SU1463761A1 - Method of microbiological treatment of industrial effluents from ampholite surfactants - Google Patents

Abstract

The invention relates to the field of biotechnology, in particular to microbiological treatment of wastewater, and can be used to clean industrial effluent from ampholytic surfactants formed during the production of liquid detergents. The aim of the invention is to improve the quality of wastewater treatment by increasing the amount of ammunition removed. The use of the strain Pseudonronas putida VKPM B-3959, which is capable of completely decomposing amidobetaine, cyclimide and alkylamino-propionate under aerobic conditions in a neutral environment under flow conditions, is the use of the strain. 3 tab. g SL

Description

one

The invention relates to biotechnology, in particular to the microbiological purification of wastewater, and can be used to purify industrial effluents from amphophyte / surface-active substances (AAMA) formed during the production of liquid wet agents (WMS).

The aim of the invention is to expand the technological capabilities of the method.

The method is as follows.

The biomass of the Pseudo strain is grown by putida TO (VKPM B-3959) under aerobic conditions on a dense synthetic medium-trace composition, / g / l: l, l, 0j MgCl 0.1; KCl 0.5, alkylamino-propionate

0.2; cyclimide 0.2; aminobetaine 0,2; alkyl benzene sulfonate 0.1, agar-agar 20.0; water up to 1 l, pH 7.4. Biomass immobilized on a ceramic loading of a birreactor. A drainage flow is carried out in the agrobatic conditions.

Selection strain-destructor.

For the implementation of the proposed method, the selection is carried out selectively by a highly active culture - the destructor Ampav. To do this, the nutrient medium of the following composition, g / l, is passed through the soil, taken on the territory of the plant producing GMR and enriched with AAMA,: 1.0; KCl 0.5; MgCl, 0.1; alkylaminobispro pionag (AABP), in a specific CAA

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31463761

OLG.LT fragile (d 2 mm) convex kolkosti Concentration of AABP is maintained by small “.a,

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H increases from 100 to 300 mg / l, as far as its full utilization is reached, and the outgoing liquid. In this way, an accumulation culture is obtained, which is then immobilized on the loading of the bio-) under flow conditions, gradually increasing the concentration of AABP from 200 to 500 mg / liter of feed, fluid and speed; dilution of the medium, selection of an active strain of bacteria using ABP. as a single source of carbon and energy, Isolation of a pure AABP destructor culture is carried out by sowing the contents of the bioreactor on the mineral medium of the following composition, g / l; l, 0j KC1 0,5j,; AABP 0.4, agar-agar 20.0, distilled water up to 1 L-, pH 7.0. When seeding the contents of the bioreactor, three cultures of microorganisms that can grow on this medium are isolated. These cultures are defined as Pseudoraonas putida TOj Pseudoraonas sp.l, Citro-Ibacter freindii., The destructive activity of the isolated pure cultures of prog I is believed to be in a flow culture. The destructor is considered the microorganism that most actively destroys AABP, as well as cyclide and amidobetaine and n, more quickly reproduce the process of purification from this compound in the words of flow culture.

The ability of microorganisms to destroy amphaletine surfactants under flow culture conditions is shown in; Table 1 ..

  From tab. it follows that the presumptive strain destroys AAMS significantly, but is more active than the rest of the culture.

Memory nJ jntidirvri -

The scientific research institutes are round in shape, the edge is even, the surface is shiny, smooth (S-shape), colonies are transparent with a bluish tint, the color of the surface of the colony and the other side are the same, the zapah is light, the structure of hyaline does not form extracellular pigments. The optimal growth temperature is 30 ° С, it grows at 4 ° С and does not grow when the optimum pH value is neutral. The culture is sensitive to tetracycline, ampicillin, 15 polymyxin B, kanamycin, resistant to chlorafenicol, osmosis and halone-tolerant. On the BCH, there is a parietal ring, a precipitate, the color of the medium does not change; no pigment is released on Wednesday. Prototroph

Samples for oxide azu, catalase and arginine dehydrolase are positive; starch is small and gelatin is not hydrolyzed. With growth on MPA, indole and hydrogen sulfide do not appear.

Relation to carbon sources. Do not use lactose, maltose, sucrose, rhamnose, raf (inozu, inositol, sorbitol, mannitol, salicin, dulcit. 30 Utilizes glucose, arabinose, xylose, fructose, galactose, glycerin, denitrification is absent, it contains yellow-green on the medium with protein.

pigment.

Uses mineral forms that does not need methionine, cysteine, biotin. Plasmid degradation of rDH4 was isolated from strain cells.

Strict aerobic, optimal cultivation temperature - 30 C,

Example 1, Wastewater treatment with Pseudonronas putida is implemented in a bioreactor under flow culture conditions.

but more actively than the rest of the culture populated by the bioreactor culture

in the resulting association. In addition, 45 P, Putida TO cultivates this culture, it produces a purification process 2 times faster than the others, and therefore it was chosen as the ASTA destructor strain.

Morpho-cultural and physiological-biochemical characteristics of the strain-destructor. Movable sticks, with polar-arranged jes-; As long as the cells are located singly or in short chains, gram-negative, do not form spores and capsules, the cell wall is not acid resistant. The cells multiply by simple division. On MPA obradestruktor R, putida TO grown on a dense synthetic medium of the following composition, g / l: 1.0; Sh.Sh), 1.0; MgCl 0.1; KC1 0, rn MPB 0.4, agar-agar 20.0, distilled water up to 1 l. The resulting biomass is immobilized on a ceramic loading unit. The particle size of the ceramic load is about 3-55 m. A model flow of the following composition is supplied to the load: g / l: 0.2; , 0,2; KS1 0.1, APSA 0.5 - After two weeks, the performance of the bioreactor is stabilized and the hIOLG. LTL solid (d 2 mm) convex or small ".a,

0

Memory nJ jntidirvri -

The scientific research institutes are round in shape, the edge is even, the surface is shiny, smooth (S-shape), colonies are transparent with a bluish tint, the color of the surface of the colony and the other side are the same, the zapah is light, the structure of hyaline does not form extracellular pigments. The optimal growth temperature is 30 ° С, it grows at 4 ° С and does not grow when the optimum pH value is neutral. The culture is sensitive to tetracycline, ampicillin, 5 polymyxin B, kanamycin, resistant to chlorafenicol, osmosis and halone-tolerant. On the BCH, there is a parietal ring, a precipitate, the color of the medium does not change. The pigment on the medium is not 0. Prototroph

Samples for oxide azu, catalase and arginine dehydrolase are positive; starch is small and gelatin is not hydrolyzed. With growth on MPA, indole and hydrogen sulfide do not appear.

Relation to carbon sources. Do not use lactose, maltose, sucrose, rhamnose, raf (inozu, inositol, sorbitol, mannitol, salicin, dulcit. 30 Utilizes glucose, arabinose, xylose, fructose, galactose, glycerin, denitrification is absent, it contains yellow-green on the medium with protein.

pigment.

Uses mineral forms that does not need methionine, cysteine, biotin. Plasmid degradation of rDH4 was isolated from strain cells.

Strict aerobic, optimal cultivation temperature - 30 C,

Example 1 A sewage treatment method using Pseudonronas putida is implemented in a bioreactor under flow conditions.

 colonization of the bioreactor

 colonization of the bioreactor

5 P, Putida TO grown by the destructor P, putida TO grown on a dense synthetic medium of the following composition, g / l: 1.0; Sh.Sh), 1.0; MgCl 0.1; KS1 0.5; n MPB 0.4, agar-agar 20.0, distilled water to 1 l. The resulting biomass is immobilized on a ceramic loading unit. The particle size of the ceramic load of about 3-55 mm, with At the load serves model drain of the following composition: g / l: 0.2; , 0,2; KS1 0.1, APSA 0.5, After two weeks, the performance of the bioreactor stabilizes and clears.

Pupped water contains APS of 5–20 mg / l of concentration, which does not exceed the maximum allowable concentration of these compounds for discharge to municipal wastewater treatment plants.

. Table 2 presents the data on the microbiological destruction of AAMB by the strain Pseudomonas putida IBKIM B-3959.

PRI me R 2, the Treatment of real wastewater.

The method of wastewater treatment using Pseudomonas putida TO is implemented in the bioreactor under conditions of flow culture. To populate the bioreactor with the destructor culture of P. putida, TO is grown on synthetic medium of the following composition, g / l: Na2.HP04 1.0; , 1.0 KCl 0.5; MgCl 0.1; alkylaminobisopionate 0.2; cyclimide 0.2; amidobetain 0.2; alkyl benzene sulfonate 0.1; agar-agar 20.0; water up to 1 l; pH 7 | 4. The resulting biomass is immobilized on the bioreactor loading. A real wastewater is supplied to the load, containing: AmAW 200 mg / l; APAV 500 mg / l; suspended solids 40–200 dry residue 70–300 mg / m; sulfates 50-70 mg / m; Fats - up to 0.5 mg / m; pH 6, 5; COD 1300.

Data from the treatment of real wastewater with Pseudomonas putida VKPM B-3959 under flow conditions

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cultivation (dilution rate 0.062) is given in Table 3.

As follows from the table. 1-3 data, the proposed method of microbiological purification of the poet will purify highly concentrated wastewater containing various AAMAS, before; concentrations that allow the discharge of purified water to biological treatment plants.

The use of this strain makes it possible to purify wastewater from APS in local sewage treatment plants 15 without first diluting it and, thus, to prevent AH to enter water bodies. This method allows a high degree of (100%) purification of wastewater from AANP, amidobetaine and cyclimide.

Claims (1)

Invention Formula 25 Method of microbiological treatment of npoMbiFuieHHbix sewage from ampholytic surfactants, which provides for cultivation: in aerobic flow conditions on a biofilter with loading 3Q at pH 6.5-8.5 bacteria strain from the genus PseudoIñnas, characterized in that, in order to expand the technological capabilities of the wastewater treatment method, bacteria are used as bacteria strain 20 35 Pseudomonas putida VKPM B-3959. Table 1 14637618 Continuation of table 1