CN110174517A - The bionical immune colorimetric/SERS rapid detection method of analogue enztme - Google Patents
The bionical immune colorimetric/SERS rapid detection method of analogue enztme Download PDFInfo
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- CN110174517A CN110174517A CN201910516493.7A CN201910516493A CN110174517A CN 110174517 A CN110174517 A CN 110174517A CN 201910516493 A CN201910516493 A CN 201910516493A CN 110174517 A CN110174517 A CN 110174517A
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- bsa
- nps
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/65—Raman scattering
- G01N21/658—Raman scattering enhancement Raman, e.g. surface plasmons
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
Abstract
The present invention relates to a kind of bionical immune colorimetric/SERS rapid detection methods of analogue enztme, the technical issues of enhancing is difficult to for its weaker signal of part pesticide raman characteristic peak, and biological enzyme is solved because big by environmental disturbances, condition of storage requires high and problem of the restriction molecule trace as the scope of application of recognition component, using molecular engram array films as recognition component, the amplification of integrated simulation enzyme combined probe signal and Au NPs@MIL-101 enhance substrate, create the bionical immune colorimetric/SERS rapid detection method of analogue enztme for small molecule detection.The present invention is a kind of new detection method, and fast and convenient, easily operated, not only raw material dosage is few, uniform and convenient and efficient.
Description
Technical field
The present invention relates to detection method fields, quickly examine in particular to a kind of bionical immune colorimetric/SERS of analogue enztme
Survey method.
Background technique
In recent years, researchers propose to carry out the measurement of target with indirect SERS detection method to solve part target
The weak problem of SERS signal, there are two necessary conditions in indirect detection process: 1. SERS reports molecule；2. recognition component.Molecule
Imprinted polymer is known as " artificial antibody ", is widely used as recognition component in sensor field, such as colorimetric method, glimmering
Light, chemiluminescence, electrochemistry and surface plasma body resonant vibration etc..Enzyme linked immunosorbent assay (ELISA) (ELISA) is high in various samples
The qualitative common detection method with quantitative detecting analysis object of sensitivity.As MIPs is carried out as bionic antibody in ELISA method
The application of small molecule detection, bionical enzyme linked immunosorbent assay (ELISA) (BELISA) are come into being.
HRP label probe in BELISA method is more demanding to temperature and pH condition, however MIPs has many differences
In the property of antibody, such as pH, thermally and chemically stability, therefore, the use in conjunction between HRP and MIPs will limit MIPs as imitative
The applicability of raw antibody.It in recent years due to nano enzyme good characteristic, such as prepares simple, there is tolerance to my temperature and pH, easily
In surface modification and low cost etc., demand and application to it have been continuously increased.To sum up, we have proposed a kind of competitive simulations
The bionical immune colorimetric of enzyme/SERS rapid detection method (BNLISA).Nanometer platinum particles (PtNPs) are because it is with peroxidase activity
Property, and synthesis is simple, facilitates storage, is easy to the advantages that being surface modified by nucleic acid and protein and is widely used in simulating
In enzyme assay method research.In an experiment, the analogue (haptens) for detecting target needs to introduce to be formed with target
Competition system.Bovine serum albumin(BSA) (BSA) is because having functional group abundant can such as amino, carboxyl, mercaptan and disulphide
Being connected with other molecules by chemical bond also can be by thiol bond and electrostatic interaction and PtNPs phase separation, therefore BSA is selected to make
It is " connecting bridge " to connect analogue enztme PtNPs signal amplifying probe and target construction analog-haptens, ultimately forms analogue enztme
Signal amplifies combined probe and replaces biological enzyme probe, this combined probe, which can play competition recognition reaction but also be catalyzed TMB, to be turned
Turn to TMB2+, reach colorimetric and SERS double check.
Situ aggregation method prepares molecularly imprinted polymer array films and not only needs a large amount of template molecule and chemical reagent, right
Environment is unfriendly, and when removing removing template by ultrasonic method, blotting membrane is easy to fall off, and leads to the trace between Kong Yukong
Film is uneven.To solve the above problems, utilizing ionic liquid it is proposed that obtain uniform MIPs microballoon by precipitation polymerization
(IL) molecularly imprinted polymer array films are prepared by " grafting " method, not only raw material dosage is few, uniform and convenient and efficient.
Gold nano grain (AuNPs) has good SERS activity, and has been successfully used as SERS substrate, and MIL-101 is one
The good metal organic framework of the highly porous and thermostabilization of kind, can enhance the stability of AuNPs, improve SERS letter, therefore we are logical
It crosses local reduction way and has synthesized AuNPs@MIL-101 as SERS enhancing substrate.
Summary of the invention
The present invention is difficult to the technical issues of enhancing for its weaker signal of part pesticide raman characteristic peak, and solves biology
For enzyme because big by environmental disturbances, condition of storage requires high and problem of the restriction molecule trace as the scope of application of recognition component, with
Molecular engram array films are recognition component, and the amplification of integrated simulation enzyme combined probe signal and Au NPs@MIL-101 enhance substrate,
Create the bionical immune colorimetric/SERS rapid detection method of analogue enztme for small molecule detection.
In order to realize above-mentioned purpose of the invention, the following technical scheme is adopted:
A kind of bionical immune colorimetric/SERS rapid detection method of analogue enztme, comprising the following steps:
(1) preparation of bionical molecular engram array films
It is tested by optimization, these molecular engrams of preferred function monomer, crosslinking agent, pore-foaming agent prepare element, are connect with surface
The branch blot array film of legal system back-up；
(2) SERS enhances the preparation of substrate Au NPs@MIL-101
The synthetic method of MIL-101: nine water chromic nitrates and Isosorbide-5-Nitrae-phthalic acid are added in aqueous sodium acetate solution, will mix
Object, which is added in hydro-thermal autoclave, to be reacted, and after natural cooling, is filtered mixture using sand hopper first, is removed unreacted reactant
Matter, and MIL-101 product is cleaned, then MIL-101 is dried in a vacuum drying oven；
The synthesis process of Au NPs@MIL-101: substrate is enhanced using local reduction way preparation Au NPs@MIL-101；
(3) preparation of Pt NPs@BSA- haptens probe
The synthesis of bovine serum albumin(BSA)-haptens 1. (BSA- haptens) conjugate
BSA- hapten conjugation object between the amino of BSA and the carboxyl of hapten compound by carbodlimide method by being closed
At, the specific steps are as follows:
A. Hostathion haptens and N- hydroxysuccinimidyl hydrochlorate (NHS) are dissolved in dimethyl formamide solution (DMF), and
Stirring a period of time, solution a is obtained,
B. by N, N '-dicyclohexylcarbodiimide (DCC) is dissolved in DMF, and DCC is added dropwise in a, obtains solution
B,
C. solution b is protected from light at room temperature and is stirred overnight, through the above steps, the carboxyl of hapten compound is activated, will
Solution b centrifugation, the haptens supernatant activated；
D. BSA is dissolved into carbonate buffer solution；
E. above-mentioned activation haptens supernatant is added dropwise in BSA solution, and be stirred at room temperature, finally obtain BSA-
Haptens conjugate；
F. resisted finally, BSA- hapten conjugation object solution is dialysed 3 days with PBS buffer solution with removing unbonded half
BSA- hapten conjugation solution is mixed with the glycerol of same volume, is saved backup at -20 DEG C after purification by original；
The synthesis of 2.Pt NPs
Pt NPs is prepared with reduction of sodium citrate method, sodium citrate aqueous solution is added in platinum acid chloride solution first,
It is heated to boiling under stirring, whithin a period of time, color becomes black from light yellow, continues to stir after stopping heating, be cooled to
Room temperature；
The preparation of 3.Pt NPs@BSA- haptens probe
The BSA- haptens solution of different volumes, is then added to by the pH value for adjusting Pt NPs solution with potassium carbonate first
In Pt NPs solution, after standing 12h, centrifugation, and sediment PBS buffer solution is dissolved, to remove unbonded BSA- half
Then the Pt@BSA- haptens probe of concentration it is molten to be resuspended in the PBS buffering that 200 μ L contain 1%PEG 20000 by antigen
In liquid, saved at 4 DEG C stand-by；
(5) detection process
Bionical 96 hole array plate of molecular engram is washed three times with the PBS buffer solution containing polysorbas20 first, removes impurity,
Specific reaction detection process is as follows:
1. diluting Hostathion standard solution and probe solution with work buffer solution, and it is imitative respectively to take proper volume to be added to
In the micropore of raw array board, a period of time is reacted at room temperature；
2. wash bionic array plate with PBST removes unbonded pesticide molecule and simulation enzyme probe three times；
3. then, ELISA substrate TMB is added in micropore and is reacted at room temperature, then it is added into each hole
H2SO4 terminate liquid terminates reaction；
4. finally, being recorded in the absorbance value at 450nm by 96 plate reader of Lab Systems；
5. SERS measures TMB2+ solution, it is added without 4 terminate liquid of H2SO, firstly, by TMB2+ solution and Au NPs@
MIL-101 enhances substrate mixing, and incubation reaction, then measures SERS " fingerprint " information by Portable Raman spectrometer.
The present invention is a kind of new detection method, fast and convenient, easily operated, and not only raw material dosage is few, uniform and conveniently
Fast.
Detailed description of the invention
Fig. 1 is the detectability analysis of competitive BNLISA method.
Specific embodiment
In order to make those skilled in the art that the present invention may be better understood, with reference to the accompanying drawings and examples to this hair
Bright technical solution further illustrates.
Using Hostathion pesticide as representative, competitive the potential of BNLISA method is tested by colorimetric method and SERS method and is answered
With as shown in Figure 1, a. is with colorimetric method for determining concentration range in 0~10000ngmL-1Hostathion ultraviolet-ray visible absorbing light
Spectrum；B. the calibration curve that the absorbance value at 450nm and log concentration mapping obtain；C. existed with SERS method measurement concentration range
0~10000ngmL-1Hostathion SERS spectra；D. in 558cm-1The SERS signal intensity at place is obtained with log concentration mapping
The calibration curve obtained.The Hostathion standard solution for configuring various concentration records TMB by BNLISA competitive reaction2+?
Visible absorbance value (OD) and 558cm under 570nm-1Locate the SERS signal intensity at peak, and draw standard curve respectively,
The result shows that colorimetric method is in 5-1000ngmL-1In concentration range, there are good between inhibiting rate and Hostathion log concentration
Linear relationship；SERS method is in 1-10000ngmL-1In concentration range, 558cm-1Locate the SERS signal intensity and Hostathion at peak
There is good linear relationship, the detection limit of two methods is all 1ngmL between the logarithm of concentration-1。
In order to assess accuracy and reliability of the BNLISA method in actual sample detection, having studied spiked levels is
10,100 and 500ngmL-1When, the detection (n=3) of tap water water sample；And in pears sample substrate, addition concentration is 20,
100 and 500ngmL-1When recovery testu.The results are shown in Table 1, the BNLISA rate of recovery of colorimetric method 75.6~
Between 109.8%, for the rate of recovery of SERS method between 65.6~110.7%, RSD is respectively 7.8%~11.2% He
10.1%~15.2%.These are the result shows that BNLISA method can be used for the detection of Hostathion in water and pears sample.
1 BNLISA of table is applied to the detection of Hostathion in water sample and pears sample
The preferred embodiment of the present invention above described embodiment only expresses, the description thereof is more specific and detailed, but simultaneously
Limitations on the scope of the patent of the present invention therefore cannot be interpreted as.It should be pointed out that for those of ordinary skill in the art
For, without departing from the inventive concept of the premise, several deformations can also be made, improves and substitutes, these belong to this hair
Bright protection scope.Therefore, the scope of protection of the patent of the invention shall be subject to the appended claims.
Claims (1)
1. a kind of bionical immune colorimetric/SERS rapid detection method of analogue enztme, which comprises the following steps:
(1) preparation of bionical molecular engram array films
It is tested by optimization, these molecular engrams of preferred function monomer, crosslinking agent, pore-foaming agent prepare element, with surface grafting method
Prepare molecular engram array films；
(2) SERS enhances the preparation of substrate Au NPs@MIL-101
The synthetic method of MIL-101: being added nine water chromic nitrates and Isosorbide-5-Nitrae-phthalic acid in aqueous sodium acetate solution, by mixture plus
Enter and reacted in hydro-thermal autoclave, after natural cooling, filter mixture using sand hopper first, removes unreacting substance, and
And cleaning MIL-101 product, then MIL-101 is dried in a vacuum drying oven；
The synthesis process of Au NPs@MIL-101: substrate is enhanced using local reduction way preparation Au NPs@MIL-101；
(3) preparation of Pt NPs@BSA- haptens probe
1) synthesis of bovine serum albumin(BSA)-haptens (BSA- haptens) conjugate
BSA- hapten conjugation object is had by being synthesized between the amino of BSA and the carboxyl of hapten compound by carbodlimide method
Steps are as follows for body:
A. Hostathion haptens and N- hydroxysuccinimidyl hydrochlorate (NHS) are dissolved in dimethyl formamide solution (DMF), and stirred
For a period of time, solution a is obtained,
B. by N, N '-dicyclohexylcarbodiimide (DCC) is dissolved in DMF, and DCC is added dropwise in a, obtains solution b,
C. solution b is protected from light at room temperature and is stirred overnight, through the above steps, the carboxyl of hapten compound is activated, by solution b
Centrifugation, the haptens supernatant activated；
D. BSA is dissolved into carbonate buffer solution；
E. above-mentioned activation haptens supernatant is added dropwise in BSA solution, and be stirred at room temperature, the final BSA- half that obtains resists
Former conjugate；
F. pure finally, by BSA- hapten conjugation object solution with the dialysis of PBS buffer solution 3 days to remove unbonded haptens
After change, BSA- hapten conjugation solution is mixed with the glycerol of same volume, is saved backup at -20 DEG C；
2) synthesis of Pt NPs
Pt NPs is prepared with reduction of sodium citrate method, sodium citrate aqueous solution is added in platinum acid chloride solution first, is being stirred
Under be heated to boiling, whithin a period of time, color becomes black from light yellow, continues to stir after stopping heating, be cooled to room temperature；
3) preparation of Pt NPs@BSA- haptens probe
The pH value for adjusting Pt NPs solution with potassium carbonate first, is then added to Pt for the BSA- haptens solution of different volumes
In NPs solution, after standing 12h, centrifugation, and sediment PBS buffer solution is dissolved, it is anti-to remove unbonded BSA- half
Then the Pt@BSA- haptens probe of concentration is resuspended in the PBS buffer solution that 200 μ L contain 1%PEG 20000 by original
In, it is saved at 4 DEG C stand-by；
(4) detection process
Bionical 96 hole array plate of molecular engram is washed three times with the PBS buffer solution containing polysorbas20 first, removes impurity, specifically
Reaction detection process is as follows:
1. diluting Hostathion standard solution and probe solution with work buffer solution, and proper volume is respectively taken to be added to bionical battle array
In the micropore of strake, a period of time is reacted at room temperature；
2. wash bionic array plate with PBST removes unbonded pesticide molecule and simulation enzyme probe three times；
3. then, ELISA substrate TMB is added in micropore and is reacted at room temperature, H then is added into each hole2SO4Eventually
Only liquid terminates reaction；
4. finally, being recorded in the absorbance value at 450nm by Lab Systems96 plate reader；
5. SERS measures TMB2+When solution, it is added without H2SO4Terminate liquid, firstly, by TMB2+Solution and Au NPs@MIL-101 increase
The mixing of strong basis bottom, and incubation reaction, then measure SERS " fingerprint " information by Portable Raman spectrometer.
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111443076A (en) * | 2020-06-01 | 2020-07-24 | 中国农业科学院农业质量标准与检测技术研究所 | Tyrosinase inhibition-based glyphosate detection system and SERS detection method |
| CN113984861A (en) * | 2021-10-25 | 2022-01-28 | 山东农业大学 | Electrochemical sensor for in-situ analysis and detection of heavy metal copper ions in soil solution |
| RU2797004C1 (en) * | 2022-12-19 | 2023-05-30 | федеральное государственное автономное образовательное учреждение высшего образования "Московский физико-технический институт (национальный исследовательский университет)" | Method for fabricating substrates for giant raman spectroscopy |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186645A (en) * | 2007-11-23 | 2008-05-28 | 东华大学 | Fluoranthene artificial immunogen FL-BSA and its preparation and use |
| CN108387728A (en) * | 2018-01-19 | 2018-08-10 | 中国农业科学院农业质量标准与检测技术研究所 | A kind of bionical enzyme-linked immuno sorbent assay kit of Hostathion and detection method |
| CN108732158A (en) * | 2018-06-13 | 2018-11-02 | 中国农业科学院农业质量标准与检测技术研究所 | The remaining MIPs-SERS detection methods of triazine suitable for detection agricultural product |
| CN109187490A (en) * | 2018-11-09 | 2019-01-11 | 中国农业科学院农业质量标准与检测技术研究所 | Based on SERS technology detection Atrazine, chlopyrifos, the method for triazolone and kit |
| US20190056328A1 (en) * | 2016-02-22 | 2019-02-21 | Agency For Science, Technology And Research | Method for detecting an analyte using surface enhanced raman spectroscopy |
-
2019
- 2019-06-14 CN CN201910516493.7A patent/CN110174517A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101186645A (en) * | 2007-11-23 | 2008-05-28 | 东华大学 | Fluoranthene artificial immunogen FL-BSA and its preparation and use |
| US20190056328A1 (en) * | 2016-02-22 | 2019-02-21 | Agency For Science, Technology And Research | Method for detecting an analyte using surface enhanced raman spectroscopy |
| CN108387728A (en) * | 2018-01-19 | 2018-08-10 | 中国农业科学院农业质量标准与检测技术研究所 | A kind of bionical enzyme-linked immuno sorbent assay kit of Hostathion and detection method |
| CN108732158A (en) * | 2018-06-13 | 2018-11-02 | 中国农业科学院农业质量标准与检测技术研究所 | The remaining MIPs-SERS detection methods of triazine suitable for detection agricultural product |
| CN109187490A (en) * | 2018-11-09 | 2019-01-11 | 中国农业科学院农业质量标准与检测技术研究所 | Based on SERS technology detection Atrazine, chlopyrifos, the method for triazolone and kit |
Non-Patent Citations (5)
| Title |
|---|
| YANZHENG CAI等: "Core−Shell Au@Metal−Organic Frameworks for Promoting Raman Detection Sensitivity of Methenamine", 《ACS APPLIED MATERIALS & INTERFACES》 * |
| YIHUI HU等: "Surface-Enhanced Raman Scattering Active Gold Nanoparticles with Enzyme-Mimicking Activities for Measuring Glucose and Lactate in Living Tissues", 《ACS NANO》 * |
| YULING HU等: "Fabrication of Gold Nanoparticle-Embedded Metal−Organic Framework for Highly Sensitive Surface-Enhanced Raman Scattering Detection", 《ANALYTICAL CHEMISTRY》 * |
| 余家会等: "《纳米生物医药》", 31 December 2011, 华东理工大学出版社 * |
| 周凌云等: "《功能化介孔材料捕集CO2研究》", 31 March 2019, 中国科学技术出版社 * |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111443076A (en) * | 2020-06-01 | 2020-07-24 | 中国农业科学院农业质量标准与检测技术研究所 | Tyrosinase inhibition-based glyphosate detection system and SERS detection method |
| CN111443076B (en) * | 2020-06-01 | 2023-01-20 | 中国农业科学院农业质量标准与检测技术研究所 | Glyphosate detection system based on tyrosinase inhibition and SERS detection method |
| CN113984861A (en) * | 2021-10-25 | 2022-01-28 | 山东农业大学 | Electrochemical sensor for in-situ analysis and detection of heavy metal copper ions in soil solution |
| CN113984861B (en) * | 2021-10-25 | 2022-11-22 | 山东农业大学 | Application of electrochemical sensor in-situ analysis and detection of heavy metal copper ions in soil solution |
| RU2797004C1 (en) * | 2022-12-19 | 2023-05-30 | федеральное государственное автономное образовательное учреждение высшего образования "Московский физико-технический институт (национальный исследовательский университет)" | Method for fabricating substrates for giant raman spectroscopy |
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Application publication date: 20190827 |