RU96101052A - Способ получения пировиноградной кислоты - Google Patents
Способ получения пировиноградной кислотыInfo
- Publication number
- RU96101052A RU96101052A RU96101052/13A RU96101052A RU96101052A RU 96101052 A RU96101052 A RU 96101052A RU 96101052/13 A RU96101052/13 A RU 96101052/13A RU 96101052 A RU96101052 A RU 96101052A RU 96101052 A RU96101052 A RU 96101052A
- Authority
- RU
- Russia
- Prior art keywords
- catalase
- acid
- unit
- glycolate oxidase
- approximately
- Prior art date
Links
- 239000002253 acid Substances 0.000 title 1
- 238000004519 manufacturing process Methods 0.000 title 1
- JVTAAEKCZFNVCJ-REOHCLBHSA-N L-lactic acid Chemical compound C[C@H](O)C(O)=O JVTAAEKCZFNVCJ-REOHCLBHSA-N 0.000 claims 6
- LCTONWCANYUPML-UHFFFAOYSA-N pyruvic acid Chemical compound CC(=O)C(O)=O LCTONWCANYUPML-UHFFFAOYSA-N 0.000 claims 6
- 229940105657 CATALASE Drugs 0.000 claims 4
- 102000016938 EC 1.11.1.6 Human genes 0.000 claims 4
- 108010053835 EC 1.11.1.6 Proteins 0.000 claims 4
- 102100005453 HAO2 Human genes 0.000 claims 4
- 108010062584 glycollate oxidase Proteins 0.000 claims 4
- 229940107700 Pyruvic Acid Drugs 0.000 claims 3
- 108090000790 Enzymes Proteins 0.000 claims 2
- 102000004190 Enzymes Human genes 0.000 claims 2
- 239000003054 catalyst Substances 0.000 claims 2
- 230000003197 catalytic Effects 0.000 claims 2
- 230000024881 catalytic activity Effects 0.000 claims 2
- 229940088598 Enzyme Drugs 0.000 claims 1
- 239000007864 aqueous solution Substances 0.000 claims 1
- 239000011942 biocatalyst Substances 0.000 claims 1
- 230000000694 effects Effects 0.000 claims 1
- 230000003993 interaction Effects 0.000 claims 1
- 238000002955 isolation Methods 0.000 claims 1
- 230000000813 microbial Effects 0.000 claims 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N oxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 claims 1
- 229910052760 oxygen Inorganic materials 0.000 claims 1
- 239000001301 oxygen Substances 0.000 claims 1
Claims (5)
1. Способ получения пировиноградной кислоты, отличающийся тем, что он включает стадии взаимодействия в водном растворе L-молочной кислоты с кислородом в присутствии гликолатоксидазы и каталазы в качестве катализатора в течение времени, достаточного для превращения L-молочной кислоты в пировиноградную кислоту с высокими ее выходами, в котором исходная концентрация L-молочной кислоты составляет примерно 0,1 - 2,0 М и последующего выделения пировиноградной кислоты.
2. Способ по п.1, отличающийся тем, что указанные ферментные катализаторы (биокатализаторы) используют в виде микробной клетки-трансформанта.
3. Способ по п.1, отличающийся тем, что реакцию взаимодействия осуществляют без введения дополнительного буфера и pH регулирования.
4. Способ по п.1, отличающийся тем, что реакцию проводят при pH в интервале примерно 6 - 10, и каталитическая активность по гликолатоксидазе составляет примерно 0,01 - 1000 М.ед/мл, а по каталазе примерно 0,01 - 10 М. ед/мл.
5. Способ по п. 3, отличающийся тем, что каталитическая активность по гликолатоксидазе составляет примерно 0,1 - 10 М.ед/мл, и по каталазе примерно 2000 - 15000 М.ед/мл, а соотношение М.ед. активности /мл каталазы к гликолатоксидазе составляет по меньшей мере 250 : 1.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US8287993A | 1993-06-25 | 1993-06-25 | |
| US08/082,879 | 1993-06-25 | ||
| PCT/US1994/006436 WO1995000656A1 (en) | 1993-06-25 | 1994-06-15 | Process for the preparation of pyruvic acid |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| RU2123529C1 RU2123529C1 (ru) | 1998-12-20 |
| RU96101052A true RU96101052A (ru) | 1998-12-27 |
Family
ID=22174039
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| RU96101052A RU2123529C1 (ru) | 1993-06-25 | 1994-06-15 | Способ получения пировиноградной кислоты |
Country Status (15)
| Country | Link |
|---|---|
| EP (1) | EP0705345B1 (ru) |
| JP (1) | JP3709202B2 (ru) |
| KR (1) | KR100274552B1 (ru) |
| CN (1) | CN1096500C (ru) |
| AU (1) | AU686182B2 (ru) |
| BR (1) | BR9407269A (ru) |
| CA (1) | CA2165940C (ru) |
| DE (1) | DE69417892T2 (ru) |
| ES (1) | ES2132409T3 (ru) |
| GR (1) | GR3030756T3 (ru) |
| HU (1) | HU213759B (ru) |
| NZ (1) | NZ267894A (ru) |
| RU (1) | RU2123529C1 (ru) |
| WO (1) | WO1995000656A1 (ru) |
| ZA (1) | ZA944559B (ru) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5869301A (en) * | 1995-11-02 | 1999-02-09 | Lockhead Martin Energy Research Corporation | Method for the production of dicarboxylic acids |
| JP4367616B2 (ja) | 2003-10-21 | 2009-11-18 | 日本電気株式会社 | 耐熱性乳酸酸化酵素 |
| US7470813B2 (en) | 2007-03-30 | 2008-12-30 | Ge Healthcare Limited | Method for the production of pyruvic acid |
| CN106591251B (zh) * | 2017-01-05 | 2020-01-03 | 江南大学 | 一种氧化酶及其应用 |
| CN106754796B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106754785B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106754783B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106754786B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106701706B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106591252B (zh) * | 2017-01-05 | 2019-11-26 | 江南大学 | 一种氧化酶及其应用 |
| CN106754784B (zh) * | 2017-01-05 | 2019-11-26 | 江南大学 | 一种氧化酶及其应用 |
| CN106701703B (zh) * | 2017-01-05 | 2019-11-08 | 江南大学 | 一种氧化酶及其应用 |
| CN106754799B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106636022B (zh) * | 2017-01-05 | 2020-01-07 | 江南大学 | 一种氧化酶及其应用 |
| CN106754795B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106754778B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106754787B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106754798B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106754779B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106754780B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN106754797B (zh) * | 2017-01-05 | 2019-11-15 | 江南大学 | 一种氧化酶及其应用 |
| CN109988784B (zh) * | 2019-04-16 | 2021-02-02 | 台州学院 | 一种固定化甘醇酸氧化酶催化合成丙酮酸的方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE3733157A1 (de) * | 1987-10-01 | 1989-04-27 | Basf Ag | Verfahren zur herstellung von brenztraubensaeure |
| JP2804079B2 (ja) * | 1988-05-18 | 1998-09-24 | 協和メデックス株式会社 | Ｎａｄ（ｐ）ｈの定量法 |
-
1994
- 1994-06-15 JP JP50286895A patent/JP3709202B2/ja not_active Expired - Fee Related
- 1994-06-15 WO PCT/US1994/006436 patent/WO1995000656A1/en active IP Right Grant
- 1994-06-15 EP EP94919409A patent/EP0705345B1/en not_active Expired - Lifetime
- 1994-06-15 HU HU9503773A patent/HU213759B/hu not_active IP Right Cessation
- 1994-06-15 RU RU96101052A patent/RU2123529C1/ru not_active IP Right Cessation
- 1994-06-15 DE DE69417892T patent/DE69417892T2/de not_active Expired - Fee Related
- 1994-06-15 CA CA002165940A patent/CA2165940C/en not_active Expired - Fee Related
- 1994-06-15 NZ NZ267894A patent/NZ267894A/en unknown
- 1994-06-15 ES ES94919409T patent/ES2132409T3/es not_active Expired - Lifetime
- 1994-06-15 CN CN94192574A patent/CN1096500C/zh not_active Expired - Fee Related
- 1994-06-15 KR KR1019950705919A patent/KR100274552B1/ko not_active IP Right Cessation
- 1994-06-15 AU AU70567/94A patent/AU686182B2/en not_active Ceased
- 1994-06-15 BR BR9407269A patent/BR9407269A/pt not_active IP Right Cessation
- 1994-06-24 ZA ZA944559A patent/ZA944559B/xx unknown
-
1999
- 1999-07-14 GR GR990401841T patent/GR3030756T3/el unknown
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