SE501439C2 - Sätt och anordning för analys av polynukleotidsekvenser - Google Patents
Sätt och anordning för analys av polynukleotidsekvenserInfo
- Publication number
- SE501439C2 SE501439C2 SE9302152A SE9302152A SE501439C2 SE 501439 C2 SE501439 C2 SE 501439C2 SE 9302152 A SE9302152 A SE 9302152A SE 9302152 A SE9302152 A SE 9302152A SE 501439 C2 SE501439 C2 SE 501439C2
- Authority
- SE
- Sweden
- Prior art keywords
- polynucleotide
- sequence
- sequences
- base
- terminating
- Prior art date
Links
- 108091033319 polynucleotide Proteins 0.000 title claims abstract description 33
- 102000040430 polynucleotide Human genes 0.000 title claims abstract description 33
- 239000002157 polynucleotide Substances 0.000 title claims abstract description 33
- 238000000034 method Methods 0.000 title claims abstract description 19
- 239000002773 nucleotide Substances 0.000 claims abstract description 20
- 125000003729 nucleotide group Chemical group 0.000 claims abstract description 20
- 239000012634 fragment Substances 0.000 claims abstract description 11
- 238000006243 chemical reaction Methods 0.000 claims abstract description 6
- 238000009396 hybridization Methods 0.000 claims abstract description 3
- 108091034117 Oligonucleotide Proteins 0.000 claims description 20
- 239000007790 solid phase Substances 0.000 claims description 19
- 238000004458 analytical method Methods 0.000 claims description 10
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 claims description 8
- 108010090804 Streptavidin Proteins 0.000 claims description 5
- 229960002685 biotin Drugs 0.000 claims description 4
- 235000020958 biotin Nutrition 0.000 claims description 4
- 239000011616 biotin Substances 0.000 claims description 4
- 239000000126 substance Substances 0.000 claims description 3
- 239000005546 dideoxynucleotide Substances 0.000 claims description 2
- 239000011541 reaction mixture Substances 0.000 claims description 2
- 230000009870 specific binding Effects 0.000 claims description 2
- 108090001008 Avidin Proteins 0.000 claims 1
- 239000007787 solid Substances 0.000 abstract description 2
- 239000003155 DNA primer Substances 0.000 abstract 3
- 238000000137 annealing Methods 0.000 abstract 1
- 239000013615 primer Substances 0.000 abstract 1
- 230000035772 mutation Effects 0.000 description 18
- 108020004414 DNA Proteins 0.000 description 15
- JLCPHMBAVCMARE-UHFFFAOYSA-N [3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[3-[[3-[[3-[[3-[[3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-[[5-(2-amino-6-oxo-1H-purin-9-yl)-3-hydroxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxyoxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(5-methyl-2,4-dioxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(6-aminopurin-9-yl)oxolan-2-yl]methoxy-hydroxyphosphoryl]oxy-5-(4-amino-2-oxopyrimidin-1-yl)oxolan-2-yl]methyl [5-(6-aminopurin-9-yl)-2-(hydroxymethyl)oxolan-3-yl] hydrogen phosphate Polymers Cc1cn(C2CC(OP(O)(=O)OCC3OC(CC3OP(O)(=O)OCC3OC(CC3O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c3nc(N)[nH]c4=O)C(COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3COP(O)(=O)OC3CC(OC3CO)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3ccc(N)nc3=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cc(C)c(=O)[nH]c3=O)n3cc(C)c(=O)[nH]c3=O)n3ccc(N)nc3=O)n3cc(C)c(=O)[nH]c3=O)n3cnc4c3nc(N)[nH]c4=O)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)n3cnc4c(N)ncnc34)O2)c(=O)[nH]c1=O JLCPHMBAVCMARE-UHFFFAOYSA-N 0.000 description 9
- 238000007792 addition Methods 0.000 description 8
- 239000003550 marker Substances 0.000 description 6
- 238000003776 cleavage reaction Methods 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 230000007017 scission Effects 0.000 description 4
- 108091032973 (ribonucleotides)n+m Proteins 0.000 description 3
- 108091028043 Nucleic acid sequence Proteins 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 239000011521 glass Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 108010091358 Hypoxanthine Phosphoribosyltransferase Proteins 0.000 description 2
- 238000012300 Sequence Analysis Methods 0.000 description 2
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 2
- NINIDFKCEFEMDL-UHFFFAOYSA-N Sulfur Chemical compound [S] NINIDFKCEFEMDL-UHFFFAOYSA-N 0.000 description 2
- 125000002777 acetyl group Chemical group [H]C([H])([H])C(*)=O 0.000 description 2
- 230000027455 binding Effects 0.000 description 2
- 230000008878 coupling Effects 0.000 description 2
- 238000010168 coupling process Methods 0.000 description 2
- 238000005859 coupling reaction Methods 0.000 description 2
- 230000037430 deletion Effects 0.000 description 2
- 238000012217 deletion Methods 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 229910052717 sulfur Inorganic materials 0.000 description 2
- 239000011593 sulfur Substances 0.000 description 2
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 1
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 1
- 201000003883 Cystic fibrosis Diseases 0.000 description 1
- 102000053602 DNA Human genes 0.000 description 1
- 238000001712 DNA sequencing Methods 0.000 description 1
- 108010014303 DNA-directed DNA polymerase Proteins 0.000 description 1
- 102000016928 DNA-directed DNA polymerase Human genes 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 108090000371 Esterases Proteins 0.000 description 1
- 108700024394 Exon Proteins 0.000 description 1
- 208000015872 Gaucher disease Diseases 0.000 description 1
- 208000026350 Inborn Genetic disease Diseases 0.000 description 1
- 102100034343 Integrase Human genes 0.000 description 1
- 208000024556 Mendelian disease Diseases 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 108020002230 Pancreatic Ribonuclease Proteins 0.000 description 1
- 102000005891 Pancreatic ribonuclease Human genes 0.000 description 1
- 108010076504 Protein Sorting Signals Proteins 0.000 description 1
- 108010092799 RNA-directed DNA polymerase Proteins 0.000 description 1
- 208000022292 Tay-Sachs disease Diseases 0.000 description 1
- 230000005856 abnormality Effects 0.000 description 1
- 230000004075 alteration Effects 0.000 description 1
- 150000001412 amines Chemical class 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 208000005980 beta thalassemia Diseases 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 230000000295 complement effect Effects 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 239000000975 dye Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 150000002118 epoxides Chemical class 0.000 description 1
- 230000037433 frameshift Effects 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 125000001183 hydrocarbyl group Chemical group 0.000 description 1
- 238000003780 insertion Methods 0.000 description 1
- 230000037431 insertion Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 102000039446 nucleic acids Human genes 0.000 description 1
- 108020004707 nucleic acids Proteins 0.000 description 1
- 150000007523 nucleic acids Chemical class 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 108700025694 p53 Genes Proteins 0.000 description 1
- KHIWWQKSHDUIBK-UHFFFAOYSA-N periodic acid Chemical compound OI(=O)(=O)=O KHIWWQKSHDUIBK-UHFFFAOYSA-N 0.000 description 1
- 235000021317 phosphate Nutrition 0.000 description 1
- 125000002467 phosphate group Chemical group [H]OP(=O)(O[H])O[*] 0.000 description 1
- 150000003013 phosphoric acid derivatives Chemical class 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 125000002652 ribonucleotide group Chemical group 0.000 description 1
- 125000000548 ribosyl group Chemical group C1([C@H](O)[C@H](O)[C@H](O1)CO)* 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000000377 silicon dioxide Substances 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
- C12Q1/6874—Methods for sequencing involving nucleic acid arrays, e.g. sequencing by hybridisation
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6858—Allele-specific amplification
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Health & Medical Sciences (AREA)
- Biophysics (AREA)
- General Engineering & Computer Science (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Physics & Mathematics (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biotechnology (AREA)
- General Health & Medical Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Priority Applications (13)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9302152A SE501439C2 (sv) | 1993-06-22 | 1993-06-22 | Sätt och anordning för analys av polynukleotidsekvenser |
| ES94921363T ES2141828T3 (es) | 1993-06-22 | 1994-06-22 | Acceso a analisis de secuencias de acidos nucleicos por extension de cebadores en paralelo. |
| AT94921363T ATE185843T1 (de) | 1993-06-22 | 1994-06-22 | Nukleinsäure-sequenzanalyse durch die methode der parallelen primerextension |
| EP99200676A EP0969103A3 (de) | 1993-06-22 | 1994-06-22 | Nukleinsäure-Sequenzanalyse durch die Methode der parallelen Primerextension |
| JP50308095A JP3722833B2 (ja) | 1993-06-22 | 1994-06-22 | 核酸配列分析の平行プライマー拡張手法 |
| US08/564,100 US6153379A (en) | 1993-06-22 | 1994-06-22 | Parallel primer extension approach to nucleic acid sequence analysis |
| CA002164715A CA2164715A1 (en) | 1993-06-22 | 1994-06-22 | Parallel primer extension approach to nucleic acid sequence analysis |
| AU72121/94A AU698553B2 (en) | 1993-06-22 | 1994-06-22 | Parallel primer extension approach to nucleic acid sequence analysis |
| EP94921363A EP0705349B1 (de) | 1993-06-22 | 1994-06-22 | NUKLEINSäURE-SEQUENZANALYSE DURCH DIE METHODE DER PARALLELEN PRIMEREXTENSION |
| DE69421277T DE69421277T2 (de) | 1993-06-22 | 1994-06-22 | NUKLEINSäURE-SEQUENZANALYSE DURCH DIE METHODE DER PARALLELEN PRIMEREXTENSION |
| PCT/US1994/007086 WO1995000669A1 (en) | 1993-06-22 | 1994-06-22 | Parallel primer extension approach to nucleic acid sequence analysis |
| US09/711,476 US7001722B1 (en) | 1993-06-22 | 2000-11-13 | Parallel primer extension approach to nucleic acid sequence analysis |
| US10/254,828 US20030082613A1 (en) | 1993-06-22 | 2002-09-25 | Parallel primer extension approach to nucleic acid sequence analysis |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| SE9302152A SE501439C2 (sv) | 1993-06-22 | 1993-06-22 | Sätt och anordning för analys av polynukleotidsekvenser |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| SE9302152D0 SE9302152D0 (sv) | 1993-06-22 |
| SE9302152L SE9302152L (sv) | 1994-12-23 |
| SE501439C2 true SE501439C2 (sv) | 1995-02-13 |
Family
ID=20390371
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SE9302152A SE501439C2 (sv) | 1993-06-22 | 1993-06-22 | Sätt och anordning för analys av polynukleotidsekvenser |
Country Status (9)
| Country | Link |
|---|---|
| EP (2) | EP0969103A3 (de) |
| JP (1) | JP3722833B2 (de) |
| AT (1) | ATE185843T1 (de) |
| AU (1) | AU698553B2 (de) |
| CA (1) | CA2164715A1 (de) |
| DE (1) | DE69421277T2 (de) |
| ES (1) | ES2141828T3 (de) |
| SE (1) | SE501439C2 (de) |
| WO (1) | WO1995000669A1 (de) |
Families Citing this family (90)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH07203998A (ja) * | 1994-01-26 | 1995-08-08 | Hamamatsu Photonics Kk | 核酸塩基配列決定方法 |
| GB9507238D0 (en) | 1995-04-07 | 1995-05-31 | Isis Innovation | Detecting dna sequence variations |
| US5763175A (en) * | 1995-11-17 | 1998-06-09 | Lynx Therapeutics, Inc. | Simultaneous sequencing of tagged polynucleotides |
| WO1997035033A1 (en) | 1996-03-19 | 1997-09-25 | Molecular Tool, Inc. | Method for determining the nucleotide sequence of a polynucleotide |
| US6143529A (en) * | 1996-08-14 | 2000-11-07 | Exact Laboratories, Inc. | Methods for improving sensitivity and specificity of screening assays |
| US6096273A (en) | 1996-11-05 | 2000-08-01 | Clinical Micro Sensors | Electrodes linked via conductive oligomers to nucleic acids |
| US5888778A (en) * | 1997-06-16 | 1999-03-30 | Exact Laboratories, Inc. | High-throughput screening method for identification of genetic mutations or disease-causing microorganisms using segmented primers |
| US6566101B1 (en) | 1997-06-16 | 2003-05-20 | Anthony P. Shuber | Primer extension methods for detecting nucleic acids |
| JP4789294B2 (ja) * | 1997-08-22 | 2011-10-12 | ソレクサ・インコーポレイテッド | ローリングプライマーを用いたｄｎａ伸長および分析 |
| US6322968B1 (en) | 1997-11-21 | 2001-11-27 | Orchid Biosciences, Inc. | De novo or “universal” sequencing array |
| WO1999047535A1 (en) | 1998-03-16 | 1999-09-23 | Millennium Pharmaceuticals, Inc. | METHODS AND COMPOSITIONS FOR DIAGNOSING AND TREATING CHROMOSOME-18p RELATED DISORDERS |
| US6872521B1 (en) | 1998-06-16 | 2005-03-29 | Beckman Coulter, Inc. | Polymerase signaling assay |
| US6245507B1 (en) | 1998-08-18 | 2001-06-12 | Orchid Biosciences, Inc. | In-line complete hyperspectral fluorescent imaging of nucleic acid molecules |
| US6150105A (en) * | 1998-08-20 | 2000-11-21 | Genetic Assays, Inc. | Methods of screening nucleic acids for nucleotide variations |
| US6964846B1 (en) | 1999-04-09 | 2005-11-15 | Exact Sciences Corporation | Methods for detecting nucleic acids indicative of cancer |
| EP1218541B1 (de) | 1999-07-26 | 2008-12-10 | Clinical Micro Sensors, Inc. | Nukelinsäuresequenzbestimmung mittels elektronischem nachweis |
| US6528319B1 (en) | 1999-09-02 | 2003-03-04 | Amersham Biosciences Corp | Method for anchoring oligonucleotides to a substrate |
| US6586177B1 (en) | 1999-09-08 | 2003-07-01 | Exact Sciences Corporation | Methods for disease detection |
| US6849403B1 (en) | 1999-09-08 | 2005-02-01 | Exact Sciences Corporation | Apparatus and method for drug screening |
| US6448010B1 (en) * | 1999-10-06 | 2002-09-10 | Amersham Pharmacia Biotech, Inc. | Method for detecting mutations using arrayed primer extension |
| US6596483B1 (en) | 1999-11-12 | 2003-07-22 | Motorola, Inc. | System and method for detecting molecules using an active pixel sensor |
| US6919174B1 (en) | 1999-12-07 | 2005-07-19 | Exact Sciences Corporation | Methods for disease detection |
| DE60043896D1 (de) | 1999-12-07 | 2010-04-08 | Exact Sciences Corp | Verfahren zum nachweis von lungenneoplasmen in fäkalen proben |
| DE10010282B4 (de) | 2000-02-25 | 2006-11-16 | Epigenomics Ag | Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben |
| DE10010280B4 (de) | 2000-02-25 | 2006-08-10 | Epigenomics Ag | Verfahren zur Detektion von Cytosin-Methylierung in DNA Proben |
| AU4099001A (en) * | 2000-03-13 | 2001-09-24 | Genset | Nucleic acid detection method and system |
| US6376191B1 (en) * | 2000-03-22 | 2002-04-23 | Mergen, Ltd. | Microarray-based analysis of polynucleotide sequence variations |
| AU2001254794A1 (en) | 2000-04-06 | 2001-10-23 | Epigenomics Ag | Diagnosis of diseases associated with dna adducts |
| EP1191036A3 (de) | 2000-08-25 | 2002-07-03 | Pfizer Products Inc. | Methoden und Verbindungen zur Diagnose und Behandlung von Krankheiten, an denen Angiogenese beteiligt ist |
| AU2001216942A1 (en) * | 2000-11-24 | 2002-06-03 | Asper Ou | Method of optimising the sequences of synthetic nucleic acids |
| GB0105790D0 (en) * | 2001-03-08 | 2001-04-25 | Expresson Biosystems Ltd | Detecting binding of mRNA to an oligonnucleotide array using RNA dependent nucleic acid modifying enzymes |
| WO2002079516A1 (en) * | 2001-03-30 | 2002-10-10 | Applera Corporation | Nucleic acid analysis using non-templated nucleotide addition |
| US6653082B2 (en) | 2001-05-17 | 2003-11-25 | Baylor College Of Medicine | Substrate-bound cleavage assay for nucleic acid analysis |
| EP2354157B1 (de) | 2001-08-14 | 2013-10-23 | Sentigen Biosciences, Inc. | Nukleinsäuren und Proteine von Insekten OR83B-Duftrezeptorgenen und Verwendungen davon |
| DE10154317B4 (de) | 2001-10-26 | 2005-06-09 | Epigenomics Ag | Verfahren zum Nachweis von Cytosin-Methylierungen in immobilisierten DNA Proben |
| EP1340818A1 (de) | 2002-02-27 | 2003-09-03 | Epigenomics AG | Verfahren und Nukleinsäuren zur Analyse von Kolonkrebszellen |
| DE10245145B4 (de) * | 2002-09-27 | 2004-12-02 | IPK-Institut für Pflanzengenetik und Kulturpflanzenforschung | Verfahren zum Nachweis von SNPs auf polydimensionalen Microarrays |
| CA2500255A1 (en) | 2002-10-01 | 2004-04-29 | Epigenomics Ag | Method and nucleic acids for the treatment of breast cell proliferative disorders |
| WO2005003304A2 (en) | 2003-06-20 | 2005-01-13 | Illumina, Inc. | Methods and compositions for whole genome amplification and genotyping |
| US20080064029A1 (en) | 2003-06-23 | 2008-03-13 | Epigenomics Ag | Methods and Nucleic Acids for Analyses of Colorectal Cell Proliferative Disorders |
| EP2354250A1 (de) | 2003-06-23 | 2011-08-10 | Epigenomics AG | Verfahren und Nukleinsäuren zur Analyse von Störungen der Proliferation kolorektaler Zellen |
| WO2005001140A2 (en) | 2003-06-23 | 2005-01-06 | Epigenomics Ag | Methods and nucleic acids for the analysis of colon cell proliferative disorders |
| ES2801379T3 (es) | 2003-12-01 | 2021-01-11 | Epigenomics Ag | Métodos y ácidos nucleicos para el análisis de la expresión génica asociada con el desarrollo de trastornos proliferativos de células de próstata |
| AU2004298537A1 (en) | 2003-12-11 | 2005-06-30 | Epigenomics Ag | Method and nucleic acids for the improved treatment of breast cell proliferative disorders |
| WO2006002344A1 (en) | 2004-06-23 | 2006-01-05 | Epigenomics Ag | Methods and nucleic acids for the detection of metastasis of colon cell proliferative disorders |
| EP2302073A1 (de) | 2004-07-18 | 2011-03-30 | Epigenomics AG | Epigenetische Verfahren und Nukleinsäuren für den Nachweis von proliferativen Erkrankungen von Mammazellen |
| EP1784508B1 (de) | 2004-08-09 | 2012-10-03 | Generation Biotech, LLC | Verfahren zur isolierung und amplifikation von nukleinsäuren |
| US9109256B2 (en) | 2004-10-27 | 2015-08-18 | Esoterix Genetic Laboratories, Llc | Method for monitoring disease progression or recurrence |
| EP2311984A1 (de) | 2004-12-02 | 2011-04-20 | Epigenomics AG | Verfahren und Nukleinsäuren zur Analyse von mit der Prognose von Störungen der Proliferation von Prostatazellen assoziierter Genexpression |
| EP1877577A2 (de) | 2005-04-15 | 2008-01-16 | Epigenomics Inc. | Verfahren und nukleinsäuren für analysen zellulärer proliferativer störungen |
| WO2007044071A2 (en) | 2005-04-21 | 2007-04-19 | Exact Sciences Corporation | Analysis of heterogeneous nucleic acid samples |
| WO2006119434A2 (en) | 2005-05-02 | 2006-11-09 | University Of Southern California | DNA METHYLATION MARKERS ASSOCIATED WITH THE CpG ISLAND METHYLATOR PHENOTYPE (CIMP) IN HUMAN COLORECTAL CANCER |
| US20090191548A1 (en) | 2005-09-29 | 2009-07-30 | Epigenomics Ag | Methods and nucleic acids for the analysis of gene expression associated with tissue classification |
| EP3095878A1 (de) | 2005-11-17 | 2016-11-23 | Epigenomics AG | Verfahren zur bestimmung des dna-methylierungsgrads einer cpg-position in identischen zellen in einer gewebeprobe |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| US5202231A (en) * | 1987-04-01 | 1993-04-13 | Drmanac Radoje T | Method of sequencing of genomes by hybridization of oligonucleotide probes |
| GB8810400D0 (en) * | 1988-05-03 | 1988-06-08 | Southern E | Analysing polynucleotide sequences |
| US5547839A (en) * | 1989-06-07 | 1996-08-20 | Affymax Technologies N.V. | Sequencing of surface immobilized polymers utilizing microflourescence detection |
| DE69132843T2 (de) * | 1990-12-06 | 2002-09-12 | Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) | Identifizierung von Nukleinsäuren in Proben |
| US6004744A (en) * | 1991-03-05 | 1999-12-21 | Molecular Tool, Inc. | Method for determining nucleotide identity through extension of immobilized primer |
-
1993
- 1993-06-22 SE SE9302152A patent/SE501439C2/sv not_active IP Right Cessation
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1994
- 1994-06-22 WO PCT/US1994/007086 patent/WO1995000669A1/en not_active Ceased
- 1994-06-22 EP EP99200676A patent/EP0969103A3/de not_active Withdrawn
- 1994-06-22 EP EP94921363A patent/EP0705349B1/de not_active Expired - Lifetime
- 1994-06-22 AT AT94921363T patent/ATE185843T1/de not_active IP Right Cessation
- 1994-06-22 CA CA002164715A patent/CA2164715A1/en not_active Abandoned
- 1994-06-22 DE DE69421277T patent/DE69421277T2/de not_active Expired - Fee Related
- 1994-06-22 AU AU72121/94A patent/AU698553B2/en not_active Ceased
- 1994-06-22 JP JP50308095A patent/JP3722833B2/ja not_active Expired - Fee Related
- 1994-06-22 ES ES94921363T patent/ES2141828T3/es not_active Expired - Lifetime
Also Published As
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|---|---|
| EP0969103A3 (de) | 2004-01-02 |
| JPH09501312A (ja) | 1997-02-10 |
| EP0705349A1 (de) | 1996-04-10 |
| SE9302152L (sv) | 1994-12-23 |
| AU698553B2 (en) | 1998-10-29 |
| SE9302152D0 (sv) | 1993-06-22 |
| JP3722833B2 (ja) | 2005-11-30 |
| CA2164715A1 (en) | 1995-01-05 |
| EP0705349B1 (de) | 1999-10-20 |
| EP0969103A2 (de) | 2000-01-05 |
| AU7212194A (en) | 1995-01-17 |
| DE69421277T2 (de) | 2000-05-31 |
| ATE185843T1 (de) | 1999-11-15 |
| ES2141828T3 (es) | 2000-04-01 |
| DE69421277D1 (de) | 1999-11-25 |
| WO1995000669A1 (en) | 1995-01-05 |
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