SU962304A1 - Strain pseudomonas rathonis for use in industrial effluents from volgonate - Google Patents

Description

The invention relates to microbiological industry and relates to a new bacterial strain used for the treatment of industrial wastewater from volgonate-based detergents (alkyl sulfonate), for example, in household chemicals plants, chemical plants for the production of surface active substances.

1 Known stratigll .Citrobacterfirumdii 2Т and Pseudomonas er. 1C, destructive anionic surfactants — alkyl sulfates 1 and 2J.

However, the known strands are not destructors of the volgonate.

The purpose of the invention is to obtain an active volgonate destructor.

The strain Pseudomonas rathonis T has the following morphological and physiological characteristics.

Morphological features: cells are small, rod-shaped, arranged singly or in pairs, gram-negative, mobile with polar flagellation. Colonies are round, smooth, shiny, slightly convex, yellowish in color. At the BCH, the strain forms a uniform moderate thread and a weak film, after a week - a dense precipitate.

Physiological and biochemical characteristics: the culture is oxidatively positive, gelatin does not thin, does not possess lecithinase and levansacharazny activity, arginine is anaerobically decomposed; forms a servo and does not produce an indole; Milk does not change, glu, goat does not ferment. On the mineral environment of Omele Nsky with water-soluble

10 indicator bromthymol blue forms acids from glucose, maltose and glycerin and does not use lactose, sucrose, arabinose, rhamnose, xylose, inositol, mannitol, salicin, dulcitol,

15 sorbitol, sodium tartrate. The culture assimilates sodium lactate, acetate, and citrate.

 receive as follows.

Claims (2)

In order to obtain an active destructive soil, the soil taken on the territory of the plant is introduced into flasks with a liquid nutrient medium of the following composition, g: i; , i; KCl 0.5; MgCli0, 1 and 400 mg / l of volgonate as the 25 only source of carbon and energy and cultured for 45 days on a rocking chair at 28-30 fC. The ability of microorganisms to destroy the studied compound is judged by its 30 | ubd in a liquid medium. The obtained culture is scattered on MPA and pure cultures of bacteria are isolated, the destructive activity of which is again tested in a liquid synthetic medium. A colony capable of destroying the volgonat of a given concentration is screened onto oblique agar and this strain is obtained. The strain is stored in the collection of ICCMC of the Academy of Sciences of the Ukrainian SSR 35 and deposited at the VNIIg netika, collection number ShchPM B-2330. PRI me R. To the laboratory installation, an aerofilter-aerotank, representing a column consisting of a biofilter with a load of pieces of refractory brick and aerotank placed with it, for 7-10 days. A suspension of the strain-destructor is supplied to build up the biofilm on the batch. Ke. After that, a synthetic stock of the composition is supplied to the upper part of the installation using a peristaltic pump Reg1rshpr, g / l: 1, 1) ko; 0.5; MgCl-j. 0,1} volgonat 1; distilled water 1l. The amount of liquid 3 l / day. aeration 1.5-2.0 l of air / min, aerotank volume 9.5 l. After downloading, the installation works for 2 months. In the purified liquid, the volgonate was not detected. As follows from the example, the stalk destroys the volgonat in high concentration (1 g / l). The use of this strain makes it possible to release industrial wastewater from volgonate in local plants without first diluting these waters, which reduces the cost of purification and prevents disruption of the operation of activated sludge in biological purification facilities, which are observed when the highly volatile waste is infused into them. The release of industrial wastewater from volgonat is important for the purification and protection from pollution of natural water bodies and drinking water sources. . Claims of the invention: Psendomonas rathonis T strain (deposited at the Central Museum of Industrial Microorganisms of the Institute of Scientific Research Institute of Genetics, collection number ShchPM B-2330) used to purify npoNuffimeHHHx wastewater from volonate. Sources of information, taken into account during examination 1, USSR Author's Certificate No. 657063, cl. C 12 N 15/00, 1977. 2. USSR author's certificate number 560906, cl. C 02 F 3/34, 1975.