SU1071637A1 - Strain of black yeast exophiala nigrum r-11 for use in purification of waste liquors from phenols and lignin - Google Patents

Abstract

The stem of the black yeast Echopharar nigrum R-11 (deposited in the Central Museum of Promoter Microorganisms of the Institute of Scientific Research Institute of Energetics, collection number TSMPMu-309) used to purify wastewater from phenols and lignin.

Description

 The invention relates to microbial logic and can be used for the biological treatment of wastewater from organic compounds, in particular from phenolic and sulfur-containing compounds, in pulp and paper, petrochemical, coal chemical and other industrial fields. A known strain of Citrobacterfgeundii 21 for sewage treatment from dodecyl sulfates. A known strain Pseudomonas sp 1 for the purification of industrial wastewater from alkyl sulphates til. The method of purification of wastewater from phenols is also known. Fungi C21 The disadvantages of these strains are the low activity of destruction with respect to phenolic compounds. The aim of the invention is a strain that is capable of utilizing phenolic and lignin. The goal is achieved by using a black yeast strain of the species Exophiala nigrum R-11 which performs biological purification from phenols and lignin. Culture; black yeast identified as E nigrum J tvapelen from samples of Baikal water taken in the coastal zone of Southern Baikal using membrane filters. Strain R-11 was isolated by cloning using a micromine pushor and subsequent sieving. About forty clones were obtained, serving as material for selection and toxicogenetic testing. Shtam14 black yeast Exophiala nigrum R-11 is stored in the collection of the genetics laboratory of the Research Institute of Biology of the Irkutsk State University and in the collection of the All-Russian Scientific Research Institute of Genetics of Industrial Microorganisms (Moscow), where it has been assigned the registration number CMPMU-309. After testing for pathogenicity for warm-blooded animals and humans, as well as for cereal crops, strain R-11 is recommended for use in industry as non-pathogenic. G1tamm black yeast - Exophiala nigrum R-11 has the following features. Morphological signs. In liquid media, without shaking, the strain forms oval-elongated cells 5.2 / 8.3x1.9-4.7 microns. Reproduction by apical or lateral budding, often unilateral. And rounded large cells form mycelium from the hyphae. Mycelium 30-dayP. cultures are strongly developed, septate, irregularly branched, mostly true, substrate or air. Cultural features. With growth in a liquid glucose-peptone medium or on wort for 3 days, turbidity is observed, at the bottom of the sediment, not a sleep film, is absent or partial. After a month, the ring is very thick, there are isolated mycelium on the surface of the medium. At the bottom of a slight sediment of yeast-like cells, no turbidity. On agar media, the stroke is straight, the profile is convex, the edge is smooth, the color is black glistening, consistency is weakness. After a month, the stroke is black or brownish-gray, unevenly lowered, the surface is folded, the edge is villous-edged. The giant colony reaches a diameter of 19-25 mm, is convex, lowered, brownish-sulfur with a smooth edge, with a high central part surrounded by a smooth black roller, rare radial folds. Physiological signs. Laerob, forms a black pigment, sugar does not ferment. Well assimilates D. Glucose, D-xylose, sucrose, soluble starch, glycerin, D-lactic acid, slowly assimilates ot-rhamnose, α -arabinsu, maltose, D-ijiaHHT, D-sorbitol, Poorly assimilates D-galactose, , ethanol, dulcite, i-inositol, does not assimilate, lactose, citric acid, hexadecan. From nitrogen sources. Nitrite is assimilated, growth is slow in a medium without vitamins, does not grow on a medium with 50% glucose, and maintains a concentration of Had equal to 7%. The optimum growth temperature is 20-25 ° C :, does not increase at 30-37 ° C. It is sensitive to 100 µm / ml cycloheximide, has urease activity, does not contain acid on chalky agar, gelatin does not thin, does not hydrolyze fat, does not form ether and starch-like compounds. Shta№1 R-11 is able to dispose of phenols. Example 1. The strain of black yeast Expplviala nigrum R-11 is grown on wort agar or. the glucose peptone medium is washed, 100 ml of water containing pyrocatechin or any other phenol: hydroquinone, phenol, gwa cola at a concentration of 10 m are added to a 100 ml water solution, 2 g of black yeast is suspended in a suspension and after 24 h the residual phenol content is determined by potentiometric titration. The degree of purification from pyrocatechol is 96.6%, from hydroquinone - 86.2%, from phenol - 04.3%, gva cola - 82.3%.

Strain. R-ll is capable of assimilating lignin as a carbohydrate source.

Example 2. The data are given in table. one.

Strain R-11 is capable of growing in environments where dioxane-lignin is the only source of carbohydrate nutrition.

P RIM e p 3. The growth rate of the strain R-11 on a 1% solution of dioxane-lignin is shown in table. 2

After 20 days of cultivation, the concentration of cells in 1 ml of nutrient dioxane lignin media increases by 9, U compared to baseline. For 5 days, strain P-11 absorbs 30% of the lignin dissolved in the neutral medium.

The high resistance of the strain R-11 to organic and inorganic compounds and their mixtures was found, ranging from 1–1 ms to the sites of sulphate-cellulose production.

Indicators

Example 4. In table. 3 shows the survival rate of strain R-11 under the action of high concentrations of compounds and mixtures.

The resulting strain allows the treatment of sulphate-cellulose effluents and can be re-recommended as a component of microbiocenoses, performing biological purification from phenols and lignin, does not cause secondary contamination.

I .T a b l. and c a 1

15

WER

59

100

Nitrogen

0 base + lignin (0.5%)

84142.4

Table 2

Cultivation time

days

Cell concentration in 1 ml

- (x 10) 6.53 35.1 52V4

Growth rate

Concentration, mg / l

Compound (mixture)

A mixture of monophenols (80% gua cola, 9.5% o-cresol, 9.5%. Vanillin, 1% phenol)

A mixture of diphenols 50% hydroquinone, 25% pyrocatechin, 25% resorcinol) A mixture of mono-diphenols (50% mixture of monophenols, 50% mixture of diphenols)

64.5

53.2

9.9

8.1

8.0

5.4

Table 3

Survival tx f m),%

93,1i- 9,7

87.0 ± 9.7 81.4 ± 4.0

Claims (2)

STRAIN OF BLACK YEAST EX0RN1ADA Igsky K-11, USED for purification of wastewater from phenols and lignin. (57) Stamp of black yeast Ехρΐιϊαίότ phegish K-11 (deposited at the Central Museum of Industrial Microorganisms of the Institute "VNIIgenetika", collection number: TsMPMU-309), used for wastewater treatment from phenols and lignin. / 1 1071637 2