RU2486905C1 - Method of treating mastitis in animals - Google Patents

Abstract

FIELD: medicine, pharmaceutics.

SUBSTANCE: invention refers to veterinary medicine, namely to medicines for treating mastitis in animals; a medicine for treating mastitis in animals contains the antibiotic tylosin, a gelling agent, water, with the gelling agent presented by carbopol and triethanolamine; the medicine further comprises Siberian fir essential oil and glycerol; an additional aseptic ingredient is plumepoppy juice prepared by pressing a vegetative part of a plant brought in a blossom time, in a step-loaded mechanical press with the first pressing force of 25-30 kN/cm², and the exposure of 5 minutes, the second pressing force of 55-60 kN/cm², and the exposure of 2.5 minutes; and the third pressing force of 80 kN/cm², and the exposure of 1 minute; the ingredients are related as, %; tylosin 0.05-1.0; Cok plumepoppy juice 0.05-1.0; glycerol 5.0-10.0; carbopol 0.15-0.20; triethanolamine 0.02-0.03; Siberian fir essential oil 0.1-0.3; water up to 100.

EFFECT: agent for treating mastitis in animals has a combined therapeutic and repellent effect and a prolonged shelf life.

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Description

The invention relates to the field of veterinary medicine, namely to means for the treatment of mastitis in animals.

Known drug for the prevention of mastitis in cows "Means for the prevention of mastitis in cows in the dry period", patent No. (11) 2342123, publication date 12/27/2008. This tool relates to veterinary medicine, has therapeutic and prophylactic properties for the treatment of mastitis in cows after transferring them to dead wood. The product in its composition contains iodismuthsulfamide and biopolymer. The biopolymer is obtained by the interaction of urea, formalin, glycerol, followed by the introduction of a solution of polyvinylpyrrolidone. The target product combines the properties of an antiseptic and a film coating. The product in its composition has urea and formalin - these unsafe substances can be irritating. Polyvinylpyrrolidone is used as a gelling agent - this synthetic compound can also have adverse effects.

Another drug used in veterinary medicine and pharmacology is the “Biogel-10” drug for the treatment of cows with mastitis and endometritis ”, patent No. (11) 2102074, publication date 01/20/1998. The Biogel-10 preparation for the treatment of cows with mastitis and endometritis contains 20% propolis alcohol extract, sodium carboxymethyl cellulose and distilled water. The disadvantage of this tool is that the alcohol tincture of the extract can dry the skin. No insect repellent.

The closest (prototype) to the claimed tool is the "Antibacterial composition for the treatment of animals", application for invention No. (11) 2002122440, publication date of application March 20, 2004. Antibacterial composition for the treatment of animals based on tylosin and its derivatives. As a gelling component, it contains phthalo gelatin. The product has a gel-like consistency. This product has healing properties, but the shelf life is very short, since gelatin and its derivatives are products of animal origin and, accordingly, are seeded by bacteria and quickly destroyed. Also, the drug does not have repellent properties.

The objective of the invention is to provide a gel-like agent for the treatment of mastitis in animals, which has both pronounced therapeutic and repellent effects and a prolonged shelf life.

The technical result is the strengthening of regenerative, anti-inflammatory, antiseptic and repellent properties. As an antiseptic, an antibiotic for animals is used, tylosin and mackley juice, obtained by pressing small-fruited mackley from a plant. As a binder, carbopol is used. As an auxiliary component, the product contains Siberian fir essential oil, which has the ability to repel insects, in particular flies.

This technical solution is achieved due to the fact that the agent for treating mastitis in animals, containing an antibiotic in the form of tylosin, a gelling component, water, characterized in that it contains carbopol and triethanolamine as a gelling component, additionally contains Siberian fir essential oil and glycerin, as an additional antiseptic component contains macklea juice, obtained by pressing the vegetative part of the plant, harvested during flowering, on a mechanical press using Niemi speed of a load to the first load compression force of 25-30 kN / cm ² and 5 minutes of exposure; the second compression force of 55-60 kN / cm ² , exposure 2.5 minutes; the third compression force of 80 kN / cm ² , exposure 1 minute, with the following ratio of components in the mixture,%:

Tylosin 0.05-1.0 MacLea juice 0.05-1.0 Glycerol 5.0-10.0 Carbopol 0.15-0.20 Triethanolamine 0.02-0.03 Fir Essential Oil 0.1-0.3 Water up to 100

Carbopol - gelling agent provides viscosity and the creation of surface films. Carbopol is a polymer of acrylic acid, its feature is the presence of cross "crosslinking" of the monomer. A polyhydric alcohol can be used as a crosslinking agent. All gel-like products and preparations containing carbopol have a prolonged shelf life, in contrast to the natural gel-forming components of the products.

Triethanolamine provides a gel-like structure, maintains a neutral environment in solution.

Glycerin has a softening effect, increases skin elasticity, a good moisture-retaining agent.

Fir essential oil, in addition to a pronounced local anti-inflammatory effect, has anti-inflammatory, immunomodulating, radioprotective and adaptogenic effects on the body, and also has the ability to repel insects.

The active substance of the drug is tylosin. It is a bacteriostatic active antibiotic designed specifically for use by animals. Tylosin acts on gram-positive and some gram-negative bacteria, including: E. coli, Bacillus anthracis, Pasteurella spp., Haemophilus spp., Leptospira spp., Stapylococcus spp., Streptococcus spp., Erysipelothrix spp., Corynema spppopium ., Chlamydia spp., Treponema spp. (Brachispira) and others. By the degree of exposure to the body refers to low hazard.

Excipient is macklea juice small-fruited. Juice has good antiseptic properties, is a natural product isolated from the aerial part of the plant - small-fruited mackley. The plant is rich in alkaloids - sanguinarine and chelirithrin, which have a pronounced antiseptic effect. Small-fruited mackley juice obtained by pressing the vegetative part of a plant harvested during flowering on a mechanical press using a step load in the form of loading with a first compression force of 25-30 kN / cm ² and holding for 5 minutes; the second compression force of 55-60 kN / cm ² , exposure 2.5 minutes; the third compression force of 80 kN / cm ² , exposure 1 minute, which increases the degree of extraction of biologically active substances from the plant.

The technology for preparing the claimed anti-mastitis agent in animals in the form of a gel-like liquid is as follows: the required amount of carbopol and the calculated amount of water are mixed and left to swell at room temperature for 40 minutes until a homogeneous state is obtained. Then glycerin is added and the resulting gel-like mixture of water, carbopol and glycerin is heated to 40-45ºC with stirring (50-60 rpm), then the specified amount of tylosin, triethanolamine and maklei juice is added. Stirring is carried out for another 5-10 minutes. The heat is removed, then Siberian fir essential oil is introduced. After that, the mass is cooled in a bath of cold water.

The tool has anti-inflammatory, emollient, antiseptic properties. Has the ability to repel insects. To confirm the claimed properties, we performed experiments, which are shown in table 1.

Table 1 Examples of formulations developed tools and prototype Recipe Components Mass fraction of components, in% The claimed tool Prototype Experience No. 1 Experience No. 2 Experience No. 3 Experience No. 4 Experience No. 5 tylosin 0.05 0.53 1,0 5,0 10.0 maklei juice 0.05 0.53 1,0 - - glycerol 5,0 7.50 10.0 - - carbopol 0.15 0.18 0.20 - - triethanolamine 0.02 0,025 0,03 - - fir essential oil 0.1 0.2 0.3 - - phthalo gelatin - - - 1,0 2.0 water up to 100 up to 100 up to 100 up to 100 up to 100

Preparation of 100 g of the developed product and prototype

Test No. 1: 0.15 mg of carbopol and 94.68 cm ^{3 of} water are mixed and allowed to swell at room temperature for 40 minutes until a homogeneous state is obtained. Then add 5.0 cm ³ glycerol and the resulting gel-like mixture of water, carbopol and glycerol is heated to 40-45ºC with stirring (50-60 rpm), then add 0.05 mg tylosin, 0.02 cm ³ triethanolamine, 0, 05 cm ³ juice of macklea small-fruited. Stirring is carried out for another 5-10 minutes. The heat is removed, then 0.1 cm ³ of Siberian fir essential oil is introduced. After that, the mass is cooled in a bath of cold water.

Test No. 2: 0.18 mg of carbopol and 91.57 cm ^{3 of} water are mixed and allowed to swell at room temperature for 40 minutes until a homogeneous state is obtained. Then 7.50 cm ^{3 of} glycerol is added and the resulting gel mixture of water, carbopol and glycerin is heated to 40-45 ° C with stirring (50-60 rpm), then 0.53 mg of tylosin, 0.025 cm ^{3 of} triethanolamine, 0.53 cm are added. ³ Macleia juices. Stirring is carried out for another 5-10 minutes. The heat is removed, then 0.2 cm ³ of Siberian fir essential oil is introduced. After that, the mass is cooled in a bath of cold water.

Test No. 3: 0.20 mg of carbopol and 88.47 cm ^{3 of} water are mixed and allowed to swell at room temperature for 40 minutes until a homogeneous state is obtained. Then add 10.0 cm ^{3 of} glycerol and the resulting gel-like mixture of water, carbopol and glycerol is heated to 40-45ºC with stirring (50-60 rpm), then add 1.0 mg of tylosin, 0.03 cm ^{3 of} triethanolamine, 1, 0 cm ³ juice maklei small fruited. Stirring is carried out for another 5-10 minutes. The heat is removed, then 0.3 cm ³ of Siberian fir essential oil is introduced. After that, the mass is cooled in a bath of cold water.

The technology for preparing a prototype sample is similar to the technology for preparing the invention, only phthaloyelatin is used as a gelling agent.

Test No. 4: 1.0 mg of phthaloyl gelatin is mixed with 94.00 cm ^{3 of} water, then 5.0 mg of tylosin is added.

Run No. 5: 2.0 mg of phthaloyl gelatin is mixed with 88.00 cm ^{3 of} water, then 10.0 mg of tylosin is added.

Laboratory studies were conducted to determine the anti-inflammatory effect and the effect on regenerative processes, antimicrobial activity was analyzed, and the repellent properties of the drug were determined.

Determination of anti-inflammatory action and the effect of the drug on regenerative processes

The anti-inflammatory effect of the drugs was determined on guinea pigs with a live weight of 400-500 g. Four groups of three guinea pigs in each group were formed, of which two experimental and two control groups.

The animals of the experimental and control groups applied three drops of xylene to the skin area, after depilation, in the back, which was rubbed into the skin with a glass rod for two minutes to cause dermatitis. After one hour, and then every day, 1 time per day, 0.1 ml of the test preparation was applied to a xylene-burned area of skin with a diameter of 10 mm.

During the first days after thermal exposure and further treatment with the agent, skin hyperemia, slight swelling in the affected area were detected. By the end of the second day, the signs of inflammation disappeared, on the 3-5th day there were no signs of dermatitis.

In animals of the control group in which the prototype was used, during the first day at the places of chemical exposure, hyperemia and puffiness were revealed, most pronounced 6-10 hours after the irritating effect of the chemical agent. On the second day, more pronounced signs of inflammation were noted in the affected area (hyperemia, swelling, soreness); on the third day, itching; by 4-5 days, healing took place on 8-10 days.

The data obtained allow us to conclude that the drug has a pronounced anti-inflammatory effect.

A study of the effect of the agent on the course of regenerative processes was carried out in a comparative aspect of the model of experimentally reproduced infected muscle-skin wounds in 20 young white rats weighing 80-100 g, taken from the same litters and distributed in the experimental and control groups. Muscle-skin wounds were inflicted without observing aseptic rules. A 2 × 2 cm area was carefully cut on the back of all animals. Then, a contour of a wound 1 cm in diameter was drawn with a stencil (the sharp edges of the cork piercer) and a skin and muscle flap was cut with eye scissors.

The drug was applied to ten experimental rats on the wound surface, and the prototype was applied to 10 control rats.

After that, the wounds were covered with a gauze napkin, on top of which a gauze flap was applied, and its edges were glued to the animal's hair with a thick collodion. For a more reliable fixation of the bandage, it was strengthened with a bandage. Dressings were performed every day.

The healing progress of experimental wounds was judged by the following indicators: the time of granulation in the wound, the closure of the bottom of the wound by granulation, the quality of granulation, the course of epithelization, the condition of the tissues around the wound. As observations showed, in animals of the experimental group, wound healing (performing granulation, the disappearance of hyperemia, swelling and pain, epithelization with scar formation) took place on average six days earlier than in animals of the control group.

The data obtained allow us to conclude that the drug has a beneficial effect on regenerative processes, stimulating them.

Antimicrobial activity of the drug

The antimicrobial properties of the drug were tested by diffusion into agar using wells.

As test cultures used daily field purified isolates of microorganisms. The results of the studies are shown in table 2.

Analyzing the results of studies to determine the sensitivity of opportunistic microflora to drugs, we establish that the experienced drug most actively impedes the growth of microflora.

table 2 Determination of the sensitivity of microorganisms to test drugs Preparations Growth retardation zone in mm St. aureus Str. pyogenes E.coli Shigella boudi The drug with tylosin and makleia 29.5 31.5 fifteen 38 The drug with tylosin 35.7 20.3 11.2 15.6 Prototype 5.3 7.5 - -

The activity of the effect of the drug with tylosin and makleia on microorganisms was analyzed. The results of laboratory tests are presented in table 3.

Table 3 The effect of the drug with tylosin and makleia on the activity of growth of microorganisms The name of the microorganisms The zone of growth inhibition of microorganisms, mm I. enterocolitica 30.5 R. mirabilis - Ps. aeroginosa 15,5 St. aureus 29.5 K.L pneumoniae delay St. warreri 49.5 St. huicus 43.5 St. epidermidis 33 St. hominis 29th Sh.boudi 38 Sh. desenteria thirty

Repellent properties of the drug

In the experiment, 10 cows were used, which were divided into 2 groups of 5 animals each. The cows of the first group (experimental) were applied the experimental preparation, the animals of the second group (control) - the prototype. The tested preparations were applied to the udder nipples immediately after milking by lowering the udder nipple into a special glass for processing the nipples.

As a result of observation of animals, it was found that the drug has a repellent property for 3-3.5 hours, while a control agent (prototype) does not have such an effect.

The results of all experiments are presented in table 4.

Table 4 Experiment Results Recipe Components Examples of the limit of funds per 100 g Prototype Experience No. 1 Experience No. 2 Experience No. 3 Experience No. 4 Experience No. 5 Anti-inflammatory effect Healing on: + + + + + 3-5 day 3-5 day 3-5 day 8-10 days 8-10 days Impact on regenerative processes + + + for 6 days for 6 days for 6 days + + before before before Antimicrobial activity + + + ± ± Shelf life 1,5 years 1,5 years 1,5 years 1 year 1 year Repellent properties + + + - - + - properties detected; - - properties not found

According to the obtained laboratory experimental data, one can judge the results of this invention. The technical result of the invention was that an anti-mastitis agent based on tylosin and macklea for the treatment of mastitis in animals, which contains carbopol as a gel-forming substance, has beneficial effects on regenerative processes, stimulating them, actively fights against microorganisms, in comparison with other drugs. After using the product, the elasticity of the skin increases, the product has a repelling property against flies, heals, softens the skin. Based on the foregoing, technical goals have been achieved.

Claims (1)

An agent for treating mastitis in animals, containing an antibiotic in the form of tylosin, a gel-forming component, water, characterized in that it contains carbopol and triethanolamine as a gel-forming component, additionally contains Siberian fir essential oil and glycerin, and contains macklea juice, as an additional antiseptic component, obtained by pressing the vegetative part of the plant, harvested during flowering, on a mechanical press using a step load in the form of loading with the first compression ratio 25-30 kN / cm ² and exposure for 5 min; the second compression force of 55-60 kN / cm ² holding 2.5 min; the third compression force of 80 kN / cm ² , holding for 1 min, with the following ratio of components in the mixture,%:
Tylosin 0.05-1.0 MacLea juice 0.05-1.0 Glycerol 5.0-10.0 Carbopol 0.15-0.20 Triethanolamine 0.02-0.03 Fir Essential Oil 0.1-0.3 Water up to 100