RU2682889C1 - Means for prevention and treatment of cows mastitis and method of application thereof - Google Patents

Abstract

FIELD: agriculture.

SUBSTANCE: group of inventions relates to agriculture, in particular to veterinary medicine, and is a means for the prevention and treatment of cows mastitis, which is an aerosol with a particle size of less than 100 microns and a pH of 6.5–7.5, containing a soluble salt of rare-earth element selected from cerium nitrate or lanthanum chloride, glycerin, triethyleneglycol, ethyl alcohol, potassium hydroxide, hyaluronic acid, pine nut oil, vitamins A, C, E, F and distilled water, and the components in the product are in a certain ratio in wt.%, as well as a method for the prevention and treatment of cows mastitis, consisting in applying the above-mentioned at least 1 time a day after the completion of milking by 2-3 sprays.

EFFECT: invention provides high efficacy in the prevention and treatment of mastitis, safe, stable during storage and more convenient to use.

3 cl, 4 tbl, 5 ex

Description

The invention relates to agriculture, in particular to veterinary medicine, and can be used to treat mastitis in animals.

Mastitis is understood to mean inflammation of the mammary gland in mammals that develops under the influence of environmental factors, biological factors, and environmental factors (Studentsov A.P., Shipilov V.S. et al. Veterinary obstetrics, gynecology and reproduction biotechnology. M .: Kolos, 1999, p. 357-369; Ivashura A.I. System of measures to combat mastitis of cows. - M.: Rosagropromizdat, 1991, p. 168-201).

Inflammation of the udder in Russia, as in many countries of the world with developed dairy cattle breeding, takes a leading place among cow diseases and causes great economic damage. It is recorded in 30, and in enzootic cases, up to 80 percent of dairy cows. The economic damage caused by mastitis consists of losses in milk productivity, deterioration of the nutritional and technological properties of milk, as well as a reduction in the life of cows, incidence of calves, treatment costs and other factors. Therefore, the development of the most effective means and methods of combating mastitis in cows and their introduction into the technology of dairy cattle breeding is a great reserve in increasing livestock productivity and food quality for the population.

Environmental factors contributing to the onset and development of mastitis include mechanical, thermal and chemical. The main factors are the technical imperfection of milking machines and the violation of machine milking technology, as well as the violation of optimal conditions for keeping and feeding cows.

In case of violation of veterinary and sanitary rules for the care of milking units and milking hygiene, as well as a decrease in the overall stability of the animal and mammary gland, colonization of the nipple apex and the external opening of the nipple canal occurs with microorganisms. Then microorganisms penetrate into the nipple and sucker tanks (sinuses) and cause an infectious process of varying severity from subclinical to clinically severe inflammation of various forms. The most common causative agents of mastitis are gram-positive microorganisms (Staphylococcus aureus, Streptococcus agalactiac, etc.), as well as gram-negative (E. coli, Klebsiella, etc.) and fungi. Therefore, in the complex of measures to combat mastitis, the main importance is attached to the use of special anti-inflammatory drugs, which have an antibacterial effect against pathogens of the udder.

For the treatment of mastitis, drugs containing antiseptic agents (iodine, rivanol, etc.), chemotherapeutic agents, such as sulfa drugs, antibiotics, nitrofurans, quinolones, etc. are used (RU 2180559, 2002). In particular, iodine-based preparations (septogel, lasin, synthiod), ichthyol and formalin solutions, mercury preparations (RU 2180558, 2002; RU 2239422, 2004; Gavrish V.G., Egunova A.V., Semenov S.V., Novikova S.V. Septogel for treating cows with mastitis // Veterinary Medicine, 2000, 6, p. 33-36). So, it is supposed to be used against mastitis (RU 2401657, 2010) by intra-administration of a medicinal product containing 40% formalin, succinic acid, Dorogov stimulant and gel-forming agent. The drug is administered at a dose of 10.0 ml daily until complete recovery.

The disadvantage of such drugs is the indiscriminate effect on microorganisms, which leads to the death of beneficial microorganisms, as well as the fact that when exposed to them, irritation of the epithelium of the milk passages and alveoli is often noted, which can lead to hypogalactia and agalactia of the treated lobes.

Such antibiotic-based chemotherapeutic drugs are known as Mastisan A, which contains potassium or sodium salt of benzylpenicillin, streptomycin, norsulfazole or sulfadimesin suspended in vegetable oil; Mastisan B for the treatment of mastitis, containing neomycin sulfate, sulfadimezin, methyluracil, liquid paraffin and polyglycerol distirate; Mastisan E containing erythromycin, sulfadimesin, polyethylsiloxane liquid (PES) with an emulsifier as an oil base; Masticide containing penicillin potassium or sodium salt, streptomycin sulfate, norsulfazole, sulfadimezin, sunflower or petrolatum oil and an emulsifier as an oil base, as well as mastaerosol, difurol-A, menersin A, mastivalen, lactopen, notil, aerodite, aerodite, others, also containing antibiotics, sulfonamides and nitrofuran derivatives (Klenova I.F., Yaremenko N.A. Veterinary preparations in Russia. Reference book. - Moscow: Selkhozizdat, 2000, 544 pp .; Klenova I.F., Ilyukhina I. N., Pisanova L.A. Foreign veterinary medicine arata in Russia. Reference book. M: "Evers Company, 1999, 314 p .; Veterinary drugs. Reference book / Under the editorship of AD Tretyakov. - M.: Agropromizdat, 1988; Goncharov V.P., Karpov V.A. et al., Prevention and treatment of animal mastitis - M .: Rosselkhozizdat, 1987, 174 pp. Etc.)

A common disadvantage of known drugs is the low therapeutic efficacy, the emergence of antibiotic-resistant strains during repeated use of antibiotics, and the instability of drugs during storage (antibiotics in the aqueous phase quickly lose their activity, sulfonamides precipitate, most drugs in suspension form exfoliate, and vegetable oils are oxidized).

Closest to the claimed invention is the composition "Vilpran-vet" (RU 2605631, 2016) for the prevention of mastitis in cattle, containing glycerin, cerium nitrate, triethylene glycol, polyethylene glycol, milk thistle oil, sea buckthorn oil, ethyl alcohol, furatsilin, potassium hydroxide, purified water in the following ratio of ingredients, wt. %: glycerol 6.0-7.0; cerium nitric acid 2.0-2.5; triethylene glycol 50.0-55.0; polyethylene glycol 5.0-25.0; milk thistle oil 1.0-2.0; sea buckthorn oil 2.0-4.0; ethyl alcohol 4.0-6.0; furatsilin 0.2-0.4; potassium hydroxide 0.5-0.6; purified water - the rest. "Vilpran-vet" was a uniform mass from white to cream in color, which is easily squeezed out of the tube and then applied to the skin of the udder. The composition is used by lubricating the udder and nipples of the animal after milking 1-2 times a week.

A significant drawback of the composition was the short duration of action associated with insufficient bacteriostatic effect on microorganisms, as well as instability during storage, leading to delamination of the ointment and the loss of its effectiveness. The disadvantage of the method of applying the ointment is the unevenness of its application to the surface of the udder, determined by the subjective factor (features of the milkmaid), the need for prolonged contact of the drug until completely absorbed, insufficient penetration of the ointment into the nipple channels.

The problem solved by the authors was the creation of a more effective composition, namely, with increased bacteriostatic characteristics and penetrating ability, as well as allowing the use of a more reliable and convenient method of applying the product to the udder of an animal in the form of an aerosol.

The technical result was achieved by creating a tool containing, along with the soluble salt of the rare-earth element (hereinafter CPM), glycerin, triethylene glycol, ethyl alcohol, potassium hydroxide and water, additional hyaluronic acid, pine nut oil and vitamins in the following ratio of ingredients, wt. %:

triethylene glycol 45.0-60.0 rare earth salt 1.2-1.8 glycerol 15.0-20.0 ethanol 3.0-4.5 potassium hydroxide 0.3-0.5 hyaluronic acid 2.0-6.0 pine nut oil 0.7-1.2 vitamins 1.0-4.0 distilled water - the rest.

As salts of rare-earth metals, the product contains soluble salts of lanthanum or cerium, for example, nitrates or chlorides, and as vitamins it contains vitamins A, E, F, C. Optimum vitamins in the product are used in the following concentrations (% of mass): vitamin A - 0.05-0.1; a mixture of vitamins E and F - 1.3-1.5, vitamin C - 0.6-1.2.

The resulting product received the code name "VILMAST". The main feature of the claimed agent is the replacement of polyethylene glycol with hyaluronic acid at concentrations several times higher than that commonly used in ointments, which provided deeper penetration into the tissues of the udder and fixation of rare-earth metal ions, which provide a protective bactericidal and wound healing effect in the nipple channels and udder tanks, especially in the most dangerous period for infection by microorganisms is 2 hours after milking, when the channels remain open.

At the same time, adjusting the concentration of other components of the composition, as shown by experiments, significantly increased the stability of the composition, both in the form of an aerosol and during storage. Exceeding the concentration of ingredients such as triethylene glycol, glycerin and ethyl alcohol outside the declared concentrations violates its stability. The decrease in the concentration of CPM, pine nut oil and vitamins relative to the claimed reduces the effectiveness of the drug, their use in a higher concentration is not economically feasible. The introduction of potassium hydroxide is carried out in a concentration that provides a neutral pH of the composition.

The technical result with respect to the method was the prophylaxis and treatment of mastitis with the above-described VILMAST in the form of an aerosol with a particle size of less than 100 microns. "VILMAST" for therapeutic purposes is applied to the tissue of the udder of the animal at least once a day by 2-3 sprayings immediately after milking (when the nipple channels are still open).

VILMAST was obtained by dissolving CPM in triethylene glycol at 40-60 ° C, after which hyaluronic acid, glycerin, pine nut oil, vitamins are added with vigorous stirring. The resulting mixture for further use is placed in aerosol cans with a nozzle adjusted to create an aerosol with a particle size of less than 100 microns.

VILMAST studies have shown its high efficiency in relation to bactericidal, bacteriostatic and wound healing effects, as well as complete harmlessness and physiology for the animal. The drug does not affect the properties of milk and dairy products obtained on its basis. Compared with traditional drugs, the treatment time for serous mastitis decreases on average by half. Shelf life of the product without changing properties is at least 3 years.

The proposed prevention and treatment scheme is economical, convenient to use, in particular, it allows you to handle hard-to-reach spots and facilitates the work of milkmaids in processing the udder of lactating cows.

The essence and advantages of the claimed group of inventions are illustrated by the following examples.

Example 1. Anti-mastitis means "VILMAST" was prepared as follows. A portion of cerium nitrate or lanthanum chloride was dissolved in triethylene glycol at 40-60 ° C for 2 hours. Then, with vigorous stirring, hyaluronic acid, glycerin, pine nut oil, and vitamins were added. The resulting mixture was cooled and used for preliminary tests on bacterial cultures or placed in aerosol cans with a nozzle adjusted to create an aerosol with a particle size of less than 100 microns for field testing.

By varying the number of ingredients, compositions were obtained whose composition is shown in table 1.

*% - the content of lanatan or cerium in the cream;

** distilled water is used.

The pH of all of these compositions is 6.5-7.5.

Example 2. The study of the stability of the compositions was carried out according to their bactericidal activity against bacteria.

A study of the conservation of bacteriostatic activity of the compositions was carried out on a solid nutrient medium. The product was diluted with physiological saline, introduced into preheated meat-peptone agar and poured into Petri dishes. A test culture of microorganisms Staphylococcus aureus ATCC 6538, Esherichia coli M17 at a concentration of 500 microbial cells was seeded. 48 hours after incubation of the cultures at 37 ° C., the number of colonies grown was counted. As the means used the compositions shown in table 1, stored in refrigerators at a temperature of + 4 ° C for a specified time. As control, we used the Vilpran-vet compound (the closest analogue). The results are shown in table 2.

Test data showed that all VILMAST compositions have biocidal characteristics that are stored during storage in the refrigerator for a year. In this case, the drug analogue during storage quickly loses its activity. The best results were obtained on the compositions VILMAKS 3 and VILMAKS 4.

Example 3. The study of the bacteriostatic activity of compositions 3 and 4 was carried out on a solid nutrient medium. The product was diluted with physiological saline, introduced into preheated meat-peptone agar and poured into Petri dishes. A test culture of microorganisms Staphylococcus aureus ATCC 6538, Staphylococcus agalactia ATCC 12386 at a concentration of 500 microbial cells was seeded. 48 hours after incubation of cultures at a temperature of 37 ° C, the number of colonies grown. The results are shown in table 3.

The results obtained indicate strong bacteriostatic properties of the studied compositions, as well as the preservation of these properties for a long time.

Example. 4. Bacteriostatic activity was checked based on the analysis of swabs on the surface of the udder for the presence of pathogenic microbes on the example of groups of 10 animals in each group. The results are shown in table 4

Example 5. In the farm of the Nizhny Novgorod region on a livestock of 210 cows, the drug VILMAST 3 was tested for 9 months. 3 groups of 70 animals were formed. In the first group, the cows were treated with an aerosol agent after each milking, in the second group, the treatment was carried out once a day after the first milking, in the third group, the treatment was not carried out. In the first and second groups, no cow had mastitis. In the third group, 10 cows showed the onset of serous disease of the udder. After transferring the sick cows to the regimen of the first group, after 7 days the signs of the disease disappeared.

In all animals of groups 1 and 2, an improvement in the condition of the skin of the udder and nipples was noted, the healing of lesions of the skin of the udder was accelerated, the skin became more elastic and elastic. Milkmaids noted greater usability of the drug in the form of an aerosol.

Studies have shown that the claimed tool is highly effective in the prevention and treatment of mastitis, safe, stable during storage and more convenient to use.

Claims (5)

1. The tool for the prevention and treatment of mastitis in cows, which is an aerosol with a particle size of less than 100 microns and a pH of 6.5-7.5, containing a soluble salt of the rare earth element selected from cerium nitrate or lanthanum chloride, glycerin, triethylene glycol, ethyl alcohol , potassium hydroxide, hyaluronic acid, pine nut oil, vitamins A, C, E, F and distilled water in the following ratio of ingredients, wt.%: triethylene glycol 45.0-60.0 rare earth salt selected from cerium nitrate or lanthanum chloride 1.2-1.8 glycerol 15.0-20.0 ethanol 3.0-4.5 potassium hydroxide 0.3-0.5 pine nut oil 0.7-1.2 hyaluronic acid 2.0-6.0 vitamins A, C, E, F 1.0-4.0 distilled water rest 2. The tool according to p. 1, characterized in that it contains vitamins in the following proportions, wt. %: vitamin A 0.05-0.1 a mixture of vitamins E and F 1.3-1.5 vitamin C 0.6-1.2 3. A method for the prevention and treatment of mastitis in cows, which consists in applying the product according to claim 1 at least 1 time per day after milking is completed by 2-3 sprays.