SG11201808775PA - Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free dna - Google Patents
Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free dnaInfo
- Publication number
- SG11201808775PA SG11201808775PA SG11201808775PA SG11201808775PA SG11201808775PA SG 11201808775P A SG11201808775P A SG 11201808775PA SG 11201808775P A SG11201808775P A SG 11201808775PA SG 11201808775P A SG11201808775P A SG 11201808775PA SG 11201808775P A SG11201808775P A SG 11201808775PA
- Authority
- SG
- Singapore
- Prior art keywords
- international
- sequencing
- california
- pct
- stanford
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
- C12Q1/6855—Ligating adaptors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
- C12Q1/6886—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material for cancer
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
- C12Q2525/10—Modifications characterised by
- C12Q2525/191—Modifications characterised by incorporating an adaptor
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2545/00—Reactions characterised by their quantitative nature
- C12Q2545/10—Reactions characterised by their quantitative nature the purpose being quantitative analysis
- C12Q2545/101—Reactions characterised by their quantitative nature the purpose being quantitative analysis with an internal standard/control
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2563/00—Nucleic acid detection characterized by the use of physical, structural and functional properties
- C12Q2563/185—Nucleic acid dedicated to use as a hidden marker/bar code, e.g. inclusion of nucleic acids to mark art objects or animals
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/154—Methylation markers
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B40/00—Libraries per se, e.g. arrays, mixtures
- C40B40/04—Libraries containing only organic compounds
- C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
- C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/04—Methods of creating libraries, e.g. combinatorial synthesis using dynamic combinatorial chemistry techniques
-
- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B70/00—Tags or labels specially adapted for combinatorial chemistry or libraries, e.g. fluorescent tags or bar codes
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Analytical Chemistry (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Pathology (AREA)
- Hospice & Palliative Care (AREA)
- Oncology (AREA)
- Biomedical Technology (AREA)
- Plant Pathology (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property —, Organization 111111110111101110101011111010111110111010111111101101111101111101111011111 International Bureau ... ..... ..Yi j ..... ..... (10) International Publication Number (43) International Publication Date WO 2017/176630 Al 12 October 2017(12.10.2017) WIPO I PCT (51) International Patent Classification: Office of the General Counsel, Building 170, 3rd Floor, C12N 15/00 (2006.01) C12Q 1/68 (2006.01) Main Quad, P.O. Box 20386, Stanford, California 94305- C12N 15/07 (2006.01) G01N 33/50 (2006.01) 2038 (US). (21) International Application Number: (72) Inventor; and PCT/US2017/025735 (71) Applicant (for US only): QUAKE, Stephen R. [US/US]; 318 Campus Drive, Stanford, California 94305 (US). (22) International Filing Date: 3 April 2017 (03.04.2017) (72) Inventor: SONG, Chunxiao; Ludwig Institute, University of Oxford, Old Road Campus Research Building, Heading- (25) Filing Language: English ton, Oxford OX3 7DQ (GB). (26) Publication Language: English (74) Agent: KEDDIE, James S.; 201 Redwood Shores Park- (30) Priority Data: way, Suite 200, Redwood City, California 94065 (US). 62/319,702 7 April 2016 (07.04.2016) US (81) Designated States (unless otherwise indicated, for every 62/444,122 9 January 2017 (09.01.2017) US kind of national protection available): AE, AG, AL, AM, 62/461,712 21 February 2017 (21.02.2017) US AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, (71) Applicant: THE BOARD OF TRUSTEES OF THE LE- BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, LAND STANFORD JUNIOR UNIVERSITY [US/US]; DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, [Continued on next page] = (54) Title: NONINVASIVE DIAGNOSTICS BY SEQUENCING 5-HYDROXYMETHYLATED CELL-FREE DNA (57) : Provided herein is a method of sequencing hydroxy- - _ ciDNA methyated cell-free DNA. In some embodiments, the method com- tag to hydroxymethyated DNA _ prises adding an affinity only mo- - a 5h mc 9 _.2____ lecules in a sample of cfDNA, enriching for the DNA molecules that are tagged with the affinity tag; and sequencing the enriched end repair &A-tailing DNA molecules. adapter ligation -,__ illurnina \" — adapter , -/' • 9 J , — ¨ , , , selective labeling of 5hmC with biotin , \----e-------, / • El biotin • \ . \ • _ • • — / I capture 5hmC + extensive wash M 1-1 strepdavidin beads • q • PCR from beads ir 0 final libary M IN FIG, 1 A ,—, IN ,—, © ei O WO 2017/176630 Al MUNRO 111111111111111111111111113111111111111111 Ell 0111110 111110111111111111 HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KH, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Declarations under Rule 4.17: TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. — of inventorship (Rule 4.17(iv)) (84) Designated States (unless otherwise indicated, for every Published: kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, — with international search report (Art. 21(3)) with sequence listing part of description (Rule 5.2(a)) TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE,
Applications Claiming Priority (4)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US201662319702P | 2016-04-07 | 2016-04-07 | |
US201762444122P | 2017-01-09 | 2017-01-09 | |
US201762461712P | 2017-02-21 | 2017-02-21 | |
PCT/US2017/025735 WO2017176630A1 (en) | 2016-04-07 | 2017-04-03 | Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free dna |
Publications (1)
Publication Number | Publication Date |
---|---|
SG11201808775PA true SG11201808775PA (en) | 2018-11-29 |
Family
ID=60000634
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
SG11201808775PA SG11201808775PA (en) | 2016-04-07 | 2017-04-03 | Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free dna |
Country Status (14)
Country | Link |
---|---|
US (7) | US10718010B2 (en) |
EP (2) | EP3440205B1 (en) |
JP (2) | JP7143221B2 (en) |
CN (1) | CN109312399B (en) |
AU (1) | AU2017246318B2 (en) |
CA (1) | CA3019836A1 (en) |
DK (1) | DK3440205T3 (en) |
ES (1) | ES2882329T3 (en) |
MX (1) | MX2018012156A (en) |
PL (1) | PL3440205T3 (en) |
PT (1) | PT3440205T (en) |
RU (1) | RU2742355C2 (en) |
SG (1) | SG11201808775PA (en) |
WO (1) | WO2017176630A1 (en) |
Families Citing this family (27)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US9115386B2 (en) | 2008-09-26 | 2015-08-25 | Children's Medical Center Corporation | Selective oxidation of 5-methylcytosine by TET-family proteins |
EP3425065B1 (en) | 2011-12-13 | 2021-04-21 | Oslo Universitetssykehus HF | Methods and kits for detection of methylation status |
ES2786984T3 (en) | 2012-11-30 | 2020-10-14 | Cambridge Epigenetix Ltd | Oxidizing agent for modified nucleotides |
WO2014191981A1 (en) | 2013-05-28 | 2014-12-04 | Ramot At Tel-Aviv University Ltd. | Detection of hydroxymethylcytosine bases |
WO2017059245A2 (en) | 2015-09-30 | 2017-04-06 | Trustees Of Boston University | Deadman and passcode microbial kill switches |
JP2021500883A (en) | 2017-10-27 | 2021-01-14 | ジュノ ダイアグノスティックス,インク. | Devices, systems, and methods for ultratrace liquid biopsy |
KR102503884B1 (en) | 2018-01-08 | 2023-03-07 | 루드비히 인스티튜트 포 캔서 리서치 리미티드 | Bisulfite-Free Base-Resolved Identification of Cytosine Modifications |
US11634748B2 (en) | 2018-02-14 | 2023-04-25 | Clearnote Health, Inc. | Methods for the epigenetic analysis of DNA, particularly cell-free DNA |
EP3762513A1 (en) | 2018-03-08 | 2021-01-13 | St. Johns University | Circulating serum cell-free dna biomarkers and methods |
SG11202012585YA (en) * | 2018-06-22 | 2021-01-28 | Bluestar Genomics Inc | Hydroxymethylation analysis of cell-free nucleic acid samples for assigning tissue of origin, and related methods of use |
CA3112880A1 (en) | 2018-09-19 | 2020-03-26 | Bluestar Genomics, Inc. | Cell-free dna hydroxymethylation profiles in the evaluation of pancreatic lesions |
EP3856903A4 (en) | 2018-09-27 | 2022-07-27 | Grail, LLC | Methylation markers and targeted methylation probe panel |
JP2022504078A (en) | 2018-10-04 | 2022-01-13 | ブルースター ジェノミクス, インコーポレイテッド | Sequencing-based simultaneous analysis of proteins, nucleosomes and cell-free nucleic acids from a single biological sample |
CN112867495A (en) * | 2018-10-19 | 2021-05-28 | 韩国生命工学研究院 | Gastric cancer therapeutic composition comprising SYT11 inhibitor as active ingredient |
CN109321647A (en) * | 2018-10-26 | 2019-02-12 | 苏州森苗生物科技有限公司 | The construction method of marking composition and methylolation nucleic acid library |
WO2020194057A1 (en) * | 2019-03-22 | 2020-10-01 | Cambridge Epigenetix Limited | Biomarkers for disease detection |
WO2020198312A1 (en) * | 2019-03-27 | 2020-10-01 | Juno Diagnostics, Inc. | Optimized ultra-low volume liquid biopsy methods, systems, and devices |
EP3990548A4 (en) * | 2019-06-25 | 2023-07-26 | AccuraGen Holdings Limited | Methods and systems for disease detection |
US11475981B2 (en) | 2020-02-18 | 2022-10-18 | Tempus Labs, Inc. | Methods and systems for dynamic variant thresholding in a liquid biopsy assay |
US11211147B2 (en) | 2020-02-18 | 2021-12-28 | Tempus Labs, Inc. | Estimation of circulating tumor fraction using off-target reads of targeted-panel sequencing |
US11211144B2 (en) | 2020-02-18 | 2021-12-28 | Tempus Labs, Inc. | Methods and systems for refining copy number variation in a liquid biopsy assay |
WO2021198726A1 (en) * | 2020-03-30 | 2021-10-07 | Vilnius University | Methods and compositions for noninvasive prenatal diagnosis through targeted covalent labeling of genomic sites |
AU2021319150A1 (en) | 2020-07-30 | 2023-03-02 | Cambridge Epigenetix Limited | Compositions and methods for nucleic acid analysis |
EP4200445A1 (en) | 2020-08-19 | 2023-06-28 | Mayo Foundation for Medical Education and Research | Detecting non-hodgkin lymphoma |
KR20230150814A (en) | 2021-01-29 | 2023-10-31 | 메이오 파운데이션 포 메디칼 에쥬케이션 앤드 리써치 | Detection of presence or absence of various types of cancer |
CN113528616A (en) * | 2021-07-26 | 2021-10-22 | 深圳泰莱生物科技有限公司 | Detection method for capturing cfDNA5hmC fragment |
CN115992203A (en) * | 2022-07-26 | 2023-04-21 | 生工生物工程(上海)股份有限公司 | Method for constructing genome-wide hydroxymethylation capture sequencing library |
Family Cites Families (35)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5712126A (en) | 1995-08-01 | 1998-01-27 | Yale University | Analysis of gene expression by display of 3-end restriction fragments of CDNA |
US5948902A (en) | 1997-11-20 | 1999-09-07 | South Alabama Medical Science Foundation | Antisense oligonucleotides to human serine/threonine protein phosphatase genes |
US6287825B1 (en) | 1998-09-18 | 2001-09-11 | Molecular Staging Inc. | Methods for reducing the complexity of DNA sequences |
US20050233340A1 (en) | 2004-04-20 | 2005-10-20 | Barrett Michael T | Methods and compositions for assessing CpG methylation |
US20100273151A1 (en) * | 2004-05-28 | 2010-10-28 | Fred Hutchinson Cancer Research Center | Genome-wide analysis of palindrome formation and dna methylation |
JP5904569B2 (en) * | 2005-06-08 | 2016-04-13 | ミレニアム ファーマシューティカルズ, インコーポレイテッドMillennium Pharmaceuticals, Inc. | Methods for identifying, determining and treating patients undergoing cancer therapy |
US7537897B2 (en) | 2006-01-23 | 2009-05-26 | Population Genetics Technologies, Ltd. | Molecular counting |
WO2008150432A1 (en) | 2007-06-01 | 2008-12-11 | 454 Life Sciences Corporation | System and meth0d for identification of individual samples from a multiplex mixture |
WO2009032167A1 (en) | 2007-08-29 | 2009-03-12 | Illumina Cambridge | Method for sequencing a polynucleotide template |
US9115386B2 (en) | 2008-09-26 | 2015-08-25 | Children's Medical Center Corporation | Selective oxidation of 5-methylcytosine by TET-family proteins |
US8541207B2 (en) * | 2008-10-22 | 2013-09-24 | Illumina, Inc. | Preservation of information related to genomic DNA methylation |
US20100323348A1 (en) | 2009-01-31 | 2010-12-23 | The Regents Of The University Of Colorado, A Body Corporate | Methods and Compositions for Using Error-Detecting and/or Error-Correcting Barcodes in Nucleic Acid Amplification Process |
CA2757300C (en) * | 2009-04-01 | 2018-01-09 | Dxterity Diagnostics Incorporated | Chemical ligation dependent probe amplification (clpa) |
WO2011025819A1 (en) * | 2009-08-25 | 2011-03-03 | New England Biolabs, Inc. | Detection and quantification of hydroxymethylated nucleotides in a polynucleotide preparation |
US8835358B2 (en) | 2009-12-15 | 2014-09-16 | Cellular Research, Inc. | Digital counting of individual molecules by stochastic attachment of diverse labels |
WO2011091146A1 (en) * | 2010-01-20 | 2011-07-28 | New England Biolabs, Inc. | Compositions, methods and related uses for cleaving modified dna |
WO2011127136A1 (en) | 2010-04-06 | 2011-10-13 | University Of Chicago | Composition and methods related to modification of 5-hydroxymethylcytosine (5-hmc) |
US20120034603A1 (en) * | 2010-08-06 | 2012-02-09 | Tandem Diagnostics, Inc. | Ligation-based detection of genetic variants |
ES2690753T3 (en) | 2010-09-21 | 2018-11-22 | Agilent Technologies, Inc. | Increased confidence in allele identifications with molecular count |
US20120122087A1 (en) | 2010-11-17 | 2012-05-17 | Weiwei Li | 5-Hydroxymethylcytosine as a biomarker for early detection, treatment and prognostic monitoring of cancer |
EP2694686B2 (en) | 2011-04-06 | 2023-07-19 | The University of Chicago | COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC) |
CN103748236B (en) | 2011-04-15 | 2018-12-25 | 约翰·霍普金斯大学 | Safe sequencing system |
ES2665071T3 (en) | 2012-02-17 | 2018-04-24 | Fred Hutchinson Cancer Research Center | Compositions and methods to identify mutations accurately |
WO2013142389A1 (en) | 2012-03-20 | 2013-09-26 | University Of Washington Through Its Center For Commercialization | Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing |
US9732390B2 (en) * | 2012-09-20 | 2017-08-15 | The Chinese University Of Hong Kong | Non-invasive determination of methylome of fetus or tumor from plasma |
CA2949622C (en) * | 2012-11-26 | 2019-07-02 | The University Of Toledo | Methods for standardized sequencing of nucleic acids and uses thereof |
EP2971100A1 (en) * | 2013-03-13 | 2016-01-20 | Sequenom, Inc. | Primers for dna methylation analysis |
US20160194696A1 (en) * | 2013-08-09 | 2016-07-07 | New England Biolabs, Inc. | Detecting, Sequencing and/or Mapping 5-Hydroxymethylcytosine and 5-Formylcytosine at Single-Base Resolution |
US20150050697A1 (en) * | 2013-08-19 | 2015-02-19 | Abbott Molecular Inc. | Nucleotide analogs |
JP6895753B2 (en) | 2014-01-07 | 2021-06-30 | フンダシオ プリバーダ インスティトゥト デ メディシナ プレディクティヴァ イ パーソナリトザダ デル キャンサー | Double-stranded DNA library production method and sequencing method for identification of methylated cytosine |
EP3099822A4 (en) * | 2014-01-30 | 2017-08-30 | The Regents of the University of California | Methylation haplotyping for non-invasive diagnosis (monod) |
GB201405226D0 (en) * | 2014-03-24 | 2014-05-07 | Cambridge Entpr Ltd | Nucleic acid preparation method |
WO2015188192A2 (en) * | 2014-06-06 | 2015-12-10 | Cornell University | Method for identification and enumeration of nucleic acid sequence, expression, copy, or dna methylation changes, using combined nuclease, ligase, polymerase, and sequencing reactions |
US20180046754A1 (en) * | 2015-03-24 | 2018-02-15 | Agency For Science, Technology And Research (A*Star) | Normalization methods for measuring gene copy number and expression |
US11162139B2 (en) | 2016-03-02 | 2021-11-02 | Shanghai Epican Genetech Co. Ltd. | Method for genomic profiling of DNA 5-methylcytosine and 5-hydroxymethylcytosine |
-
2017
- 2017-04-03 PL PL17779593T patent/PL3440205T3/en unknown
- 2017-04-03 WO PCT/US2017/025735 patent/WO2017176630A1/en active Application Filing
- 2017-04-03 EP EP17779593.7A patent/EP3440205B1/en active Active
- 2017-04-03 EP EP21167021.1A patent/EP3929290A1/en active Pending
- 2017-04-03 CA CA3019836A patent/CA3019836A1/en active Pending
- 2017-04-03 MX MX2018012156A patent/MX2018012156A/en unknown
- 2017-04-03 ES ES17779593T patent/ES2882329T3/en active Active
- 2017-04-03 JP JP2018553116A patent/JP7143221B2/en active Active
- 2017-04-03 CN CN201780031390.0A patent/CN109312399B/en active Active
- 2017-04-03 RU RU2018138848A patent/RU2742355C2/en active
- 2017-04-03 SG SG11201808775PA patent/SG11201808775PA/en unknown
- 2017-04-03 PT PT177795937T patent/PT3440205T/en unknown
- 2017-04-03 AU AU2017246318A patent/AU2017246318B2/en active Active
- 2017-04-03 DK DK17779593.7T patent/DK3440205T3/en active
-
2018
- 2018-09-28 US US16/146,807 patent/US10718010B2/en active Active
-
2020
- 2020-04-14 US US16/848,482 patent/US20200283838A1/en active Pending
- 2020-04-14 US US16/848,515 patent/US20200248249A1/en not_active Abandoned
- 2020-04-14 US US16/848,498 patent/US20200248248A1/en not_active Abandoned
- 2020-04-15 US US16/848,989 patent/US20200277666A1/en active Pending
- 2020-04-15 US US16/849,071 patent/US20200299760A1/en not_active Abandoned
- 2020-04-15 US US16/849,067 patent/US20200277667A1/en not_active Abandoned
-
2022
- 2022-06-07 JP JP2022092184A patent/JP2022120007A/en active Pending
Also Published As
Publication number | Publication date |
---|---|
US20190017109A1 (en) | 2019-01-17 |
PT3440205T (en) | 2021-08-06 |
AU2017246318A1 (en) | 2018-11-08 |
DK3440205T3 (en) | 2021-08-16 |
CA3019836A1 (en) | 2017-10-12 |
AU2017246318B2 (en) | 2023-07-27 |
EP3440205A1 (en) | 2019-02-13 |
US20200277666A1 (en) | 2020-09-03 |
RU2018138848A3 (en) | 2020-12-15 |
US20200299760A1 (en) | 2020-09-24 |
RU2018138848A (en) | 2020-05-12 |
EP3440205B1 (en) | 2021-05-26 |
US20200283838A1 (en) | 2020-09-10 |
PL3440205T3 (en) | 2021-11-22 |
ES2882329T3 (en) | 2021-12-01 |
CN109312399B (en) | 2023-02-03 |
JP7143221B2 (en) | 2022-09-28 |
US20200248249A1 (en) | 2020-08-06 |
EP3929290A1 (en) | 2021-12-29 |
CN109312399A (en) | 2019-02-05 |
JP2022120007A (en) | 2022-08-17 |
AU2017246318A2 (en) | 2018-11-08 |
EP3440205A4 (en) | 2019-04-03 |
MX2018012156A (en) | 2019-02-07 |
US20200248248A1 (en) | 2020-08-06 |
JP2019520791A (en) | 2019-07-25 |
US20200277667A1 (en) | 2020-09-03 |
US10718010B2 (en) | 2020-07-21 |
WO2017176630A1 (en) | 2017-10-12 |
RU2742355C2 (en) | 2021-02-05 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
SG11201808775PA (en) | Noninvasive diagnostics by sequencing 5-hydroxymethylated cell-free dna | |
SG11201804807VA (en) | Computer architecture and method for modifying data intake parameters based on a predictive model | |
SG11201803703UA (en) | Anti-complement factor c1q fab fragments and uses thereof | |
SG11201807252QA (en) | Anti-lag-3 antibodies | |
SG11201807025SA (en) | Crispr/cas systems for c-1 fixing bacteria | |
SG11201807444PA (en) | Transposase-mediated imaging of the accessible genome | |
SG11201909154SA (en) | Bispecific antibodies specifically binding to pd1 and lag3 | |
SG11201810003UA (en) | Using programmable dna binding proteins to enhance targeted genome modification | |
SG11201909881TA (en) | Complex comprising odh unit with integrated oxygen separation module | |
SG11201805562QA (en) | Genomic infrastructure for on-site or cloud-based dna and rna processing and analysis | |
SG11201805449PA (en) | Methods of making chimeric antigen receptor -expressing cells | |
SG11201408362SA (en) | Production of long chain polyunsaturated fatty acids in plant cells | |
SG11201805709RA (en) | Anti-pro/latent myostatin antibodies and methods of use thereof | |
SG11201906372PA (en) | Computer-implemented system and method for generating and extracting user related data stored on a blockchain | |
SG11201806284TA (en) | Low pressure separator having an internal divider and uses therefor | |
SG11201808476SA (en) | Recycling of polymer matrix composite | |
SG11201805040UA (en) | Cell reprogramming | |
SG11201408536WA (en) | METHOD FOR PRODUCING SOLUBLE FcR AS Fc-FUSION WITH INERT IMMUNOGLOBULIN Fc-REGION AND USES THEREOF | |
SG11201809530PA (en) | Method for recovery of phosphate | |
SG11201806496SA (en) | Antigen binding proteins that bind pd-l1 | |
SG11201909419XA (en) | Hydrogel for cell culture and biomedical applications | |
SG11201809374VA (en) | Cd40l-fc fusion polypeptides and methods of use thereof | |
SG11201903557SA (en) | Methods for screening b cell lymphocytes | |
SG11201907129VA (en) | Motion generating platform assembly | |
SG11201805497QA (en) | Mutated truncated von willebrand factor |