may be further treated for the removal of traces of oil held to the cells, as by washing with an aqueous solution of a surfactant and thereafter with water. Examples are directed to the growth on heavy-gas oil of Candida lipolytica from an inoculum on mixed C9-C16 normal hydrocarbons, and to a subsequent purification of the yeast under the same culture conditions except for the lack of the heavy-gas oil. The purified yeast was recovered by centrifuging and washing at +5 DEG C, and was then freeze-dried. The dried yeast exhibited a substantially reduced lipid content.
GB49057/62A 1962-12-31 1962-12-31 Improvements in or relating to the production of micro-organisms Expired GB1059886A (en)

Priority Applications (20)

Application Number Priority Date Filing Date Title
GB49057/62A GB1059886A (en) 1962-12-31 1962-12-31 Improvements in or relating to the production of micro-organisms
CH1271866A CH476835A (en) 1962-12-31 1963-12-12 Process for obtaining microorganisms with the simultaneous elimination, at least in part, of straight chain paraffinic hydrocarbons from the hydrocarbon mixtures in which they are contained
US330527A US3264196A (en) 1962-12-31 1963-12-16 Purification of micro-organisms cultivated on hydrocarbon feedstock
DK133765AA DK108532C (en) 1962-12-31 1963-12-23 Process for removing normal paraffins and olefins having at least 10 carbon atoms from hydrocarbon oils.
DK133465AA DK109222C (en) 1962-12-31 1963-12-23 Process for the complete or partial removal of normal paraffins and olefins having at least 10 carbon atoms from hydrocarbon mixtures.
DK133565AA DK108492C (en) 1962-12-31 1963-12-23 Process for the preparation of a yeast product for use as feed.
DK603163AA DK117622B (en) 1962-12-31 1963-12-23 Process for the preparation of a microorganism-containing product for use as feed.
SE14526/63A SE325545B (en) 1962-12-31 1963-12-28
BR155791/63A BR6355791D0 (en) 1962-12-31 1963-12-30 PERFECT PROCESS FOR THE PRODUCTION OF MICRO-ORGANISMS IN A CULTURE MEDIA CONTAINING LINEAR CHAIN HYDROCARBONS
CS415366*1A CS155140B2 (en) 1962-12-31 1963-12-30
DE1963B0074851 DE1470507C3 (en) 1962-12-31 1963-12-30 Cultivation of microorganisms
DE1963B0085070 DE1442067A1 (en) 1962-12-31 1963-12-30 Process for the production of proteinaceous material
AT834065A AT262201B (en) 1962-12-31 1963-12-31 Process for obtaining a mass of microorganisms
DK372166AA DK117212B (en) 1962-12-31 1966-07-18 Process for the preparation of a microorganism-containing product for use as feed.
DK372266AA DK120582B (en) 1962-12-31 1966-07-18 Process for the preparation of a microorganism-containing product for use as feed.
NO00165503A NO127540B (en) 1962-12-31 1966-11-08
SE10256/67A SE324344B (en) 1962-12-31 1967-06-30
FI672578A FI43302C (en) 1962-12-31 1967-09-27 Method for preparing food yeast
CY42568A CY425A (en) 1962-12-31 1968-01-08 Improvements in or relating to the production of micro-organisms
MY196865A MY6800065A (en) 1962-12-31 1968-12-31 Improvements in or relating to the production of micro-organisms

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB49057/62A GB1059886A (en) 1962-12-31 1962-12-31 Improvements in or relating to the production of micro-organisms

Publications (1)

Publication Number Publication Date
GB1059886A true GB1059886A (en) 1967-02-22

Family

ID=10450956

Family Applications (1)

Application Number Title Priority Date Filing Date
GB49057/62A Expired GB1059886A (en) 1962-12-31 1962-12-31 Improvements in or relating to the production of micro-organisms

Country Status (11)

Country Link
US (1) US3264196A (en)
AT (1) AT262201B (en)
BR (1) BR6355791D0 (en)
CS (1) CS155140B2 (en)
CY (1) CY425A (en)
DK (1) DK108492C (en)
FI (1) FI43302C (en)
GB (1) GB1059886A (en)
MY (1) MY6800065A (en)
NO (1) NO127540B (en)
SE (1) SE324344B (en)

Families Citing this family (9)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3427223A (en) * 1964-06-10 1969-02-11 Exxon Research Engineering Co Coagulating microbial cells to enhance their separation
US3508927A (en) * 1965-09-15 1970-04-28 Exxon Research Engineering Co Use of unsaturated organic acids as bacterial growth promoters
GB1141107A (en) * 1966-01-26 1969-01-29 Kyowa Hakko Kogyo Company Ltd Process for producing sugars by fermentation
GB1168834A (en) * 1966-05-13 1969-10-29 British Petroleum Co Improvements in or relating to the production of Micro-Organisms
GB1168835A (en) * 1966-05-13 1969-10-29 British Petroleum Co Improvements in or relating to the production of Micro-Organisms
US3489648A (en) * 1966-12-22 1970-01-13 Phillips Petroleum Co Microbial hydrocarbon consumption
US3622465A (en) * 1967-03-10 1971-11-23 Allied Chem Protein from normal hydrocarbons
US3620927A (en) * 1969-06-26 1971-11-16 Gulf Research Development Co Cultivation of micro-organisms on hydrocarbons
US3904476A (en) * 1973-05-29 1975-09-09 Mobil Oil Corp Treatment of cells of a hydrocarbon-consuming microorganism

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2697061A (en) * 1950-08-17 1954-12-14 Texaco Development Corp Processing of hydrocarbons
US2697062A (en) * 1951-03-30 1954-12-14 Texaco Development Corp Processing of hydrocarbons
US2742398A (en) * 1951-06-09 1956-04-17 Texaco Development Corp Method of removing deposits of wax and like materials
US2982692A (en) * 1957-06-26 1961-05-02 Hardin B Mcdill Dewaxing of oils
US3069325A (en) * 1959-12-21 1962-12-18 Phillips Petroleum Co Treatment of hydrocarbons

Also Published As

Publication number Publication date
FI43302C (en) 1971-03-10
US3264196A (en) 1966-08-02
DK108492C (en) 1967-12-27
FI43302B (en) 1970-11-30
AT262201B (en) 1968-06-10
NO127540B (en) 1973-07-09
CY425A (en) 1968-01-08
BR6355791D0 (en) 1973-08-28
CS155140B2 (en) 1974-05-30
SE324344B (en) 1970-06-01
MY6800065A (en) 1968-12-31

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