SI1394173T1 - Izboljsan postopek za bisulfitno obdelavo - Google Patents

Izboljsan postopek za bisulfitno obdelavo

Info

Publication number
SI1394173T1
SI1394173T1 SI200331015T SI200331015T SI1394173T1 SI 1394173 T1 SI1394173 T1 SI 1394173T1 SI 200331015 T SI200331015 T SI 200331015T SI 200331015 T SI200331015 T SI 200331015T SI 1394173 T1 SI1394173 T1 SI 1394173T1
Authority
SI
Slovenia
Prior art keywords
solid phase
glass
nucleic acid
bisulfite
improved method
Prior art date
Application number
SI200331015T
Other languages
English (en)
Inventor
Christine Dr Markert-Hahn
Dirk Dr Block
Original Assignee
Hoffmann La Roche
Epigenomics Ag
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=32071078&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SI1394173(T1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Priority claimed from EP02019097A external-priority patent/EP1394172A1/en
Application filed by Hoffmann La Roche, Epigenomics Ag filed Critical Hoffmann La Roche
Publication of SI1394173T1 publication Critical patent/SI1394173T1/sl

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Compounds Of Iron (AREA)
  • Saccharide Compounds (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
SI200331015T 2002-08-29 2003-08-27 Izboljsan postopek za bisulfitno obdelavo SI1394173T1 (sl)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP02019097A EP1394172A1 (en) 2002-08-29 2002-08-29 Improved method for bisulfite treatment
EP02028114 2002-12-18
EP03019321A EP1394173B9 (en) 2002-08-29 2003-08-27 Improved method for bisulfite treatment

Publications (1)

Publication Number Publication Date
SI1394173T1 true SI1394173T1 (sl) 2008-02-29

Family

ID=32071078

Family Applications (2)

Application Number Title Priority Date Filing Date
SI200332443T SI1829886T1 (sl) 2002-08-29 2003-08-27 Izboljšan postopek za bisulfit obdelavo
SI200331015T SI1394173T1 (sl) 2002-08-29 2003-08-27 Izboljsan postopek za bisulfitno obdelavo

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SI200332443T SI1829886T1 (sl) 2002-08-29 2003-08-27 Izboljšan postopek za bisulfit obdelavo

Country Status (15)

Country Link
US (2) US9394332B2 (sl)
EP (2) EP1829886B1 (sl)
JP (2) JP5153048B2 (sl)
CN (1) CN1271081C (sl)
AT (1) ATE374782T1 (sl)
AU (1) AU2003236461B2 (sl)
CA (1) CA2438327C (sl)
CY (1) CY1107118T1 (sl)
DE (1) DE60316642T2 (sl)
DK (2) DK1394173T3 (sl)
ES (2) ES2545577T3 (sl)
HK (1) HK1062688A1 (sl)
HU (1) HUE027240T2 (sl)
PT (1) PT1394173E (sl)
SI (2) SI1829886T1 (sl)

Families Citing this family (27)

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DE10154317B4 (de) * 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
US9394332B2 (en) * 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
US7262013B2 (en) * 2003-08-29 2007-08-28 Applera Corporation Bisulfite method
US7368239B2 (en) * 2003-08-29 2008-05-06 Applera Corporation Method and materials for polyamine catalyzed bisulfite conversion of cytosine to uracil
US7534873B2 (en) * 2003-08-29 2009-05-19 Applied Biosystems, Llc Method and materials for quaternary amine catalyzed bisulfite conversion of cytosine to uracil
US7371526B2 (en) * 2003-08-29 2008-05-13 Applera Corporation Method and materials for bisulfite conversion of cytosine to uracil
CA2540310A1 (en) 2003-10-09 2005-04-28 Epigenomics Ag Improved bisulfite conversion of dna
US20080070240A2 (en) * 2004-04-08 2008-03-20 Toyo Boseki Kabushiki Kaisha Composition for deaminating dna and method of detecting methylated dna
EP1632578A1 (en) * 2004-09-03 2006-03-08 Roche Diagnostics GmbH DNA decontamination method
CA2581140A1 (en) * 2004-09-21 2006-03-30 Applera Corporation Methods of using sulfur nucleophiles as improved alternatives to sodium bisulfite for methylated dna analysis
US7658288B2 (en) 2004-11-08 2010-02-09 Applied Biosystems, Llc Bisulfite conversion reagent
EP2385136A1 (en) 2005-04-01 2011-11-09 Epigenomics AG Improved bisulfite conversion of DNA
EP1963352B1 (en) * 2005-12-14 2010-09-08 Roche Diagnostics GmbH New method for bisulfite treatment
US7820385B2 (en) * 2006-03-22 2010-10-26 The United States Of America As Represented By The Department Of Health And Human Services, Centers For Disease Control And Prevention Method for retaining methylation pattern in globally amplified DNA
US20080213870A1 (en) * 2007-03-01 2008-09-04 Sean Wuxiong Cao Methods for obtaining modified DNA from a biological specimen
WO2009088987A2 (en) * 2008-01-03 2009-07-16 The Johns Hopkins University Compositions and methods for polynucleotide extraction and methylation detection
US9115386B2 (en) 2008-09-26 2015-08-25 Children's Medical Center Corporation Selective oxidation of 5-methylcytosine by TET-family proteins
FR2940805B1 (fr) 2009-01-05 2015-10-16 Biomerieux Sa Procede d'amplification et/ou de detection d'acides nucleiques, kits et utilisations de ce procede
EP3141616B1 (en) 2011-07-29 2020-05-13 Cambridge Epigenetix Limited Methods for detection of nucleotide modification
ES2669512T3 (es) 2012-11-30 2018-05-28 Cambridge Epigenetix Limited Agente oxidante para nucleótidos modificados
EP3782731A1 (en) * 2015-06-10 2021-02-24 Biocartis N.V. Improved detection of methylated dna
US11603555B2 (en) 2015-06-15 2023-03-14 Cepheid Integrated purification and measurement of DNA methylation and co-measurement of mutations and/or MRNA expression levels in an automated reaction cartridge
MX2019006628A (es) 2016-12-12 2019-11-12 Cepheid Purificacion y medicion integradas de metilacion de adn y co-medicion de mutaciones y/o niveles de expresion de arnm en un cartucho de reaccion automatizado.
CN108265050A (zh) * 2018-03-13 2018-07-10 普迈德（北京）科技有限公司 一种血浆直接亚硫酸氢盐转化的方法及其应用
CA3111887A1 (en) 2018-09-27 2020-04-02 Grail, Inc. Methylation markers and targeted methylation probe panel
DE102019118332B4 (de) 2019-07-07 2022-04-07 Ist Innuscreen Gmbh Verfahren und testkit zur bisulfitmodifizierung von dna
CN114317761A (zh) * 2022-02-23 2022-04-12 厦门飞朔生物技术有限公司 一种宫颈癌相关基因甲基化检测方法

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US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
DK171161B1 (da) 1985-03-28 1996-07-08 Hoffmann La Roche Fremgangsmåde til påvisning af forekomst eller fravær af mindst én specifik nukleinsyresekvens i en prøve eller til skelnen mellem to forskellige nukleinsyresekvenser i denne prøve
DE3724442A1 (de) 1987-07-23 1989-02-02 Europ Lab Molekularbiolog Verfahren und vorrichtung zur reinigung von m-13-phagen-dna
IE61148B1 (en) 1988-03-10 1994-10-05 Ici Plc Method of detecting nucleotide sequences
US5130238A (en) 1988-06-24 1992-07-14 Cangene Corporation Enhanced nucleic acid amplification process
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US5786146A (en) 1996-06-03 1998-07-28 The Johns Hopkins University School Of Medicine Method of detection of methylated nucleic acid using agents which modify unmethylated cytosine and distinguishing modified methylated and non-methylated nucleic acids
NZ502323A (en) 1996-06-04 2001-09-28 Univ Utah Res Found Monitoring a fluorescence energy transfer pair during hybridization of first probe labelled with fluorescein to second probe labelled with Cy5 or Cy5.5
WO1998046797A1 (en) * 1997-04-16 1998-10-22 Immunological Associates Of Denver Nucleic acid archiving
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PT1144620E (pt) 1998-11-30 2004-01-30 Roche Diagnostics Gmbh Perticulas magneticas para a purificacao de acidos nucleicos
DE19856064C2 (de) 1998-12-04 2000-11-30 Invitek Gmbh Universelles Verfahren zur Isolierung von DNA aus beliebigen Ausgangsmaterialien
US6331393B1 (en) 1999-05-14 2001-12-18 University Of Southern California Process for high-throughput DNA methylation analysis
US6423536B1 (en) 1999-08-02 2002-07-23 Molecular Dynamics, Inc. Low volume chemical and biochemical reaction system
PT1232502E (pt) * 1999-11-17 2006-05-31 Roche Diagnostics Gmbh Particulas de vidro magneticas, metodo para a sua preparacao e suas utilizacoes
DE19958042A1 (de) 1999-12-03 2001-06-21 Invitek Gmbh Oberflächenmodifizierte Trägermaterialien zur Bindung biologischer Materialien, Verfahren zu ihrer Herstellung und ihre Verwendung
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DE10154317B4 (de) 2001-10-26 2005-06-09 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben
US9394332B2 (en) * 2002-08-29 2016-07-19 Epigenomics Ag Method for bisulfite treatment
EP1394172A1 (en) 2002-08-29 2004-03-03 Boehringer Mannheim Gmbh Improved method for bisulfite treatment
CA2547743C (en) * 2003-12-02 2011-06-28 F. Hoffmann-La Roche Ag Improved method for bisulfite treatment
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Also Published As

Publication number Publication date
EP1829886B1 (en) 2015-07-29
ES2545577T3 (es) 2015-09-14
EP1394173A1 (en) 2004-03-03
JP2004089195A (ja) 2004-03-25
CN1271081C (zh) 2006-08-23
DK1394173T3 (da) 2008-02-04
ES2294231T3 (es) 2008-04-01
CA2438327A1 (en) 2004-02-29
US20160068888A1 (en) 2016-03-10
HK1062688A1 (en) 2004-11-19
CA2438327C (en) 2008-04-15
SI1829886T1 (sl) 2015-12-31
US20040241704A1 (en) 2004-12-02
DE60316642D1 (de) 2007-11-15
AU2003236461B2 (en) 2009-05-28
EP1394173B1 (en) 2007-10-03
JP5310059B2 (ja) 2013-10-09
US9394332B2 (en) 2016-07-19
JP2009106304A (ja) 2009-05-21
ATE374782T1 (de) 2007-10-15
DK1829886T3 (en) 2015-08-17
PT1394173E (pt) 2007-11-28
CN1495192A (zh) 2004-05-12
EP1394173B9 (en) 2008-10-15
CY1107118T1 (el) 2012-10-24
HUE027240T2 (en) 2016-10-28
DE60316642T2 (de) 2008-01-31
AU2003236461A1 (en) 2004-03-18
US9868756B2 (en) 2018-01-16
JP5153048B2 (ja) 2013-02-27
EP1829886A2 (en) 2007-09-05
EP1829886A3 (en) 2008-10-15

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