SU1736501A1 - Method of oleuropeine preparation - Google Patents

Abstract

Usage: The invention relates to the chemical and pharmaceutical industry and relates to a method for producing oleurophen from vegetable raw materials. The purpose of the invention: improving the purity of the target product. SUMMARY OF THE INVENTION: The method is carried out by extracting freshly harvested European olives with acetone, evaporation of the extract, sequential treatment with chloroform, ether and ethyl acetate, dehydration of the resulting extraction, re-evaporation, purification on alumina, eluting the target product with ethyl acetate-acetone, treating the eluate with activated coal and evaporation. Positive effect: The product obtained is homogeneous by chromatography and is freed from sterols, triterpene acids and sugars. 1 tab.

Description

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This invention relates to the chemical and pharmaceutical industry and relates to a process for the production of oleuropein from vegetable raw materials.

The aim of the invention is to increase the purity of the target product.

Example 1, 1 kg of freshly harvested olives of European Olea europaea is crushed, about 50.0 g of CaCO3 is added, extracted with acetone in a ratio of 1:10 at room temperature. After 12 h, the acetone extract is decanted. Extraction was carried out three more times under the same conditions with a new portion of acetone. The extracts are combined, evaporated to a water residue at 35 ° C using a rotary evaporator. The aqueous residue is treated successively with chloroform, ether and ethyl acetate. The ethyl acetate extracts are dehydrated, evaporated to 0.5 volume and chromatographed on anhydrous, neutral, without a degree of activity of alumina in a ratio of 1:90. The column is washed with ethyl acetate until the last portion of the eluate is discolored. After that, the column is washed with a mixture of ethyl acetate-acetone in a ratio of 1: 2. The process was monitored by TLC in an ethyl acetate-methanol-water system (100: 16.5: 13.5). The plate is sprayed with a 3% alcoholic solution of ferric chloride. The same eluates are combined, treated with activated carbon, filtered and evaporated on a rotary evaporator at 35 ° C until the odor of the solvent has completely disappeared and dried.

The output of oleuropein by the proposed method of 1.19%.

Example 2. The method is carried out according to Example 1, only purification is carried out at an extract: sorbent ratio of 1:95, and elution with ethyl acetate-acetone is 1: 2.25. The yield of the target product is 1.63%.

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Example 3. The method is carried out as in Example 1, only the purification is carried out at an extract: sorbent ratio of 1: 100, and elution with a mixture of ethyl acetate-acetone is 1: 2.5. The yield of the target product is 1.78%.

The output of oleuropein by the proposed method and the prototype presented in the table.

Compared to the prototype oleuropein, the target product obtained by the proposed method has only one spot on the chromatogram, since it is freed from sterols, triterpenic acids and sugars.

Oleuropein is a chromatographically homogeneous substance, composition С25Нз201з 1.5 NaO. sf - 145 ° (1.0, methanol). In the IR spectrum there are absorption bands at 340 (hydroxyl group), 1720 (ester

group), 1640 and 1535 (aromatic draw).

Claims (1)

Claim method for producing oleuropein by extraction of plant raw material with an organic solvent, evaporation and purification, characterized in that, to increase the purity of the target product, freshly harvested European olive leaves (Olea europaea) are used as raw materials, extraction is carried out four times with acetone, one stripped off residue is treated successively with chloroform, ether, ethyl acetate . m, the ethyl acetate extract is dehydrated, re-evaporated and the purification is carried out on alumina in the ratio of sorbent: extract 1: (90-100), the target product is eluted with an ethyl acetate mixture t-acetone 1: (2.2.5), the eluate is treated with activated carbon and evaporated.