SG184563A1 - Methods for measuring enzyme activity useful in determining cell viability in non-purified samples - Google Patents

Methods for measuring enzyme activity useful in determining cell viability in non-purified samples Download PDF

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Publication number
SG184563A1
SG184563A1 SG2012076097A SG2012076097A SG184563A1 SG 184563 A1 SG184563 A1 SG 184563A1 SG 2012076097 A SG2012076097 A SG 2012076097A SG 2012076097 A SG2012076097 A SG 2012076097A SG 184563 A1 SG184563 A1 SG 184563A1
Authority
SG
Singapore
Prior art keywords
sample
microorganism
polymerase
nucleic acid
enzyme
Prior art date
Application number
SG2012076097A
Other languages
English (en)
Inventor
Shawn Mark O'hara
Daniel R Zweitzig
Original Assignee
Zeus Scientific Inc
Shawn Mark O'hara
Daniel R Zweitzig
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=44799329&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=SG184563(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Zeus Scientific Inc, Shawn Mark O'hara, Daniel R Zweitzig filed Critical Zeus Scientific Inc
Publication of SG184563A1 publication Critical patent/SG184563A1/en

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    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/25Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving enzymes not classifiable in groups C12Q1/26 - C12Q1/66
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/34Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase
    • C12Q1/42Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving hydrolase involving phosphatase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Toxicology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG2012076097A 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples SG184563A1 (en)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US32493910P 2010-04-16 2010-04-16
US32494910P 2010-04-16 2010-04-16
US32541310P 2010-04-19 2010-04-19
PCT/US2011/032600 WO2011130584A2 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Publications (1)

Publication Number Publication Date
SG184563A1 true SG184563A1 (en) 2012-11-29

Family

ID=44799329

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201502900PA SG10201502900PA (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
SG2012076097A SG184563A1 (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Family Applications Before (1)

Application Number Title Priority Date Filing Date
SG10201502900PA SG10201502900PA (en) 2010-04-16 2011-04-15 Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Country Status (16)

Country Link
US (3) US20130196318A1 (enExample)
EP (3) EP3284831B1 (enExample)
JP (2) JP2013537399A (enExample)
KR (2) KR20130094714A (enExample)
CN (1) CN103328652B (enExample)
AP (1) AP2012006530A0 (enExample)
AU (2) AU2011239587B2 (enExample)
CA (1) CA2796676C (enExample)
EA (1) EA201291001A1 (enExample)
ES (3) ES2644258T5 (enExample)
MY (1) MY161567A (enExample)
NZ (2) NZ701866A (enExample)
PH (2) PH12012502065A1 (enExample)
SG (2) SG10201502900PA (enExample)
WO (1) WO2011130584A2 (enExample)
ZA (1) ZA201208522B (enExample)

Families Citing this family (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2011239587B2 (en) 2010-04-16 2016-03-10 Momentum Bioscience, Ltd. Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
AU2013206888A1 (en) 2012-01-05 2014-08-21 Zeus Scientific, Inc. Improved DNA polymerase activity assays and methods enabling detection of viable microbes
KR20150009539A (ko) * 2012-04-12 2015-01-26 제우스 사이언티픽, 아이엔씨. 어떤 중합효소 연장 활성의 민감한, 정량적 측정에 유용한 중합효소 활성을 측정하고 생육성 세포의 존재를 결정하는 방법
GB201412316D0 (en) 2014-07-10 2014-08-27 Momentum Bioscience Ltd Detecting the absence of micro-organisms
GB2528647A (en) * 2014-07-10 2016-02-03 Momentum Bioscience Ltd Detecting viable microorganisms
US9879328B2 (en) * 2014-11-21 2018-01-30 GeneWeave Biosciences, Inc. Mechanisms of antimicrobial susceptibility
US10662484B1 (en) 2016-03-24 2020-05-26 Bench in a Box, LLC Two universal duplex primer sets and a combined real-time PCR and high resolution melt analysis assay for the amplification of gram-positive and gram-negative bacteria
GB201805479D0 (en) * 2018-04-03 2018-05-16 Momentum Bioscience Ltd Microorganism separation and detection
GB201914538D0 (en) 2019-10-08 2019-11-20 Momentum Bioscience Ltd Microorganism capture from antimicrobial-containing solution
US20230112608A1 (en) 2021-10-13 2023-04-13 Disruptive Oil And Gas Technologies Corp Nanobubble dispersions generated in electrochemically activated solutions
CN114032285B (zh) * 2021-11-24 2022-05-24 北京擎科生物科技有限公司 PolyA聚合酶活性检测方法
WO2025122959A1 (en) * 2023-12-07 2025-06-12 California Institute Of Technology Methods for the translated alignment of transcriptomic data at single-cell resolution
GB202405229D0 (en) 2024-04-12 2024-05-29 Momentum Bioscience Ltd Microorganism enumeration

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JPS6054697A (ja) * 1983-09-05 1985-03-29 イザド−ラ ブロドスキイ 診断方法および装置
US4683195A (en) 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
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SE462497B (sv) 1988-11-30 1990-07-02 Clas Fredrik Runesson Kaelland Saett foer att aktivitetsbestaemma polymeraser
EP0385410B1 (en) * 1989-02-28 1996-10-02 Canon Kabushiki Kaisha Partially double-stranded oligonucleotide and method for forming oligonucleotide
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GB2303919B (en) 1994-07-13 1998-08-26 Secr Defence Assay of a microorganism by quantification of adenylate kinase involving magnesium ion and adenosine diphosphate
US6054278A (en) * 1997-05-05 2000-04-25 The Perkin-Elmer Corporation Ribosomal RNA gene polymorphism based microorganism identification
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AU2011239587B2 (en) 2010-04-16 2016-03-10 Momentum Bioscience, Ltd. Methods for measuring enzyme activity useful in determining cell viability in non-purified samples

Also Published As

Publication number Publication date
SG10201502900PA (en) 2015-06-29
US10876173B2 (en) 2020-12-29
EP2558600A2 (en) 2013-02-20
JP2017148067A (ja) 2017-08-31
PH12012502065A1 (en) 2019-07-10
EP3617320B1 (en) 2022-01-26
NZ720529A (en) 2017-01-27
CN103328652B (zh) 2016-12-28
CA2796676C (en) 2020-07-14
EP3284831A1 (en) 2018-02-21
MY161567A (en) 2017-04-28
US20170321287A1 (en) 2017-11-09
AU2016203878A1 (en) 2016-06-30
EP2558600B2 (en) 2020-06-17
KR20130094714A (ko) 2013-08-26
AU2011239587B2 (en) 2016-03-10
US20180230551A1 (en) 2018-08-16
PH12016500300A1 (en) 2019-02-04
EP2558600B1 (en) 2017-10-04
JP6603956B2 (ja) 2019-11-13
AP2012006530A0 (en) 2012-10-31
ZA201208522B (en) 2014-08-27
KR102068136B1 (ko) 2020-01-20
US10870894B2 (en) 2020-12-22
CN103328652A (zh) 2013-09-25
CA2796676A1 (en) 2011-10-20
NZ701866A (en) 2016-07-29
WO2011130584A3 (en) 2013-07-11
KR20180086526A (ko) 2018-07-31
EA201291001A1 (ru) 2013-09-30
AU2011239587A1 (en) 2012-11-08
ES2906061T3 (es) 2022-04-13
AP3635A (enExample) 2016-03-09
ES2739806T3 (es) 2020-02-04
ES2644258T3 (es) 2017-11-28
ES2644258T5 (es) 2021-02-02
WO2011130584A2 (en) 2011-10-20
US20130196318A1 (en) 2013-08-01
EP3284831B1 (en) 2019-07-24
JP2013537399A (ja) 2013-10-03
EP3617320A1 (en) 2020-03-04
EP2558600A4 (en) 2014-04-30

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