RU2743879C1 - Method for prevention of udder inflammation in cows in interlactation period and after delivery - Google Patents

Abstract

FIELD: veterinary medicine; medicine.SUBSTANCE: invention refers to veterinary medicine, namely to methods for prevention of mastitis in cows in interlactation period and after childbirth. Essence of development consists in address skin application to cows initially after last milking in start and repeatedly 15 days before calving, on skin of udder share, in which in the lactation period there was inflammation, an antimicrobial preparation with penetrating properties in dose of 30.0 g and with skin treatment of the corresponding teat dug in amount of 1.0–1.5 g, is characterized by that as an antimicrobial agent with penetrating properties, linseed intrasan (100 g contains as active substances 10.0 g of dimethylsulphoxide and 3.0 g of potassium iodide, and as auxiliary substances sodium thiosulphate - 0.05 g, polyvinylpyrrolidone - 0.3 g, solid fat type A - 30.0 g, emulsifier No 1 (Lanette SX) - 7.0 g and distilled water to 100.0 g).EFFECT: method provides more effective prevention of mastitis in cows in interlactation period and postpartum, as well as quality of milk.1 cl, 4 tbl

Description

The invention relates to veterinary medicine, namely to methods for the prevention of mastitis in cows during the dry period and after childbirth.

In the system of preventive measures for mastitis in cows, an important role is played by the dry period. In dry cows, the mammary gland has two critical periods associated with involutional processes, these are the first 10-15 days after starting and 10-15 days before delivery. During these periods, an inflammatory process in the udder and frequent recurrence of the pathological process that took place during lactation occurs most often. One of the methods of preventing mastitis during the dry period and after calving is the introduction of prolonged imported antimicrobial drugs into all the lobes of the udder after the last milking during the start of the cow. In countries with developed dairy cattle breeding, drugs with the DC designation are widely popular. However, their bulk consists of antibiotics or their combinations [RU 2301663 C1, A61K 31/00, 27.06.2007; RU 2448705 C1, A61K 31/43, 27.04.2012 and others]. The use of antibacterial drugs leads to the formation of high drug resistance in strains of microorganisms circulating in the farm. Ultimately, more and more potent drugs are used for prevention. All of them, as well as the products of their metabolism, to one degree or another, are toxic and harmful to young animals and humans. For example, after heat treatment of animal products for consumption, the antibiotics contained in them acquire the properties of the strongest allergens. [Vasiliev V.G. Therapy of cows with mastitis // Veterinary medicine. - 1998. - No. 1. - S. 38-40; Golovko A.N., Vechtamov V.Ya., Guzhvinskaya S.A., Makeev V.F. Etiopathogenesis and therapy of mastitis in cows // Veterinary medicine. - 2001. - No. 11. - S. 37-39]. In addition, the drugs used and their metabolites, subsequently released with milk secretion and entering the body of the newborn, have an iatrogenic effect [Gariyon Zh.L. Quantum medicine in Russia and in the world - yesterday, today and tomorrow // Sat. tr. VII All-Russian scientific-practical. conf. on quantum therapy. - M .: JSC "Milta-PKPGIT", 2001]. And also antibiotics, sulfonamides, which are part of the drugs, irritate the breast tissue, inhibit local resistance, disrupt the natural biocenosis of the mammary gland, which leads to the development of dysbiosis. For a long time, the components of drugs (antibiotics, sulfonamides) are excreted in milk, which poses a threat to health (2).

In view of the above, some experts (VM Kartashova, Yu.N. Zabelin "Prevention of mastitis during the dry period". J. Veterinary, 1984. No. 1. P.48-49; S. Haasman et al. Zur Staphylocokken mastitis des Rundes Mh. Vet.-med. Berl. 1993. Ig.38. H.24. P.927-931) recommend limiting the use of antibiotic-containing preparations made according to the DC formula only on animals that have undergone mastitis during lactation.

Known adhesive compositions for protecting the udder of dry cows from the penetration of microorganisms from the external environment, such as furoplast. (Kartashova V.M., Zabelin Yu.A. Prevention of mastitis during the dry period. // Veterinary medicine - 1984 - No. 1 - P.48.) They did not receive practical use, apparently, due to the one-sided protective action - the nipple canal is protected from the external environment, which does not provide reliable prevention of mastitis in cows.

In addition, it is known to treat udder teats during the start-up period with a film-antiseptic coating for the prevention of mastitis during the dry period (Roman L.G. Film-antiseptic coating - a means of preventing mastitis in dry cows / L.G. Roman, N.I. Polyantsev // Scientific journal. Proceedings of the Kuban State Agrarian University. - Krasnodar. - 2008. - Issue No. 6 (15). - P.116-119.). Disadvantages: low therapeutic efficiency, high cost and labor intensity.

Therefore, at present, the main direction in preventive measures for mastitis in cows in the dry period should be the targeted use of agents that do not contain antibiotics and sulfonamides, do not irritate the parenchyma of the mammary gland, and do not have a negative effect on general and local resistance.

Closest to the proposed method is a method for the prevention of mastitis in cows during the dry period using a cream warming mastisept [1]. 1.0 g of this preparation contains 0.05 g of camphor, 0.02 g of methyl salicylate, 20 mg of black poplar leaf bud oil, a composition of liquid paraffins (E905) and food colors (E102; E131).

The disadvantages of this method of prevention of mastitis include: the presence of irritation of the tissues of the udder; the drug should be applied within a day 2-3 times; has antimicrobial properties only to S. aureus, Enteroccocus spp., S. agalactiae, Escherichia coli., which are located on the surface of the skin; does not have penetrating properties; there are restrictions on the slaughter of animals and the use of milk within 3 days after its last use.

The technical result is achieved by the fact that in the proposed method for the prevention of mastitis in cows during the dry period and after childbirth, including the targeted cutaneous application of an antimicrobial drug with penetrating properties, initially after the last milking on the skin of the udder lobe, in which there was inflammation during lactation, and again for 15 days before calving, intrasan liniment is used as an antimicrobial drug with penetrating properties (in 100 g it contains 10 g of dimethyl sulfoxide and 3 g of potassium iodide as active ingredients, and sodium thiosulfate as auxiliary substances - 0.05 g, polyvinylpyrrolidone - 0.3 g, solid fat type A - 30 g, emulsifier No. 1 (Lanette SX) - 7 g and distilled water up to 100 g) at a dose of 30.0 g over each lobe and with the treatment of the skin of the corresponding teat of the udder in the amount of 1.0 -1.5 g). In relation to the prototype, the active substances of Intrasan easily penetrate the skin of the udder deep into the organ, have an anti-inflammatory, decongestant, analgesic, antimicrobial effect on gram-positive and gram-negative microorganisms and spore microflora, normalize regenerative processes, potassium iodide also replenishes the iodine deficiency in the body and, in concentrations does not have a local irritant, allergenic and resorptive-toxic effect. When using Intrasan, there are no waiting times for the slaughter of animals and the use of milk from them. During the dry period, for prophylactic purposes, intrasan is applied to the udder with mastitis twice in the first day after starting and 15 days before the calving date, 30 g each with the treatment of the skin of the nipples of the corresponding lobes of the mammary gland (1.0-1.5 g) ...

Preliminarily experimentally, the optimal doses of Intrasan and the intervals of treatment of the lobes of the udder and the skin of their nipples in cows who had been ill during lactation and the skin of their teats were established during the dry period.

Microbiological studies have established that Intrasan has a bactericidal effect against gram-positive, gram-negative and spore microflora obtained from the udder of cows with mastitis during the dry period.

The effect of the cutaneous application of Intrasan on the dynamics of somatic cells (SC) in the udder secretion after start-up in cows was studied (Table 1).

It has been proved (Table 1) that 48 hours after a single application to the skin of a quarter of a patient with mastitis during lactation, the number of SC decreased by 22% and remained so after 72 hours.

Therefore, Intrasan does not irritate the udder parenchyma in dry cows.

It was shown in the experiment (Table 2) that 72 hours after the treatment of the udder skin with Intrasan in dry cows in the blood, the content of circulating immune complexes of small and medium sizes (C ₃ ) decreased by 24.2%, and in large ones by 60.2%. ... Against this background, the process of transformation of smaller CECs into larger analogs was actively taking place, and their own synthesis also intensified.

Consequently (Table 2) Intrasan has a stimulating effect on the opsonization of microorganisms that are latent in the diseased udder quarters of dry cows, and their active elimination through the synthesis of large circulating immune complexes, and their subsequent decrease in the blood indicates the neutralization of the existing antigens in the mammary gland, where immunoglobulins are also actively involved.

We studied the question of the effect of treatment of the skin of quarters of the mammary gland of cows with Intrasanum on the indices of endogenous intoxication of the cows' organism (Table 3).

It was found (Table 3) that after a single treatment of cows with Intrasanum, the content of toxic substances in the blood plasma after 24, 48 and 72 hours was, respectively, 50.0, 44.8 and 25.9% lower than the indicator before the experiment. In erythrocytes, this indicator decreased after 24 hours by 11.6%. The indicator of the intoxication criterion also testifies to a lower level of endotoxicosis after treatment of cows, the value of which in animals after 24, 48 and 72 hours decreased by 45.6%, 61.9% and 44.6%, respectively.

In order to determine the prophylactic efficacy, Intrasan was tested during the dry period on cows who had recovered from mastitis during lactation. For this, 2 groups of analogue cows were selected. The skin over the quarters of the udder in the cows of the experimental group was treated with intrasan, and in the animals of the control group with mastisept. The results of the experiment are presented in table 4.

It has been established (Table 4) that the number of skin applications of Intrasan is 2.8 times less compared to the analogue of comparison, which were applied at the same time - after the last milking at start-up and 15 days before calving. As a result of the tests, it was proved that the use of Intrasan according to the recommended method prevents mastitis in dry conditions in 95% of cases in cows and in 92.8% in udder lobes, which is, respectively, 20 and 11.4% higher than when using an analogue. In addition, Intrasan after childbirth prevents re-inflammation of the udder in 90% of cows and in 85.7% of treated breast quarters, which is respectively 35% and 8% more than mastissept. Based on the above data, a conclusion can be drawn about the higher preventive effectiveness of Intrasan. With the use of the recommended agent, there were 119 thousand / ml fewer somatic cells in milk at 10-11 days after calving in cows in comparison with the prototype.

Sources of information

1 INSTRUCTIONS for the use of the veterinary drug "MASTISEPT", prototype

2 Konopeltsev I.G., Shulyat'ev V.N. Inflammation of the udder in cows: a tutorial // Iz-vo SPB AVM. - 2010 .-- 355 p.

Claims (1)

A method for the prevention of udder inflammation in cows during the dry period and after childbirth, which consists in targeted skin application, initially after the last milking at start-up and again 15 days before calving, on the skin of the udder lobe, in which there was inflammation during lactation, of an antimicrobial drug with penetrating properties in dose of 30.0 g and with the treatment of the skin of the corresponding teat of the udder in an amount of 1.0-1.5 g, as an antimicrobial drug with penetrating properties, intrasan liniment is used, in 100 g contains as active ingredients: 10.0 g of dimethyl sulfoxide and 3.0 g of potassium iodide, and sodium thiosulfate as auxiliary substances - 0.05 g, polyvinylpyrrolidone - 0.3 g, solid fat type A - 30.0 g, emulsifier No. 1 Lanette SX - 7.0 g and distilled water up to 100.0 g.