KR102873073B1 - 핵산 혼합물 및 혼합된 세포 집단을 해상하기 위한 방법 및 시약 및 관련 응용 - Google Patents

핵산 혼합물 및 혼합된 세포 집단을 해상하기 위한 방법 및 시약 및 관련 응용

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KR102873073B1
KR102873073B1 KR1020207033828A KR20207033828A KR102873073B1 KR 102873073 B1 KR102873073 B1 KR 102873073B1 KR 1020207033828 A KR1020207033828 A KR 1020207033828A KR 20207033828 A KR20207033828 A KR 20207033828A KR 102873073 B1 KR102873073 B1 KR 102873073B1
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nucleic acid
sequence
mixture
strand
sequencing
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KR20210013061A (ko
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제시 제이. 솔크
찰스 클린턴 3세 발렌타인
패트릭 다나허
팡 인 로
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트윈스트랜드 바이오사이언시스, 인코포레이티드
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    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
    • C—CHEMISTRY; METALLURGY
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/172—Haplotypes

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Analytical Chemistry (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Evolutionary Biology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Theoretical Computer Science (AREA)
  • Medical Informatics (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Databases & Information Systems (AREA)
  • Bioethics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
KR1020207033828A 2018-05-16 2019-05-16 핵산 혼합물 및 혼합된 세포 집단을 해상하기 위한 방법 및 시약 및 관련 응용 Active KR102873073B1 (ko)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US201862672573P 2018-05-16 2018-05-16
US62/672,573 2018-05-16
US201962811517P 2019-02-27 2019-02-27
US62/811,517 2019-02-27
PCT/US2019/032755 WO2019222560A1 (en) 2018-05-16 2019-05-16 Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications

Publications (2)

Publication Number Publication Date
KR20210013061A KR20210013061A (ko) 2021-02-03
KR102873073B1 true KR102873073B1 (ko) 2025-10-17

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KR1020207033828A Active KR102873073B1 (ko) 2018-05-16 2019-05-16 핵산 혼합물 및 혼합된 세포 집단을 해상하기 위한 방법 및 시약 및 관련 응용

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US (2) US20210292836A1 (https=)
EP (1) EP3794120A4 (https=)
JP (2) JP7497879B2 (https=)
KR (1) KR102873073B1 (https=)
CN (1) CN112218956A (https=)
AU (1) AU2019269635A1 (https=)
BR (1) BR112020023296A2 (https=)
CA (1) CA3099819A1 (https=)
SG (1) SG11202011050TA (https=)
WO (1) WO2019222560A1 (https=)

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US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
CA3006792A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
SG11201906428SA (en) 2017-01-18 2019-08-27 Illumina Inc Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
AU2018261332B2 (en) 2017-05-01 2024-12-05 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
EP3622089B1 (en) 2017-05-08 2024-07-17 Illumina, Inc. Method for sequencing using universal short adapters for indexing of polynucleotide samples
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
AU2018366213B2 (en) 2017-11-08 2025-05-15 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
WO2019222560A1 (en) * 2018-05-16 2019-11-21 Twinstrand Biosciences, Inc. Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications
BR112021000409A2 (pt) 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. Métodos e reagentes para caracterizar edição genômica, expansão clonal e aplicações associadas
CN120877853A (zh) * 2025-07-03 2025-10-31 武汉海希生物科技有限公司 一种基于二代测序数据检测嵌合率的方法和系统

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JP2013532494A (ja) * 2010-08-06 2013-08-19 アリオサ ダイアグノスティックス インコーポレイテッド 試料中の発生源寄与の決定のためのアッセイシステム

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US20130040375A1 (en) * 2011-08-08 2013-02-14 Tandem Diagnotics, Inc. Assay systems for genetic analysis
PT2828218T (pt) * 2012-03-20 2020-11-11 Univ Washington Through Its Center For Commercialization Métodos para baixar a taxa de erro da sequenciação paralela massiva de adn utilizando sequenciação duplex de consensus
US10689706B2 (en) * 2015-07-20 2020-06-23 The Chinese University Of Hong Kong Methylation pattern analysis of haplotypes in tissues in a DNA mixture
CA3006792A1 (en) * 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
US20190050530A1 (en) * 2016-02-09 2019-02-14 Toma Biosciences, Inc. Systems and Methods for Analyzing Nucleic Acids
IL269431B2 (en) * 2017-03-23 2025-05-01 Univ Washington Methods for targeted nucleic acid sequence enrichment with applications to error corrected nucleic acid sequencing
CA3093846A1 (en) * 2018-03-15 2019-09-19 Twinstrand Biosciences, Inc. Methods and reagents for enrichment of nucleic acid material for sequencing applications and other nucleic acid material interrogations
WO2019222560A1 (en) * 2018-05-16 2019-11-21 Twinstrand Biosciences, Inc. Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications
BR112021000409A2 (pt) * 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. Métodos e reagentes para caracterizar edição genômica, expansão clonal e aplicações associadas

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JP2013532494A (ja) * 2010-08-06 2013-08-19 アリオサ ダイアグノスティックス インコーポレイテッド 試料中の発生源寄与の決定のためのアッセイシステム

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Also Published As

Publication number Publication date
AU2019269635A1 (en) 2020-11-26
JP2024116167A (ja) 2024-08-27
JP7497879B2 (ja) 2024-06-11
JP2021524736A (ja) 2021-09-16
CA3099819A1 (en) 2019-11-21
BR112020023296A2 (pt) 2021-05-04
US20250232837A1 (en) 2025-07-17
EP3794120A4 (en) 2022-03-23
SG11202011050TA (en) 2020-12-30
CN112218956A (zh) 2021-01-12
EP3794120A1 (en) 2021-03-24
WO2019222560A1 (en) 2019-11-21
KR20210013061A (ko) 2021-02-03
US20210292836A1 (en) 2021-09-23

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