SG11201804699WA - Open-top microfluidic device with structural anchors - Google Patents

Open-top microfluidic device with structural anchors

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Publication number
SG11201804699WA
SG11201804699WA SG11201804699WA SG11201804699WA SG11201804699WA SG 11201804699W A SG11201804699W A SG 11201804699WA SG 11201804699W A SG11201804699W A SG 11201804699WA SG 11201804699W A SG11201804699W A SG 11201804699WA SG 11201804699W A SG11201804699W A SG 11201804699WA
Authority
SG
Singapore
Prior art keywords
international
street
carroll
pct
open
Prior art date
Application number
SG11201804699WA
Inventor
Daniel Levner
Chris Hinojosa
Norman Wen
Antonio Varone
Justin Nguyen
Lina Williamson
S Kerns
Katia Karalis
Geraldine Hamilton
Carol Lucchesi
Original Assignee
Emulate Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Emulate Inc filed Critical Emulate Inc
Publication of SG11201804699WA publication Critical patent/SG11201804699WA/en

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    • C12M3/00—Tissue, human, animal or plant cell, or virus culture apparatus
    • C12M3/04—Tissue, human, animal or plant cell, or virus culture apparatus with means providing thin layers
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    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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Abstract

INTERNATIONAL APPLICATION PUBLISHED UNDER THE PATENT COOPERATION TREATY (PCT) (19) World Intellectual Property Organization International Bureau IIIIII Hit II EH DID IIIII DEEM II 11111111111111111111111111111111R1111111 (10) International Publication Number WO 2017/096297 Al WIPO I PCT (43) International Publication Date 8 June 2017 (08.06.2017) (74) Agents: CARROLL, Peter G. et al.; Medlen & Carroll, LLP, 1440 Broadway, Suite 510, Oakland, CA 94612 (US). (81) Designated States (unless otherwise indicated, for every kind of national protection available): AE, AG, AL, AM, AO, AT, AU, AZ, BA, BB, BG, BH, BN, BR, BW, BY, BZ, CA, CH, CL, CN, CO, CR, CU, CZ, DE, DJ, DK, DM, DO, DZ, EC, EE, EG, ES, FI, GB, GD, GE, GH, GM, GT, HN, HR, HU, ID, IL, IN, IR, IS, JP, KE, KG, KN, KP, KR, KW, KZ, LA, LC, LK, LR, LS, LU, LY, MA, MD, ME, MG, MK, MN, MW, MX, MY, MZ, NA, NG, NI, NO, NZ, OM, PA, PE, PG, PH, PL, PT, QA, RO, RS, RU, RW, SA, SC, SD, SE, SG, SK, SL, SM, ST, SV, SY, TH, TJ, TM, TN, TR, TT, TZ, UA, UG, US, UZ, VC, VN, ZA, ZM, ZW. (84) Designated States (unless otherwise indicated, for every kind of regional protection available): ARIPO (BW, GH, GM, KE, LR, LS, MW, MZ, NA, RW, SD, SL, ST, SZ, TZ, UG, ZM, ZW), Eurasian (AM, AZ, BY, KG, KZ, RU, TJ, TM), European (AL, AT, BE, BG, CH, CY, CZ, DE, DK, EE, ES, FI, FR, GB, GR, HR, HU, IE, IS, IT, LT, LU, LV, MC, MK, MT, NL, NO, PL, PT, RO, RS, SE, SI, SK, SM, TR), OAPI (BF, BJ, CF, CG, CI, CM, GA, GN, GQ, GW, KM, ML, MR, NE, SN, TD, TG). Published: with international search report (Art 21(3)) (51) International Patent Classification: C12M 3/00 (2006.01) C12M 3/06 (2006.01) (21) International Application GO1N 33/50 (2006.01) Number: PCT/US2016/064814 (22) International Filing Date: 2 December 2016 (02.12.2016) English English (25) Filing Language: (26) Publication Language: (30) Priority Data: 62/263,492 4 December 2015 (04.12.2015) US (71) Applicant: EMULATE, INC. [US/US]; 27 Drydock, Bo- ston, MA 02110 (US). (72) Inventors: LEVNER, Daniel; 55 Sewall Ave, 5C, Brook- line, MA 02446 (US) HINOJOSA, Chris; 69 Holworthy Street, Cambirdge, MA 02138 (US). WEN, Norman; 4975 Washington Street, Unit 118, West Roxbury, MA 02132 (US). VARONE, Antonio; 865 Boylston Street, Newton, MA 02461 (US). NGUYEN, Justin; 45 Cross St., Apt 24, Medford, MA 02155 (US). WILLIAMSON, Lina; 273 Brown Bear, Chapel Hill, NC 27517 (US). KERNS, Jordan; 7 Heritage Dr, Reading, MA 01867 (US). KARALIS, Katia; 9 Colbourn Crescent #2, Brookline, MA 02445 (US). HAMILTON, Geraldine; 222 Hurley Street, Cambridge, MA 02141 (US). LUCCHESI, Carol; 16 Carroll Ct, Westwood, MA 02090 (US). 131 (54) Title: OPEN-TOP MICROFLUIDIC DEVICE WITH STRUCTURAL ANCHORS 100 117 102 137 134 101 103 127 140 128 W O 20 17 /096 297 Al 102 136 126 118 133 FIG.1 112 (57) : A microfluidic device is contemplated comprising an open-top cavity with structural anchors on the vertical wall sur - faces that serve to prevent gel shrinkage-induced delamination, a porous membrane (optionally stretchable) positioned in the middle over a microfluidic channel(s). The device is particularly suited to the growth of cells mimicking dermal layers.
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