SG11202011050TA - Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications - Google Patents
Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applicationsInfo
- Publication number
- SG11202011050TA SG11202011050TA SG11202011050TA SG11202011050TA SG11202011050TA SG 11202011050T A SG11202011050T A SG 11202011050TA SG 11202011050T A SG11202011050T A SG 11202011050TA SG 11202011050T A SG11202011050T A SG 11202011050TA SG 11202011050T A SG11202011050T A SG 11202011050TA
- Authority
- SG
- Singapore
- Prior art keywords
- reagents
- methods
- nucleic acid
- cell populations
- mixed cell
- Prior art date
Links
- 239000003153 chemical reaction reagent Substances 0.000 title 1
- 239000000203 mixture Substances 0.000 title 1
- 150000007523 nucleic acids Chemical class 0.000 title 1
- 102000039446 nucleic acids Human genes 0.000 title 1
- 108020004707 nucleic acids Proteins 0.000 title 1
Classifications
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
- G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
- G16B30/10—Sequence alignment; Homology search
-
- G—PHYSICS
- G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
- G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
- G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
- C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
- C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/16—Primer sets for multiplex assays
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/172—Haplotypes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Physics & Mathematics (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Analytical Chemistry (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Evolutionary Biology (AREA)
- Bioinformatics & Computational Biology (AREA)
- Theoretical Computer Science (AREA)
- Medical Informatics (AREA)
- Spectroscopy & Molecular Physics (AREA)
- Databases & Information Systems (AREA)
- Bioethics (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201862672573P | 2018-05-16 | 2018-05-16 | |
| US201962811517P | 2019-02-27 | 2019-02-27 | |
| PCT/US2019/032755 WO2019222560A1 (en) | 2018-05-16 | 2019-05-16 | Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11202011050TA true SG11202011050TA (en) | 2020-12-30 |
Family
ID=68540783
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11202011050TA SG11202011050TA (en) | 2018-05-16 | 2019-05-16 | Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications |
Country Status (10)
| Country | Link |
|---|---|
| US (2) | US20210292836A1 (https=) |
| EP (1) | EP3794120A4 (https=) |
| JP (2) | JP7497879B2 (https=) |
| KR (1) | KR102873073B1 (https=) |
| CN (1) | CN112218956A (https=) |
| AU (1) | AU2019269635A1 (https=) |
| BR (1) | BR112020023296A2 (https=) |
| CA (1) | CA3099819A1 (https=) |
| SG (1) | SG11202011050TA (https=) |
| WO (1) | WO2019222560A1 (https=) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US10844428B2 (en) | 2015-04-28 | 2020-11-24 | Illumina, Inc. | Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS) |
| CA3006792A1 (en) | 2015-12-08 | 2017-06-15 | Twinstrand Biosciences, Inc. | Improved adapters, methods, and compositions for duplex sequencing |
| SG11201906428SA (en) | 2017-01-18 | 2019-08-27 | Illumina Inc | Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths |
| AU2018261332B2 (en) | 2017-05-01 | 2024-12-05 | Illumina, Inc. | Optimal index sequences for multiplex massively parallel sequencing |
| EP3622089B1 (en) | 2017-05-08 | 2024-07-17 | Illumina, Inc. | Method for sequencing using universal short adapters for indexing of polynucleotide samples |
| US11447818B2 (en) | 2017-09-15 | 2022-09-20 | Illumina, Inc. | Universal short adapters with variable length non-random unique molecular identifiers |
| AU2018366213B2 (en) | 2017-11-08 | 2025-05-15 | Twinstrand Biosciences, Inc. | Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters |
| WO2019222560A1 (en) * | 2018-05-16 | 2019-11-21 | Twinstrand Biosciences, Inc. | Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications |
| BR112021000409A2 (pt) | 2018-07-12 | 2021-04-06 | Twinstrand Biosciences, Inc. | Métodos e reagentes para caracterizar edição genômica, expansão clonal e aplicações associadas |
| CN120877853A (zh) * | 2025-07-03 | 2025-10-31 | 武汉海希生物科技有限公司 | 一种基于二代测序数据检测嵌合率的方法和系统 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20120034603A1 (en) * | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
| US20130040375A1 (en) * | 2011-08-08 | 2013-02-14 | Tandem Diagnotics, Inc. | Assay systems for genetic analysis |
| PT2828218T (pt) * | 2012-03-20 | 2020-11-11 | Univ Washington Through Its Center For Commercialization | Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus |
| US10689706B2 (en) * | 2015-07-20 | 2020-06-23 | The Chinese University Of Hong Kong | Methylation pattern analysis of haplotypes in tissues in a DNA mixture |
| CA3006792A1 (en) * | 2015-12-08 | 2017-06-15 | Twinstrand Biosciences, Inc. | Improved adapters, methods, and compositions for duplex sequencing |
| US20190050530A1 (en) * | 2016-02-09 | 2019-02-14 | Toma Biosciences, Inc. | Systems and Methods for Analyzing Nucleic Acids |
| IL269431B2 (en) * | 2017-03-23 | 2025-05-01 | Univ Washington | Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing |
| CA3093846A1 (en) * | 2018-03-15 | 2019-09-19 | Twinstrand Biosciences, Inc. | Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations |
| WO2019222560A1 (en) * | 2018-05-16 | 2019-11-21 | Twinstrand Biosciences, Inc. | Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications |
| BR112021000409A2 (pt) * | 2018-07-12 | 2021-04-06 | Twinstrand Biosciences, Inc. | Métodos e reagentes para caracterizar edição genômica, expansão clonal e aplicações associadas |
-
2019
- 2019-05-16 WO PCT/US2019/032755 patent/WO2019222560A1/en not_active Ceased
- 2019-05-16 BR BR112020023296-3A patent/BR112020023296A2/pt unknown
- 2019-05-16 US US17/055,473 patent/US20210292836A1/en not_active Abandoned
- 2019-05-16 CA CA3099819A patent/CA3099819A1/en active Pending
- 2019-05-16 EP EP19804416.6A patent/EP3794120A4/en active Pending
- 2019-05-16 CN CN201980037564.3A patent/CN112218956A/zh active Pending
- 2019-05-16 KR KR1020207033828A patent/KR102873073B1/ko active Active
- 2019-05-16 AU AU2019269635A patent/AU2019269635A1/en active Pending
- 2019-05-16 SG SG11202011050TA patent/SG11202011050TA/en unknown
- 2019-05-16 JP JP2020564200A patent/JP7497879B2/ja active Active
-
2024
- 2024-05-23 JP JP2024083844A patent/JP2024116167A/ja active Pending
- 2024-07-31 US US18/790,637 patent/US20250232837A1/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| AU2019269635A1 (en) | 2020-11-26 |
| JP2024116167A (ja) | 2024-08-27 |
| JP7497879B2 (ja) | 2024-06-11 |
| JP2021524736A (ja) | 2021-09-16 |
| CA3099819A1 (en) | 2019-11-21 |
| BR112020023296A2 (pt) | 2021-05-04 |
| US20250232837A1 (en) | 2025-07-17 |
| EP3794120A4 (en) | 2022-03-23 |
| CN112218956A (zh) | 2021-01-12 |
| EP3794120A1 (en) | 2021-03-24 |
| WO2019222560A1 (en) | 2019-11-21 |
| KR20210013061A (ko) | 2021-02-03 |
| KR102873073B1 (ko) | 2025-10-17 |
| US20210292836A1 (en) | 2021-09-23 |
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