SG10201701747YA - Method for isolating rna including small rna with high yield - Google Patents

Method for isolating rna including small rna with high yield

Info

Publication number
SG10201701747YA
SG10201701747YA SG10201701747YA SG10201701747YA SG10201701747YA SG 10201701747Y A SG10201701747Y A SG 10201701747YA SG 10201701747Y A SG10201701747Y A SG 10201701747YA SG 10201701747Y A SG10201701747Y A SG 10201701747YA SG 10201701747Y A SG10201701747Y A SG 10201701747YA
Authority
SG
Singapore
Prior art keywords
rna
high yield
including small
isolating
small rna
Prior art date
Application number
SG10201701747YA
Other languages
English (en)
Inventor
Martin Schlumpberger
Stefanie Schröer
Vera Holländer
Original Assignee
Qiagen Gmbh
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Qiagen Gmbh filed Critical Qiagen Gmbh
Publication of SG10201701747YA publication Critical patent/SG10201701747YA/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/1013—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by using magnetic beads

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
SG10201701747YA 2012-09-03 2013-09-03 Method for isolating rna including small rna with high yield SG10201701747YA (en)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US201261696244P 2012-09-03 2012-09-03
EP12182741 2012-09-03

Publications (1)

Publication Number Publication Date
SG10201701747YA true SG10201701747YA (en) 2017-04-27

Family

ID=46796430

Family Applications (2)

Application Number Title Priority Date Filing Date
SG10201701747YA SG10201701747YA (en) 2012-09-03 2013-09-03 Method for isolating rna including small rna with high yield
SG11201500655VA SG11201500655VA (en) 2012-09-03 2013-09-03 Method for isolating rna including small rna with high yield

Family Applications After (1)

Application Number Title Priority Date Filing Date
SG11201500655VA SG11201500655VA (en) 2012-09-03 2013-09-03 Method for isolating rna including small rna with high yield

Country Status (8)

Country Link
US (1) US10329553B2 (zh)
EP (1) EP2893015B1 (zh)
JP (1) JP6440616B2 (zh)
CN (1) CN104603267B (zh)
AU (1) AU2013310861B2 (zh)
CA (1) CA2880136C (zh)
SG (2) SG10201701747YA (zh)
WO (1) WO2014033326A1 (zh)

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US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
AU2015289027B2 (en) * 2014-07-17 2021-08-19 Qiagen Gmbh Method for isolating RNA with high yield
JP2017537616A (ja) * 2014-11-14 2017-12-21 コーニング インコーポレイテッド インビトロ転写後のｒｎａ精製のための方法及びキット
EP3368669B1 (en) * 2015-10-28 2021-03-10 Mirnagreen S.r.l. Methods for extracting bioactive small rnas from plants and mushrooms
CN105385681B (zh) * 2015-12-29 2018-07-17 中国海洋大学 一种用于鲆鲽鱼类血液rna提取的裂解液
US20180135040A1 (en) 2016-02-16 2018-05-17 Life Magnetics, Inc. Methods for separating nucleic acids with graphene coated magnetic beads
CN110621791A (zh) * 2017-02-27 2019-12-27 医学诊断公司 用于纯化和扩增核酸的系统和方法
JP7285257B2 (ja) * 2018-07-06 2023-06-01 社会医療法人大雄会 小型ｒｎａの抽出効率を改善するための組成物および方法
WO2021067422A1 (en) * 2019-10-01 2021-04-08 Showa Denko Materials (America), Inc. Filter-based extracellular vesicle nucleic acid isolation method
CN112662663B (zh) * 2021-01-27 2022-11-08 苏州赛普生物科技有限公司 一种适于常温运输的rna提取试剂盒及提取方法
WO2023198909A1 (en) * 2022-04-15 2023-10-19 Quantoom Biosciences S.A. Methods for separation and/or purification of nucleic acids

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ES2066851T3 (es) 1988-05-24 1995-03-16 Anagen Uk Ltd Particulas atraibles magneticamente y metodo de preparacion.
DK0743950T3 (da) 1994-02-11 2002-03-25 Qiagen Gmbh Fremgangsmåde til adskillelse af dobbeltstrengede/enkeltstrengede nukleinsyre-strukturer
DE19520398B4 (de) 1995-06-08 2009-04-16 Roche Diagnostics Gmbh Magnetisches Pigment
EP1260595B1 (en) 1995-07-07 2006-09-13 Toyo Boseki Kabushiki Kaisha Nucleic acid-bondable magnetic carrier and method for isolating nucleic acid using the same
KR100483498B1 (ko) 1996-02-14 2005-07-18 악조 노벨 엔.브이. 핵산물질의분리및증폭방법
ES2263185T3 (es) * 1996-12-10 2006-12-01 Purdue Research Foundation Biomaterial derivado de tejido hepatico de vertebrados.
US6027945A (en) 1997-01-21 2000-02-22 Promega Corporation Methods of isolating biological target materials using silica magnetic particles
DK0961653T3 (da) 1997-01-21 2003-03-31 Grace W R & Co Silicaadsorbent på magnetisk substrat
ZA99493B (en) * 1998-01-30 1999-07-22 Akzo Nobel Nv Method for the isolation of nucleic acid.
AU6380001A (en) 2000-03-24 2001-10-03 Qiagen Gmbh Porous ferro- or ferrimagnetic glass particles for isolating molecules
DE10031236A1 (de) * 2000-06-27 2002-01-10 Qiagen Gmbh Verwendung von Carbonsäuren und anderen Additiven in Kombination mit kationischen Verbindungen zur Stabilisierung von Nukleinsäuren in biologischen Materialien
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GB0215185D0 (en) 2002-07-01 2002-08-07 Genovision As Binding a target substance
US20050059024A1 (en) 2003-07-25 2005-03-17 Ambion, Inc. Methods and compositions for isolating small RNA molecules
ATE554166T1 (de) * 2003-07-25 2012-05-15 Life Technologies Corp Verfahren und zusammensetzungen zur herstellung von rna aus einer fixierten probe
US20050026153A1 (en) * 2003-07-31 2005-02-03 Iannotti Claudia A. Devices and methods for isolating RNA
US20050054847A1 (en) * 2003-08-01 2005-03-10 Invitrogen Corporation Compositions and methods for preparing short RNA molecules and other nucleic acids
MXPA06001646A (es) * 2003-08-12 2006-05-12 Massachusetts Inst Technology Metodos y dispositivos para la preparacion de muestras.
CN1284852C (zh) * 2004-09-09 2006-11-15 武汉大学 一种莲藕组织总rna的快速提取方法
DE102004045332B3 (de) 2004-09-16 2006-03-09 Macherey, Nagel Gmbh & Co. Handelsgesellschaft Verfahren zur Isolierung und Trennung von einzel- und doppelsträngigen Nucleinsäuren aus einer diese Nucleinsäuren enthaltenden Probe sowie Trennsäule zur Durchführung dieses Verfahrens
DE102007035250A1 (de) 2007-07-27 2009-01-29 Qiagen Gmbh Verfahren zum Abtrennen von nicht-proteinhaltigen Biomolekülen, insbesondere Nukleinsäuren aus proteinhaltigen Proben
US7871764B1 (en) * 2008-03-18 2011-01-18 The United States Of America As Represented By The United States Department Of Energy Universal nucleic acids sample preparation method for cells, spores and their mixture
CN101561415B (zh) * 2008-04-18 2012-07-18 陈定虎 一种同时检测香蕉束顶病毒和花叶心腐病毒的检测方法
CN101492485B (zh) * 2009-03-13 2012-02-08 北京林业大学 一种提取裸子植物组织中rna的方法
WO2011104032A1 (en) 2010-02-26 2011-09-01 Qiagen Gmbh Method for isolating rna from a rna and dna containing sample
EP2407540A1 (en) * 2010-07-15 2012-01-18 Qiagen GmbH Method for purifying a target nucleic acid
CN103097532B (zh) * 2010-09-02 2018-04-27 恰根有限公司 高产量分离包括小靶核酸在内的靶核酸的方法
CN102344923A (zh) * 2011-10-26 2012-02-08 浙江大学舟山海洋研究中心 海地瓜消化道总rna快速提取方法

Also Published As

Publication number Publication date
CA2880136A1 (en) 2014-03-06
SG11201500655VA (en) 2015-05-28
US20150232831A1 (en) 2015-08-20
EP2893015A1 (en) 2015-07-15
US10329553B2 (en) 2019-06-25
WO2014033326A1 (en) 2014-03-06
CA2880136C (en) 2023-08-15
EP2893015B1 (en) 2018-05-02
CN104603267B (zh) 2019-08-27
AU2013310861B2 (en) 2019-02-14
CN104603267A (zh) 2015-05-06
JP2015526100A (ja) 2015-09-10
AU2013310861A1 (en) 2015-02-12
JP6440616B2 (ja) 2018-12-19

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