SU1066603A1 - Method of preparing oleuropein - Google Patents

Abstract

A method of producing oleuropein by extracting raw olives with lower alcohol, followed by evaporation and processing of the obtained extract with organic solvents and isolating the target product, characterized in that, in order to increase the yield of the target product and simplify the process, extraction is carried out with 90% ethyl alcohol and the resulting extract is treated with acetone, and the selection of the target product is carried out with a mixture of alcohol-chloroform at a ratio of 1: 2-1: 4.

Description

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This invention relates to the recovery of oleuropein from raw olives, which can be used in the pharmaceutical industry.

A known method for the release of the substance "Oleuropein, which gives bitterness to the olives. Oleuropein is extracted from oliv, to separate from tannin extract p iCTHopVi oT in water, add a 5% solution of x, 1 N of Na in NaCl and then with a large excess; gelatin is precipitated onto it. Fi. Ebinapiibo yut and the remaining solids are dissolved: In aceto-water (1: 1). The solution is passed through a column of A1 O to adsorb flavonoids 1.

A method for isolating oleuropein is obtained by extracting defatted pulp of mature olives with methanol, followed by evaporation of methanol and dissolving the residue in water, then the sugar is removed from the aqueous solution by extraction with ethyl acetate, the remaining extract is subjected to separation on a chromatographic column with polyamide, then the glycoside is washed with water and lyophilized. oleuropein 2 is released.

However, the known method does not provide a sufficiently high yield of oleuropein.

The aim of the invention is to increase the target product and simplify the process.

The goal is achieved by the fact that according to the method for producing oleuropein PUT. scrubbing of 1 com; 1 oh mash processing with lower alcohol followed by evaporation: 1 treatment of the extract with organic solvents and extraction of the target product, extraction is carried out with 10% ethyl alcohol and the resulting extract is treated acet), and the selection of the desired product was dissolved with an alcohol-chloroform mixture at -ooTHOoiennn 1: 2-1: 4.

The method is carried out in the following way.

The bone of the raw olives is degreased in the Soxhlet apparatus, grilled; her

extracted 90 °

this solution: 1c), I - extract is concentrated at. using rotary evaporator. Sugar from the concentrated ethanol extract precipitates with acetone, followed by separation of oleuropein from a solution of ethanol-chlorine (roform 1: 2-1: 4.

Example. 3 kg of non-fat dried pulp of untreated olives are extracted several times with a 90 / o-ethanol solution. The combined drain is concentrated at 45 ° C using a rotary evaporator. Acetopic precipitated sugar until complete loss. Oleuropein is extracted from the acetone solution by treatment with a mixture of alcohol-chloroform (1: 3).

The proposed method allows to obtain the yield of the target product 0, by weight. M which is compared with 0.095 / r1-ty. 1 output by the method prototype.

Claims (1)

METHOD FOR PRODUCING OLEUROPEIN by extracting the pulp of untreated olives with lower alcohol, followed by evaporation and processing of the obtained extract with organic solvents and isolating the target product, characterized in that, in order to increase the yield of the target product and simplify the process, the extraction is carried out with a 90% solution of ethyl alcohol and the obtained the extract is treated with acetone, and the selection of the target product is carried out with a mixture of alcohol-chloroform in a ratio of 1: 2-1: 4.