ES2147757T3 - Procedimiento para la deteccion de la transcriptasa en reverso. - Google Patents

Procedimiento para la deteccion de la transcriptasa en reverso.

Info

Publication number
ES2147757T3
ES2147757T3 ES93911715T ES93911715T ES2147757T3 ES 2147757 T3 ES2147757 T3 ES 2147757T3 ES 93911715 T ES93911715 T ES 93911715T ES 93911715 T ES93911715 T ES 93911715T ES 2147757 T3 ES2147757 T3 ES 2147757T3
Authority
ES
Spain
Prior art keywords
pct
procedure
detection
amplified
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
ES93911715T
Other languages
English (en)
Inventor
Jorg Schupbach
Jurg Boni
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Individual
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Individual filed Critical Individual
Application granted granted Critical
Publication of ES2147757T3 publication Critical patent/ES2147757T3/es
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6867—Replicase-based amplification, e.g. using Q-beta replicase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/682—Signal amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6862—Ligase chain reaction [LCR]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Electronic Switches (AREA)
  • Automatic Cycles, And Cycles In General (AREA)

Abstract

SE PROPONE UNA PROPUESTA RELATIVA A UN PROCESO ULTRASENSITIVO PARA LA DETECCION DE ACTIVIDAD DE TRANSCRIPTASA (RT) INVERSA EN TRES ETAPAS. EN LA PRIMERA ETAPA EL MATERIAL A SER EXAMINADO SE PROCESA OCUPANDO UNA PANTALLA EN UNA PRIMERA COMBINACION EN UNA MEZCLA DE REACCION, Y UTILIZANDO LA ACTIVIDAD RT EN EL MATERIAL PROCESADO A SER EXAMINADO PARA PREPARAR UN ACIDO NUCLEICO A SER MULTIPLICADO EN UN PROCESO DE AMPLIFICACION DE ACIDO NUCLEICO, DADO DE TAL FORMA QUE PUEDE SER PRODUCIDA UNA SUSTANCIA SOLAMENTE SI SE ENCUENTRA ACTIVIDAD RT EN EL MATERIAL A SER EXAMINADO. EN UNA SEGUNDA ETAPA, EL ACIDO NUCLEICO A SER INCREMENTADO SE AMPLIFICA PARA SUMINISTRAR UN PRODUCTO DE AMPLIFICACION. EN LA TERCERA ETAPA, EL PRODUCTO DE AMPLIFICACION SE ANALIZA Y SE IDENTIFICA. LA SENSITIVIDAD INCREMENTA DEL MODO COMO SE INCREMENTA CONTRA ASSAYS DE TIPO ANTERIOR DE RT Y DEL ORDEN DE VARIOS MILLONES, PARA FACILITAR DE ESTA FORMA LA DETECCION ULTRASENSITIVA DE LA ACTIVIDAD RT Y DE AQUI LA DETECCION DE TODOS LOSRETROVIRUS Y DE TODOS LOS OTROS RETROELEMENTOS QUE CONTIENEN O EXPRESAN RT ACTIVO. EL PROCESO ES IMPORTANTE EN LA DIAGNOSIS DE INFECCIONES Y ENFERMEDADES ORIGINADAS EN HUMANOS, ANIMALES Y PLANTAS. SE PROPONEN KITS DE APANTALLADO Y DE TIPIFICACION PARA EXAMEN Y DIAGNOSTICO.
ES93911715T 1992-05-11 1993-05-10 Procedimiento para la deteccion de la transcriptasa en reverso. Expired - Lifetime ES2147757T3 (es)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CH150292 1992-05-11

Publications (1)

Publication Number Publication Date
ES2147757T3 true ES2147757T3 (es) 2000-10-01

Family

ID=4211950

Family Applications (1)

Application Number Title Priority Date Filing Date
ES93911715T Expired - Lifetime ES2147757T3 (es) 1992-05-11 1993-05-10 Procedimiento para la deteccion de la transcriptasa en reverso.

Country Status (8)

Country Link
US (1) US5807669A (es)
EP (1) EP0623176B1 (es)
JP (1) JPH06509477A (es)
AT (1) ATE189003T1 (es)
AU (1) AU4258693A (es)
DE (1) DE59309936D1 (es)
ES (1) ES2147757T3 (es)
WO (1) WO1993023560A1 (es)

Families Citing this family (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6136535A (en) * 1991-11-14 2000-10-24 Digene Corporation Continuous amplification reaction
US5981179A (en) * 1991-11-14 1999-11-09 Digene Diagnostics, Inc. Continuous amplification reaction
JP4256931B2 (ja) * 1995-01-27 2009-04-22 アメリカ合衆国 逆転写酵素の高感度検出法
WO1999005527A2 (en) * 1997-07-22 1999-02-04 Novimmune Sa Methods for diagnosis and therapy of autoimmune disease, such as insulin dependent diabetes mellitus, involving retroviral superantigens
EP0893691A1 (en) * 1997-07-23 1999-01-27 Mach, Bernard François, Prof. Methods for diagnosis and therapy of autoimmunedisease, such as insulin dependent diabetes mellitus, involving retroviral superantigens
DE60018926T2 (de) * 1999-06-14 2006-04-13 Aventis Pasteur "cort"-pcr assay zur unterscheidung von endogener reversen transkriptase aktivität in eukaryontischen zellen von einer kontamination durch infektiöse retroviren
GB9923846D0 (en) * 1999-10-09 1999-12-08 Quip Technology Limited Reverse transcriptase assay
WO2009086353A1 (en) * 2007-12-26 2009-07-09 Helicos Biosciences Corporation Improved two-primer sequencing for high-throughput expression analysis
SG184563A1 (en) * 2010-04-16 2012-11-29 Zeus Scientific Inc Methods for measuring enzyme activity useful in determining cell viability in non-purified samples
US9777319B2 (en) * 2012-06-29 2017-10-03 General Electric Company Method for isothermal DNA amplification starting from an RNA template

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4965188A (en) * 1986-08-22 1990-10-23 Cetus Corporation Process for amplifying, detecting, and/or cloning nucleic acid sequences using a thermostable enzyme
EP0272098A3 (en) * 1986-12-15 1990-06-06 City Of Hope National Medical Center Method for amplification and detection of rna sequences
CA1340807C (en) * 1988-02-24 1999-11-02 Lawrence T. Malek Nucleic acid amplification process
US5021335A (en) * 1988-06-17 1991-06-04 The Board Of Trustees Of The Leland Stanford Junior University In situ transcription in cells and tissues
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
DE4027616A1 (de) * 1990-08-31 1992-03-05 Boehringer Mannheim Gmbh Verfahren zur bestimmung von polymerase-aktivitaet

Also Published As

Publication number Publication date
EP0623176B1 (de) 2000-01-19
US5807669A (en) 1998-09-15
WO1993023560A1 (de) 1993-11-25
DE59309936D1 (de) 2000-02-24
ATE189003T1 (de) 2000-02-15
JPH06509477A (ja) 1994-10-27
EP0623176A1 (de) 1994-11-09
AU4258693A (en) 1993-12-13

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