SU1681807A1 - Method for increasing resistance of corn to cold - Google Patents

Abstract

This invention relates to agriculture. The purpose of the invention is to increase the efficiency of protecting plants from cold. 00- and Qg-ubiquinones at a concentration of 0.0001-0.05% were used as a cryoprotectant. The effectiveness of ubiquinones has been established by treating the seeds and vegetative corn plants in the phase of three leaves. The use of ubiquinones makes it possible to increase the resistance of plants to cold, as evidenced by yield data — the yield increase from the use of ubiquinone is 16–29%. 3 tab.

Description

The invention relates to agriculture and can be used to improve the cold tolerance of plants.

The aim of the invention is to increase the efficiency of protecting plants from cold.

Two types of ubiquinones, ubiquinone Qo and ubiquinone Qg, at concentrations of 0.0001-0.05% were used as a cryol protector. The effectiveness of ubiquinones has been established by treating the seeds and vegetative corn plants in the 3-leaf phase.

As a reference, a known plant growth regulator chloro-chloride (С С С) was used as a standard, which increases the resistance of plants to cold. CCC was used in a concentration of 0.02-0.5%.

Pr and m in p. In the growing season, plants of the non-cold-resistant corn hybrid P3978 were cultivated

Ubiquinone and CAS for the purpose of comparative study of the effect of these drugs on the final productivity of plants that have experienced low-temperature effects in the initial phases of development. Processing was carried out as follows.

Before sowing, the seeds were soaked for 12 hours in a 0.5% solution of CCC, or germinated on rastitel in a thermostat at 27 ° C for 24 hours in a 0.02% solution of CCC. The latter was done in order to harmonize the CAS treatment technology with the applied methods of preparing seeds for sowing and processing with ubiquinone, which is necessary for comparative analysis.

The treatment with ubiquinone was carried out in two ways. In the first case, the seeds were germinated in distilled water (as well as the control), and the treatment was carried out after sowing on the 1-3-leaf phase of the instillation. 00

00

about VI

in the plant socket during the 2 days preceding the decrease in temperature, and on the first day of low-temperature exposure. In the second case, the seeds were sown before sowing for 24 hours at 27 ° С in a solution of ubiquinone.

Low-temperature exposure was carried out by placing plants on the phase of 3 leaves in an artificial climate unit for 3 days at + 4 ° C. Next, the plants were again transferred to the growing area and grown to full maturity.

In each variant, there were 5 replicates (2 plants each). One repetition corresponded to one 40-pound vegetation vessel.

Observation of the development of seedlings showed that ubiquinone, like the CAS, has a retardant effect on corn seeds. The ubiquinone-treated plants were noticeably delayed in their development, and this ignition was all the more significant the higher the solution concentration. Seeds germinated on a solution concentration of 0.05%, practically did not grow.

The dynamics of seed germination of corn treated with CAS and ubiquinone are presented in Table 1.

Five days after the end of the low-temperature exposure, the state of the plants was analyzed (percentage of plants without visible damage to the leaves, leaf area, height of the plants). The results are shown in Table. 2

All used exogenous techniques increased the percentage of plants without damage compared with the control. It can be noted that in processing the seeds of the CAS, both options gave similar results. The instillation of ubiquinone has even more favorably affected the state of the plants (especially Qo). Minimal damage to plants is observed when the seeds are soaked in ubiquinone solutions.

Correct analysis of data on leaf surface and plant height

it is possible only for the variants in which the treatment was carried out by instillation, since when the seeds are locked in solutions of the CAS and ubiquinone solutions, an inhibitory effect is observed and ultimately occurs

the overall result of both low-temperature damage and inhibition. But even in these cases, it can be noted that when the seeds are soaked in ubiquinone solutions in a concentration of 0.001% (variants 10 and

12) plant growth in the recovery period after stress was more intense than in control, which also indicates a lower degree of damage to the treated plants.

The data of the final yield of the experiment are given in table. 3

It can be noted that all the types of exogenous treatment used had a positive effect on the final productivity of plants, and if the treatment of CAS seeds led to an increase in the grain weight only by 5%, then with the instillation of the ubiquinone solution, the gain for Qg and Q0 was 16 and 29%, respectively maximum of

test concentrations (0.0001%). Soaking the seeds for the ubiquinone solution resulted in maximum yield increases: 17% and 20% respectively for Qg and Q0. Analyzing the data, you can

to come to the conclusion that ubiquinone is better than CAS, it protects plants from the damaging effects of low temperature.

Thus, the use of ubiquinones Qo and Qg increases the resistance of maize plants to cold.

Formula of the Invention The use of ubiquinones Q0 and Qg as a means for improving the cold tolerance of corn.

Table

Table

2b

Table 3

Claims (2)

Claim The use of ubiquinones Q _o and Qg as a means to increase the cold hardiness of corn. Table 1 Option Seedlings 05/16/89 05/17/89 05/19/89 113978- control 74 98 100 113978 + CVS (0.2%) 55 96 96 113978 + CVS (0.5%) 12 87 87 113978 + Qs (0.001%) fifty 96 1Q0 113978 + Qg (0.016%) 0 79 100 113978+ Qg (0.05%) 0 4 4 113978+ Qo (0.016%) 41 96 100 113978+ Qo (0.016%) 0 fifty 83 113978+ Qo (0.05%) 0 16 42 Table Option Option Description Processing method Plants without damage,% The area of the leaf surface of 1 plant, cm ³ Plant height, cm 1 113978 - control - 25 27.3 + 1.8 11.7 ± 0.4 2 113978+ CVS (0.2%) Soak 55 30.1 ± 0.9 11.9 ± 0.4 3 113978+ CVS (0.5%) __. fifty 25.0 ± 1.0 10.5 ± 0.3 4 113978 + 09 (0.0001%) Burying fifty 28.4 ± 1.4 11.8 ± 0.3 5 113978 + Qg (0.001%) Also fifty 30, 4 ± 1.1 12.2 ± 0.2 6 113978 + 09 (0.016%) 65 32.8 ± 0.8 13.2 ± 0.2 1 , 113978+ Qo (0.0001%) "1 79 37.5 ± 1.1 13.6 ± 0.3 8 . 113978+ Qo (0.001%) tl___ . 75 32.2 ± 0.6 12.6 ± 0.4 9 113978+ Qo (0.016%) 74 32.7 ± 1.5 12.2 ± 0.3 10 113978 + 09 (0.001%) Soak 83 29.9 ± 2.0 11.9 ± 0.5 eleven 113978 + 09 (0.016%) m 90 26.9 ± 1.5 11.2 ± 0.3 12 113978 + Qo (0.001%) 90 32.1 ± 0.9 12.8 ± 0.4 thirteen 113978+ Qo (0.016%) ___il___ 84 26.6 ± 0.8 10.4 ± 0.3 14 113978+ Qo (0.05%) eleven___ 83 22.2 ± 1.3 10.0 ± 0.5 2b table 3 Option Option Description Processing method Absolutely dry grain weight with a cob g % to control 1 113978 - control - 81.3 ± 5.2 - 2 113978+ CVS (0.02%) Soak 83.5 ± 3.3 103 3 113978+ CVS (0.5%) _n__ 85.0 ± 6.0 105 4 113978 + Qg (0.0001%) Burying 94.0 ± 5.7 116 5 113978 + 09 (0.001%) Also 88.4 ± 2.8 109 6 113978 + 09 (0.016%) - ^and - 91.5 ± 3.4 113 7 113978+ Qo (0.0001%) , and 105.3 ± 6.1 129 8 113978+ Qo (0.001%) n 91.4 ± 5.1 • 112 9 113978+ Qo (0.016%) n 91.4 ± 5.1 112 10 113978 + 09 (0.001%) Soak 88.6 + -5.3 109 eleven 113978 + 09 (0.016%) η 95.1 ± 4.0 117 12 113978+ Qo (0.001%) 97.8 + 8.2 120 thirteen 113978 + Qo (0.016%) 91.3 + 5.9 112 14 113978 + Qo (0.005%) 89.4 ± 4.5 110