SG11201609053YA - Multiplexed single cell gene expression analysis using template switch and tagmentation - Google Patents
Multiplexed single cell gene expression analysis using template switch and tagmentationInfo
- Publication number
- SG11201609053YA SG11201609053YA SG11201609053YA SG11201609053YA SG11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA SG 11201609053Y A SG11201609053Y A SG 11201609053YA
- Authority
- SG
- Singapore
- Prior art keywords
- tagmentation
- gene expression
- single cell
- expression analysis
- cell gene
- Prior art date
Links
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1034—Isolating an individual clone by screening libraries
- C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
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- C—CHEMISTRY; METALLURGY
- C40—COMBINATORIAL TECHNOLOGY
- C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
- C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
- C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201461985983P | 2014-04-29 | 2014-04-29 | |
| US201461987433P | 2014-05-01 | 2014-05-01 | |
| PCT/US2015/028062 WO2015168161A2 (fr) | 2014-04-29 | 2015-04-28 | Analyse de l'expression de gènes de cellules isolées multiplexées par commutation de matrice et fragmentation et étiquetage (tagmentation) |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201609053YA true SG11201609053YA (en) | 2016-11-29 |
Family
ID=53055134
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201912516SA SG10201912516SA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
| SG11201609053YA SG11201609053YA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
Family Applications Before (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG10201912516SA SG10201912516SA (en) | 2014-04-29 | 2015-04-28 | Multiplexed single cell gene expression analysis using template switch and tagmentation |
Country Status (8)
| Country | Link |
|---|---|
| US (2) | US20170044525A1 (fr) |
| EP (2) | EP3680333A1 (fr) |
| JP (1) | JP6412954B2 (fr) |
| CN (1) | CN106459967A (fr) |
| AU (4) | AU2015253299B2 (fr) |
| CA (2) | CA2947426C (fr) |
| SG (2) | SG10201912516SA (fr) |
| WO (1) | WO2015168161A2 (fr) |
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-
2015
- 2015-04-28 US US15/307,329 patent/US20170044525A1/en not_active Abandoned
- 2015-04-28 SG SG10201912516SA patent/SG10201912516SA/en unknown
- 2015-04-28 WO PCT/US2015/028062 patent/WO2015168161A2/fr active Application Filing
- 2015-04-28 CA CA2947426A patent/CA2947426C/fr active Active
- 2015-04-28 SG SG11201609053YA patent/SG11201609053YA/en unknown
- 2015-04-28 CA CA3060708A patent/CA3060708A1/fr active Pending
- 2015-04-28 CN CN201580033171.7A patent/CN106459967A/zh active Pending
- 2015-04-28 JP JP2016565421A patent/JP6412954B2/ja active Active
- 2015-04-28 EP EP20154378.2A patent/EP3680333A1/fr active Pending
- 2015-04-28 AU AU2015253299A patent/AU2015253299B2/en active Active
- 2015-04-28 EP EP15721102.0A patent/EP3137601B1/fr active Active
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2018
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2020
- 2020-10-23 US US17/078,900 patent/US20210079386A1/en active Pending
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|---|---|
| CN106459967A (zh) | 2017-02-22 |
| AU2021203246A1 (en) | 2021-06-10 |
| EP3137601A2 (fr) | 2017-03-08 |
| CA2947426C (fr) | 2020-01-07 |
| WO2015168161A3 (fr) | 2015-12-30 |
| AU2024201323A1 (en) | 2024-03-21 |
| EP3680333A1 (fr) | 2020-07-15 |
| JP2017514487A (ja) | 2017-06-08 |
| CA2947426A1 (fr) | 2015-11-05 |
| WO2015168161A2 (fr) | 2015-11-05 |
| US20170044525A1 (en) | 2017-02-16 |
| AU2018226493B2 (en) | 2021-02-25 |
| JP6412954B2 (ja) | 2018-10-24 |
| AU2021203246B2 (en) | 2023-11-30 |
| US20210079386A1 (en) | 2021-03-18 |
| AU2015253299B2 (en) | 2018-06-14 |
| AU2015253299A1 (en) | 2016-11-17 |
| SG10201912516SA (en) | 2020-02-27 |
| EP3137601B1 (fr) | 2020-04-08 |
| AU2018226493A1 (en) | 2018-09-27 |
| CA3060708A1 (fr) | 2015-11-05 |
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