SI3087179T1 - Fuzijski polipeptidi z izboljšanimi lastnostmi, povezani z omega hidroksilazo - Google Patents

Fuzijski polipeptidi z izboljšanimi lastnostmi, povezani z omega hidroksilazo

Info

Publication number
SI3087179T1
SI3087179T1 SI201531270T SI201531270T SI3087179T1 SI 3087179 T1 SI3087179 T1 SI 3087179T1 SI 201531270 T SI201531270 T SI 201531270T SI 201531270 T SI201531270 T SI 201531270T SI 3087179 T1 SI3087179 T1 SI 3087179T1
Authority
SI
Slovenia
Prior art keywords
hydroxylase
omega
improved properties
fusion polypeptides
related fusion
Prior art date
Application number
SI201531270T
Other languages
English (en)
Inventor
Baolong Zhu
Andreas W. Schirmer
Cindy Chang
Original Assignee
Genomatica, Inc.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genomatica, Inc. filed Critical Genomatica, Inc.
Publication of SI3087179T1 publication Critical patent/SI3087179T1/sl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/64—Fats; Fatty oils; Ester-type waxes; Higher fatty acids, i.e. having at least seven carbon atoms in an unbroken chain bound to a carboxyl group; Oxidised oils or fats
    • C12P7/6409—Fatty acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y114/00—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14)
    • C12Y114/15—Oxidoreductases acting on paired donors, with incorporation or reduction of molecular oxygen (1.14) with reduced iron-sulfur protein as one donor, and incorporation of one atom of oxygen (1.14.15)
    • C12Y114/15003—Alkane 1-monooxygenase (1.14.15.3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/01—Carboxylic ester hydrolases (3.1.1)
    • C12Y301/01005—Lysophospholipase (3.1.1.5)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)
    • C12Y301/02—Thioester hydrolases (3.1.2)
    • C12Y301/02014—Oleoyl-[acyl-carrier-protein] hydrolase (3.1.2.14), i.e. ACP-thioesterase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
SI201531270T 2014-06-16 2015-06-16 Fuzijski polipeptidi z izboljšanimi lastnostmi, povezani z omega hidroksilazo SI3087179T1 (sl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201462012970P 2014-06-16 2014-06-16
PCT/US2015/036078 WO2015195697A1 (en) 2014-06-16 2015-06-16 Omega-hydroxylase-related fusion polypeptides with improved properties
EP15732504.4A EP3087179B1 (en) 2014-06-16 2015-06-16 Omega-hydroxylase-related fusion polypeptides with improved properties

Publications (1)

Publication Number Publication Date
SI3087179T1 true SI3087179T1 (sl) 2020-10-30

Family

ID=53491691

Family Applications (1)

Application Number Title Priority Date Filing Date
SI201531270T SI3087179T1 (sl) 2014-06-16 2015-06-16 Fuzijski polipeptidi z izboljšanimi lastnostmi, povezani z omega hidroksilazo

Country Status (14)

Country Link
US (3) US11441130B2 (sl)
EP (2) EP3087179B1 (sl)
JP (3) JP6826435B2 (sl)
KR (2) KR102663049B1 (sl)
CN (2) CN106604990B (sl)
AU (2) AU2015277261B2 (sl)
BR (1) BR112016029235B1 (sl)
CA (1) CA2951925A1 (sl)
ES (1) ES2803560T3 (sl)
MX (1) MX2016016565A (sl)
MY (1) MY198268A (sl)
PL (1) PL3087179T3 (sl)
SI (1) SI3087179T1 (sl)
WO (1) WO2015195697A1 (sl)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
SI2984490T1 (sl) 2013-06-14 2020-10-30 Genomatica, Inc. Metode proizvodnje derivatov omega hidroksilirane maščobne kisline
EP3087179B1 (en) * 2014-06-16 2020-04-08 Genomatica, Inc. Omega-hydroxylase-related fusion polypeptides with improved properties
WO2017101987A1 (en) * 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties
CA3058950A1 (en) 2017-04-03 2018-10-11 Genomatica, Inc. Thioesterase variants having improved activity for the production of medium-chain fatty acid derivatives
WO2020047304A1 (en) 2018-08-31 2020-03-05 Genomatica, Inc. Xylr mutant for improved xylose utilization or improved co-utilization of glucose and xylose

Family Cites Families (58)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1305083C (en) 1987-02-18 1992-07-14 Yoshiharu Kimura Microorganism belonging to genus rhodococcus, and a process for producing alkene derivative and unsaturated fatty acid
US5000000A (en) 1988-08-31 1991-03-19 University Of Florida Ethanol production by Escherichia coli strains co-expressing Zymomonas PDC and ADH genes
US5028539A (en) 1988-08-31 1991-07-02 The University Of Florida Ethanol production using engineered mutant E. coli
US5482846A (en) 1988-08-31 1996-01-09 University Of Florida Ethanol production in Gram-positive microbes
US5424202A (en) 1988-08-31 1995-06-13 The University Of Florida Ethanol production by recombinant hosts
WO1991016427A1 (en) 1990-04-24 1991-10-31 Stratagene Methods for phenotype creation from multiple gene populations
US5602030A (en) 1994-03-28 1997-02-11 University Of Florida Research Foundation Recombinant glucose uptake system
US6428767B1 (en) 1995-05-12 2002-08-06 E. I. Du Pont De Nemours And Company Method for identifying the source of carbon in 1,3-propanediol
US5939250A (en) 1995-12-07 1999-08-17 Diversa Corporation Production of enzymes having desired activities by mutagenesis
US5965408A (en) 1996-07-09 1999-10-12 Diversa Corporation Method of DNA reassembly by interrupting synthesis
US7576227B2 (en) 2002-04-29 2009-08-18 Union Carbide Chemicals & Plastics Technology Corporation Integrate chemical processes for industrial utilization of seed oils
EP2017334A3 (en) 2004-11-10 2009-04-15 KNC Laboratories Co., Ltd Method of isolating P450 Gene
US8110670B2 (en) 2006-05-19 2012-02-07 Ls9, Inc. Enhanced production of fatty acid derivatives
EP2024491B1 (en) 2006-05-19 2014-11-19 LS9, Inc. Production of fatty acids and derivatives thereof
US20100242345A1 (en) 2006-05-19 2010-09-30 LS9, Inc Production of fatty acids & derivatives thereof
DK2121546T3 (en) 2006-10-13 2018-03-12 Elevance Renewable Sciences Process for preparing omega-dicarboxylic acid olefin derivative by metathesis
EP2076484B1 (en) 2006-10-13 2020-01-08 Elevance Renewable Sciences, Inc. Synthesis of terminal alkenes from internal alkenes via olefin metathesis
US8110093B2 (en) 2007-03-14 2012-02-07 Ls9, Inc. Process for producing low molecular weight hydrocarbons from renewable resources
AU2008230735A1 (en) 2007-03-28 2008-10-02 Ls9, Inc. Enhanced production of fatty acid derivatives
US20080293060A1 (en) 2007-04-23 2008-11-27 Ls9, Inc. Methods and Compositions for Identification of Hydrocarbon Response, Transport and Biosynthesis Genes
CN101809146B (zh) 2007-05-22 2013-12-11 Ls9公司 产烃基因及其使用方法
JP5324129B2 (ja) 2007-05-25 2013-10-23 神戸天然物化学株式会社 Ｃｙｐ１５３による芳香族化合物の製造方法
US20100199548A1 (en) 2007-07-06 2010-08-12 Ls9, Inc. Systems and methods for the production of fatty esters
FR2921362B1 (fr) 2007-09-20 2012-09-21 Arkema France Procede de synthese d'acides gras omega-insatures
AU2008304180B2 (en) 2007-09-27 2014-02-27 Genomatica, Inc. Reduction of the toxic effect of impurities from raw materials by extractive fermentation
TWI351779B (en) 2007-12-03 2011-11-01 Advance Smart Ind Ltd Apparatus and method for correcting residual capac
WO2009085278A1 (en) 2007-12-21 2009-07-09 Ls9, Inc. Methods and compositions for producing olefins
CA2722441C (en) 2008-05-16 2021-01-26 Ls9, Inc. Methods and compositions for producing hydrocarbons
US8232924B2 (en) 2008-05-23 2012-07-31 Alliant Techsystems Inc. Broadband patch antenna and antenna system
US8273694B2 (en) 2008-07-28 2012-09-25 Jeffrey A Brown Synthetic compositions obtained from algae
WO2010021711A1 (en) 2008-08-18 2010-02-25 Ls9, Inc. Systems and methods for the production of mixed fatty esters
TW201016657A (en) 2008-08-28 2010-05-01 Mitsui Chemicals Inc Olefin production process
JP2012504963A (ja) 2008-10-07 2012-03-01 エルエス９・インコーポレイテッド 脂肪アルデヒドを生産するための方法および組成物
CA3041892C (en) 2008-10-28 2022-03-15 REG Life Sciences, LLC Methods for producing a fatty alcohol in a host cell
US7989875B2 (en) 2008-11-24 2011-08-02 Nxp B.V. BiCMOS integration of multiple-times-programmable non-volatile memories
AP2011005777A0 (en) 2008-12-23 2011-08-31 Ls9 Inc Methods and compositions related to thioesterase enzymes.
US8071799B2 (en) 2009-01-29 2011-12-06 Energy & Environmental Research Center Foundation Chain-selective synthesis of fuel components and chemical feedstocks
CA2758298A1 (en) 2009-04-10 2010-10-14 Ls9, Inc. Production of commercial biodiesel from genetically modified microorganisms
EP2425007A4 (en) 2009-04-27 2013-01-02 Ls9 Inc PREPARATION OF FATTY ACID ESTERS
WO2010127318A2 (en) 2009-05-01 2010-11-04 The Regents Of The University Of California Product of fatty acid esters from biomass polymers
CN105969710A (zh) 2009-09-25 2016-09-28 Reg生命科学有限责任公司 脂肪酸衍生物的产生
US8237003B2 (en) 2009-11-09 2012-08-07 Exxonmobil Chemical Patents Inc. Metathesis catalyst and process for use thereof
US8809563B2 (en) 2009-11-09 2014-08-19 Exxonmobil Chemical Patents Inc. Metathesis catalyst and process for use thereof
US8361769B1 (en) 2009-11-16 2013-01-29 U.S. Department Of Energy Regioselective alkane hydroxylation with a mutant CYP153A6 enzyme
WO2011062987A2 (en) 2009-11-17 2011-05-26 Ls9, Inc. Methods and compositions for producing hydrocarbons
US8530221B2 (en) 2010-01-14 2013-09-10 Ls9, Inc. Production of branched chain fatty acids and derivatives thereof in recombinant microbial cells
US20110250663A1 (en) 2010-04-08 2011-10-13 Ls9, Inc. Methods and compositions related to fatty alcohol biosynthetic enzymes
CA2801057C (en) 2010-05-28 2019-06-18 Solazyme, Inc. Tailored oils produced from recombinant heterotrophic microorganisms
US8372610B2 (en) 2010-09-15 2013-02-12 Ls9, Inc. Production of odd chain fatty acid derivatives in recombinant microbial cells
WO2012071439A1 (en) 2010-11-22 2012-05-31 The Regents Of The University Of California Host cells and methods for producing diacid compounds
MX351063B (es) 2011-02-02 2017-09-29 Terravia Holdings Inc Aceites adaptados producidos a partir de microorganismos oleaginosos recombinantes.
CA2841796C (en) 2011-07-06 2021-06-29 Verdezyne, Inc. Biological methods for preparing a fatty dicarboxylic acid
DE102011110946A1 (de) 2011-08-15 2016-01-21 Evonik Degussa Gmbh Biotechnologisches Syntheseverfahren von omegafunktionalisierten Carbonsäuren und Carbonsäure-Estern aus einfachen Kohlenstoffquellen
EP2639308A1 (de) 2012-03-12 2013-09-18 Evonik Industries AG Enzymatische omega-Oxidation und -Aminierung von Fettsäuren
FR3003577B1 (fr) 2013-03-19 2016-05-06 Ferropem Inoculant a particules de surface
SI2984490T1 (sl) * 2013-06-14 2020-10-30 Genomatica, Inc. Metode proizvodnje derivatov omega hidroksilirane maščobne kisline
EP3087179B1 (en) 2014-06-16 2020-04-08 Genomatica, Inc. Omega-hydroxylase-related fusion polypeptides with improved properties
WO2017101987A1 (en) 2015-12-15 2017-06-22 REG Life Sciences, LLC Omega-hydroxylase-related fusion polypeptide variants with improved properties

Also Published As

Publication number Publication date
KR20220124292A (ko) 2022-09-13
AU2015277261A1 (en) 2017-02-02
ES2803560T3 (es) 2021-01-27
WO2015195697A1 (en) 2015-12-23
MY198268A (en) 2023-08-18
JP2017518060A (ja) 2017-07-06
AU2015277261B2 (en) 2021-02-18
EP3690035A2 (en) 2020-08-05
JP2022023237A (ja) 2022-02-07
PL3087179T3 (pl) 2020-09-21
BR112016029235B1 (pt) 2023-11-28
KR102663049B1 (ko) 2024-05-13
JP6826435B2 (ja) 2021-02-03
CA2951925A1 (en) 2015-12-23
JP2020036610A (ja) 2020-03-12
KR102439589B1 (ko) 2022-09-06
US11441130B2 (en) 2022-09-13
EP3087179B1 (en) 2020-04-08
KR20170066306A (ko) 2017-06-14
MX2016016565A (es) 2017-04-25
EP3690035A3 (en) 2020-10-21
WO2015195697A9 (en) 2016-02-25
CN114605558A (zh) 2022-06-10
CN106604990A (zh) 2017-04-26
US11421206B2 (en) 2022-08-23
CN106604990B (zh) 2022-04-05
AU2021202194A1 (en) 2021-05-06
US20230167419A1 (en) 2023-06-01
BR112016029235A2 (pt) 2018-07-10
EP3087179A1 (en) 2016-11-02
US20200370024A1 (en) 2020-11-26
JP7564800B2 (ja) 2024-10-09
US20170145390A1 (en) 2017-05-25

Similar Documents

Publication Publication Date Title
HK1250041A1 (zh) 多肽
IL258766B (en) Materials with improved properties
SG11201702796WA (en) Mutated immunoglobulin-binding polypeptides
HK1232136A1 (zh) 融合蛋白
HK1232234A1 (zh) 融合蛋白
IL253004B (en) il-17a binding polypeptides
HK1250040A1 (zh) 多肽
HRP20200170T1 (hr) Celobioza fosforilaza
ZA201701348B (en) New polypeptides
PL3087179T3 (pl) Polipeptydy fuzyjne związane z omega-hydroksylazą o ulepszonych właściwościach
SI3390620T1 (sl) Fuzijske variante polipeptida z izboljšanimi lastnostmi, povezane z omega-hidroksilazo
GB2532496B (en) Crystal-pair counter-rotator with translation capability
SG11201606501PA (en) Nanobody-fluorescent protein fusion
IL246533A0 (en) new proteins
GB201713537D0 (en) Fusion Polypeptide
GB201419237D0 (en) Novel polypeptides
GB201503789D0 (en) Fusion polypeptide
GB201403024D0 (en) Polypeptides
GB201410265D0 (en) Novel polypeptides
HU4466U (en) Bird-friendly cross-holder with fitting