SU1640155A1 - Strain of bacteria pseudomonas mendocine used for waste water treatment from sodium alkyl benzene sulfonate, syntamide and syntanol - Google Patents

Abstract

The invention relates to technology, in particular to the biodegradation of xenobiotics, and can be used to purify wastewater from IIAB (sulfonol, syntamide, syntanol) formed during the production or use of anionic (AIIAB) and non-ionic (HIIAB) surfactants, and is a new strain possessing destructive properties for toxic xenobiotics - AIAV (sulfonol) and nonionic surfactants (syntamide, syntanol), which are used by him as the sole carbon source at a concentration of 100-500 and 600-1000 mg / dm 3 s responsibly under aerobic conditions and a temperature of 30 ° C, pH 7,0-7,2, addition of salts of nitrogen, phosphorus and potassium in the culture conditions of the flow. After 48 h, the waste water purified in the bioreactor contains 4–15 mg / dm 3 of sulfonic acid and 5–20 mg / dm 3 of nonionic surfactants. 2 tab. in s

Description

The invention relates to technology, in particular to the biodegradation of xenobiotics, and can be used in the treatment of wastewater generated during the production or use of anionic and nonionic surfactants (ASAS and nonionic surfactants).

The aim of the invention is to obtain a new strain with a high destructive activity against toxic xenobiotics, sulfonol (anionic surfactants), sintamide and syntanol (nonionic surfactants).

The strain Pseudoraonas mendocina VKPM B-4710 obtained by selection from the complex of microorganisms of activated sludge. Selection is carried out according to the ability to use surfactants sulphonol, syntanol and syntamide in increasing concentrations. To do this, 50 ml of activated sludge are placed in a 500 ml Erlenmeyer flask. 200 ml of the medium of the following composition, g / dm3, are introduced into the flask containing active sludge: KANROPHO, 7; Mwzof 0, OT, KCl 0,2; M4NO, 0.3; sulfonol 0,2, sintamid. O, 2; Sintanol 0,2. Cultivate pa

rocking chair at 150 rpm and 30 ° C. As the surfactant is destroyed, the concentration of substances is increased by the pouring method; sulfonol - up to 500 mg / dm3, sintanol and syntamide - up to 1000 mg / dm3. The surfactant concentration in the culture medium (the medium is determined by the reaction with methylene blue, the nonionic surfactants by the calorimetric method with phosphomolybdenum acid.

After establishing a constant rate of destruction of the surfactant at an initial concentration in the medium of 500 mg / dm3 of sulphonol and 1000 mg / dm3 of synanol and syntamide, the selection of microorganisms is stopped. 1 ml of the bacterial suspension is taken from the cola, diluted 10,100,1000 and 10,000 times in triplicate on agar medium of the following composition, g / dm3: KjjHPOfl. 0 „7; MgS04 0.2; KS1 0.2, 0.2; 0.3 sulfanol and synthamide 0.3 each, distilled water up to 1 dm3, pH 7.0-7.2. Cups with inoculated microorganisms are cultivated at 28 ° C.

Grown individual colonies of microorganisms are subcultured six times in a dense environment. Biomass of pure cultures is obtained and the ability of isolated bacteria to destroy sulfonol, syntamide and syntanol is studied with growth in a liquid medium of a reduced composition.

As a result of this selection, a strain of Pseudomonas mendocina 2S was isolated, using sulfonol, syntanol, and syntamide as the sole source of carbon and energy.

The destructive activity of the strain Pseudomonas mendocina 2S is studied on a synthetic medium of the following composition, g / dm3: KЈHP04. 0.7; KS1 0.2; N114mi 3 sulfonol 0.1-0.5 syntanol and syntamide 0.6-1.0 at a 1: 1 ratio, distilled water up to 1 dm3, pH 7.0-7.2. Periodic cultivation was carried out under aerobic conditions on a rocking chair at 150 rpm at 30 ° C and the initial concentration of bacterial cells in a medium of 10 7 in 1 ml.

The strain Pseudomonas mendocina 2S deposited in the Central Museum of industrial microorganisms under the number VKPM B-4710, Ytamm has morpholo

go cultural and physiological and biochemical properties.

Morphological and cultural signs. | Colonies of MIIA 3-5 mm in diameter, yellow-orange, round, with equal edges, sometimes split into sector-shaped colonies, a shiny, uniform, oily consistency. The pigment does not diffuse into the medium. Cells are single, rod-shaped, motile. The culture is gram negative. Aerobe. The optimum temperature for growing is 30 ° C, it grows at 41 ° C. On the BCH forms a small ring, weak turbidity of the medium. On arai mineralized medium with sulfonol and nonionic surfactants forms small white-gray colonies (1-2 mm).

Physiological and biochemical properties. It is oxidase-positive, catalazo-positive restores nitrates, does not dilute gelatin, stirs milk. Ureazospozitelna. Forms acid on arabinose, fructose, maltose, sucrose, xylose. Does not grow on galactose, glucose, lactose, ramnose, mannose, inositol, mannitol, sorbitol, dulcite, raffinose. With growth on MPA, it releases hydrogen sulfide and does not release indole. Does not grow in the presence of 5-7% NaCl. Uses pyruvate sodium, sodium lactate. Possesses nitrate reductase.

Example 1. Periodic cultivation of Pseudomonas mendocina 2S is carried out on a model solution of the following composition, g / dm3: KgHPQ j. 0, 7; N% N03 0.3, MgSO,}. 0.01; KC1 0.2 / distilled water up to 1 dm3, pH 7.0. Sulfonol and nonionic surfactants, sintamide, syntanol) are added in concentrations of 100, 200, 300, 400 and 500 mg / dm3 and 600-1000 mg / dm3, respectively. Incubated at 30 ° C on a rocking chair at 150 revolutions per minute

Example 2. Continuous cultivation in laboratory conditions of Pseudomonas mendocina 2S is carried out on model wastewater containing 100-500 mg / dm3 of sulfonic acid and from 600-1000 mg / dm3 of nonionic surfactants (syntanol, syntamide). The required mineral substances are added to the model water, g / dm3: 0.7; 0.3. The process is carried out in a bioreactor under flow culture conditions.

5. 16

To populate the installation bioreactor

the culture is a destructor, the latter is grown on an agarized mineral medium containing up to 300 mg / dm3 of sulfonic acid and 600 mg / dm3 of nonionic surfactants as the sole carbon and energy source, and then the biomass obtained is immobilized on a carrier in the installation. The plant is supplied with a model stock of the following composition, g / dm3: 0.7; NH4N03-0.3; sulfonol 0.5; Nonionic surfactant 0.6-1.0. After 48 hours, the performance of the laboratory wastewater treatment plant is stabilized and the purified water contains sulphonol at a concentration of 4-15 mg / dm3 and nonionic surfactants 5-20 mg / dm3, which does not exceed the maximum allowable concentrations of these compounds for discharge to municipal wastewater treatment plants.

The data of microbiological destruction of sulfonol, syntamide and syntanol with the bacterial strain P, mendocina 2S is given in Table 1.

0

one

55 "

Table 2 presents the data on the purification of real wastewater by the Pseudomonas tnendocina 2S culture under flow conditions.

The use of the proposed strain makes it possible to purify wastewater from anionic surfactants and HI1AB on local structures without first diluting the effluent, and a high degree (96.7%) of purification is achieved with an uplift of up to 500 mg / dm3 and a nonionic surfactant to 1000 mg / dm3. In addition, the Pseudomonas mendocina 2S culture simultaneously purifies wastewater from both sulphonol and sintanol and syntamide.

five

Claims (1)

Invention Formula Bacterial strain Pseudomonas mendocina VKPM B-47JO, used to purify wastewater from sulfonol, sintamide and synthanol.