CA2247016A1 - Purification of thrombin-like proteases from snake venoms - Google Patents

Purification of thrombin-like proteases from snake venoms

Info

Publication number
CA2247016A1
CA2247016A1 CA002247016A CA2247016A CA2247016A1 CA 2247016 A1 CA2247016 A1 CA 2247016A1 CA 002247016 A CA002247016 A CA 002247016A CA 2247016 A CA2247016 A CA 2247016A CA 2247016 A1 CA2247016 A1 CA 2247016A1
Authority
CA
Canada
Prior art keywords
thrombin
proteases
purification
snake venoms
snake
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
CA002247016A
Other languages
French (fr)
Other versions
CA2247016C (en
Inventor
Margarete Schwarz
Wolfgang Zahn
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
NORDMARK HOLDING GmbH
Original Assignee
Individual
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE19607210A external-priority patent/DE19607210A1/en
Application filed by Individual filed Critical Individual
Publication of CA2247016A1 publication Critical patent/CA2247016A1/en
Application granted granted Critical
Publication of CA2247016C publication Critical patent/CA2247016C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/64—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue
    • C12N9/6402—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals
    • C12N9/6418—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from animal tissue from non-mammals from snakes

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

Described is a method of purifying thrombin-like protease enzymes from snake venom, the method consisting of freeing the enzymes of impurities in three chromatographic stages.
CA002247016A 1996-02-26 1997-02-18 Purification of thrombin-like proteases from snake venoms Expired - Lifetime CA2247016C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE19607210.7 1996-02-26
DE19607210A DE19607210A1 (en) 1996-02-26 1996-02-26 Process for the purification of thrombin-like proteases from snake venoms
PCT/EP1997/000755 WO1997032015A1 (en) 1996-02-26 1997-02-18 Method of purifying thrombin-like protease enzymes obtained from snake venom

Publications (2)

Publication Number Publication Date
CA2247016A1 true CA2247016A1 (en) 1997-09-04
CA2247016C CA2247016C (en) 2006-10-24

Family

ID=37387461

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002247016A Expired - Lifetime CA2247016C (en) 1996-02-26 1997-02-18 Purification of thrombin-like proteases from snake venoms

Country Status (1)

Country Link
CA (1) CA2247016C (en)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113804770A (en) * 2020-06-16 2021-12-17 中国人民解放军海军特色医学中心 Quality control method of sea snake venom and application thereof

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN113804770A (en) * 2020-06-16 2021-12-17 中国人民解放军海军特色医学中心 Quality control method of sea snake venom and application thereof

Also Published As

Publication number Publication date
CA2247016C (en) 2006-10-24

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Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20170220