SG11201807073XA - Method for detecting an analyte using surface enhanced raman spectroscopy - Google Patents

Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property -, Organization 11M1111101110101011111 HO 11111 01110101111110110011101111111110111011# International Bureau 0.. .... .. ..... ..or::,„, (10) International Publication Number (43) International Publication Date WO 2017/146647 Al 31 August 2017 (31.08.2017) WIPO I PCT (51) International Patent Classification: GO1N 33/574 (2006.01) GO1N (21) International Application Number: (22) International Filing Date: 22 February 2017 (22.02.2017) (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/297,999 22 February 2016 (22.02.2016) (71) Applicant: AGENCY FOR SCIENCE, AND RESEARCH [SG/SG]; 1 Fusionopolis Connexis, Singapore 138632 (SG). (72) Inventors: OLIVO, Malini; c/o Consortium, Helios #01-02, 11 Biopolis 138667 (SG). PERUMAL, Jayakumar; Bioimaging Consortium, Helios #01-02, _ Singapore 138667 (SG). BALASUNDARAM, (81) Designated States (unless otherwise indicated, for every 21/65 (2006.01) kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, PCT/SG2017/050080 DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, English NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, English RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. US (84) Designated States (unless otherwise indicated, for every TECHNOLOGY kind of regional protection available): ARIPO (BW, GH, Way, #20-10 GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, Singapore Bioimaging TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, Way, Singapore DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, c/o Singapore SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, 11 Biopolis Way, GW, KM, ML, MR, NE, SN, TD, TG). Ghayathri; Helios #01-02, 11 Published: — with international search report (Art. 21(3)) & PARTNER LLP; Rochor Road, Singa- — c/o Singapore Bioimaging Consortium, _ Biopolis Way, Singapore 138667 (SG). — = (74) Agent: VIERING, JENTSCHURA P.O. Box 1088, Rochor Post Office, 911833 (SG). - pore = = = = Title: METHOD FOR DETECTING AN ANALYTE USING SURFACE ENHANCED RAMAN SPECTROSCOPY FIG. 15 — Cyst fluid with Hb + TM + H 2 0 2 + Au colloid - Only Cyst fluid + nos + H 2 0 2 + Au colloid (54) = = — = = = 30000 = tti tti c = — g 20000 — 0 ui _ c 10000 _ cu c 11 IN 71' iv _, (57) : According to the present L--. : (SERS) is provided. The method comprises (a) contacting one or more analyte-binding molecules with the analyte under conditions I L --- that allow binding of the analyte to the I-1 ably haptogloblin and the analyte-binding molecule may comprise haemoglobin or is a haptogloblin antibody, (b) contacting a liquid N reagent comprising a peroxidase substrate 0 pH of the second mixture at 10 or less, (c) quenching the second mixture to form a third mixture, (d) optionally contacting the third mixture with a SERS-active substrate, and (e) detecting a surface enhanced Raman signal from the third mixture and/or a surface of 5- the SERS-active substrate. 750 1000 1250 1500 1750 Wavenumber (cm') disclosure, a method for detecting an analyte using surface enhanced Raman spectroscopy one or more analyte-binding molecules to form a first mixture, wherein the analyte is prefer - and a peroxide source with the first mixture to form a second mixture, while maintaining