GB1461408A - Fermentation process for the production of lipase - Google Patents

Fermentation process for the production of lipase

Info

Publication number
GB1461408A
GB1461408A GB2513273A GB2513273A GB1461408A GB 1461408 A GB1461408 A GB 1461408A GB 2513273 A GB2513273 A GB 2513273A GB 2513273 A GB2513273 A GB 2513273A GB 1461408 A GB1461408 A GB 1461408A
Authority
GB
United Kingdom
Prior art keywords
lipase
broth
microbial lipid
lipid
weight
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
GB2513273A
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BP PLC
Original Assignee
BP PLC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BP PLC filed Critical BP PLC
Priority to GB2513273A priority Critical patent/GB1461408A/en
Priority to DK279974A priority patent/DK136255B/en
Priority to JP5867874A priority patent/JPS5031088A/ja
Priority to FR7418137A priority patent/FR2230724B1/fr
Publication of GB1461408A publication Critical patent/GB1461408A/en
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase

Abstract

1461408 Lipase BRITISH PETROLEUM CO Ltd 10 May 1974 [25 May 1973] 25132/73 Heading C3H A fermentation process for the production of lipase involves cultivating a lipase-producing micro-organism in a broth comprising a microbial lipid as the carbon source typically in a proportion of 1 to 25 g./l., an aqueous nutrient medium and a gas containing free oxygen and thereafter recovering a broth fraction containing lipase. Lipase in solid form may be isolated from the broth by employing conventional techniques. Lipase-producting micro-organisms: species of bacteria, yeast and fungi (specified) but especially Candida lipolytica CBS No. 6331. The microbial lipid is preferably that obtained by extracting with isopropanol the biomass produced by the cultivation of the hydrocarbonutilizing yeast Candida tropicalis CBS No. 6373 on a gas oil boiling range hydrocarbon as the carbon substrate. Phospholipids are removed from the microbial lipid by solvent treatment before use. The yield of lipase is enhanced by including in the broth (1) a dispersant for the lipid, e.g. 0À3-3À0 parts by weight per part by weight of the microbial lipid, of a petroleum fraction which contains n-alkanes and has a boiling range of 250-380‹ C., and (2) non-ionic surface active agent, e.g. 0À05-1À0 part by weight (based on microbial lipid) of either a fatty acid ester of a polyalkylene oxide sorbitol condensate or sorbitan mono fatty acid ester. The fermentation is carried out under mild conditions of agitation with aeration rates in the range 30 to 60 vol./vol./hr. An antifoaming agent (specified) may be included in the broth.
GB2513273A 1973-05-25 1973-05-25 Fermentation process for the production of lipase Expired GB1461408A (en)

Priority Applications (4)

Application Number Priority Date Filing Date Title
GB2513273A GB1461408A (en) 1973-05-25 1973-05-25 Fermentation process for the production of lipase
DK279974A DK136255B (en) 1973-05-25 1974-05-22 Process for the production of lipase.
JP5867874A JPS5031088A (en) 1973-05-25 1974-05-24
FR7418137A FR2230724B1 (en) 1973-05-25 1974-05-24

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
GB2513273A GB1461408A (en) 1973-05-25 1973-05-25 Fermentation process for the production of lipase

Publications (1)

Publication Number Publication Date
GB1461408A true GB1461408A (en) 1977-01-13

Family

ID=10222725

Family Applications (1)

Application Number Title Priority Date Filing Date
GB2513273A Expired GB1461408A (en) 1973-05-25 1973-05-25 Fermentation process for the production of lipase

Country Status (4)

Country Link
JP (1) JPS5031088A (en)
DK (1) DK136255B (en)
FR (1) FR2230724B1 (en)
GB (1) GB1461408A (en)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2147004A (en) * 1983-08-02 1985-05-01 Kanegafuchi Chemical Ind Interesterification of fats
WO1988002775A1 (en) * 1986-10-17 1988-04-21 Novo Industri A/S Positionally non-specific lipase from candida sp, a method for producing it, its use and a recombinant dna process for producing it
US5273898A (en) * 1986-10-17 1993-12-28 Noro Nordisk A/S Thermally stable and positionally non-specific lipase isolated from Candida

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS6030680A (en) * 1983-07-30 1985-02-16 Amano Pharmaceut Co Ltd Method for imparting esterase activity to lipase
JP6362992B2 (en) 2014-10-20 2018-07-25 三菱日立パワーシステムズ株式会社 Heat exchanger monitoring device and heat exchanger monitoring method

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB2147004A (en) * 1983-08-02 1985-05-01 Kanegafuchi Chemical Ind Interesterification of fats
WO1988002775A1 (en) * 1986-10-17 1988-04-21 Novo Industri A/S Positionally non-specific lipase from candida sp, a method for producing it, its use and a recombinant dna process for producing it
US5273898A (en) * 1986-10-17 1993-12-28 Noro Nordisk A/S Thermally stable and positionally non-specific lipase isolated from Candida

Also Published As

Publication number Publication date
DK279974A (en) 1975-01-20
DK136255B (en) 1977-09-12
DK136255C (en) 1978-02-13
FR2230724A1 (en) 1974-12-20
FR2230724B1 (en) 1977-10-14
JPS5031088A (en) 1975-03-27

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Legal Events

Date Code Title Description
PS Patent sealed
PCNP Patent ceased through non-payment of renewal fee