SG11201911869XA - Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells - Google Patents
Spatial indexing of genetic material and library preparation using hydrogel beads and flow cellsInfo
- Publication number
- SG11201911869XA SG11201911869XA SG11201911869XA SG11201911869XA SG11201911869XA SG 11201911869X A SG11201911869X A SG 11201911869XA SG 11201911869X A SG11201911869X A SG 11201911869XA SG 11201911869X A SG11201911869X A SG 11201911869XA SG 11201911869X A SG11201911869X A SG 11201911869XA
- Authority
- SG
- Singapore
- Prior art keywords
- genetic material
- flow cells
- library preparation
- hydrogel beads
- spatial indexing
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
- B01L3/502753—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by bulk separation arrangements on lab-on-a-chip devices, e.g. for filtration or centrifugation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6813—Hybridisation assays
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0652—Sorting or classification of particles or molecules
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
- B01L2200/06—Fluid handling related problems
- B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
- B01L2200/0668—Trapping microscopic beads
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/06—Auxiliary integrated devices, integrated components
- B01L2300/069—Absorbents; Gels to retain a fluid
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L2300/00—Additional constructional details
- B01L2300/08—Geometry, shape and general structure
- B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
- B01L2300/0877—Flow chambers
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
- C12Q2565/50—Detection characterised by immobilisation to a surface
- C12Q2565/514—Detection characterised by immobilisation to a surface characterised by the use of the arrayed oligonucleotides as identifier tags, e.g. universal addressable array, anti-tag or tag complement array
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Zoology (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Genetics & Genomics (AREA)
- Physics & Mathematics (AREA)
- General Engineering & Computer Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Medicinal Chemistry (AREA)
- Dispersion Chemistry (AREA)
- Hematology (AREA)
- Clinical Laboratory Science (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201762539949P | 2017-08-01 | 2017-08-01 | |
US201862663129P | 2018-04-26 | 2018-04-26 | |
PCT/US2018/044646 WO2019028047A1 (fr) | 2017-08-01 | 2018-07-31 | Indexation spatiale de matériel génétique et préparation de pharmacothèque à l'aide de billes d'hydrogel et de cellules d'écoulement |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201911869XA true SG11201911869XA (en) | 2020-01-30 |
Family
ID=65233034
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201911869XA SG11201911869XA (en) | 2017-08-01 | 2018-07-31 | Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells |
Country Status (5)
Country | Link |
---|---|
US (3) | US11352668B2 (fr) |
EP (2) | EP4289967A3 (fr) |
CN (1) | CN111108219A (fr) |
SG (1) | SG11201911869XA (fr) |
WO (1) | WO2019028047A1 (fr) |
Families Citing this family (33)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
SG11201908680YA (en) * | 2017-03-20 | 2019-10-30 | Illumina Inc | Methods and compositions for preparing nucleic acid libraries |
CA3186025A1 (fr) | 2018-02-13 | 2019-08-22 | Illumina, Inc. | Sequencage d'adn a l'aide de billes d'hydrogel |
CA3067181A1 (fr) | 2018-04-20 | 2019-10-24 | Illumina, Inc. | Procedes d'encapsulation de cellules uniques, cellules encapsulees et leurs utilisations |
US11142740B2 (en) | 2018-08-14 | 2021-10-12 | Inscripta, Inc. | Detection of nuclease edited sequences in automated modules and instruments |
TW202043486A (zh) | 2019-01-29 | 2020-12-01 | 美商伊路米納有限公司 | 定序套組 |
TW202045709A (zh) | 2019-01-29 | 2020-12-16 | 美商伊路米納有限公司 | 流體槽 |
US11347965B2 (en) | 2019-03-21 | 2022-05-31 | Illumina, Inc. | Training data generation for artificial intelligence-based sequencing |
US11210554B2 (en) | 2019-03-21 | 2021-12-28 | Illumina, Inc. | Artificial intelligence-based generation of sequencing metadata |
CN112888791A (zh) | 2019-04-29 | 2021-06-01 | 伊鲁米那股份有限公司 | 通过快速温育和核酸富集来鉴定和分析微生物样本 |
US11593649B2 (en) | 2019-05-16 | 2023-02-28 | Illumina, Inc. | Base calling using convolutions |
CA3149610A1 (fr) * | 2019-07-31 | 2021-02-04 | BioSkryb Genomics, Inc. | Analyse de cellules uniques |
NL2024527B1 (en) * | 2019-11-27 | 2021-08-30 | Illumina Inc | On-flow cell three-dimensional polymer structures |
MX2021010378A (es) * | 2019-11-27 | 2021-11-25 | Illumina Inc | Estructuras poliméricas tridimensionales en celda de flujo. |
NL2024596B1 (en) * | 2019-11-27 | 2021-08-30 | Illumina Inc | On-flow cell three-dimensional sequencing matrices |
NL2024528B1 (en) * | 2019-11-27 | 2021-08-30 | Illumina Inc | On-flow cell three-dimensional polymer structures having functionalized surfaces |
SG11202109771XA (en) * | 2019-12-11 | 2021-10-28 | Illumina Inc | Immobilization in flow cells |
EP4073245A1 (fr) * | 2019-12-12 | 2022-10-19 | Keygene N.V. | Manipulation d'acide nucléique semi-solide |
CA3157061A1 (fr) | 2020-01-27 | 2021-08-05 | Christian SILTANEN | Modules d'electroporation et instrumentation |
EP4107735A2 (fr) | 2020-02-20 | 2022-12-28 | Illumina, Inc. | Appel de base de plusieurs à plusieurs basé sur l'intelligence artificielle |
WO2021263008A1 (fr) * | 2020-06-26 | 2021-12-30 | The Regents Of The University Of California | Ajout sélectif de réactifs à des gouttelettes |
IL299042A (en) * | 2020-07-08 | 2023-02-01 | Illumina Inc | Beads as transpososome carriers |
EP4192951A1 (fr) | 2020-08-06 | 2023-06-14 | Illumina, Inc. | Préparation de banques de séquençage arn et adn à l'aide de transposomes liés à des billes |
AU2021380016A1 (en) * | 2020-11-16 | 2023-01-05 | Illumina Cambridge Limited | Altering flow cell signals |
KR20230142832A (ko) | 2021-01-08 | 2023-10-11 | 셀라노메, 인크. | 생물학적 샘플을 분석하기 위한 디바이스 및 방법 |
CA3206511A1 (fr) * | 2021-01-29 | 2022-08-04 | Yir-Shyuan WU | Procedes, compositions et kits pour ameliorer l'efficacite d'ensemencement de cellules d'ecoulement avec des polynucleotides |
US20220336054A1 (en) | 2021-04-15 | 2022-10-20 | Illumina, Inc. | Deep Convolutional Neural Networks to Predict Variant Pathogenicity using Three-Dimensional (3D) Protein Structures |
EP4352286A1 (fr) * | 2021-06-11 | 2024-04-17 | Cellanome, Inc. | Systèmes et méthodes d'analyse d'échantillons biologiques |
WO2023042153A1 (fr) * | 2021-09-17 | 2023-03-23 | Illumina Cambridge Limited | Compositions de réactif, procédés, cartouches et systèmes associés |
CA3223505A1 (fr) * | 2022-03-22 | 2023-09-28 | Zhong MEI | Reseau planaire chimique |
WO2023196603A1 (fr) * | 2022-04-08 | 2023-10-12 | Cellanome, Inc. | Codes-barres spatiaux par lithographie à l'hydrogel |
WO2023225366A1 (fr) * | 2022-05-20 | 2023-11-23 | Cellanome, Inc. | Procédé de construction d'une surface spatiallement codée |
US20240084359A1 (en) * | 2022-06-29 | 2024-03-14 | 10X Genomics, Inc. | Methods and compositions for patterned molecular array generation by directed bead delivery |
WO2024064639A1 (fr) * | 2022-09-19 | 2024-03-28 | Illumina, Inc. | Particules de nanogel ayant une double fonctionnalité et une réactivité à la température pour le regroupement de particules dans des systèmes de séquençage d'acide nucléique |
Family Cites Families (77)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US192A (en) | 1837-05-15 | Machine for cutting | ||
US8053A (en) | 1851-04-22 | Improvement in clogs or pattens | ||
US5130238A (en) | 1988-06-24 | 1992-07-14 | Cangene Corporation | Enhanced nucleic acid amplification process |
WO1991006678A1 (fr) | 1989-10-26 | 1991-05-16 | Sri International | Sequençage d'adn |
US5455166A (en) | 1991-01-31 | 1995-10-03 | Becton, Dickinson And Company | Strand displacement amplification |
US5677170A (en) | 1994-03-02 | 1997-10-14 | The Johns Hopkins University | In vitro transposition of artificial transposons |
KR100230718B1 (ko) | 1994-03-16 | 1999-11-15 | 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 | 등온 가닥 변위 핵산 증폭법 |
US5846719A (en) | 1994-10-13 | 1998-12-08 | Lynx Therapeutics, Inc. | Oligonucleotide tags for sorting and identification |
US5750341A (en) | 1995-04-17 | 1998-05-12 | Lynx Therapeutics, Inc. | DNA sequencing by parallel oligonucleotide extensions |
GB9620209D0 (en) | 1996-09-27 | 1996-11-13 | Cemu Bioteknik Ab | Method of sequencing DNA |
GB9626815D0 (en) | 1996-12-23 | 1997-02-12 | Cemu Bioteknik Ab | Method of sequencing DNA |
US7622294B2 (en) | 1997-03-14 | 2009-11-24 | Trustees Of Tufts College | Methods for detecting target analytes and enzymatic reactions |
ATE545710T1 (de) | 1997-04-01 | 2012-03-15 | Illumina Cambridge Ltd | Verfahren zur vervielfältigung von nukleinsäuren |
US6969488B2 (en) | 1998-05-22 | 2005-11-29 | Solexa, Inc. | System and apparatus for sequential processing of analytes |
AR021833A1 (es) | 1998-09-30 | 2002-08-07 | Applied Research Systems | Metodos de amplificacion y secuenciacion de acido nucleico |
US20050181440A1 (en) | 1999-04-20 | 2005-08-18 | Illumina, Inc. | Nucleic acid sequencing using microsphere arrays |
US6355431B1 (en) | 1999-04-20 | 2002-03-12 | Illumina, Inc. | Detection of nucleic acid amplification reactions using bead arrays |
US20060275782A1 (en) | 1999-04-20 | 2006-12-07 | Illumina, Inc. | Detection of nucleic acid reactions on bead arrays |
US6274320B1 (en) | 1999-09-16 | 2001-08-14 | Curagen Corporation | Method of sequencing a nucleic acid |
US7955794B2 (en) | 2000-09-21 | 2011-06-07 | Illumina, Inc. | Multiplex nucleic acid reactions |
US7582420B2 (en) | 2001-07-12 | 2009-09-01 | Illumina, Inc. | Multiplex nucleic acid reactions |
US6913884B2 (en) | 2001-08-16 | 2005-07-05 | Illumina, Inc. | Compositions and methods for repetitive use of genomic DNA |
US7611869B2 (en) | 2000-02-07 | 2009-11-03 | Illumina, Inc. | Multiplexed methylation detection methods |
DK1259643T3 (da) | 2000-02-07 | 2009-02-23 | Illumina Inc | Fremgangsmåde til detektion af nukleinsyre ved anvendelse af universel priming |
JP4954415B2 (ja) | 2000-02-10 | 2012-06-13 | イルミナ インコーポレイテッド | 試料のビーズをベースとした同時プロセシングのための基材としての個々のアレイのアレイおよびその製造法 |
US7001792B2 (en) | 2000-04-24 | 2006-02-21 | Eagle Research & Development, Llc | Ultra-fast nucleic acid sequencing device and a method for making and using the same |
EP2100971A3 (fr) | 2000-07-07 | 2009-11-25 | Visigen Biotechnologies, Inc. | Détermination de séquence en temps réel |
US7211414B2 (en) | 2000-12-01 | 2007-05-01 | Visigen Biotechnologies, Inc. | Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity |
US7057026B2 (en) | 2001-12-04 | 2006-06-06 | Solexa Limited | Labelled nucleotides |
US8030000B2 (en) | 2002-02-21 | 2011-10-04 | Alere San Diego, Inc. | Recombinase polymerase amplification |
EP3002289B1 (fr) | 2002-08-23 | 2018-02-28 | Illumina Cambridge Limited | Nucleotides modifies pour le sequençage de polynucleotide |
US7670810B2 (en) | 2003-06-20 | 2010-03-02 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
GB0321306D0 (en) | 2003-09-11 | 2003-10-15 | Solexa Ltd | Modified polymerases for improved incorporation of nucleotide analogues |
US20110059865A1 (en) | 2004-01-07 | 2011-03-10 | Mark Edward Brennan Smith | Modified Molecular Arrays |
GB2423819B (en) | 2004-09-17 | 2008-02-06 | Pacific Biosciences California | Apparatus and method for analysis of molecules |
WO2006064199A1 (fr) | 2004-12-13 | 2006-06-22 | Solexa Limited | Procede ameliore de detection de nucleotides |
EP1888743B1 (fr) | 2005-05-10 | 2011-08-03 | Illumina Cambridge Limited | Polymerases ameliorees |
GB0514936D0 (en) | 2005-07-20 | 2005-08-24 | Solexa Ltd | Preparation of templates for nucleic acid sequencing |
US7405281B2 (en) | 2005-09-29 | 2008-07-29 | Pacific Biosciences Of California, Inc. | Fluorescent nucleotide analogs and uses therefor |
EP4105644A3 (fr) | 2006-03-31 | 2022-12-28 | Illumina, Inc. | Systèmes et procédés pour analyse de séquençage par synthèse |
US8343746B2 (en) | 2006-10-23 | 2013-01-01 | Pacific Biosciences Of California, Inc. | Polymerase enzymes and reagents for enhanced nucleic acid sequencing |
EP2639578B1 (fr) | 2006-12-14 | 2016-09-14 | Life Technologies Corporation | Appareil de mesure d'analytes à l'aide de matrices de FET à grande échelle |
US8262900B2 (en) | 2006-12-14 | 2012-09-11 | Life Technologies Corporation | Methods and apparatus for measuring analytes using large scale FET arrays |
US8349167B2 (en) | 2006-12-14 | 2013-01-08 | Life Technologies Corporation | Methods and apparatus for detecting molecular interactions using FET arrays |
WO2008093098A2 (fr) | 2007-02-02 | 2008-08-07 | Illumina Cambridge Limited | Procedes pour indexer des echantillons et sequencer de multiples matrices nucleotidiques |
US8198028B2 (en) * | 2008-07-02 | 2012-06-12 | Illumina Cambridge Limited | Using populations of beads for the fabrication of arrays on surfaces |
US20100137143A1 (en) | 2008-10-22 | 2010-06-03 | Ion Torrent Systems Incorporated | Methods and apparatus for measuring analytes |
US9080211B2 (en) | 2008-10-24 | 2015-07-14 | Epicentre Technologies Corporation | Transposon end compositions and methods for modifying nucleic acids |
CA2804842A1 (fr) * | 2010-07-09 | 2012-01-12 | Board Of Regents Of The University Of Texas System | Echafaudages biodegradables |
US8829171B2 (en) | 2011-02-10 | 2014-09-09 | Illumina, Inc. | Linking sequence reads using paired code tags |
US9074251B2 (en) | 2011-02-10 | 2015-07-07 | Illumina, Inc. | Linking sequence reads using paired code tags |
AU2011323107B2 (en) | 2010-11-05 | 2015-09-10 | Illumina, Inc. | Linking sequence reads using paired code tags |
US8951781B2 (en) | 2011-01-10 | 2015-02-10 | Illumina, Inc. | Systems, methods, and apparatuses to image a sample for biological or chemical analysis |
EP2675819B1 (fr) * | 2011-02-18 | 2020-04-08 | Bio-Rad Laboratories, Inc. | Compositions et méthodes de marquage moléculaire |
CA2834291A1 (fr) | 2011-04-25 | 2012-11-01 | Biorad Laboratories, Inc. | Procedes et compositions pour l'analyse d'acide nucleique |
US8841071B2 (en) * | 2011-06-02 | 2014-09-23 | Raindance Technologies, Inc. | Sample multiplexing |
EP2718465B1 (fr) | 2011-06-09 | 2022-04-13 | Illumina, Inc. | Procede de fabrication d'un reseau d'analytes |
PT3290528T (pt) | 2011-09-23 | 2019-10-14 | Illumina Inc | Métodos e composições para sequenciamento de ácido nucleico |
WO2013063382A2 (fr) | 2011-10-28 | 2013-05-02 | Illumina, Inc. | Système et procédé de fabrication de micropuces |
EP3366348B1 (fr) | 2012-01-16 | 2023-08-23 | Greatbatch Ltd. | Traversée hermétique co-connectée filtrée emi, condensateur de traversée et ensemble de dérivation pour dispositif médical implantable actif |
US9176031B2 (en) | 2012-02-24 | 2015-11-03 | Raindance Technologies, Inc. | Labeling and sample preparation for sequencing |
IN2014DN07992A (fr) | 2012-04-03 | 2015-05-01 | Illumina Inc | |
US9012022B2 (en) | 2012-06-08 | 2015-04-21 | Illumina, Inc. | Polymer coatings |
US8895249B2 (en) | 2012-06-15 | 2014-11-25 | Illumina, Inc. | Kinetic exclusion amplification of nucleic acid libraries |
US9683230B2 (en) | 2013-01-09 | 2017-06-20 | Illumina Cambridge Limited | Sample preparation on a solid support |
US9512422B2 (en) | 2013-02-26 | 2016-12-06 | Illumina, Inc. | Gel patterned surfaces |
AU2013382098B2 (en) | 2013-03-13 | 2019-02-07 | Illumina, Inc. | Methods and compositions for nucleic acid sequencing |
US9555007B2 (en) * | 2013-03-14 | 2017-01-31 | Massachusetts Institute Of Technology | Multi-layer hydrogel capsules for encapsulation of cells and cell aggregates |
KR102436171B1 (ko) * | 2013-06-27 | 2022-08-24 | 10엑스 제노믹스, 인크. | 샘플 처리를 위한 조성물 및 방법 |
WO2015002813A1 (fr) | 2013-07-01 | 2015-01-08 | Illumina, Inc. | Greffage de polymère et fonctionnalisation de surface sans catalyseur |
CA2932283A1 (fr) * | 2013-12-20 | 2015-06-25 | Illumina, Inc. | Conservation des informations de connectivite genomique dans des echantillons d'adn genomiques fragmentes |
US9677132B2 (en) | 2014-01-16 | 2017-06-13 | Illumina, Inc. | Polynucleotide modification on solid support |
EP3632944B1 (fr) | 2014-10-31 | 2021-12-01 | Illumina Cambridge Limited | Polymères et revêtements de copolymères d'adn |
WO2016118915A1 (fr) * | 2015-01-22 | 2016-07-28 | Becton, Dickinson And Company | Dispositifs et systèmes permettant le codage moléculaire à barres d'acides nucléiques cibles dans des cellules individuelles |
CN107406890B (zh) * | 2015-02-10 | 2023-07-18 | 亿明达股份有限公司 | 用于分析细胞组分的方法和组合物 |
EP3957747A1 (fr) | 2015-07-27 | 2022-02-23 | Illumina, Inc. | Cartographie spatiale d'informations de séquence d'acide nucléique |
JP6824290B2 (ja) | 2016-05-18 | 2021-02-03 | イルミナ インコーポレイテッド | パターン化された疎水性表面を用いる自己組織化パターニング |
-
2018
- 2018-07-31 US US16/626,115 patent/US11352668B2/en active Active
- 2018-07-31 SG SG11201911869XA patent/SG11201911869XA/en unknown
- 2018-07-31 CN CN201880044550.XA patent/CN111108219A/zh active Pending
- 2018-07-31 EP EP23181622.4A patent/EP4289967A3/fr active Pending
- 2018-07-31 EP EP18841663.0A patent/EP3662083A4/fr active Pending
- 2018-07-31 WO PCT/US2018/044646 patent/WO2019028047A1/fr unknown
-
2022
- 2022-04-13 US US17/720,187 patent/US11649498B2/en active Active
-
2023
- 2023-03-08 US US18/180,622 patent/US20230220462A1/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20200216895A1 (en) | 2020-07-09 |
EP3662083A1 (fr) | 2020-06-10 |
EP4289967A3 (fr) | 2024-03-20 |
EP4289967A2 (fr) | 2023-12-13 |
US11649498B2 (en) | 2023-05-16 |
EP3662083A4 (fr) | 2021-03-03 |
US20230220462A1 (en) | 2023-07-13 |
US11352668B2 (en) | 2022-06-07 |
WO2019028047A1 (fr) | 2019-02-07 |
US20220243269A1 (en) | 2022-08-04 |
CN111108219A (zh) | 2020-05-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
SG11201911869XA (en) | Spatial indexing of genetic material and library preparation using hydrogel beads and flow cells | |
IL263920A (en) | Whole genome libraries for single cells and combinatorial indexing methods for them | |
HK1256303A1 (zh) | 程序性細胞死亡1配體1 (pd-l1)的irna組合物及其使用方法 | |
HK1250741A1 (zh) | 修飾的γδ細胞及其用途 | |
IL258397A (en) | Anti-aging antibodies and methods of using them | |
IL254734B1 (en) | Modified t cells and methods for their preparation and use | |
EP3553180A4 (fr) | Méthode de construction d'une banque de séquençage de cellule unique et son utilisation | |
EP3377523A4 (fr) | Récepteurs de cellules immunitaires répressibles sous conditions et leurs procédés d'utilisation | |
HK1256838A1 (zh) | 模塊化多肽文庫以及其製備和使用方法 | |
EP3278907A4 (fr) | Poudre métallique constituée de particules sphériques | |
EP3426772A4 (fr) | Cultures en suspension de cellules tumorales et procédés associés | |
SG11201705703QA (en) | Reduced volume electrochlorination cells and methods of manufacturing same | |
SG11201610126RA (en) | Systems and methods for synthetic biology design and host cell simulation | |
IL262251B1 (en) | New anti-syrap antibodies and their medical applications | |
EP3271154A4 (fr) | Filtrage de données de répartition spatiale de température de matériau de construction | |
HK1259296A1 (zh) | 基因組規模的t細胞活性陣列及其使用方法 | |
PL3510159T3 (pl) | Geny pestycydowe i sposoby stosowania | |
HK1253900A1 (zh) | 用於蛋白生產的改進的真核細胞及其製備方法 | |
IL252529A0 (en) | Stem cell material and methods of preparation | |
EP3039687A4 (fr) | Réacteurs à lit fluidisé comprenant des distributeurs coniques de gaz et procédés de fluoration associés | |
GB201705504D0 (en) | Methods of isolating naïve regulatory T cells | |
EP3325600A4 (fr) | Cuves modulaires à flux continu et procédés de séquençage | |
HK1256808A1 (zh) | 一種促細胞程序性壞死抗體及其應用 | |
EP3261758A4 (fr) | Catalyseurs d'argent ayant une taille et une densité de distribution améliorées des particules d'argent | |
ZA201902823B (en) | Stabilization of bodies of particulate material |