SU515471A3 - Способ количественного определени энзимов гидролитического действи - Google Patents
Способ количественного определени энзимов гидролитического действиInfo
- Publication number
- SU515471A3 SU515471A3 SU1819712A SU1819712A SU515471A3 SU 515471 A3 SU515471 A3 SU 515471A3 SU 1819712 A SU1819712 A SU 1819712A SU 1819712 A SU1819712 A SU 1819712A SU 515471 A3 SU515471 A3 SU 515471A3
- Authority
- SU
- USSR - Soviet Union
- Prior art keywords
- solution
- buffer
- trypsin
- substrate
- enzymes
- Prior art date
Links
- 102000004190 Enzymes Human genes 0.000 title claims description 15
- 108090000790 Enzymes Proteins 0.000 title claims description 15
- 230000003301 hydrolyzing effect Effects 0.000 title claims description 4
- 238000000034 method Methods 0.000 title claims description 4
- 239000000758 substrate Substances 0.000 claims description 12
- 239000000243 solution Substances 0.000 claims description 9
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 9
- 239000007853 buffer solution Substances 0.000 claims description 8
- 239000000872 buffer Substances 0.000 claims description 7
- DNIAPMSPPWPWGF-UHFFFAOYSA-N Propylene glycol Chemical compound CC(O)CO DNIAPMSPPWPWGF-UHFFFAOYSA-N 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 6
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 claims description 2
- 239000007983 Tris buffer Substances 0.000 claims description 2
- 239000003795 chemical substances by application Substances 0.000 claims description 2
- 229910052708 sodium Inorganic materials 0.000 claims description 2
- 239000011734 sodium Substances 0.000 claims description 2
- 239000000126 substance Substances 0.000 claims description 2
- LENZDBCJOHFCAS-UHFFFAOYSA-N tris Chemical compound OCC(N)(CO)CO LENZDBCJOHFCAS-UHFFFAOYSA-N 0.000 claims description 2
- 102000004142 Trypsin Human genes 0.000 claims 3
- 108090000631 Trypsin Proteins 0.000 claims 3
- 150000001408 amides Chemical class 0.000 claims 3
- 239000012588 trypsin Substances 0.000 claims 3
- 102000004225 Cathepsin B Human genes 0.000 claims 2
- 108090000712 Cathepsin B Proteins 0.000 claims 2
- 230000001810 trypsinlike Effects 0.000 claims 2
- 125000000539 amino acid group Chemical group 0.000 claims 1
- 125000000637 arginyl group Chemical group N[C@@H](CCCNC(N)=N)C(=O)* 0.000 claims 1
- 229920001577 copolymer Polymers 0.000 claims 1
- 238000004090 dissolution Methods 0.000 claims 1
- FBYVDJCBBGMJHI-UHFFFAOYSA-N n-methoxynaphthalen-2-amine Chemical compound C1=CC=CC2=CC(NOC)=CC=C21 FBYVDJCBBGMJHI-UHFFFAOYSA-N 0.000 claims 1
- 230000028327 secretion Effects 0.000 claims 1
- 150000001875 compounds Chemical class 0.000 description 8
- 230000000694 effects Effects 0.000 description 8
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 description 2
- OGMADIBCHLQMIP-UHFFFAOYSA-N 2-aminoethanethiol;hydron;chloride Chemical compound Cl.NCCS OGMADIBCHLQMIP-UHFFFAOYSA-N 0.000 description 2
- JBIJLHTVPXGSAM-UHFFFAOYSA-N 2-naphthylamine Chemical compound C1=CC=CC2=CC(N)=CC=C21 JBIJLHTVPXGSAM-UHFFFAOYSA-N 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 230000007062 hydrolysis Effects 0.000 description 2
- 238000006460 hydrolysis reaction Methods 0.000 description 2
- 239000002244 precipitate Substances 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- YNJBWRMUSHSURL-UHFFFAOYSA-N trichloroacetic acid Chemical compound OC(=O)C(Cl)(Cl)Cl YNJBWRMUSHSURL-UHFFFAOYSA-N 0.000 description 2
- 102000003902 Cathepsin C Human genes 0.000 description 1
- 108090000267 Cathepsin C Proteins 0.000 description 1
- 101000930822 Giardia intestinalis Dipeptidyl-peptidase 4 Proteins 0.000 description 1
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 239000007864 aqueous solution Substances 0.000 description 1
- 239000007978 cacodylate buffer Substances 0.000 description 1
- 238000005119 centrifugation Methods 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- 230000007071 enzymatic hydrolysis Effects 0.000 description 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000002223 garnet Substances 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 238000011534 incubation Methods 0.000 description 1
- 125000001288 lysyl group Chemical group 0.000 description 1
- JVXXKQIRGQDWOJ-UHFFFAOYSA-N naphthalene-2-carboxamide Chemical compound C1=CC=CC2=CC(C(=O)N)=CC=C21 JVXXKQIRGQDWOJ-UHFFFAOYSA-N 0.000 description 1
- BHAAPTBBJKJZER-UHFFFAOYSA-N p-anisidine Chemical compound COC1=CC=C(N)C=C1 BHAAPTBBJKJZER-UHFFFAOYSA-N 0.000 description 1
- AFAIELJLZYUNPW-UHFFFAOYSA-N pararosaniline free base Chemical compound C1=CC(N)=CC=C1C(C=1C=CC(N)=CC=1)=C1C=CC(=N)C=C1 AFAIELJLZYUNPW-UHFFFAOYSA-N 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- IHQKEDIOMGYHEB-UHFFFAOYSA-M sodium dimethylarsinate Chemical compound [Na+].C[As](C)([O-])=O IHQKEDIOMGYHEB-UHFFFAOYSA-M 0.000 description 1
- 238000002798 spectrophotometry method Methods 0.000 description 1
- 150000004992 toluidines Chemical class 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/08—Tripeptides
- C07K5/0802—Tripeptides with the first amino acid being neutral
- C07K5/0804—Tripeptides with the first amino acid being neutral and aliphatic
- C07K5/0806—Tripeptides with the first amino acid being neutral and aliphatic the side chain containing 0 or 1 carbon atoms, i.e. Gly, Ala
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Health & Medical Sciences (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Life Sciences & Earth Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Peptides Or Proteins (AREA)
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US17228071A | 1971-08-16 | 1971-08-16 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SU515471A3 true SU515471A3 (ru) | 1976-05-25 |
Family
ID=22627041
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SU1819712A SU515471A3 (ru) | 1971-08-16 | 1972-08-15 | Способ количественного определени энзимов гидролитического действи |
Country Status (8)
| Country | Link |
|---|---|
| JP (2) | JPS5621400B2 (enrdf_load_stackoverflow) |
| CA (1) | CA1007555A (enrdf_load_stackoverflow) |
| CH (1) | CH569281A5 (enrdf_load_stackoverflow) |
| DE (1) | DE2239936C2 (enrdf_load_stackoverflow) |
| FR (1) | FR2150146A5 (enrdf_load_stackoverflow) |
| GB (1) | GB1404195A (enrdf_load_stackoverflow) |
| SE (1) | SE401738B (enrdf_load_stackoverflow) |
| SU (1) | SU515471A3 (enrdf_load_stackoverflow) |
Families Citing this family (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4215047A (en) * | 1977-06-06 | 1980-07-29 | Ajinomoto Company Incorporated | 7-(Arginylamino)-4-methylcoumarins |
| CA1087075A (en) * | 1977-08-05 | 1980-10-07 | Robert J. Gargiulo | Composition and method for determining transferase and protease activity |
| JPS54103078A (en) * | 1978-01-31 | 1979-08-14 | Eon Denki Keiki Kk | Movable coil type indication electric instrument |
| DE2905531A1 (de) * | 1979-02-14 | 1981-01-08 | Boehringer Mannheim Gmbh | Diagnostisches mittel zum nachweis von leukozyten in koerperfluessigkeiten |
| JPS5925699A (ja) * | 1982-08-03 | 1984-02-09 | Torii Yakuhin Kk | 酵素活性測定方法 |
| JPS59192724A (ja) * | 1983-04-16 | 1984-11-01 | Toyobo Co Ltd | 耐光性合成繊維の製造方法 |
| JPS6088130A (ja) * | 1983-10-14 | 1985-05-17 | Toyobo Co Ltd | 着色繊維の製造法 |
| JPS60158598A (ja) * | 1984-01-30 | 1985-08-19 | Hotsukusu Denshi Kogyo Kk | 遠隔制御・監視装置 |
| JPS647800U (enrdf_load_stackoverflow) * | 1987-07-01 | 1989-01-17 | ||
| JP2000086305A (ja) * | 1998-09-17 | 2000-03-28 | Nippon Sheet Glass Co Ltd | ガラスパネル |
| US6833186B2 (en) | 2002-04-10 | 2004-12-21 | Ppg Industries Ohio, Inc. | Mineral-filled coatings having enhanced abrasion resistance and wear clarity and methods for using the same |
-
1972
- 1972-08-01 CA CA148,465A patent/CA1007555A/en not_active Expired
- 1972-08-10 GB GB3735972A patent/GB1404195A/en not_active Expired
- 1972-08-14 DE DE19722239936 patent/DE2239936C2/de not_active Expired
- 1972-08-15 CH CH1207172A patent/CH569281A5/xx not_active IP Right Cessation
- 1972-08-15 SU SU1819712A patent/SU515471A3/ru active
- 1972-08-16 JP JP8149072A patent/JPS5621400B2/ja not_active Expired
- 1972-08-16 FR FR7229379A patent/FR2150146A5/fr not_active Expired
- 1972-08-16 SE SE1064772A patent/SE401738B/xx unknown
-
1979
- 1979-08-16 JP JP10451379A patent/JPS5533475A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JPS4829497A (enrdf_load_stackoverflow) | 1973-04-19 |
| CH569281A5 (enrdf_load_stackoverflow) | 1975-11-14 |
| DE2239936C2 (de) | 1983-02-10 |
| DE2239936A1 (de) | 1973-03-01 |
| JPS5621400B2 (enrdf_load_stackoverflow) | 1981-05-19 |
| CA1007555A (en) | 1977-03-29 |
| SE401738B (sv) | 1978-05-22 |
| JPS5533475A (en) | 1980-03-08 |
| FR2150146A5 (enrdf_load_stackoverflow) | 1973-03-30 |
| GB1404195A (en) | 1975-08-28 |
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