JP7497879B2 - 核酸混合物および混合細胞集団を解析するための方法および試薬ならびに関連用途 - Google Patents

核酸混合物および混合細胞集団を解析するための方法および試薬ならびに関連用途 Download PDF

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JP7497879B2
JP7497879B2 JP2020564200A JP2020564200A JP7497879B2 JP 7497879 B2 JP7497879 B2 JP 7497879B2 JP 2020564200 A JP2020564200 A JP 2020564200A JP 2020564200 A JP2020564200 A JP 2020564200A JP 7497879 B2 JP7497879 B2 JP 7497879B2
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mixture
nucleic acid
sequence
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sequencing
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JP2021524736A (ja
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ソーク，ジェシー・ジェイ
バレンタイン，チャールズ・クリントン・ザ・サード
ダナハー，パトリック
ロ，ファン・イン
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Twinstrand Biosciences Inc
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    • G16B20/00—ICT specially adapted for functional genomics or proteomics, e.g. genotype-phenotype associations
    • G16B20/20—Allele or variant detection, e.g. single nucleotide polymorphism [SNP] detection
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    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • G—PHYSICS
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    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B50/00—ICT programming tools or database systems specially adapted for bioinformatics
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    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/172—Haplotypes

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  • Chemical & Material Sciences (AREA)
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  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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JP2020564200A 2018-05-16 2019-05-16 核酸混合物および混合細胞集団を解析するための方法および試薬ならびに関連用途 Active JP7497879B2 (ja)

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JP2024083844A JP2024116167A (ja) 2018-05-16 2024-05-23 核酸混合物および混合細胞集団を解析するための方法および試薬ならびに関連用途

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US201862672573P 2018-05-16 2018-05-16
US62/672,573 2018-05-16
US201962811517P 2019-02-27 2019-02-27
US62/811,517 2019-02-27
PCT/US2019/032755 WO2019222560A1 (en) 2018-05-16 2019-05-16 Methods and reagents for resolving nucleic acid mixtures and mixed cell populations and associated applications

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JP2024083844A Pending JP2024116167A (ja) 2018-05-16 2024-05-23 核酸混合物および混合細胞集団を解析するための方法および試薬ならびに関連用途

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US (2) US20210292836A1 (https=)
EP (1) EP3794120A4 (https=)
JP (2) JP7497879B2 (https=)
KR (1) KR102873073B1 (https=)
CN (1) CN112218956A (https=)
AU (1) AU2019269635A1 (https=)
BR (1) BR112020023296A2 (https=)
CA (1) CA3099819A1 (https=)
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Cited By (1)

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JP2024116167A (ja) * 2018-05-16 2024-08-27 ツインストランド・バイオサイエンシズ・インコーポレイテッド 核酸混合物および混合細胞集団を解析するための方法および試薬ならびに関連用途

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US10844428B2 (en) 2015-04-28 2020-11-24 Illumina, Inc. Error suppression in sequenced DNA fragments using redundant reads with unique molecular indices (UMIS)
CA3006792A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
SG11201906428SA (en) 2017-01-18 2019-08-27 Illumina Inc Methods and systems for generation and error-correction of unique molecular index sets with heterogeneous molecular lengths
AU2018261332B2 (en) 2017-05-01 2024-12-05 Illumina, Inc. Optimal index sequences for multiplex massively parallel sequencing
EP3622089B1 (en) 2017-05-08 2024-07-17 Illumina, Inc. Method for sequencing using universal short adapters for indexing of polynucleotide samples
US11447818B2 (en) 2017-09-15 2022-09-20 Illumina, Inc. Universal short adapters with variable length non-random unique molecular identifiers
AU2018366213B2 (en) 2017-11-08 2025-05-15 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
BR112021000409A2 (pt) 2018-07-12 2021-04-06 Twinstrand Biosciences, Inc. Métodos e reagentes para caracterizar edição genômica, expansão clonal e aplicações associadas
CN120877853A (zh) * 2025-07-03 2025-10-31 武汉海希生物科技有限公司 一种基于二代测序数据检测嵌合率的方法和系统

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WO2013142389A1 (en) 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
WO2017100441A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
WO2017139492A1 (en) 2016-02-09 2017-08-17 Toma Biosciences, Inc. Systems and methods for analyzing nucelic acids

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US10689706B2 (en) * 2015-07-20 2020-06-23 The Chinese University Of Hong Kong Methylation pattern analysis of haplotypes in tissues in a DNA mixture
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WO2013142389A1 (en) 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
WO2017100441A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
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JP2024116167A (ja) * 2018-05-16 2024-08-27 ツインストランド・バイオサイエンシズ・インコーポレイテッド 核酸混合物および混合細胞集団を解析するための方法および試薬ならびに関連用途

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AU2019269635A1 (en) 2020-11-26
JP2024116167A (ja) 2024-08-27
JP2021524736A (ja) 2021-09-16
CA3099819A1 (en) 2019-11-21
BR112020023296A2 (pt) 2021-05-04
US20250232837A1 (en) 2025-07-17
EP3794120A4 (en) 2022-03-23
SG11202011050TA (en) 2020-12-30
CN112218956A (zh) 2021-01-12
EP3794120A1 (en) 2021-03-24
WO2019222560A1 (en) 2019-11-21
KR20210013061A (ko) 2021-02-03
KR102873073B1 (ko) 2025-10-17
US20210292836A1 (en) 2021-09-23

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