SG11202109469VA - Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest - Google Patents

Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest

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Publication number
SG11202109469VA
SG11202109469VA SG11202109469VA SG11202109469VA SG11202109469VA SG 11202109469V A SG11202109469V A SG 11202109469VA SG 11202109469V A SG11202109469V A SG 11202109469VA SG 11202109469V A SG11202109469V A SG 11202109469VA SG 11202109469V A SG11202109469V A SG 11202109469VA
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Singapore
Prior art keywords
gene
modifying
interest
introducing
rna molecules
Prior art date
Application number
SG11202109469VA
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English (en)
Inventor
Eyal Maori
Yaron Galanty
Cristina Pignocchi
Garcia Angela Chaparro
Ofir Meir
Original Assignee
Tropic Biosciences Uk Ltd
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Filing date
Publication date
Application filed by Tropic Biosciences Uk Ltd filed Critical Tropic Biosciences Uk Ltd
Publication of SG11202109469VA publication Critical patent/SG11202109469VA/en

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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C12N15/1131—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses
    • C12N15/1132—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing against viruses against retroviridae, e.g. HIV
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K31/00—Medicinal preparations containing organic active ingredients
    • A61K31/70—Carbohydrates; Sugars; Derivatives thereof
    • A61K31/7088—Compounds having three or more nucleosides or nucleotides
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • A61K38/16—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • A61K38/43—Enzymes; Proenzymes; Derivatives thereof
    • A61K38/46—Hydrolases (3)
    • A61K38/465—Hydrolases (3) acting on ester bonds (3.1), e.g. lipases, ribonucleases
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8218—Antisense, co-suppression, viral induced gene silencing [VIGS], post-transcriptional induced gene silencing [PTGS]
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    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
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    • C12N15/8283—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for virus resistance
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    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
    • C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
    • C12N15/8285—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for nematode resistance
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
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    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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  • Gastroenterology & Hepatology (AREA)
  • AIDS & HIV (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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SG11202109469VA 2019-03-14 2020-03-12 Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest SG11202109469VA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB1903519.5A GB201903519D0 (en) 2019-03-14 2019-03-14 Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest
PCT/IB2020/052248 WO2020183419A1 (en) 2019-03-14 2020-03-12 Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest

Publications (1)

Publication Number Publication Date
SG11202109469VA true SG11202109469VA (en) 2021-09-29

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SG11202109469VA SG11202109469VA (en) 2019-03-14 2020-03-12 Introducing silencing activity to dysfunctional rna molecules and modifying their specificity against a gene of interest

Country Status (12)

Country Link
US (1) US20220154187A1 (https=)
EP (1) EP3938512A1 (https=)
JP (2) JP2022524866A (https=)
KR (1) KR20210148187A (https=)
CN (1) CN113906138A (https=)
AU (1) AU2020237667B2 (https=)
BR (1) BR112021018159A2 (https=)
CA (1) CA3133198A1 (https=)
GB (1) GB201903519D0 (https=)
IL (1) IL286380A (https=)
SG (1) SG11202109469VA (https=)
WO (1) WO2020183419A1 (https=)

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SG11202002481RA (en) 2017-09-19 2020-04-29 Tropic Biosciences Uk Ltd Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants
AU2021353004A1 (en) 2020-09-30 2023-04-13 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
CN113430194B (zh) * 2020-11-30 2023-04-07 东北林业大学 一种基于CRISPR/Cas9的白桦基因编辑方法
GB202103256D0 (en) 2021-03-09 2021-04-21 Tropic Biosciences Uk Ltd Method for silencing genes
WO2022232054A1 (en) * 2021-04-26 2022-11-03 Gordian Biotechnology, Inc. Compositions and methods for in vivo screening of therapeutics
CN113502351B (zh) * 2021-06-29 2023-11-21 中国人民解放军疾病预防控制中心 一种crRNA及用于CRISPR-Cas12a检测人腺病毒核酸的方法
GB202112866D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to fusarium wilt in a banana
GB202112865D0 (en) 2021-09-09 2021-10-27 Tropic Biosciences Uk Ltd Resistance to black sigatoka disease in banana
CN115029378B (zh) * 2022-06-23 2023-09-15 河北北方学院 一种利用PtrDJ1C基因创制花斑观赏杨树的方法
CN115250913B (zh) * 2022-07-25 2023-06-20 三峡大学 一种盐肤木体细胞胚胎发生与植株再生方法
CN116218882A (zh) * 2022-10-11 2023-06-06 江苏省农业科学院 PI4KIIa在控制灰飞虱传播水稻黑条矮缩病毒中的应用
EP4649150A1 (en) 2023-01-13 2025-11-19 Laverock Therapeutics Limited Cell context-specific gene regulation using inhibitory rnas
GB202305021D0 (en) 2023-04-04 2023-05-17 Tropic Biosciences Uk Ltd Methods for generating breaks in a genome
CN116640196B (zh) * 2023-05-10 2024-02-06 山东农业大学 囊泡相关膜蛋白的相关蛋白vap1在抗马铃薯y病毒中的应用
CN120555500A (zh) * 2025-05-13 2025-08-29 中国农业大学 核糖核酸酶MdRNS3a在苹果综合抗性育种中的应用

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