RU2521401C1 - Agent for prevention of mastitis in dairy cows in dry period - Google Patents

Abstract

FIELD: veterinary medicine.

SUBSTANCE: agent for prevention of mastitis in dairy cows in the dry period, characterised in that it contains as ingredients the polymer PVP/VA, silicone, polyhexamethylene biguanide, ethyl alcohol, glycerol - as emollient component, gelation agents - triethanolamine and carbopol and purified water in the following ratio of ingredients, %: polymer PVP/VA 9-10; silicone 5-6; ethyl alcohol 35-40; polyhexamethylene biguanide 0.008-0.01; glycerol 5; gelation agents - triethanolamine and carbopol 0.1; purified water - the rest.

EFFECT: method enables to minimise or eliminate the use of chemotherapeutic agents, antibiotics and their derivatives, sulphonamides, nitrofurans and eliminate the side effect of the agent on animal body, as well as to reduce the number of visits of the veterinarian during the dry period of cows, to improve the preventive efficacy.

1 tbl, 3 ex

Description

The proposed tool relates to the field of veterinary medicine and is intended, in particular, for the pharmacoprophylaxis of mastitis in cows after transferring them to dead wood.

The mammary gland of dry cows is vulnerable to mastitis pathogens, and the peak incidence occurs, in particular, in the first 2-3 weeks after cessation of milking, i.e. on the degenerative phase of postlactational involution of the udder.

It is known to introduce in all lobes of the udder of cows immediately after milking the special dosage form of antibiotics, for example, nafpenza DC, orbenin DC (B. Boddle, S. Niskersson. Dry Cows therapy. I. Dairy Sci., 1996. No. 1. - P. 253-257). A single use of them provides protection of the udder from pathogens for 2-4 weeks.

In Western Europe and the USA, these funds are very popular among farmers, because thanks to the introduction of these funds, the cow stops milking, which greatly facilitates the launch of highly productive cows and reduces the number of visits by a veterinarian to conduct medical work on mastitis. However, their use in herds with an annual milk yield below 4500 kg and in herds with a classical system of launching (gradual cessation of milking) of cows does not justify the costs (OF Shakirov. One-time launch of cows with loose housing / J. Dairy and beef cattle breeding, 2005 . No. 7. S.35-38).

Known adhesive compositions for protecting the udder of dry cows from the penetration of microorganisms from the external environment, such as furoplast. (Kartashova V.M., Zabelin Yu.A. Prophylaxis of mastitis during the dead season. // Veterinary Medicine - 1984 - No. 1 - P.48.) They did not receive practical application, obviously, because of the one-sided protective effect - the nipple channel is protected from the external environment, which does not provide reliable prevention of mastitis in cows.

In addition, it is known to treat the nipples of the udder during the launch period with a film-antiseptic coating for the prevention of mastitis in the dry period (Roman L.G. Film-antiseptic coating - profilactiti mastitis agent for dry cows / L.G. Roman, N.I. Polyantsev // Scientific journal. Proceedings of the Kuban State Agrarian University. - Krasnodar. - 2008. - Issue No. 6 (15). - S.116-119.). Disadvantages: low therapeutic efficacy.

The closest analogue of the proposed tool is a film-antiseptic coating that contains iodismuthsulfamide and a biopolymer. The biopolymer is obtained by the interaction of urea, formalin, glycerol, followed by the introduction of a 50% solution of polyvinylpyrrolidone.

The disadvantages of this prototype include:

- processing of the nipples of the udder is performed repeatedly, mainly on the 1st and 8th day.

- the safety of the protective coating is small, the film on the nipple lasts no more than 5-7 days.

Tasks: To develop a means of preventing mastitis in cows during dead wood without the use of antibiotics and other chemotherapeutic agents leading to culling of milk, as well as to reduce the number of visits by a veterinarian to animals, to increase the prophylactic effect of mastitis in dry cows.

The essence of the proposed means of preventing mastitis in dairy cows during the dry season includes the use of a polymer film and differs in that this agent contains polyhexomethylene biguanide (0.008-0.01%) and ethyl alcohol (35-40%) as a antiseptic, and the film coating has a polymer (9-10%), for example, water-soluble polymer PVP / VA, silicone (5-6%) and glycerin (5%) as a softening component, and gelling agents - triethanolamine (0.1%) and carbopol (0.1 %). Water is included in the composition (39-46%) as a solvent and to ensure a sufficient gel consistency.

Technical result: the proposed method allows to exclude the use of chemotherapeutic agents, to exclude the use of antibiotics and their derivatives, sulfonamides, nitrofurans and thus eliminate the side effect of the drug on the animal organism, as well as reduce the number of visits by a veterinarian during dry cows and increase prophylactic effectiveness.

Broad-spectrum polyhexamethylene biguanide bactericidal agent. At relatively low concentrations, the bactericidal effect of the substance is bacteriostatic; at higher concentrations - sharply bactericidal. Ethyl alcohol has the properties of a disinfectant, anti-inflammatory, analgesic and distracting agent.

Water-soluble polymer and silicone provide the formation on the skin surface of a thin elastic film that insulates it from contact with the external environment.

Glycerin is able to hold a certain amount of water, protecting the skin from excessive moisture loss.

Triethanolamine and carbopol are used to give the product a gel-like consistency, thereby reducing the consumption of the product and its fixation on the nipples of the cow.

The claimed tool is obtained as follows:

According to the recipe, purified water is taken into the measuring container and the carbapole gel generator is added, for its uniform distribution in water, constant stirring is carried out for 40 minutes. After that, a polymer is introduced into a container with a mixture of carbopol and water, with constant stirring. Then, after thorough stirring, the resulting mixture was brought to a temperature of 40-45 ° C and glycerin, silicone and triethanolamine were added, mixed well and allowed to cool to room temperature. After cooling, alcohol, polyhexomethylene biguanide and purified water are added to the resulting mixture. After obtaining a mixture of all components, the resulting gel is thoroughly mixed.

The resulting product is used as follows: during start-up, immediately after the last milking, the udder's nipples are wiped with a dry cloth or towel and this product is applied using plastic cups, spending 2-3 g of the drug per animal, after which the cows are identified as dead wood.

A comparative assessment of the protective properties of the film coating. Of the cows with lactation, three equal groups were formed, each group included 50 goals: 1st — a single treatment of the skin of the nipples of the udder with the claimed agent; 2nd — double, with a weekly interval, treatment of the skin of the nipples of the udder with a film-antiseptic coating (PAP); 3rd - without the use of pharmacoprophylaxis.

In experimental animals of 3 groups, on the 15th day of the dry period and after calving, the mammary gland was examined using clinical methods and a test reaction, the results are shown in the table.

In the 3rd group (negative control) mastitis on the 15th day of the dry period was found in 13 animals, including nine clinically expressed and four subclinical. In the interval from 15 days to calving, another 4 cows fell ill. Thus, the total incidence was 34%.

Table Comparative analysis of the results of using the proposed means for the prevention of mastitis in cows during the dry period Group n Prophylactic Cows with mastitis have been identified. On the 15th day For the entire period of dead wood Clinical mastitis Subclinical mastitis Clinical mastitis Subclinical mastitis Qty % Qty % Qty % Qty % 1st fifty The claimed tool 0 0 2 four 3 6 2 four 2nd fifty Pap four 8 2 four 5 10 3 6 3rd fifty - 9 eighteen four 8 12 24 5 10

In the 2nd group, on the 15th day, mastitis was detected in six cows, and in four - clinically expressed and in two - subclinical. In the subsequent period, another 2 cows fell ill. As a result, the incidence is reduced to 16% or 2 times, compared with the control.

In the 1st group, mastitis on the 15th day was found in 2 cows, and only subclinical. A total of 5 cows with mastitis, three with a clinical and two with a subclinical, were identified during the dead wood period, which amounted to 10% of the livestock. It follows from this that when using the claimed agent, the prophylactic effect is 1.6 times higher compared to PAP.

Example 1. 300 ml of purified water was poured into a measuring container (1000 ml) and 1 g of carbapol gel was added, for its uniform distribution in water, constant stirring was carried out for 40 minutes. After that, 10 g of polymer was introduced into the flask with a mixture of carbopol and water, with constant stirring. Then, after thorough stirring, the resulting mixture was brought to a temperature of 40-45 ° C and 5 g of glycerol, 5 g of silicone and 1 g of triethanolamine were added, mixed well and allowed to cool to room temperature. After cooling, 400 ml of alcohol was added to the resulting mixture, 0.08 g of polyhexomethylene biguanide was added and the mixture was purified to the mark (1000 ml) with purified water. After obtaining a mixture of all components, the resulting gel was thoroughly mixed. The resulting composition was packaged in sealed containers until use.

Example 2. According to the described technology in example 1, per 1000 ml was taken: 9 g of polymer, 6 g of silicone, 350 ml of alcohol, 0.1 g of polyhexomethylene biguanide, 5 g of glycerol, 1 g of carbopol, 1 g of triethanolamine, the rest is purified water . The resulting composition was repacked in sealed containers before use.

Example 3. The Cow Mug (inv. No. 893) born in 2007 was launched on 07/19/2012. Immediately after the last milking of the cow, the udder's nipples were wiped with a dry napkin and the obtained product was applied to the nipples with a plastic cup. Immediately after the treatment of the nipples, the cow was assigned to the dead group. Subsequent daily monitoring of the state of the udder of the animal showed that the treated nipples have a transparent film that lasted on the nipples of the udder until calving. Immediately after calving, we examined the animal. The animal did not get mastitis. The reaction with the test diagnostician to the latent form of mastitis was negative.

Claims (1)

Means for the prevention of mastitis in dairy cows in the dry period, characterized in that the ingredients contain PVP / VA polymer, silicone, polyhexamethylene biguanide, ethyl alcohol, glycerin as an emollient, gelling agents - triethanolamine and carbopol and purified water in the following ratios of ingredients %:
polymer 9-10 silicone 5-6 ethanol 35-40 polyhexamethylene biguanide 0.008-0.01 glycerol 5 gelling agents - triethanolamine and carbopol 0.1 each purified water rest