RU98117870A - Способ очистки тромбиноподобных протеаз из змеиного яда - Google Patents
Способ очистки тромбиноподобных протеаз из змеиного ядаInfo
- Publication number
- RU98117870A RU98117870A RU98117870/13A RU98117870A RU98117870A RU 98117870 A RU98117870 A RU 98117870A RU 98117870/13 A RU98117870/13 A RU 98117870/13A RU 98117870 A RU98117870 A RU 98117870A RU 98117870 A RU98117870 A RU 98117870A
- Authority
- RU
- Russia
- Prior art keywords
- chromatography
- thrombin
- glass
- proteases
- cleaning
- Prior art date
Links
- 108091005771 Peptidases Proteins 0.000 title claims 3
- 239000004365 Protease Substances 0.000 title claims 3
- 230000002345 thrombinlike Effects 0.000 title claims 3
- 102000035443 Peptidases Human genes 0.000 title 1
- 238000004140 cleaning Methods 0.000 title 1
- 239000011521 glass Substances 0.000 claims 4
- 238000004587 chromatography analysis Methods 0.000 claims 3
- 238000005227 gel permeation chromatography Methods 0.000 claims 3
- 238000000746 purification Methods 0.000 claims 3
- 102000033147 ERVK-25 Human genes 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 1
- 108090000790 Enzymes Proteins 0.000 claims 1
- 230000002378 acidificating Effects 0.000 claims 1
- 238000001042 affinity chromatography Methods 0.000 claims 1
- 150000001768 cations Chemical class 0.000 claims 1
- 150000002500 ions Chemical class 0.000 claims 1
- 238000000926 separation method Methods 0.000 claims 1
- 239000003998 snake venom Substances 0.000 claims 1
Claims (4)
1. Способ очистки тромбиноподобной протеазы из змеиною яда, отличающийся тем, что а) протеазу в виде сырого продукта подвергают предварительной очистке с помощью афинной хроматографии или хроматографии на основных ионообменниках, б) полученную таким образом фракцию с тромбиноподобными энзимами подвергают хроматографии на слабых катионообменниках или разделяют с помощью адсорбции на стекле в основной области и в) основную компоненту со стадии б) подвергают гель-хроматографии или очищают с помощью хроматографии на стекле в кислой области, причем, по меньшей мере одна из стадий б) или в), включает разделение с помощью хроматографии на стекле.
2. Способ по п. 1, отличающийся тем, что на стадии б) очистку проводят с помощью адсорбции на стекле, и стадию в) посредством гель - хроматографии.
3. Способ по п. 1, отличающийся тем, что очистку на стадиях б) и в) проводят с помощью гель - хроматографии.
4. Анкрод, который имеет чистоту выше 95%.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19607210A DE19607210A1 (de) | 1996-02-26 | 1996-02-26 | Verfahren zur Reinigung von thrombinähnlichen Proteasen aus Schlangengiften |
| DE19607210.7 | 1996-02-26 |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU98117870A true RU98117870A (ru) | 2000-09-10 |
| RU2186849C2 RU2186849C2 (ru) | 2002-08-10 |
Family
ID=7786490
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU98117870/13A RU2186849C2 (ru) | 1996-02-26 | 1997-02-18 | Способ очистки тромбиноподобной протеазы из змеиного яда |
Country Status (32)
| Country | Link |
|---|---|
| US (1) | US6200791B1 (ru) |
| EP (1) | EP0883684B1 (ru) |
| JP (1) | JP3817269B2 (ru) |
| KR (1) | KR100493835B1 (ru) |
| CN (1) | CN1188517C (ru) |
| AR (1) | AR005995A1 (ru) |
| AT (1) | ATE283350T1 (ru) |
| AU (1) | AU711650B2 (ru) |
| BG (1) | BG64875B1 (ru) |
| BR (1) | BR9707738A (ru) |
| CO (1) | CO4771158A1 (ru) |
| CZ (1) | CZ292482B6 (ru) |
| DE (2) | DE19607210A1 (ru) |
| ES (1) | ES2233997T3 (ru) |
| HK (1) | HK1017380A1 (ru) |
| HR (1) | HRP970108B1 (ru) |
| HU (1) | HU225489B1 (ru) |
| ID (1) | ID16046A (ru) |
| IL (1) | IL125395A (ru) |
| MX (1) | MX9806304A (ru) |
| MY (1) | MY130458A (ru) |
| NO (1) | NO319561B1 (ru) |
| NZ (1) | NZ331120A (ru) |
| PL (1) | PL186211B1 (ru) |
| PT (1) | PT883684E (ru) |
| RU (1) | RU2186849C2 (ru) |
| SK (1) | SK284874B6 (ru) |
| TR (1) | TR199801668T2 (ru) |
| TW (1) | TW505696B (ru) |
| UA (1) | UA46831C2 (ru) |
| WO (1) | WO1997032015A1 (ru) |
| ZA (1) | ZA971597B (ru) |
Families Citing this family (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1102173C (zh) * | 1999-03-26 | 2003-02-26 | 福建医科大学 | 蕲蛇酶及其生产工艺 |
| US20030219431A1 (en) * | 2002-05-24 | 2003-11-27 | Empire Pharmaceuticals, Inc. | Treatment of neuronal and neurological damage associated with spinal cord injury |
| CN101489655A (zh) | 2006-07-14 | 2009-07-22 | 威斯康星旧生研究基金会 | 捕获病毒用吸附膜 |
| CN103160486A (zh) * | 2013-04-02 | 2013-06-19 | 黑龙江迪龙制药有限公司 | 一种猪凝血酶的制备方法 |
| CN109929020A (zh) * | 2017-12-15 | 2019-06-25 | 浙江京新药业股份有限公司 | 一种眼镜蛇毒的纯化方法及其产品 |
| CN108559740B (zh) * | 2018-05-12 | 2021-05-18 | 吉林天衡康泰生物技术有限公司 | 重组安克洛酶及工业规模制备方法和治疗急性脑梗的应用 |
| CN109652398B (zh) * | 2018-12-29 | 2021-07-20 | 上海太阳生物技术有限公司 | 凝血因子ⅹ激活剂rvv-ⅹ的制备方法及制得的rvv-ⅹ |
| CN110016471B (zh) * | 2019-04-10 | 2020-10-02 | 北京博康宁生物医药科技有限公司 | 重组安克洛酶及工业规模制备和纯化方法及其组合物 |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB1177506A (en) | 1964-02-21 | 1970-01-14 | Nat Res Dev | Improvements relating to Anticoagulants. |
| GB1094301A (en) * | 1964-02-21 | 1967-12-06 | Nat Res Dev | Improvements relating to anticoagulants |
| GB1293793A (en) | 1969-04-28 | 1972-10-25 | Chrysler United Kingdom Ltd Fo | Improvements in or relating to valves |
| US3743722A (en) * | 1971-07-14 | 1973-07-03 | Abbott Lab | Anti-coagulant isolation |
| US3879369A (en) | 1972-05-12 | 1975-04-22 | Abbott Lab | Anti-coagulant isolation from malayan pit viper using affinity chromatography |
| GB1472574A (en) | 1973-09-03 | 1977-05-04 | Biochimici Fargal Pharmasint S | Process for the extraction of components having anticoagulant activity -in vivo- from snake venoms and related products |
| NL7605805A (nl) * | 1976-05-28 | 1977-11-30 | Stichting Rega V Z W | Werkwijze voor het zuiveren van interferon. |
| CH626917A5 (ru) | 1976-08-17 | 1981-12-15 | Pentapharm Ag | |
| JPS5569595A (en) * | 1978-11-20 | 1980-05-26 | Suguru Abe | Novel substance having fibrinolytic activity, remedy for thrombotic embolic disease comprising it, and its preparation |
| EP0328256A1 (en) * | 1988-01-21 | 1989-08-16 | Owens-Corning Fiberglas Corporation | Glass fibers coated with agarose for use as column packing or chromatographic media for bioseparations |
| US5712117A (en) * | 1995-02-08 | 1998-01-27 | Zymogenetics, Inc. | Cytoplasmic antiproteinase-2 and coding sequences |
-
1996
- 1996-02-26 DE DE19607210A patent/DE19607210A1/de not_active Withdrawn
-
1997
- 1997-02-18 EP EP97903312A patent/EP0883684B1/de not_active Expired - Lifetime
- 1997-02-18 PT PT97903312T patent/PT883684E/pt unknown
- 1997-02-18 UA UA98094992A patent/UA46831C2/uk unknown
- 1997-02-18 PL PL97328568A patent/PL186211B1/pl not_active IP Right Cessation
- 1997-02-18 ES ES97903312T patent/ES2233997T3/es not_active Expired - Lifetime
- 1997-02-18 MX MX9806304A patent/MX9806304A/es unknown
- 1997-02-18 AU AU17913/97A patent/AU711650B2/en not_active Ceased
- 1997-02-18 HU HU9900505A patent/HU225489B1/hu not_active IP Right Cessation
- 1997-02-18 KR KR10-1998-0706648A patent/KR100493835B1/ko not_active IP Right Cessation
- 1997-02-18 WO PCT/EP1997/000755 patent/WO1997032015A1/de active IP Right Grant
- 1997-02-18 US US09/125,374 patent/US6200791B1/en not_active Expired - Lifetime
- 1997-02-18 AT AT97903312T patent/ATE283350T1/de active
- 1997-02-18 CZ CZ19982697A patent/CZ292482B6/cs not_active IP Right Cessation
- 1997-02-18 IL IL12539597A patent/IL125395A/en not_active IP Right Cessation
- 1997-02-18 RU RU98117870/13A patent/RU2186849C2/ru active
- 1997-02-18 NZ NZ331120A patent/NZ331120A/xx not_active IP Right Cessation
- 1997-02-18 SK SK1132-98A patent/SK284874B6/sk not_active IP Right Cessation
- 1997-02-18 JP JP53055397A patent/JP3817269B2/ja not_active Expired - Lifetime
- 1997-02-18 CN CNB971925852A patent/CN1188517C/zh not_active Expired - Lifetime
- 1997-02-18 TR TR1998/01668T patent/TR199801668T2/xx unknown
- 1997-02-18 DE DE59712093T patent/DE59712093D1/de not_active Expired - Lifetime
- 1997-02-18 BR BR9707738A patent/BR9707738A/pt not_active Application Discontinuation
- 1997-02-21 TW TW086102111A patent/TW505696B/zh not_active IP Right Cessation
- 1997-02-24 ID IDP970561A patent/ID16046A/id unknown
- 1997-02-24 MY MYPI97000700A patent/MY130458A/en unknown
- 1997-02-25 CO CO97010013A patent/CO4771158A1/es unknown
- 1997-02-25 AR ARP970100755A patent/AR005995A1/es active IP Right Grant
- 1997-02-25 ZA ZA971597A patent/ZA971597B/xx unknown
- 1997-02-26 HR HR970108A patent/HRP970108B1/xx not_active IP Right Cessation
-
1998
- 1998-07-31 BG BG102663A patent/BG64875B1/bg unknown
- 1998-08-25 NO NO19983893A patent/NO319561B1/no not_active IP Right Cessation
-
1999
- 1999-06-10 HK HK99102524A patent/HK1017380A1/xx not_active IP Right Cessation
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