SU651656A3 - Способ совместного получени инсулина и панкреатина - Google Patents
Способ совместного получени инсулина и панкреатинаInfo
- Publication number
- SU651656A3 SU651656A3 SU752301056A SU2301056A SU651656A3 SU 651656 A3 SU651656 A3 SU 651656A3 SU 752301056 A SU752301056 A SU 752301056A SU 2301056 A SU2301056 A SU 2301056A SU 651656 A3 SU651656 A3 SU 651656A3
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- insulin
- pancreatin
- acetone
- extraction
- joint obtaining
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/94—Pancreatin
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/62—Insulins
- C07K14/625—Extraction from natural sources
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S530/00—Chemistry: natural resins or derivatives; peptides or proteins; lignins or reaction products thereof
- Y10S530/827—Proteins from mammals or birds
- Y10S530/843—Digestive system
- Y10S530/845—Pancreas
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Wood Science & Technology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- Biomedical Technology (AREA)
- Diabetes (AREA)
- Endocrinology (AREA)
- Toxicology (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
Description
Изобретение относитс к фармацевтической промышленности и касаетс получени биологически активных веществ .
Известен способ совместного получени {1нсулина и панкреатина из поджелудочной железы свиньи путем экстракции органическим растворителем с последующим центрифугированием и выделением из экстракта инсулина и из осадка-панкреатина 1.
Однако известный способ не обеспечивает высокого выхода и высокой активности препаратов.
Целью изобретени вл етс повышение выхода и активности препаратов ,
Эта цель достигаетс тем, что экстракцию исходного сырь осзтдествл ют при рН 5-7 40-80%-ным ацетоном в присутствии нейтральных солей, в полученном после центрифугировани осадке устанавливают рН более 5,6 и отдел ют примеси экстракцией 60-70%ным ацетоном, кроме того, экстракцию инсулина из исходного сырь ocyi4ecTвл ют ацетоном при его содержании 5060% ,
Способ осуществл ют следующим образом .
Свежую или консервированную путем быстрого замораживани поджелудочную железу свиньи размельчают и суспендируют в смеси, например., из 24 об.% воды и 76 об.%: ацетона, к которой в случае необходимости добавл ют нейтральные соли, а также соли многоосновных кислот. Дл экстракции и последующего отделени нерастворимых составных частей желез поддерживают концентрацию ацетона в жидкой фазе между 40 и 80 об,%, чтобы было возможным растворение инсулина из ткани желез и дл предотвращени активации проферментов благодар автоматическим процессам в исходной смеси.
Содержание твердого вещества в суспензии составл ет 10-20%, К этой смеси добавл ют соль кальци , лучше всего в форме, водного раствора. Суспензию довод т до.значени -рН 5,7, предпочтительно 5,4-5,6, перемешивают. Врем экстракции составл ет 0,5-1,5 часа. Затем твердые вещества отдел ют путем центрифугировани .
Твердое вещеатво можно экстрагировать многократно, однако дл хорошего
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE2460334A DE2460334C3 (de) | 1974-12-20 | 1974-12-20 | Verfahren zur Gewinnung von Insulin und Pankreatin aus Schweinepankreas |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU651656A3 true SU651656A3 (ru) | 1979-03-05 |
Family
ID=5933942
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU752301056A SU651656A3 (ru) | 1974-12-20 | 1975-12-17 | Способ совместного получени инсулина и панкреатина |
Country Status (30)
| Country | Link |
|---|---|
| US (1) | US4029641A (ru) |
| JP (1) | JPS5186113A (ru) |
| AR (1) | AR208214A1 (ru) |
| AT (1) | AT341078B (ru) |
| BE (1) | BE836952A (ru) |
| BG (1) | BG39801A3 (ru) |
| BR (1) | BR7508415A (ru) |
| CA (1) | CA1057683A (ru) |
| CH (1) | CH628322A5 (ru) |
| CS (1) | CS214870B2 (ru) |
| DD (1) | DD123455A5 (ru) |
| DE (1) | DE2460334C3 (ru) |
| DK (1) | DK141666C (ru) |
| ES (1) | ES443499A1 (ru) |
| FR (1) | FR2295016A1 (ru) |
| GB (1) | GB1536700A (ru) |
| HK (1) | HK39880A (ru) |
| HU (1) | HU176799B (ru) |
| IE (1) | IE42224B1 (ru) |
| IT (1) | IT1051388B (ru) |
| LU (1) | LU74045A1 (ru) |
| NL (1) | NL170804C (ru) |
| NO (1) | NO144040C (ru) |
| NZ (1) | NZ179582A (ru) |
| PL (1) | PL98610B1 (ru) |
| RO (1) | RO66021A (ru) |
| SE (1) | SE436541B (ru) |
| SU (1) | SU651656A3 (ru) |
| YU (1) | YU40130B (ru) |
| ZA (1) | ZA757871B (ru) |
Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2119248A (en) * | 1982-04-28 | 1983-11-16 | John Kenneth Mcmullen | Insulin formulations and method of producing them |
| JPS58175322U (ja) * | 1982-05-12 | 1983-11-24 | 株式会社トヨトミ | 石油ガス化バ−ナ |
| CA2198317C (en) * | 1997-02-24 | 2003-01-07 | Mouhsine El Abboudi | Method for preparing pancreatin which contains low amounts of residual organic solvent and product thereof |
| US7153504B2 (en) * | 2004-07-30 | 2006-12-26 | Can Technologies, Inc. | Stabilized pancreas product |
| CA2812862C (en) | 2010-10-01 | 2018-11-20 | Aptalis Pharma Limited | Enteric coated, low-strength pancrelipase formulations |
| AU2014306222A1 (en) | 2013-08-09 | 2016-02-18 | Aptalis Pharma Ltd. | Digestive enzyme composition suitable for enteral administration |
| KR20170052520A (ko) * | 2013-11-05 | 2017-05-12 | 앨러간 파마슈티컬스 인터내셔널 리미티드 | 고효능 판크레아틴 약학적 조성물 |
Family Cites Families (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US2466487A (en) * | 1946-07-24 | 1949-04-05 | Univ Alberta | Process of obtaining insulin from pancreas glands |
| US2573099A (en) * | 1946-10-23 | 1951-10-30 | Novo Terapeutisk Labor As | Method of recovering proteolytic enzymes from mammalian pancreas glands with urea |
| US2779706A (en) * | 1950-08-12 | 1957-01-29 | Organon | Insulin extraction |
| US2752286A (en) * | 1952-09-11 | 1956-06-26 | Armour & Co | Extraction of insulin from pancreas tissue |
| US2897117A (en) * | 1955-02-10 | 1959-07-28 | Univ Alberta | Insulin process |
| NL6816599A (ru) * | 1968-11-20 | 1970-05-22 |
-
1974
- 1974-12-20 DE DE2460334A patent/DE2460334C3/de not_active Expired
-
1975
- 1975-01-01 AR AR261680A patent/AR208214A1/es active
- 1975-12-15 ES ES443499A patent/ES443499A1/es not_active Expired
- 1975-12-15 NL NLAANVRAGE7514576,A patent/NL170804C/xx not_active IP Right Cessation
- 1975-12-16 RO RO7584224A patent/RO66021A/ro unknown
- 1975-12-16 BG BG7531806A patent/BG39801A3/xx unknown
- 1975-12-17 HU HU75HO1863A patent/HU176799B/hu unknown
- 1975-12-17 CH CH1637475A patent/CH628322A5/de not_active IP Right Cessation
- 1975-12-17 CS CS758639A patent/CS214870B2/cs unknown
- 1975-12-17 SU SU752301056A patent/SU651656A3/ru active
- 1975-12-18 YU YU3229/75A patent/YU40130B/xx unknown
- 1975-12-18 DD DD190297A patent/DD123455A5/xx unknown
- 1975-12-18 US US05/641,927 patent/US4029641A/en not_active Expired - Lifetime
- 1975-12-18 LU LU74045A patent/LU74045A1/xx unknown
- 1975-12-18 NZ NZ179582A patent/NZ179582A/xx unknown
- 1975-12-18 IT IT30480/75A patent/IT1051388B/it active
- 1975-12-18 BR BR7508415*A patent/BR7508415A/pt unknown
- 1975-12-19 JP JP50150667A patent/JPS5186113A/ja active Granted
- 1975-12-19 IE IE2770/75A patent/IE42224B1/en unknown
- 1975-12-19 FR FR7539201A patent/FR2295016A1/fr active Granted
- 1975-12-19 DK DK581375A patent/DK141666C/da not_active IP Right Cessation
- 1975-12-19 PL PL1975185730A patent/PL98610B1/pl unknown
- 1975-12-19 CA CA242,359A patent/CA1057683A/en not_active Expired
- 1975-12-19 ZA ZA757871A patent/ZA757871B/xx unknown
- 1975-12-19 SE SE7514448A patent/SE436541B/xx not_active IP Right Cessation
- 1975-12-19 AT AT968675A patent/AT341078B/de not_active IP Right Cessation
- 1975-12-19 NO NO754325A patent/NO144040C/no unknown
- 1975-12-22 GB GB52458/75A patent/GB1536700A/en not_active Expired
- 1975-12-22 BE BE163015A patent/BE836952A/xx not_active IP Right Cessation
-
1980
- 1980-07-31 HK HK398/80A patent/HK39880A/xx unknown
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