RU1794891C - Method of biological treatment of geothermal and accompanying oil waters from phenols - Google Patents

Abstract

Usage: biological purification of geothermal and associated water from phenols and purification of phenol-containing wastewater in the oil, gas and other industries. Essence: wastewater is treated in an aeration tank with immobilized adapted microorganisms. As a seed, an association of adapted thermophilic bacteria p.Aftho bacter and p.BneNlus is introduced in an amount of 2-5 g / l (dry biomass). The purification process is carried out at 20-60 ° C. 2 tab.

Description

The invention relates to methods for microbiological purification of geothermal and associated petroleum water from phenols and can be used for purification of phenol-containing wastewater in oil, gas and other industries.

Known methods for the biological treatment of industrial wastewater from phenols, surfactants. heavy metals and other harmful substances, for which it is recommended to use both aquatic microorganisms, which are part of a variety of silt cenoses, biofilms, and adapted highly active microorganisms.

A known method for the biological treatment of concentrated wastewater from organic compounds, comprising treating activated sludge in an aeration tank in the presence of Mycobacterlum lacticolum strain in an amount of 1.5-2% of the dry weight of activated sludge.

The disadvantage is that bacteria (mesophiles) are used that are active in a narrow temperature range of 18-35 ° C. An increase in the temperature of the medium to 38–40 ° C leads to inhibition of the biochemical activity of microorganisms, which cannot be restored for a long time.

Closest to the proposed method is a method that uses an inert carrier in the aeration tank (basalt fiber, fiberglass, glass, weight 5-10 g / l) and adapted microorganisms from the source of wastewater.

However, the selected prototype, having a high purification effect, has a number of significant drawbacks: the method is carried out with preliminary treatment of wastewater with inorganic reagents in a certain proportion; biological treatment is carried out by adapted mesophilic bacteria that are active in UzV |

2

00 h

com temperature range (18-30 ° C); The commissioning period of the installation is 7-14 days.

The purpose of the invention is to shorten the commissioning period and increase the efficiency of purification of geothermal and associated oil waters from phenols;

The method is carried out as follows /; ....- ::,:: ;;: ;;,.:: ....-: ..-

Geothermal or associated petroleum waters with a salinity of 10-100 g / l, a temperature of 20-60 ° C and a phenol content of 1 -20 mg / l are fed for cleaning to an aeration tank with an inert carrier filled with glass (ruffs). The air in the aeration tank is supplied continuously by a compressor in an amount of 20-30 l / h. Cleaning is carried out in the temperature range

2 ° C :. -::.. / ;; :.; V;:;; .v; -:; :: :: ::;: g :.

In order to shorten the commissioning period and increase the efficiency of purification of phenol-containing or associated oil waters, an aeration tank is introduced with 2-5, g / l (dry biomass) association of thermophilic bacteria with high destructive activity to phenol. -: - v.;.-..., ..; :: -. . h. -. .., .-. At. . .. ....... .. The association of microorganisms is selected from the Nakopitel - glassworm of the source wastewater and consists of thermophilic bacteria p.Arthobacter and

p. BacHhis. ::: .-;, - ;; /; ..., ......- :. ..- ,, To immobilize bacteria on glass-cutters. during the first three days, the installation operates in the recirculation mode, while the environmental parameters such as pH, optical density, COD, phenols are also monitored. The results of the biological treatment of geothermal waters (Ternair) at various values of the introduced biomass of bacteria — seed and temperature and the data of the prototype are presented in Table 1.

As follows from the data table. 1, a high cleaning efficiency is achieved by introducing a seed in an amount of 2-5 g / l (dry biomass) in the temperature range of 20-60 ° C. A further increase in biomass, for example, up to 6 g / l, does not contribute to an improvement in the purification of geothermal waters, 4fo is apparently associated with physiological

features of the association of bacteria in the stationary phase of growth.

Significant differences of the proposed method are: the use as selection seed of an association of selected thermophilic bacteria p. Arthobacter and p. Baclllus in an amount of 2-5 g / l (dry biomass); achievement of the effect of purification from phenol at a temperature

0 20-60 ° C.

Geothermal water with a salinity of 24.2 g / l, a phenol concentration of 7.8 mg / l and a temperature of 45-55 ° C (SKV.28-T Ternair, DASSR) is subjected to purification. The purification of geothermal water from phenols is carried out in a laboratory aeration tank of 2 l volume, filled with an inert load - glass cutters weighing 15 g / l at a temperature of 45RS. An association with the thermophilic bacteria P. afthobacter and p, Bacillus, selected from the source wastewater, was introduced into the aeration tank.

Before introduction into the aeration tank, microorganisms are grown on a solid feeder 5 medium (m fish-peptone agar) in Petri dishes for 24 hours at a temperature of 37 ° С. The biomass from the Petri dishes is washed off with sterile tap water, centrifuged and introduced into the aeration tank in an amount

0 5 g / l (dry biomass).

Immobilization of microorganisms is carried out by direct contact of bacterial cells with fiber loading for three days of operation of the aeration tank in recirculation mode. Further, the aeration tank is transferred from recirculation to flow mode at a rate of 1 volume of liquid per day (dilution degree D 0,).

The results of the treatment of geothermal waste waters on the third day of operation of the aeration tank according to the main indicators, in comparison with the prototype, are presented in Table 2. The efficiency of purification from phenols is 100%.

5 According to sanitary-hygienic indicators, purified geothermal water can be used as technical for industrial needs, or it can be discharged into a reservoir.

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Formula and zobretten.

A method for biological purification of geothermal and associated petroleum water from phenols, comprising introducing into the wastewater a culture of bacteria of the genus Bacillus, characterized in that, in order to shorten the commissioning period and increase the purification efficiency, the bacteria of the Arthobacter and Bacillus genera are used as bacteria immobilized on an inert carrier, the introduction of bacteria into water is carried out in an amount of 2-5 g / l by dry biomass.

Note. The numerator is untreated water; the denominator is purified water.

Table 1

T a 6 l and 2